Overview
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2476)
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About this book
This detailed new edition collects cutting-edge laboratory protocols, techniques, and applications in use by some of the leading international experts in the broad field of chromosome architecture. The book emphasizes the increasing physiological relevance of chromosome architecture investigation, manifest both through application of more complex bottom-up assays in vitro as well as through maintaining the native physiological context through the investigation of living, functional cells. In addition, the chapters reflect the dramatic improvement in the length scale of precision by utilizing single-molecule approaches, both for imaging the DNA content of chromosome and proteins that bind to DNA as well as using methods that can controllably manipulate single DNA molecules, and the use of advanced computational methods and mathematical analysis is also featured. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.
Authoritative and up-to-date, Chromosome Architecture: Methods and Protocols, Second Edition is an ideal guide for researchers working in this dynamic area of study.
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Keywords
Table of contents (21 protocols)
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Front Matter
Editors and Affiliations
Bibliographic Information
Book Title: Chromosome Architecture
Book Subtitle: Methods and Protocols
Editors: Mark C. Leake
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2221-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-2220-9Published: 31 May 2022
Softcover ISBN: 978-1-0716-2223-0Published: 01 June 2023
eBook ISBN: 978-1-0716-2221-6Published: 30 May 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 330
Number of Illustrations: 9 b/w illustrations, 76 illustrations in colour
Topics: Cell Biology