Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
- Includes supplementary material: sn.pub/extras
Part of the book series: Methods in Molecular Biology (MIMB, volume 1366)
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About this book
This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.
The chapter 'Bioinformatics Analysisof Estrogen-Responsive Genes' is open access under a CC BY 4.0 license.
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Keywords
Table of contents (47 protocols)
Editors and Affiliations
About the editor
Bibliographic Information
Book Title: Estrogen Receptors
Book Subtitle: Methods and Protocols
Editors: Kathleen M. Eyster
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-3127-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2016
Hardcover ISBN: 978-1-4939-3126-2Published: 20 November 2015
Softcover ISBN: 978-1-4939-5006-5Published: 23 August 2016
eBook ISBN: 978-1-4939-3127-9Published: 19 November 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVI, 576
Number of Illustrations: 41 b/w illustrations, 87 illustrations in colour
Topics: Biochemistry, general, Endocrinology