Abstract
Fish species from the subfamily Leuciscinae are one of the most abundant and ecologically valuable among riverine cyprinids of Central Europe. In order to support their recruitment or properly manage the restocking operations, there is a need to recognize the recruitment success which relies on the necessity of proper species discrimination among the larvae of those species caught on the spawning and/or nursery grounds. In the present study a comparative analysis of the morphological and molecular (PCR-RFLP technique with two restriction endonucleases HinfI and TaqI based on mitochondrial DNA fragment encoding cytochrome b) techniques for identification of the species status among larvae of five cyprinid species, the ide (Leuciscus idus), common dace (Leuciscus leuciscus), asp (Leuciscus aspius), roach (Rutilus rutilus) and the European chub (Squalius cephalus) was performed. It was shown that the application of morphometric features solely could create many ambiguities in species discrimination. The use of applied molecular techniques was found to be a great support for the larvae identification, regardless the developmental stage of the fish. Moreover, this molecular method may also be used for the identification of the species even if the preserved fish (e.g., in ethanol), having usually deformed and/or shrunk body caused by preservation, are about to be investigated.
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Acknowledgements
The authors would like to thank Prof. Małgorzata Jankun (UWM, Olsztyn) for carefully reading of the manuscript and giving some valuable comments. The study was supported by the project no. 0208.806 from the University of Warmia and Mazury in Olsztyn, Poland and by the project “Innovations in finfish aquaculture with special reference to reproduction” (acronym: InnovaFish), (OR14-61724-OR1400003/09/10/11).
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Kirtiklis, L., Palińska-Żarska, K., Krejszeff, S. et al. Comparison of molecular and morphometric analysis in species discrimination of larvae among five cyprinids from the subfamily Leuciscinae: A tool for sustainable conservation of riverine ichthyofauna. Biologia 71, 1177–1183 (2016). https://doi.org/10.1515/biolog-2016-0139
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DOI: https://doi.org/10.1515/biolog-2016-0139