Overview
- Includes fully updated, cutting-edge methods and protocols for nuclear reprogramming
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1222)
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About this book
Nuclear Reprogramming: Methods and Protocols, Second Edition includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Nuclear Reprogramming: Methods and Protocols, Second Edition will be of interest not only to cloners but also to researchers concerned with studying the development of embryos.Similar content being viewed by others
Keywords
Table of contents (20 protocols)
Editors and Affiliations
Bibliographic Information
Book Title: Nuclear Reprogramming
Book Subtitle: Methods and Protocols
Editors: Nathalie Beaujean, Hélène Jammes, Alice Jouneau
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-1594-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media New York 2015
Hardcover ISBN: 978-1-4939-1593-4Published: 07 October 2014
Softcover ISBN: 978-1-4939-5441-4Published: 23 August 2016
eBook ISBN: 978-1-4939-1594-1Published: 06 October 2014
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 285
Number of Illustrations: 25 b/w illustrations, 43 illustrations in colour
Topics: Cell Biology, Genetic Engineering