Abstract
The goal of the work was to reveal the differences in the structure of microbial communities of Transbaikalia alkaline lakes stemming from the differences in their salinity and hydrochemical parameters. The lakes studied were Verkhnee Beloe (Buryat Republic, Russia), as well as Khilganta, Gorbunka, and Borzinskoe (Transbaikal krai, Russia) with salinity from 12.3 to 430 g/L, which differed in the mineral composition of the sediments and hydrochemical parameters. Lake sediments were found to contain 47 prokaryotic phyla (42 bacterial and 5 archaeal ones). The phyla Proteobacteria, Euryarchaeota, Bacteroides, Chloroflexi, Actinobacteria, and Firmicutes were predominant, comprising over 95% of the classified sequences. Comparative abundance of archaea increased with salinity from below 1% in Lake Verkhnee Beloe to 35% in Lake Borzinskoe. The most numerous bacterial OTUs belonged to gammaproteobacteria of the genus Halomonas (up to 15% of the number of classified sequences). The most numerous archaeal OTUs were identified at the genus level as members of the genera Halorubrum and Halohasta belonging to the family Halorubraceae, which comprises extremely halophilic Euryarchaeota.
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Ahn, A.-C., Meier-Kolthof, J.P., Overmars, L., Richter, M., Woyke, T., Sorokin, D.Y., and Muyzer, J., Genomic diversity within the haloalkaliphilic genus Thioalkalivibrio, PLoS One, 2017, vol. 12, no. 3. e0173517.
Baatar, B., Chiang, P.-W., Rogozin, D.Y., Wu, Y.-T., Tseng, C.-H., Yang, C.-Y., Chiu, H.-H., Oyuntsetseg, B., Degermendzhy, A.G., and Tang, S.-L., Bacterial communities of three saline meromictic lakes in Central Asia, PLoS One, 2016, vol. 11. e0150847.
Fadrosh, D.W., Ma, B., Gajer, P., Sengamalay, N., Ott, S., Brotman, R.M., and Ravel, J., An improved dual-indexing approach for multiplexed 16S rRNA gene sequencing on the Illumina MiSeq platform, Microbiome, 2014, vol. 2, p. 6.
Fernandez, A.B., Rasuk, M.C., Visscher, P.T., Contreras, M., Novoa, F., Poire, D.G., Patterson, M.M., Ventosa, A., and Farias, M.E., Microbial diversity in sediment ecosystems (vaporates domes, microbial mats, and crusts) of hypersaline Laguna Tebenquiche, Salar de Atacama, Chile, Front. Microbiol., 2016, vol. 7, p. 1284.
Foti, M., Ma, S., Sorokin, D.Y., Rademaker, J.L.W., Kuenen, J.G., and Muyzer, G., Genetic diversity and biogeography of haloalkaliphilic sulphur-oxidizing bacteria belonging to the genus Thioalkalivibrio, FEMS Microbiol. Ecol., 2006, vol. 56, pp. 95–101.
Foti, M., Sorokin, D.Y., Lomans, B., Mussman, M., Zakharova, E.E., Pimenov, N.V., Kuenen, J.G., and Muyzer, G., Diversity, activity and abundance of sulfatereducing bacteria in saline and hypersaline soda lakes, Appl. Environ. Microbiol., 2007, vol. 73, pp. 2093–2100.
Gorlenko, V.M., Namsaraev, B.B., Kulyrova, A.V., Zavarzina, D.G., and Zhilina, T.N., The activity of sulfatereducing bacteria in bottom sediments of soda lakes of the Southeastern Transbaikal region, Microbiology (Moscow), 1999, vol. 68, pp. 580–585.
Groth, D., Hartmann, S., Klie, S., and Selbig, J., Principal components analysis, computational toxicology, in Methods in Molecular Biology (Methods and Protocols), Reisfeld, B. and Mayeno, A., Eds., Totowa, NJ: Humana Press, 2013.
Kevbrin, V.V., Lysenko, A.M., and Zhilina, T.N., Physiology of alkaliphilic methanogen Z-7936, a new strain of Methanosalsus zhilinae isolated from Lake Magadi, Microbiology (Moscow), 1997, vol. 66, pp. 261–266.
Kozyreva, L., Egorova, D., Anan’ina, L., Plotnikova, E., Ariskina, E., Prisyazhnaya, N., Radnaeva, L., and Namsaraev, B., Belliella buryatensis sp. nov., isolated from the Lake Solenoe (Buryatia, Russia), Int. J. Syst. Evol. Microbiol., 2016, vol. 66, pp. 137–143.
Kozyreva, L.P., Egorova, D.V., Anan’ina, L.N., Plotnikova, E.A., and Namsaraev, B.B., Microbial diversity of cellulolytic community of the sandy mat from lake Zun-Torey (southern Transbaikalia), Inland Water Biol., 2014, vol. 7, pp. 134–140.
La Cono, V., Smedile, F., Bortoluzzi, G., Arcadi, E., Maimone, G., Messina, E., Borghini, M., Oliveri, E., Mazzola, S., L’Haridon, S., Toffin, L., Genovese, L., Ferrer, M., Giuliano, L., Golyshin, P.N., and Yakimov, M.M., Unveiling microbial life in new deep-sea hypersaline Lake Thetis. Part I: Prokaryotes and environmental settings, Environ. Microbiol., 2011, vol. 13, pp. 2250–2268.
Lever, M.A., Torti, A., Eickenbusch, P., Michaud, A.B., Šantl-Temkiv, T., and Jørgensen, B.B., A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types, Front. Microbiol., 2015, vol. 6, p. 476.
Merkel, A.Yu., Pimenov, N.V., Rusanov, I.I., Slobodkin, A.I., Slobodkina, G.B., Tarnovetckii, I.Yu., Frolov, E. N., Dubin, A.V., Perevalova, A.A., and Bonch-Osmolovskaya, E.A., Microbial diversity and autotrophic activity in Kamchatka hot springs, Extremophiles, 2017, vol. 21, pp. 307–317.
Mesbah, N.M., Abou-El-Ela, S.H., and Wiegel, J., Novel and unexpected prokaryotic diversity in water and sediments of the alkaline, hypersaline lakes of the Wadi An Natrun, Egypt, PLoS One, 2013, vol. 8, no. 8. e72577.
Namsaraev, B.B., Gorlenko, V.M., Namsaraev, Z.B., Dagurova, O.P., Barkhutova, D.D., Buryukhaev, S.P., and Khakhinov, V.V., Polevoi praktikum po vodnoi mikrobiologii i gidrokhimii (Field Practical Course in Aquatic Microbiology and Hydrochemistry), Ulan-Ude: Buryat. Gis. Univ., 2005.
Namsaraev, Z.B., Zaitseva, S.V., Gorlenko, V.M., Kozyreva, L.P., and Namsaraev, B.B., Microbial processes and factors controlling their activities in alkaline lakes of the Mongolian plateau, Chin. J. Oceanol. Limnol., 2015, vol. 33, pp. 1391–1401.
Nolla-Ardevol, V., Strous, M., and Tegetmeyer, H.E., Anaerobic digestion of the microalga Spirulina at extreme alkaline conditions: biogas production, metagenome, and metatranscriptome, Front. Microbiol., 2015, vol. 6, p. 597.
Quast, C., Pruesse, E., Yilmaz, P., Gerken, J., Schweer, T., Yarza, P., Peplies, J., and Glöckner, F.O., The SILVA ribosomal RNA gene database project: improved data processing and web-based tools, Nucl. Acids Res., 2013, vol. 41, pp. D590–D596.
Simachew, A., Lanzén, A., Gessesse, A., and Øvreås, L., Prokaryotic community diversity along an increasing salt gradient in a soda ash concentration pond, Microb. Ecol., 2016, vol. 71, pp. 326–338.
Sorokin, D.Y., Berben, T., Melton, E.D., Overmars, L., Vavourakis, C., and Muyzer, G., Microbial diversity and biogeochemical cycling in soda lakes, Extremophiles, 2014, vol. 18, pp. 791–809.
Sorokin, D.Y., Chernyh, N.A., and Poroshina, M.N., Desulfonatronobacter acetioxydans sp. nov.,—a first acetateoxidizing extremely salt-tolerant alkaliphilic sulfate-reducing bacterium from a hypersaline soda lake, Extremophiles, 2015, vol. 19, pp. 899–907.
Sorokin, D.Y., Gorlenko, V.M., Namsaraev, B.B., Namsaraev, Z.B., Lysenko, A.M., Eshinimaev, B.T., Khmelenina, V.N., Trotsenko, Y.A., and Kuenen, J.G., Prokaryotic communities of the north-eastern Mongolian soda lakes, Hydrobiologia, 2004, vol. 522, pp. 235–248.
Sorokin, D.Y., Kuenen, J.G., and Muyzer, G., The microbial sulfur cycle at extremely haloalkaline conditions of soda lakes, Front. Microbiol., 2011, vol. 2, pp. 1–16.
Sorokin, D.Y., Makarova, K.S., Abbas, B., Ferrer, M., Golyshin, P.N., Galinski, E.A., Ciordia, S., Mena, M.C., Merkel, A.Y., Wolf, Y.I., van Loosdrecht, M.C.M., and Koonin, E.V., Discovery of extremely halophilic, methylreducing euryarchaea provides insights into the evolutionary origin of methanogenesis, Nat. Microbiol., 2017, vol. 2, article 17081.
Sorokin, D.Y., Panteleeva, A.N., Tourova, T.P., and Muyzer, G., Haloalkaliphilic heterotrophic sulfate-reducing bacteria from soda lakes and description of Desulfonatronobacter acidivorans gen. nov., sp. nov., and Desulfobulbus alkaliphilus sp. nov., Int. J. Syst. Evol. Microbiol., 2012, vol. 62, pp. 2107–2113.
Sorokin, D.Y., Tourova, T.P., Kolganova, T.V., Sjollema, K.A., and Kuenen, J.G., Thioalkalispira microaerophila gen. nov., sp. nov., a novel lithoautotrophic, sulfur-oxidizing bacterium from a soda lake, Int. J. Syst. Evol. Microbiol., 2002, vol. 52, pp. 2175–2182.
Tsyrenova, D.D., Bryanskaya, A.V., Namsaraev, Z.B., and Akimov, V.N., Taxonomic and ecological characterization of cyanobacteria from some brackish and saline lakes of Southern Transbaikal Region, Microbiology (Moscow), 2011, vol. 80, pp. 216–227.
Tsyrenova, D.D., Kozyreva, L.P., Namsaraev, B.B., Bryanskaya, A.V., and Namsaraev, Z.B., Structure and formation properties of the haloalkaliphilic community of Lake Khilganta, Microbiology (Moscow), 2011, vol. 80, pp. 237–243.
Vasil’ev, E.K., Kachestvennyi rengenofaznyi analiz (Qulitative X-ray Phase Analysis), Novosibirsk: Nauka, 1986.
Vavourakis, C.D., Ghai, R., Rodriguez-Valera, F., Sorokin, D.Y., Tringe, S.G., Hugenholtz, P., and Muyzer, G., Metagenomic insights into the uncultured diversity and physiology of microbes in four hypersaline soda lake brines, Front. Microbiol., 2016, vol. 7, p. 211.
Xiong, J., Liu, Y., Lin, X., Zhang, H, Zeng, J., Hou, J., Yang, Y., Yao, T., Knight, R., and Chu, H. Geographic distance and pH drive bacterial distribution in alkaline lake sediments across Tibetan Plateau, Environ. Microbiol., 2012, vol. 14, pp. 2457–2466.
Zaitseva, S.V., Abidueva, E.Yu., Buryukhaev, S.P., and Namsaraev, B.B., Factors controlling the activity of the microbial community of the alkaline Lake Beloe (Transbaikal region), Microbiology (Moscow), 2012, vol. 81, pp. 468–476.
Zakharyuk, A., Kozyreva, L., Ariskina, E., Troshina, O., Kopitsyn, D., and Shcherbakova, V., Alkaliphilus namsaraevii sp. nov., an alkaliphilic iron-and sulfur-reducing bacterium isolated from a steppe soda lake, Int. J. Syst. Evol. Microbiol., 2017, vol. 67, pp. 1990–1995.
Zakharyuk, A.G., Kozyreva, L.P., Khijniak, T.V., Namsaraev, B.B., and Shcherbakova, V.A., Desulfonatronum zhilinae sp. nov., a novel haloalkaliphilic sulfate-reducing bacterium from soda Lake Alginskoe, Trans-Baikal Region, Russia, Extremophiles, 2015, vol.19, pp. 673–680.
Zhong, Z.-P., Liu, Y., Miao, L.-L., Wang, F., Chu, L.-M., Wang, J.-L., and Liu, Z.-P., Prokaryotic community structure driven by salinity and ionic concentrations in plateau lakes of the Tibetan Plateau, Appl. Environ. Microbiol., 2016, vol. 82, pp. 1846–1858.
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Original Russian Text © S.V. Zaitseva, E.Yu. Abidueva, A.A. Radnagurueva, S.M. Bazarov, S.P. Buryukhaev, 2018, published in Mikrobiologiya, 2018, Vol. 87, No. 4.
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Zaitseva, S.V., Abidueva, E.Y., Radnagurueva, A.A. et al. Structure of Microbial Communities of the Sediments of Alkaline Transbaikalia Lakes with Different Salinity. Microbiology 87, 559–568 (2018). https://doi.org/10.1134/S0026261718040185
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DOI: https://doi.org/10.1134/S0026261718040185