Abstract
While many parasites develop within host cells to avoid antibody responses and to utilize host cytoplasmic resources, elaborate egress processes have evolved to minimize the time between escaping and invading the next cell. In human erythrocytes, malaria parasites perforate their enclosing erythrocyte membrane shortly before egress. Here, we show that these pores clearly function as an entry pathway into infected erythrocytes for compounds that inhibit parasite egress. The natural glycosaminoglycan heparin surprisingly inhibited malaria parasite egress, trapping merozoites within infected erythrocytes. Labeled heparin neither bound to nor translocated through the intact erythrocyte membrane during parasite development, but fluxed into erythrocytes at the last minute of the parasite lifecycle. This short encounter was sufficient to significantly inhibit parasite egress and dispersion. Heparin blocks egress by interacting with both the surface of intra-erythrocytic merozoites and the inner aspect of erythrocyte membranes, preventing the rupture of infected erythrocytes but not parasitophorous vacuoles, and independently interfering with merozoite disaggregation. Since this action of heparin recapitulates that of neutralizing antibodies, membrane perforation presents a brief opportunity for a new strategy to inhibit parasite egress and replication.
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Introduction
To evade immune detection, intracellular apicomplexan parasites replicate within several layers of membranes1,2. However, this replication is only advantageous if parasite progeny can emerge successfully from host cells after breaking both the parasitophorous vacuolar membrane and the erythrocyte plasma membrane to invade new cells. The mechanics of malaria parasite egress from erythrocytes are still unresolved despite the growing knowledge of regulated enzymatic and signaling events involved in the egress mechanism3. Morphological transformations of the two parasite-enclosing membranes prior to parasite release were visualized in a limited number of studies4,5,6,7 in which it was suggested that vacuolar membrane rupture occurs prior to or simultaneous with the opening of the erythrocyte membrane. The functional proteins for vacuolar membrane rupture are not currently known. However, the erythrocyte cysteine protease calpain8 and the parasite surface protein MSP19 have each been implicated in degradation of the erythrocyte cytoskeleton, a crucial step in parasite egress preparation leading to the opening of the host cell membrane. One of the final changes in the infected erythrocyte prior to egress is perforation of the erythrocyte membrane10. Perforation of the host cell membrane by Apicomplexan parasites was first demonstrated in Toxoplasma-infected cells11 and later was shown in Plasmodium-infected erythrocytes for both sexual and asexual forms12,13,14. Toxoplasma pore-forming protein 1 perforates both vacuolar and host plasma membranes while perforin-like protein 2 of P. falciparum is involved in perforation of the erythrocyte but not vacuolar membrane, and only in sexual forms of the parasite. Perforin-like protein 1 of P. falciparum was suggested to perforate both the parasitophorous vacuolar membrane and the erythrocyte membrane during the asexual cycle15, but a recent study demonstrated it is dispensable for blood-stage parasite growth16.
While investigating the inhibition of P. falciparum invasion of erythrocytes by heparin and other highly-charged sulfated glycosaminoglycans (GAGs)17,18,19,20, we noticed entry of these inhibitory compounds into infected cells through the membrane of erythrocytes. Entry could only occur at the very end of the replicative cycle, to inhibit parasite egress. This was a surprising observation, because in analogy to antiviral mechanisms21, it is postulated that heparin inhibits invasion in vitro by binding to merozoite surface proteins just prior to invasion, thus blocking subsequent merozoite binding to the erythrocyte surface17 (there is a clear non-specific loss of merozoite adhesion)22. Thus, perforation of the erythrocyte membrane just before parasite egress provides a novel entry pathway of high-molecular-weight hydrophilic compounds able to prevent parasite dissemination. Consistent with reports of delayed schizont rupture in the presence of several heparin-like compounds19,23, heparin exploits a natural stage in the parasite’s intra-erythrocytic developmental cycle (IDC), i.e. erythrocyte membrane perforation preceding membrane rupture during the last minute of the replicative cycle12. Our findings are the first indication of a technological application for the molecular-perforation stage of infected erythrocytes prior to egress.
Results
Heparin inhibits parasite egress in vitro
To assess heparin inhibition of parasite egress in vitro, we exposed P. falciparum schizonts to 25–100 µg/ml of heparin for 60–90 minutes at 37 °C. Using a quantitative egress assay24, we found that heparin had a reproducible, dose-dependent inhibitory effect on parasite egress from infected erythrocytes (Fig. 1A). Exposure to 100 µg/ml of heparin, which is known to strongly inhibit merozoite invasion of erythrocytes17, not only inhibited the egress of laboratory strain NF54 parasites to less than half of the control level but also inhibited the egress of two artemisinin-resistant P. falciparum clinical isolates, CP803 and RF967, up to 45% and 35%, respectively (Fig. 1B). These data suggest that heparin exerts a strain-transcendent inhibitory effect on parasite egress.
Live-cell microscopy of heparin-treated cultures revealed accumulation of morphologically-distorted infected cells composed of clustered merozoites mostly trapped within erythrocytes (Fig. 1C). Quantitative analysis of biological outcomes of the egress assay, i.e. counting the number of parasite egress sites and merozoite clusters in heparin-treated and control cultures, shows that (i) the sum of egress sites and clusters in heparin-treated cultures (98.0 ± 12.4% of the control, mean ± SD) were similar to those of the control (p = 0.83, two-tailed one sample t-test, H0 = 100%, n = 3), suggesting that both cultures normally progressed to the end of the cycle; and (ii) inhibition of egress in heparin-treated cultures is explained by significantly increased cluster formation (Fig. 1D). Together, our data suggest that heparin does not affect the last two hours of the parasite IDC, but interferes with the egress of mature merozoites.
Mechanism of parasite egress inhibition by heparin
The final mechanical steps of egress include rupture of the parasitophorous vacuole, parasite dissociation within the schizont, and perforation and rupture of the erythrocyte membrane5,10,12. The scattered discharge of individual merozoites from the erythrocyte then completes the IDC5. To investigate the mechanism of egress inhibition by heparin, we first used time-lapse light microscopy to record parasite egress in medium containing 100 µg/ml of heparin and frequently observed blocked egresses (see Supplementary Movie 1 and Fig. 2A showing selected frames from this movie), leading to the formation of semi-dissociated merozoites, or merozoite clusters, retained within erythrocytes. The observed outcome mimics the static images presented in Fig. 1C. These data suggest that heparin interferes with the rupture of merozoite-enclosing membranes and likely affects merozoite dispersion prior to egress.
To further assess heparin’s effect on merozoite dispersion, we analyzed egress sites that are preserved in the chambers for microscopy and identified one prominent feature: egressed or partially-egressed merozoites were often in aggregates of varied sizes, indicating variable degrees of merozoite dispersion (Fig. 2B). 56.5 ± 11.4% (mean ± SD) of egress sites in cultures treated with 100 µg/ml of heparin (four independent experiments, n = 130) contained merozoite clusters of varied sizes in comparison to 17.7 ± 8.6% of sites in control cultures (three independent experiments, n = 97, p = 0.004, two-tailed t-test). The heparin-induced merozoite dispersion defect may account for part of heparin’s reported inhibition of parasite invasion, as merozoites that fail to detach from their siblings are expected to be invasion-incompetent.
To investigate the integrity of the parasitophorous vacuolar membrane (PVM) that encloses divided parasites, we generated an NF54 strain with a fluorescent mNeonGreen tag on the C-terminus of the endogenous EXP2 protein, a resident of the PVM25 (Fig. 3A, upper panel). EXP2-mNeonGreen infected cells were treated with 100 μg/ml heparin and inspected for PVM integrity using fluorescence microscopy. A broken PVM was observed in 85% of clustered merozoites (n = 60) in heparin-treated cultures (Fig. 3A, lower panel), in contrast to 5% of broken PVM in the apparently normal schizonts of control cultures (n = 64).
In a separate experiment to test the integrity of the PVM, we incubated infected cells with Lucifer Yellow CH (LY), a fluorescent marker for the PV space and merozoites (Fig. 3B, upper panel)26,27 and inspected dye distribution within infected cells in heparin-treated cultures. LY was not found around parasite clusters within infected cells (Fig. 3B, lower panel) but rather only in merozoites, presumably because LY leaked out of the PV upon rupture of the PVM. Together, these data suggest that most heparin-induced merozoite clusters harbored broken PVMs, and therefore that heparin does not interfere with PV disintegration at the end of the IDC.
To investigate whether heparin inhibits erythrocyte membrane rupture, we used Alexa Fluor 488-labeled phalloidin, which binds erythrocyte cytoskeletal F-actin28. Fluorescent phalloidin enters infected erythrocytes during parasite-induced perforation of the erythrocyte membrane, which precedes natural parasite egress12,14, and labels the inner erythrocyte membrane. When we exposed wild-type NF54 schizonts to Alexa Fluor 488-phalloidin and 100 µg/ml of heparin (three independent experiments) and analyzed them by fluorescence microscopy, we found that 95.6 ± 4.82% of inspected clusters (mean ± SD, n = 3, total number of inspected cells = 181) were surrounded with erythrocyte membrane permeable for fluorescent phalloidin, while only 0.55 ± 0.95% (mean ± SD, three experiments) of 181 morphologically normal schizonts in the same cultures were fluorescent (Fig. 3C). These data suggest that (i) heparin does not interfere with parasite-induced erythrocyte membrane perforation, (ii) clusters are enclosed in the erythrocyte membrane, and (iii) heparin was able to enter infected cells before parasite egress. Next, we evaluated macroscopic erythrocyte membrane integrity in 119 randomly-selected, phalloidin-labeled clusters in heparin-exposed cultures (cumulative data of two independent experiments). About 75% of the phalloidin-labeled erythrocytes contained merozoite clusters, enclosed in apparently intact erythrocyte membranes (Fig. 3D, upper panel, and Fig. 3E), while the rest of infected cells had macroscopic defects in the erythrocyte membrane (Fig. 3D, lower panel, and Fig. 3E). These data confirm that heparin interferes with erythrocyte membrane rupture or erythrocyte membrane shedding that could proceed once the membrane is broken.
Thus, heparin inhibits parasite egress and reinvasion by both blocking erythrocyte membrane rupture and impairing parasite dispersion at the end of the IDC.
The mode of heparin interaction with infected erythrocytes and heparin targets
To investigate heparin’s interaction with infected erythrocytes and its molecular targets, we used a FITC-heparin conjugate and live-cell fluorescence microscopy. FITC-heparin was not found on the surface of uninfected or infected erythrocytes during 120 minutes of incubation (Fig. 4A,B) and did not accumulate within schizonts, which can acquire other labels5,27. Thus, intact membranes of uninfected and infected erythrocytes neither bind nor translocate these highly-charged polymerized heparin molecules, so the mechanism of egress inhibition is likely related to the very end of the cycle. To further explore the parasite-heparin interaction, we recorded parasite egress in the presence of FITC-heparin, and found that FITC-heparin conjugate enters pre-egressing schizonts just prior to erythrocyte membrane rupture (Fig. 4C shows selected frames from Supplementary Movie 2). This timing of FITC-heparin entry matches the previously-reported time for parasite-induced perforation of the erythrocyte membrane that precedes egress12. The pore size was apparently large enough for FITC-heparin influx, as was shown in experiments with the cysteine protease inhibitor E-64, which blocks parasite egress from erythrocytes but does not affect erythrocyte membrane perforation10. All inspected E-64-induced merozoite clusters contained FITC-heparin within erythrocytes (Fig. 4D, upper panel; compare with Fig. 4D, lower panel showing an immature schizont in the same culture with intact erythrocyte membrane not labeled with FITC-heparin). However, when a specific inhibitor of PfPKG, Compound 2 (C2)29,30, is used to entirely block parasite egress upstream of vacuolar and erythrocyte membrane perforation and rupture, morphologically-abnormal infected cells appear (63% of all infected cells, n = 200), which are different from the heparin-induced clusters and are not permeable for FITC-heparin (n = 60) (Fig. 4E). Thus, by investigating various stages of egress, both before erythrocyte membrane perforation (C2 treatment) and after this step (E-64 treatment), we have strengthened our conclusion that heparin can diffuse through parasite-modified erythrocyte membranes prior to egress.
Two FITC-heparin targets were observed: the inner erythrocyte membrane just before egress or in the stalled parasite clusters, and the merozoite surface in clusters or egressed parasites (Fig. 5A,B). By observing the membranes of infected erythrocyte ghosts, which had extruded their PVM-enclosed parasites (we confirmed the presence of PVM around the extruded schizonts using EXP2-mNeonGreen-labeled, NF54-infected cells, Fig. 5C), we concluded that the FITC-heparin signal at the inner erythrocyte membrane (Fig. 5D) originated from a parasite source for two reasons: (i) uninfected erythrocyte ghosts do not bind FITC-heparin (n = 52, also see Fig. 5E, white arrowhead); and (ii) the intensity of erythrocyte membrane fluorescence is directly correlated (r2 = 0.98) with the multiplicity of erythrocyte infection (Fig. 5F, n = 80). These observations suggest that heparin targets are parasite-derived proteins, rather than infection-modified erythrocyte cytoskeletal proteins.
While the inner erythrocyte membrane showed a FITC-heparin fluorescence signal that was stable for several hours, merozoites showed a transient fluorescence signal that disappeared within 15 minutes after egress (Fig. 6A). Quantitative image analysis of individual merozoites released from the same schizont in time-lapse recordings (every 60 seconds) showed that fluorescence loss from the merozoite surface had a stochastic onset (Fig. 6B) and fast progression (Fig. 6C). Initiation of fluorescence loss was spread over a period of 205.2 ± 48.1 seconds (mean ± SEM) and characterized by an exponential with a time constant ± 95% confidence interval of 147.2 ± 81.5 seconds (Fig. 6B). Single-exponential decay equations fit to the fluorescence loss of parasites from the point of loss initiation (the end of the “shoulder” of fluorescence loss) had a time constant of 175.2 ± 72.6 seconds (mean ± SEM, four experiments with 3, 5, 7, and 5 individually-measured parasites in each experiment).
The high fluorescence intensity on merozoite surfaces indicates an abundance of FITC-heparin target. The transient nature of this signal suggests that heparin may bind proteins that are shed during merozoite invasion by the Ca2+-dependent subtilisin-type protease PfSUB2 and rhomboid proteases PfROM1 and PfROM431,32. To discriminate between these, we investigated the Ca2+-dependence of heparin shedding in vitro. The drop in merozoite surface fluorescence was abolished when merozoites egressed into Ca2+-free medium (Fig. 6D), suggesting that the shedding of MSP1, the only known target of PfSUB2 on the merozoite surface that can bind heparin33,34,35,36, is causing the fluorescence loss. The enhanced stability of merozoite surface fluorescence in Ca2+-free medium enabled us to evaluate the photobleaching kinetics of FITC-heparin as a function of multiple scans. Quantitative image analysis revealed a slight decline in fluorescence over time (Fig. 6E), which can be ascribed to photobleaching. Thus, photobleaching was induced and measured by imaging the merozoites with frequent frame rates, and then quantified as a function of frame number (mean ± SEM frame constant, 35.7 ± 11.16 frames, n = 18 merozoites from two experiments). Knowing the rate of observed fluorescence decay in Ca2+-containing medium (one frame per minute), we calculated a time constant of decay due to photobleaching of 2140 seconds (~36 minutes), 12-fold longer than that observed above. This result excludes photobleaching of FITC as a significant factor in the disappearance of fluorescence from the merozoite surface in Ca2+-containing medium (Fig. 6C). The observed asynchronous nature of FITC-heparin shedding likely reflects a stochastic interaction between merozoite and putative shedding-inducing stimuli, such as receptors on erythrocyte membrane fragments at egress sites. After initiation, shedding proceeded at the same rate in most merozoites, supporting the possibility that it is an enzymatic reaction. Together, these data suggest that MSP1 is the most likely merozoite surface protein to bind heparin in our experiments.
A third heparin target was detected as the sudden appearance of a strong punctate fluorescence signal at the merozoite apical prominence (visualized by DIC microscopy) following shedding of heparin from the merozoite surface, i.e. not immediately after egress (Fig. 7A, Supplementary Movie 3), which then persisted for a prolonged period. The sequential nature of the shedding of heparin-binding merozoite surface proteins and appearance of apical fluorescence signal seem to represent two independent steps of the invasion process. Blocking the shedding of heparin from the merozoite surface in Ca2+-free medium did not affect the timely appearance of apical fluorescence signal on merozoites (Fig. 7B). The extrusion of apical material somewhat mimics the events of natural merozoite invasion in vitro 37,38 and may be involved in the reported inhibition of parasite invasion by heparin17, but not in Ca2+-free medium39,40.
Discussion
Our data show that the highly-charged natural polymer heparin inhibits P. falciparum egress in vitro, exploiting a previously-unrecognized mode of interaction with infected erythrocytes from within, entering cells through parasite-derived pores in the erythrocyte membrane prior to parasite egress. The following heparin-cell interactions show the topology of proteins that are critically involved in the egress mechanism: surface proteins of merozoites and the inner aspect of erythrocyte membranes. The mechanics of egress inhibition includes inhibition of erythrocyte membrane rupture and prevention of merozoite dispersion. The fast kinetics of heparin binding to its targets and high intensity of FITC-heparin signal at the binding sites suggest that heparin has a high binding affinity for abundant parasite proteins. One of them is likely MSP1, a major merozoite surface protein that was recently implicated in parasite egress9. It was suggested that MSP1 interacts with spectrin, a component of the erythrocyte cytoskeleton, to disrupt the erythrocyte membrane and release merozoites. MSP1 is the most likely heparin target on the merozoite surface because it is a major merozoite surface protein and the only known heparin-binding protein to be shed from the merozoite surface by the Ca2+-dependent protease PfSUB2 after parasite egress34,35,41. Heparin-MSP1 binding may also explain poor merozoite dispersion. The heparin-induced merozoite clusters we observed appear to be the same as those induced by human antibodies specific for merozoite surface proteins42. The binding of polyvalent molecules such as heparin and antibodies to the merozoite surface may impair merozoite dispersion by effectively creating an adhesive between adjacent merozoites, preventing the formation of singular merozoites that are responsible for initiating a new round of erythrocyte invasion and parasite replication. Heparin binding to the inner erythrocyte membrane, which we suggest prevents erythrocyte membrane rupture and thus egress, could involve one or more parasite proteins known both to interact with components of the erythrocyte membrane and to bind heparin36. These include PfEMP2 (MESA), which interacts with the erythrocyte cytoskeletal protein 4.143,44,45,46, and CLAG 3.1–3.2, which are proposed components of a nutrient channel in infected erythrocytes47.
Besides playing a role in egress, MSP1 is thought to mediate the merozoite’s interaction with the erythrocyte surface upon invasion17,48,49,50, after which time MSP1 and several other surface proteins are shed from the merozoite as it enters an erythrocyte41. This Ca2+-dependent protein shedding51, assayed as MSP-1 processing in vitro using purified merozoites, has apparent first-order kinetics with an MSP-1 processing half-life of approximately 20 minutes52. Here we show that FITC-heparin is also shed from live egressed merozoites in a similarly Ca2+-dependent manner. By not interfering with surface protein shedding, heparin might not inhibit merozoite penetration into erythrocytes. However, specific merozoite-erythrocyte interactions that precede invasion may be negatively affected by heparin. Indeed, several studies suggest that stages prior to merozoite penetration are blocked in the presence of heparin17,22.
The sudden appearance of a third heparin-binding site on the apical prominence of merozoites late after egress was independent of Ca2+-dependent protease activation, and seemed to occur with or without shedding of the second binding partner from parasite surfaces. Since the merozoite is fully formed at this third stage of apical heparin binding, the appearance of this late membrane-bound heparin target can occur either by merozoite extrusion or enzymatic modification of pre-existing surface proteins. Based on electron microscopy images of invading merozoites extruding rhoptry material into erythrocytes37,38 and observations that rhoptry proteins are well represented among the parasite’s heparin-binding proteins36,53, we suggest that this heparin target is a component of the extruded rhoptry material.
Our data suggest two heparin targets on the merozoite surface because merozoites could initiate a pseudo-invasion program under our experimental invasion-free conditions. In 1992, Trager et al.54 showed that extra-erythrocytic parasite development might occur in vitro in medium supplemented with erythrocyte homogenate. Here we show that regular culture medium supports early developmental steps. The dynamic interaction of heparin with merozoites that we observed using live-cell microscopy might explain a controversy originating from previous publications that used static-cell imaging. One paper described heparin binding to the apical region of merozoites53, while another showed heparin binding to the entire merozoite surface55. In addition, a developmental sequence of protein exposures regulated by a timed series of protein release from parasite secretory organelles is highly reminiscent of echinoderm zygotic development after fertilization56.
Conceptually, this work demonstrates that egress mechanisms can be targeted with large hydrophilic drugs, opening new avenues for antimalarial drug development that exploit the parasite’s own induction of a short-lasting stage-specific permeability to circumvent the anticipated membrane barrier to drug entry57. Heparin’s interaction with merozoite surface proteins and infection-modified erythrocyte membranes could be centrally important to understanding the critical mediators of parasite egress, parasite dispersion, and dissemination in general. Heparin’s fast entry is in part due to the very small volume between the merozoites and the plasma membrane just before egress, allowing diffusion to rapidly fill this space. Its action inside infected erythrocytes demonstrates the feasibility of employing proteins, peptides, or other natural polymers targeting the parasite egress mechanism to prevent parasite dissemination. Indeed, heparin recapitulates some of the effects exerted by immune antibodies42, and does so in several P. falciparum strains, thus lacking the specificity of antibodies. Polypeptides complementary to the functionally-conserved sequences of parasite proteins involved in parasite egress and invasion may be worth investigating to develop antimalarials that interact with intracellular parasites, including drug-resistant strains.
Methods
P. falciparum strains and human erythrocytes
We used three strains of P. falciparum: NF54, a long-term-adapted laboratory reference strain (ATCC, Manassas, VA); and CP803 and RF967, two short-term-adapted, artemisinin-resistant clinical isolates from Cambodian patients with malaria58. The clinical isolates were collected on a protocol (ClinicalTrials.gov identifier no. NCT00341003) approved by the Cambodian National Ethics Committee for Health Research and NIAID Institutional Review Board (IRB), in which patients gave written informed consent, and were maintained in culture in our laboratories.
Generation of NF54 EXP2-mNeonGreen parasites
Integration of an mNeonGreen fusion at the endogenous EXP2 C-terminus was accomplished with CRISPR/Cas9 editing. A guide RNA target downstream of the exp2 stop codon was chosen and the gRNA seed sequence was ordered as sense and antisense oligo pairs (P1/P2), annealed and inserted into the plasmid pAIO59 at BtgZI using an In-Fusion cloning kit (Clontech), resulting in the plasmid pAIO-EXP2-CT-gRNA. The vector pPM2GT60 was modified to replace the hDHFR selection cassette with a yDHODH selection cassette amplified from the plasmid pUF-161 using primers P3/P4 and inserted between BglII and SalI sites, resulting in the vector pyPM2GT. A 5′ homology donor template (up to but not including the exp2 stop codon) and a 3′ homology donor template (beginning 156 bp downstream of the exp2 stop codon just after the gRNA seed sequence) were PCR amplified from P. falciparum NF54 genomic DNA (primers P5/6 and P7/8, respectively), assembled in a second PCR reaction using primers P7/P6, and inserted between XhoI and AvrII sites in pyPM2GT. The mNeonGreen coding sequence62 was amplified with primers P9/10 (adding a flexible linker between EXP2 and mNeonGreen) and inserted between AvrII and EagI sites, resulting in the plasmid pyPM2GT-EXP2-mNeonGreen. This vector was linearized at the AflII site between the 3′and 5′ donor sequences and co-transfected with pAIO-EXP2-CT-gRNA into P. falciparum NF54attB parasites63. Selection with 2 µM DSM-1 was applied 24 hours post transfection. After returning from selection, integration at the intended site was confirmed by PCR using primers P11/P12. A clonal line containing the EXP2-mNeonGreen fusion was obtained by limiting dilution.
List of used primers for generation of NF54-EXP2-mNeonGreen parasites.
P1 TAAGTATATAATATTatattatgtacagtatctgaGTTTTAGAGCTAGAA
P2 TTCTAGCTCTAAAACtcagatactgtacataatatAATATTATATACTTA
P3 GGGAGACCGGCAGATCTTATAAGGAAATTCCC
P4 ATGCCTGCAGGTCGACTCTAGAGGATCCCCGG
P5 GTTTGATTATTTTATTTATGTACTCTCCTTATGACTTAAGCCTTGAGAGAAATATGGGAT
P6 CTGCACCTGGCCTAGGTTCTTTATTTTCATCTTTTTTTTCATTTTTAAATAAATCTCCAC
P7 CACTATAGAACTCGAGGGAGAAACAATCTTTTATATAAAATGTACAGAGTTTGAAAG
P8 ATCCCATATTTCTCTCAAGGCTTAAGTCATAAGGAGAGTACATAAATAAAATAATCAAAC
P9 GATGAAAATAAAGAACCTAGGGGAAGTGGAGGAGTGAGCAAGGGCGAGGAGGATAAC
P10 TAACTCGACGCGGCCGTCACTTGTACAGCTCGTCCATGCCCATC
P11 GCAACAAGTGCCTTAACCACCG
P12 GTAAGTCTTCTTCGACCTGCAC
*gRNA seed sequences are showing in lower case.
We maintained all parasite cultures in human erythrocytes isolated from the blood of healthy donors (who had consented to participate in the NIH IRB-approved Research Donor Program in Bethesda, MD; all samples were anonymized) in culture medium: RPMI 1640 medium supplemented with 25 mM HEPES, 0.1 mM hypoxanthine, 25 µg/ml gentamicin, 0.5% Albumax II (all from Gibco, Waltham, MA), and 4.5 mg/ml glucose (Sigma, St. Louis, MO). Cultures were maintained at low parasitemia and 5% hematocrit.
Heparin
We used heparin (H3149, Sigma) at concentrations of 25–100 µg/ml in culture medium (~20 U/ml of physiological activity) or fluorescently-labelled heparin (FITC-heparin, H7482, Life Technologies, Waltham, MA) at concentrations of 1–20 µg/ml in culture medium.
Inhibitors
Compound 2 (4-[7-[(dimethylamino)methyl]-2-(4-fluorphenyl) imidazo[1,2-a] pyridine-3-yl]pyrimidin-2-amine; MRT00072329) was provided by Dr. Simon Osborne, Medical Research Council Technology (MRCT), United Kingdom and E-64, a cysteine protease inhibitor, was purchased from Sigma (E3132).
Parasite egress assays
We performed live-cell recording as described24 using an LSM 510 laser-scanning microscope (Carl Zeiss AG, Oberkochen, Germany) with a 63 × 1.4 NA oil objective and 488-nm laser with low light intensity to minimize cell photodamage. To observe parasite egress, we isolated schizonts from culture using 65% Percoll64,65, adjusted them to 0.1–0.2% hematocrit in media of different compositions at 37 °C, and placed them into special environmental chambers for microscopy (HybriWell HBW20, Grace Bio-Labs, Inc., Bend, OR). These chambers preserve cell viability for several hours, as well as egress sites, i.e., places where schizonts ruptured and released merozoites at the end of their IDC24. To quantify the effect of heparin on egress, we kept chambers at 37 °C for 60–90 minutes to accumulate egress sites, and then cooled them at 15 °C for 30 minutes to stop egress. We quantified egress as the fraction of schizonts releasing merozoites.
Quantitative image analysis
We manually selected individual FITC-heparin-labeled merozoite regions of interest (ROIs, 2 μm × 2 μm) in time-lapse recordings to measure loss of cell fluorescence, excluding those that were tightly packed (<2 μm) or moving out of focus. We tracked relative merozoite positions using a cross-correlation search strategy, matching merozoites at each time point to a static reference template created by averaging the positions of four merozoites. This corrected for stage drift through time and for small lateral cell movements. We then measured the average fluorescence within each ROI, and time-centered this value at the initiation of fluorescence loss. We manually identified initiation points by detecting the end of the “shoulder” of fluorescence loss, and the beginning of the period resembling an exponential decay. We then fit single-exponential decay equations to the 500-second period beginning at the initiation point for each merozoite, and averaged the resulting time constants. We measured photobleaching by fitting exponential decay functions on a frame-wise basis during the photobleaching period at the end of experiments with blocked heparin complex shedding. We then multiplied the resulting frame constants by the time per frame to obtain a photobleaching time course.
To quantify the FITC-Heparin signal from the membrane of ghosts in single- and multiple-infected RBCs, one confocal image was taken at the equator of the RBC with a pinhole of 1 Airy Unit. All imaging parameters were kept constant after adjusting them to prevent detector saturation. RBC membrane fluorescence was then thresholded using the Otsu method66 to exclude background fluorescence. The mean pixel intensity of the thresholded image was taken as a data point.
All methods using in this research were carried out in accordance with relevant guidelines and regulations.
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Acknowledgements
We thank Kamran Melikov and Paul Blank for fruitful discussions. This study was funded by the Intramural Research Programs of the Eunice Kennedy Shriver National Institute of Child Health and Human Development, and the National Institute of Allergy and Infectious Diseases, National Institutes of Health. Josh R. Beck acknowledges funding from the National Institutes of Health (NIH K99 HL133453). DEG funded by NIH grant AI-112508.
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S.G., J.B., and J.Z. conceived and designed experiments. S.G., B.B., J.B., and M.G. performed experiments. All authors analyzed data and wrote the manuscript.
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Glushakova, S., Busse, B.L., Garten, M. et al. Exploitation of a newly-identified entry pathway into the malaria parasite-infected erythrocyte to inhibit parasite egress. Sci Rep 7, 12250 (2017). https://doi.org/10.1038/s41598-017-12258-x
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DOI: https://doi.org/10.1038/s41598-017-12258-x
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