Overview
- Provides an easily accessible reference volume for investigating mitosis put together by leading researchers in the field
- Presents approaches in both mammalian and chicken cell systems as well as in model single-cell and multi-cellular organisms
- Covers a broad range of biochemical, genetic and cell biological methods focused on dissecting specific mitotic processes
- Contains up-to-date live cell imaging-based approaches to study dynamic mitotic events
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 545)
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About this book
With the advent of modern methodology, the phenomenal complexity of the protein components and regulatory steps involved in mitosis has become approachable. In Mitosis: Methods and Protocols, experts in the field provide an up-to-date collection of methods and approaches that are used to investigate the mechanism of mitosis at the molecular level. Ideal for both experienced and novice scientists, the volume includes step-by-step methods covering inactivation of genes of interest, depletion of proteins of interest, biochemical and microscope-based techniques, and procedures to monitor and measure key mitotic processes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Mitosis: Methods and Protocols combines imaging, biochemical, and genetic methodologies in order to best guide researchers in their attempts to better understand the crucial processes of mitotic regulation.
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Table of contents (20 protocols)
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Editors and Affiliations
Bibliographic Information
Book Title: Mitosis
Book Subtitle: Methods and Protocols
Editors: Andrew D. McAinsh
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-60327-993-2
Publisher: Humana Totowa, NJ
eBook Packages: Springer Protocols
Copyright Information: Humana Press 2009
Hardcover ISBN: 978-1-60327-992-5Published: 29 June 2009
Softcover ISBN: 978-1-62703-850-8Published: 24 November 2014
eBook ISBN: 978-1-60327-993-2Published: 28 May 2009
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 345
Topics: Cell Biology, Developmental Biology, Cell Cycle Analysis, Gene Expression