Overview
- Presents methods for all stages of the plant metabolomics workflow
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 860)
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About this book
Estimation of the metabolite complement of plant material involves a wide range of techniques and technologies and that breadth continues to increase. Metabolomics research typically involves multiple sites for material preparation and analysis and most investigations are “high throughput”, meaning that chemical analysis of sample sets are inevitably carried out over an extended period of time. In, Plant Metabolomics: Methods and Protocols expert researchers in the field detail many of the stages which are now commonly used to study plant metabolomics workflow. Stages of this workflow, up to and including the statistical analysis, accurate and detailed collection of meta-data are also essential for good process management, to satisfy reporting requirements and to ensure wider interpretability and reuse results.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Through and intuitive Plant Metabolomics: Methods and Protocols, seeks to aid scientists in the further study of the methods for all the stages of the plant metabolomics workflow.
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Keywords
Table of contents (18 protocols)
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Material Preparation
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Chemical Analysis Approaches
Reviews
From the reviews:
“The chapters presented in this volume stay true to the Methods and Protocols series, with a short piece of background information, a highly detailed step-by-step protocol, and additional practical information that will allow beginners in the field to set up repeatable, high-quality metabolomics experiments. … this is an excellent addition to the series and can provide muchneeded support, especially to scientists starting in the growing field of plant metabolomics.” (Alexandre Foito, Annals of Botany, Vol. 113 (7), April, 2014)
“The book ‘Plant Metabolomics. Methods and Protocols’ is fresh in ideas and a useful manual for scientists, researchers, scholars, students and the wider readership interested in plant metabolism and metabolite molecules. … The purpose of this volume is to provide information on basic practical questions concerning experimental work with metabolomics. … The book is a mix between a scientific treatise and a laboratory manual. As such it is an excellent one.” (Tadeusz Aniszewski, Plant Science Bulletin, Vol. 58 (4), 2012)Editors and Affiliations
Bibliographic Information
Book Title: Plant Metabolomics
Book Subtitle: Methods and Protocols
Editors: Nigel W. Hardy, Robert D. Hall
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-594-7
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2012
Hardcover ISBN: 978-1-61779-593-0Published: 17 February 2012
Softcover ISBN: 978-1-4939-5748-4Published: 23 August 2016
eBook ISBN: 978-1-61779-594-7Published: 22 February 2012
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIII, 340
Topics: Plant Sciences, Plant Physiology, Computer Appl. in Life Sciences