Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible result
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2378)
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About this book
This volume is divided in six section covering the most experimental approaches involved in the study of the unfolded protein response (UPR) pathway. Chapters detail determination of unfolded protein levels, methods to study UPR signal transmission, analysing the outcomes of the UPR pathway activation, UPR studies in mammalian models, UPR in alternative models, and UPR and disease. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, The Unfolded Protein Response: Methods and Protocols aims to describe key methods and approaches used in the study of the UPR pathway and its complex cellular implications.
Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via springerlink.bibliotecabuap.elogim.com.
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Keywords
Table of contents (20 protocols)
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Determination of Unfolded Protein Levels
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Methods to Study UPR Signal Transmission
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Analysing the Outcomes of the UPR Pathway Activation
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UPR Studies in Mammalian Models
Editors and Affiliations
Bibliographic Information
Book Title: The Unfolded Protein Response
Book Subtitle: Methods and Protocols
Editors: Roberto Pérez-Torrado
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-1732-8
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022
Hardcover ISBN: 978-1-0716-1731-1Published: 06 January 2022
Softcover ISBN: 978-1-0716-1734-2Published: 07 January 2023
eBook ISBN: 978-1-0716-1732-8Published: 05 January 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 332
Number of Illustrations: 11 b/w illustrations, 53 illustrations in colour
Topics: Chemistry/Food Science, general