Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1347)
Access this book
Tax calculation will be finalised at checkout
Other ways to access
About this book
This volume mirrors the holistic feature of whole genome amplification (WGA) technology by combining reviews, detailed basic methods and advanced sample workflows. The first part of the book covers an overview of the development of WGA techniques throughout recent years including general considerations on bias in WGA, possible sample pre-enrichment strategies and how to run a single-cell lab. The second part focuses on major WGA methods and protocols that allow the assessment of WGA product quality. The final chapters contain advanced protocols and address issues such as sample preparation using laser-micro dissection; WGA from partially degraded DNA (formalin-fixed paraffin embedded samples); circulating tumor cells; and ancient samples. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Whole Genome Amplification: Methods and Protocols will serve as a rich source of detailed information and inspiration and will help researchers, both new and experienced, yield successful results.
Similar content being viewed by others
Keywords
Table of contents (18 protocols)
Editors and Affiliations
About the editor
Bibliographic Information
Book Title: Whole Genome Amplification
Book Subtitle: Methods and Protocols
Editors: Thomas Kroneis
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-2990-0
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-2989-4Published: 16 September 2015
Softcover ISBN: 978-1-4939-4846-8Published: 22 October 2016
eBook ISBN: 978-1-4939-2990-0Published: 16 September 2015
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: X, 284
Number of Illustrations: 5 b/w illustrations, 13 illustrations in colour
Topics: Human Genetics