Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2461)
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About this book
This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.
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Keywords
Table of contents (16 protocols)
Editors and Affiliations
Bibliographic Information
Book Title: Directed Evolution
Book Subtitle: Methods and Protocols
Editors: Andrew Currin, Neil Swainston
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2152-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-2151-6Published: 22 June 2022
Softcover ISBN: 978-1-0716-2154-7Published: 23 June 2023
eBook ISBN: 978-1-0716-2152-3Published: 21 June 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 278
Number of Illustrations: 5 b/w illustrations, 58 illustrations in colour
Topics: Biotechnology, Human Genetics