Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2763)
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About this book
This volume explores the latest advancements in mucin research. The chapters in this book are organized into 8 parts and cover a wide range of topics such as mucin extraction, isolation, physicochemical property analysis, and experimental methods. The chapters also discuss the origins of mucins in jellyfish, feces, saliva and salivary glands, bronchi, stomach, and cervical tract; organic synthesis of peptides glycosylated at specific sites; analysis of mucin gene expression and methylation-specific electrophoresis of genes; imaging of mucin networks by AFM; and experimental methods using supported molecular matrix electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Mucins: Methods and Protocols is a valuable resource for researchers who want to obtain in-depth insight on how to more thoroughly conduct mucin research in their laboratories.
Keywords
Table of contents (35 protocols)
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Mucin-Hydrogel and Physicochemical Properties
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Front Matter
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Back Matter
Editors and Affiliations
Bibliographic Information
Book Title: Mucins
Book Subtitle: Methods and Protocols
Editors: Akihiko Kameyama
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-3670-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024
Hardcover ISBN: 978-1-0716-3669-5Published: 14 February 2024
Softcover ISBN: 978-1-0716-3672-5Due: 27 February 2025
eBook ISBN: 978-1-0716-3670-1Published: 13 February 2024
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 417
Number of Illustrations: 1 b/w illustrations
Topics: Biological Techniques, Protein Science, Immunology