Overview
- Brings together the most important and current techniques in chloroplast research through the unifying lens of the Arabidopsis model system
- Features subjects like protein aspects, omics studies, and photosynthesis and biochemical analysis
- Includes expert tips and implementation advice to guarantee ideal experimental results
Part of the book series: Methods in Molecular Biology (MIMB, volume 775)
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About this book
Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable. The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid. In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.
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Keywords
Table of contents (22 protocols)
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Multiprotein Complexes and Protein-Protein Interactions
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Omics and Large-Scale Analyses
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Proteomics and Suborganellar Fractionation
Editors and Affiliations
Bibliographic Information
Book Title: Chloroplast Research in Arabidopsis
Book Subtitle: Methods and Protocols, Volume II
Editors: R. Paul Jarvis
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-61779-237-3
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2011
Hardcover ISBN: 978-1-61779-236-6Published: 23 August 2011
Softcover ISBN: 978-1-4939-6289-1Published: 23 August 2016
eBook ISBN: 978-1-61779-237-3Published: 23 August 2011
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 432
Number of Illustrations: 56 b/w illustrations, 5 illustrations in colour
Topics: Plant Sciences, Plant Biochemistry