Abstract
Despite recent insights into prostate cancer (PCa)-associated genetic changes, full understanding of prostate tumorigenesis remains elusive owing to complexity of interactions among various cell types and soluble factors present in prostate tissue. We found the upregulation of nuclear factor of activated T cells c1 (NFATc1) in human PCa and cultured PCa cells, but not in normal prostates and non-tumorigenic prostate cells. To understand the role of NFATc1 in prostate tumorigenesis in situ, we temporally and spatially controlled the activation of NFATc1 in mouse prostate and showed that such activation resulted in prostatic adenocarcinoma with features similar to those seen in human PCa. Our results indicate that the activation of a single transcription factor, NFATc1 in prostatic luminal epithelium to PCa can affect expression of diverse factors in both cells harboring the genetic changes and in neighboring cells through microenvironmental alterations. In addition to the activation of oncogenes c-MYC and STAT3 in tumor cells, a number of cytokines and growth factors, such as IL1β, IL6 and SPP1 (osteopontin, a key biomarker for PCa), were upregulated in NFATc1-induced PCa, establishing a tumorigenic microenvironment involving both NFATc1 positive and negative cells for prostate tumorigenesis. To further characterize interactions between genes involved in prostate tumorigenesis, we generated mice with both NFATc1 activation and Pten inactivation in prostate. We showed that NFATc1 activation led to acceleration of Pten null-driven prostate tumorigenesis by overcoming the PTEN loss-induced cellular senescence through inhibition of p21 activation. This study provides direct in vivo evidence of an oncogenic role of NFATc1 in prostate tumorigenesis and reveals multiple functions of NFATc1 in activating oncogenes, in inducing proinflammatory cytokines, in oncogene addiction, and in overcoming cellular senescence, which suggests calcineurin-NFAT signaling as a potential target in preventing PCa.
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Introduction
Recent discoveries in prostate cancer (PCa) research have highlighted a number of key genetic alterations in driving prostate tumorigenesis. Despite these advances, the progression from the initial genetic changes to clinically significant PCa is still not fully understood.1 Furthermore, the interactions of various genes involved in pathogenesis of PCa needs to be further investigated.
The NFAT (nuclear factor of activated T cells) family of transcription factors are important for many diverse cellular processes including T-cell activation, cardiac valve development and osmotic stress response.2, 3 The NFAT family includes four NFATc proteins (c1–c4) and NFAT5. The NFATc proteins reside in the cytoplasm in quiescent cells. The Ca2+-dependent serine/threonine phosphatase calcineurin, when activated by an increase in intracellular Ca2+, dephosphorylates the NFATc proteins and exposes their concealed nuclear localization signals, causing them to translocate from cytoplasm to the nucleus.2, 3 Once in the nucleus, NFATc proteins complex with cell-type specific cofactors to control the transcription of target genes. Phosphorylation of NFATc by GSK3β and other kinases promotes nuclear export of these proteins.
NFATc1 activation was shown to induce transformation and colony formation in 3T3-L1 preadipocytes.4 Elevated or ectopic NFATc1 activation has also been observed in multiple types of human tumors.3, 5 NFAT proteins have also been found to regulate proliferation and growth of multiple human tumor cells, including PCa cells.3, 6, 7 NFATc1 has been shown to regulate prostate-specific membrane antigen and genes involved in osteoclastogenesis induced by PCa cells.8, 9, 10 Besides growth and proliferation, components of the calcineurin-NFAT signaling pathway, including NFATc1, have also been linked to cell migration, tumor invasion and metastasis in human cancers.3, 11, 12, 13, 14, 15 NFATc1 effects are not always pro-growth and some NFATc genes may act as tumor suppressors.3, 16 NFATc1 activation can lead to cell loss and fibrosis in some contexts.3, 17 Thus, biological consequences of NFAT activation in different tissues may be very different and the molecular mechanism by which calcineurin-NFAT affects PCa in situ is hard to predict and needs to be directly studied.
In this study, we generated a murine model where NFATc1 activation can be induced in prostate epithelium. The activation of NFATc1 results in prostatic intraepithelial neoplasia (PIN), which progresses to prostate adenocarcinoma. We further demonstrated that NFATc1 activation establishes a promitogenic microenvironment with upregulation of proinflammatory cytokines and growth factors. We have also shown that NFATc1 and the PTEN-AKT pathway act synergistically in promoting PCa, as NFATc1 activation overcomes the PTEN loss-induced cellular senescence. This study provides direct in vivo evidence of an oncogenic role of NFAT in PCa and offers insights into multi-faceted progression from a defined transcriptional change in prostatic epithelia to prostate tumorigenesis involving both cell autonomous changes in oncogenic protein expression and the effects of secreted factors in the microenvironment.
Results
NFATc1 expression is detected in human PCa specimens and PCa cells but is absent in non-neoplastic human prostates and non-tumorigenic prostatic cells.
NFATc1 expression has been previously reported in human PCa specimens.18, 19, 20 Using human normal prostate and PCa specimens from Biomax (Rockville, MD, USA) and from archived patient specimens, we found NFATc1+ cells in the neoplastic epithelium in 18 (~30%) of the adenocarcinoma specimens (n=57) with Gleason scores ranging from 5–9, but not in the epithelium of non-neoplastic prostates (n=30) (Figures 1a–c). NFATc1+ cells were also present in the tumor stroma. In addition, we have found NFATc1 expression in the human malignant PC3, LNCaP and DU145 cells, but not in the non-tumorigenic RWPE-1 cells (Figures 1d–g). These results are consistent with previous findings that NFATc1 expression is associated with the initiation, progression, and probably even the metastasis of the various cancers,3 including PCa.7, 20
Inducible NFATc1 activation in prostatic epithelium causes PIN and prostatic adenocarcinoma
To investigate the potential role of NFAT signaling in PCa, we created a mouse model for inducible NFATc1 activation in cells targeted by the PBCre4 (PCre) transgene21 with known expression in the prostatic epithelium. In this system, Cre expression induces the removal of the transcriptional stop cassette in a ROSArtTA (RT) allele22 and the production of rtTA (reverse tetracycline-controlled transactivator). In the presence of doxycycline (Dox), the Dox-rtTA complex binds to the TetO sequence of the TetO-NFATc1Nuc (TN) transgene23 to induce the transcription of NFATc1Nuc (an activated form of NFATc1) (Figure 2a). We refer to mice carrying all three alleles (PCre, RT, TN) as mutants. Their littermates missing any of these alleles cannot have NFATc1 activation, even in the presence of Dox, and thus are regarded as controls. NFATc1Nuc transcripts were detected in Dox-treated mutants, but not in similarly treated controls (Figure 2b).
We treated control-mutant pairs (n=23) with Dox from weaning (P21-postnatal day 21) for variable lengths of time. We found a marked expansion of the prostate lobes in mutants treated for 14 weeks (Figures 2c and d). Although mutants treated for 6 weeks (n=6) did not show marked outward changes, histological analyses showed that they already had PIN recognized by proliferation and stratification of epithelial cells (Figures 2e and f). In total, 96% (22/23) of the mutants with NFATc1 activation for 14 weeks had PCa in the dorsolateral and ventral prostate lobes (Figures 2g and h), whereas all controls had normal epithelium. The majority of human PCa is found in the peripheral zone that is most similar to the mouse dorsolateral lobes. About half of the mutants also developed PCa in the anterior prostate. The remaining half either had PIN or failed to develop any significant neoplastic changes in the anterior prostate. The murine PCa showed an acinar growth pattern, similar to those seen in human prostatic acinar adenocarcinoma.
NFATc1-induced PCa has cellular composition resembling human prostatic adenocarcinoma
Normal prostate gland has a CK5+ (cytokeratin 5) and p63+ basal epithelial cell layer, a CK8+ (cytokeratin 8) luminal epithelial cell layer with AR (androgen receptor) expression, a small number of SYNAP+ (synaptophysin) neuroendocrine cells, and the surrounding myofibroblast cells that are α-smooth muscle actin (SMA)+ (Figures 3a, c, e, g and i). In mutants with NFATc1 activation for 14 weeks starting from P21, tumor expansion primarily came from CK8+/AR+ luminal epithelial cells (Figures 3b, d, f, h and j), a situation similar to the most common type of human PCa. Cytokeratin 5+ basal cells and SYNAP+ neuroendocrine cells were mostly absent from tumor proper and were found in adjacent glands that are either benign or apparently in transition to neoplasia. SMA+ fibromuscular layers surrounding the prostatic glands lost continuity or were absent in many areas in these mutants, likely as the result of invasion of CK8+ cells into the stroma (Figure 3j).
Active proliferation of NFATc1− cells appears to result from a promitogenic microenvironment
In addition to a high percentage of NFATc1+ cells (49.5±4.40%) in the PCa being PCNA+ (proliferating), many neighboring NFATc1− cells (15.8±2.57%%) were also PCNA+ (Figures 4a–c). Interestingly, although 56.6±7.45% NFATc1+ cells were expressing c-MYC, 32.4±4.0% NFATc1− cells also had c-MYC expression in the PCa (Figures 4d–f). Furthermore, 59.1±8.31% NFATc1+ and 16.63±2.11% NFATc1− cells had nuclear phospho-STAT3 (pSTAT3) (Figures 4g–i). STAT3 activation in NFATc1− cells cannot directly result from NFAT transcriptional regulation. Instead, such changes may be due to microenvironmental alterations. These observations are consistent with previous findings that cultured cells expressing an active NFATc1 secrete unidentified and heat labile factors to promote the proliferation of other cells that are NFATc1−.4, 24 Immunoblotting of lysates from normal prostates and NFATc1-induced PCa showed that the level of pSTAT3 was greatly increased in NFATc1-induced PCa, though total STAT3 level was unchanged (Figure 4j).
Increased expression of proinflammatory cytokines and other factors in NFATc1-induced PCa
We selected a number of cytokines and secreted factors for further analyses in the PCa based on our previous findings25 and other studies linking them to NFAT3 and/or PCa.26 By RT–PCR, we showed that NFATc1 activation was accompanied by increased levels of transcripts from Spp1, Saa3, IL6, IL1β, IL1α, Ccl3, Lcn2 and others (Figure 5a), some of which were implicated in promoting PCa progression.27 By immunofluorescent staining, we showed significantly elevated levels of secreted cytokines, such as Spp1 and its receptor CD44, IL6 and IL1β, in the PCa (Figures 5b–i). Spp1 has been reported as an important diagnostic and prognostic biomarker for PCa and some of the NFATc1 oncogenic effects may be channeled through the upregulation of Spp1.27, 28
Tumor progression and survival depend on activation of NFATc1 but not on T-cell functions
To directly test the essential role of NFATc1 activation in tumorigenesis and to examine the involvement of lymphocytes in establishing the tumorigenic microenvironment, we studied the ability of the cells with NFATc1 activation to initiate tumorigenesis in nude mice with absence of T cells. We derived tumor cells from NFATc1-induced murine PCa and showed that ~70% of these cells expressed NFATc1 and the HA (human influenza hemagglutinin) tag fused to the C-terminus of NFATc1 (Figure 6a), but no CD45+ cells were present (data not shown). These cells were injected to the rear flanks of the nude mice. Tumor growth was detected as early as 4 weeks after the injection in the Dox-treated (with NFATc1 activation), but not in the untreated (without NFATc1 activation), recipient mice (Figures 6b–d). To further test the dependency of tumor growth and progression on NFATc1 activation, we stopped Dox treatment in a subgroup of these mice. Existing tumors started to shrink within days after Dox withdrawal (Figure 6d). This trend was reversed when NFATc1 activation was restored with Dox treatment (Figure 6d), indicating a continuous dependency of the PCa on NFATc1 activation, similar to that seen in cases of oncogene addiction.29, 30, 31 Histopathological analyses of tumors revealed that these allografts contained carcinoma with a more solid growth pattern but showed cytological features similar to those seen in original tumors (Figure 6e), including the presence of a large number of NFATc1+/E-Cad+ cells (Figure 6f) and STAT3 activation in both NFATc1+ and NFATc1− cells that intermingled within the tumor proper (Figure 6g). As inflammatory cytokines, such as IL6, are similarly upregulated in the grafts (Figure 6h), the establishment of the inflammatory tumorigenic microenvironment thus appears independent of T cells, but likely dependent on the tumor cells, local tissue resident cells, and other immune cells in this model.
NFAT signaling can overcome androgen deprivation to drive PCa progression
Androgens are critical both for development and function of the prostate gland and for the survival and proliferation of the epithelial cells.32 To determine whether NFATc1-induced PCa would respond to hormone deprivation therapy, we analyzed prostates from 18-week-old mutant mice with NFATc1 activation, as weaning and were either castrated or mock-castrated at 14 weeks of age. PCa samples from castrated and mock-castrated mutants are similar in tumor size and histopathological features (Figures 7a and b). However, the distinct nuclear AR staining seen in the prostates of non-castrated mice was replaced by a more diffused and weaker expression pattern in castrated mice, indicating that castration had effectively reduced AR signaling in prostatic cells (Figures 7c and d). Tumors from both mock-castrated and castrated mice had significant number of PCNA+ proliferating cells, indicating that androgen deprivation had little or no effect on NFATc1-induced PCa and their proliferation (Figures 7e and f).
NFATc1 activation synergizes with the PI3K-AKT pathway to promote PCa progression
Pten is one of the most frequently mutated tumor suppressors in PCa.33, 34 To understand whether and how the NFAT and PI3K-AKT pathways interact in PCa, we generated mice with both Pten deficiency and NFATc1 activation in prostatic epithelia. At 10 weeks of age, most PCre/+;Ptenfl/fl mice with only PTEN deficiency in the prostate epithelium showed enlarged anterior prostates, whereas control and PCre/+;RT/+;TN/+ mice with only NFATc1 activation starting from P21 in prostatic epithelium had no visible tumors. Interestingly, all double mutants (PCre/+;RT/+;TN/+;Ptenfl/fl) with both PTEN deficiency and NFATc1 activation developed significantly larger tumors in all prostate lobes when compared with mice of the same age with either Pten deficiency or NFATc1 activation alone (Figures 8a–d). The average prostate weight in double mutants (6026.24±1946.85 mg) was increased 17.41-fold when compared with the controls (346.85±36.66 mg), 15.45-fold when compared with mice with NFAT activation alone (390.28±73.16 mg), 7.35-fold when compared with Pten null mice (819.14±139.4 mg, Figure 8q). Histopathological analyses revealed that Pten null mice and mice with NFATc1 activation alone had PIN at this time, whereas double mutants already had poorly differentiated prostatic adenocarcinoma (Figures 8e–h). Although levels of pAKT were low in prostates from controls and mice with only NFATc1 activation, increased expression of pAKT was apparent in PCre/+;Ptenfl/fl and PCre/+;RT/+;TN/+;Ptenfl/fl samples, indicating that the PI3K-AKT pathway was activated in prostates with PTEN loss (Figures 8i–l). SMA staining revealed intact myofibroblast layers in the prostates from single mutants but widespread disintegration of the SMA layer in double mutants, consistent with invasion of the epithelial cells into the stroma (Figures 8m–p). These findings reveal that NFATc1 activation synergizes with PTEN-AKT pathway for PCa initiation and progression.
NFATc1 activation overcomes PTEN loss-induced cellular senescence through downregulation of cell cycle inhibitors
It has been shown that senescence has a tumor-suppressive role in PTEN-deficient cells, explaining the long tumor latency in murine models with PTEN-deficient prostate.34 The earlier onset and faster progression of PCa in double mutants suggest that NFATc1 activation may allow the tumor cells to avoid the PTEN loss-induced cellular senescence, resulting in accelerated tumor growth. Therefore, we examined markers of proliferation and senescence in prostates from (PCre/+;Ptenfl/fl), (PCre/+;RT/+;TN/+), and (PCre/+;RT/+;TN/+; Ptenfl/fl) mice. The PCre/+;RT/+;TN/+;Ptenfl/fl tumors had significantly higher levels of proliferation (68.87±18.37%) than the PCre/+;Ptenfl/fl (24.16±6.76%), and PCre/+;RT/+;TN/+ (33.46±3.72%) tumors, as assessed by PCNA staining (Figures 9a–d, m). Furthermore, there was a marked decrease in the expression of the senescence marker p21 in PCre/+;RT/+;TN/+;Ptenfl/fl samples when compared with the PCre/+;Ptenfl/fl mice (Figures 9e–h, arrowhead and inset). p21 staining was predominantly nuclear in PCre/+;Ptenfl/fl prostates (63.6±7.95%). In contrast, nuclear p21 expression was absent in PCre/+;RT/+;TN/+;Ptenfl/fl (4.2±1.30%) prostates, where cytoplasmic p21 was occasionally observed (Figure 9n). Although nuclear p21 is considered as tumor suppressors, cytoplasmic p21 may have antiapoptotic roles and enhance cell survival.35, 36 To further confirm that NFATc1 activation overcomes PTEN loss-induced cellular senescence, we stained for senescence-associated β-galactosidase (SA-β-gal) activity in the prostates. Control and PCre/+;RT/+;TN/+ prostates showed very few senescent cells, 1% and 6.66±0.5%, respectively. In contrast, 65.6±8.7% cells within the PCre/+;Ptenfl/fl prostates were SA-β-gal+. Such SA-β-gal+ cells in the PCre/+;RT/+;TN/+;Ptenfl/fl prostates were markedly reduced to 5.8±1.3% (Figures 9i–l, o), supporting the hypotheses that NFATc1 overcomes Pten-induced cellular senescence by downregulating cell cycle inhibitors.
Discussion
In this study, we have shown higher levels of NFATc1 expression in human PCa specimens and in PCa cell lines when compared with non-neoplastic prostates and non-tumorigenic prostatic cells (Figure 1), consistent with a recent reports of NFATc1 expression in human PCa specimens.7 By using a mouse model for prostate-specific and Dox-inducible NFATc1 activation, we have demonstrated that NFATc1 activation in luminal prostatic epithelial cells causes prostate adenocarcinoma with histopathological features similar to the most common type of human prostate adenocarcinoma (Figures 2 and 3). We further showed that NFATc1 activation promotes PCa by upregulating key oncogenic proteins and by establishing a promitogenic and proinflammatory microenvironment (Figures 4 and 5). We have further demonstrated that NFATc1 activation can overcome PTEN loss-induced cellular senescence, greatly accelerate PCa progression associated with PTEN deficiency (Figures 8 and 9). Our results from the mouse model provide the direct in vivo evidence that NFATc1 can function as a robust oncogene in prostate tumorigenesis.
The upregulation of c-MYC and activation of pSTAT3 in both NFATc1+ and NFATc1− cells cannot be the sole direct results of transcriptional regulation by NFATc1. Instead, in addition to the apparent cell autonomous effects of NFATc1 activation in the regulation of c-MYC, part of the impact from NFATc1 activation appears to go through the production of one or more secreted factors whose existence was first suggested by Neal et al.4 when studying the effects of NFATc1 activation in cultured 3T3-L1 cells. We have demonstrated the increased expression of multiple cytokines (including IL6, IL1, SPP1 and so on, Figure 5) that have the potential to initiate, or at least contribute to, a proinflammatory and promitogenic microenvironment. Multiple recent studies have collectively indicated that SPP1 is one of the four key signatures genes correlated with PCa progression and prognosis.27 Direct transcriptional regulation of SPP1 by NFAT has been reported in arteries,28 consistent with our finding of increased SPP1 levels in mice with NFATc1 activation. Elevated IL6 levels are found in human PCa specimens and even in serum of patients with untreated metastatic or castration-resistant prostate cancer where IL6 levels correlate negatively with tumor survival and response to chemotherapy.37, 38 NFATc1 activation-induced upregulation of IL6 and other proinflammatory cytokines (Figures 4 and 6) can activate the JAK-STAT pathway in both NFATc1+ and NFATc1− cells, as revealed by our data of STAT3 activation in the PCa (Figure 4), leading to promitogenic effects, including the upregulation of c-MYC that is known to be a target of STAT3 regulation.39, 40, 41 Clinical trials with anti-IL6 antibody therapy has not yet demonstrated beneficial effects in advanced PCa.37, 38 Although there may be many explanations, the wide range of cytokine activation in the microenvironment, as seen in the NFATc1-induced PCa, predicts that monotherapy targeting any one of them would not be particularly effective.
As our data support the notion that NFATc1 can be a potent oncogene for PCa through its cell autonomous and non-autonomous effects, we went a step further to ask if and how NFATc1 activation may interact with other commonly occurring PCa mutations. PTEN is an important tumor suppressor and is frequently mutated in PCa.42 By introducing NFATc1 activation to mice deficient for Pten, we have showed synergistic effects of NFATc1 activation and Pten inactivation/Akt activation, as evident by the earlier onset and greater aggressiveness of the PCa in the double mutants. The mechanistic base of such synergism is likely that the introduction of the NFATc1 activation confers the cells the ability to overcome PTEN loss-induced cellular senescence (Figures 8 and 9). Although the detailed mechanism for the anti-senescence effects of NFATc1 activation requires further investigation, it is possible that NFATc1-Interleukins-JAK-STAT3 axis upregulates the expression of SKP2, which in turn, suppresses the expression of p21. This hypothesis is consistent with the observations that SKP2 can be induced by activated STAT3 and that SKP2 negatively regulates p21.43, 44 Regardless of the specific pathways affected, the anti-senescence effects of NFATc1 activation can conceivably enhance the tumorigenic effects of mutations in other genes associated with prostate tumorigenesis.
We have so far not been able to find evidence of metastasis of the NFATc1-induced PCa in mice with NFATc1 activation in the prostate for as long as 5 months. Although it is possible that metastasis may occur if the PCa progresses further, this cannot be determined in the current system we use due to lesions in the ears of the mutant mice that result in the euthanasia of the mice by animal study protocol. The exact cause of these lesions is unknown but appears to be related to ectopic expression of the PCre transgene used. As such, the question of potential metastasis in older mice will be investigated when more specific Cre drivers become available.
Although we have shown that NFATc1 activation drives PCa initiation and progression, we do not expect that genetic mutations of the NFATc1 gene to be a major mechanism for NFATc1 activation. This is because that the ligand-independent activation of NFATc1 requires blocking multiple phosphorylation sites. Random mutations within NFATc1 will most likely inactivate but not activate the protein.3 Instead, the activation of NFATc1 is likely a result of mutations in genes whose protein products act upstream of NFATc1. One of such upstream factors may be TRPV6 that has been linked to human PCa and thought to enhance proliferation and apoptotic resistance through the upregulation of the calcium-calcineurin-NFAT pathway.6 As NFATc1 activation in the prostate has pleiotropic effects on the cells expressing it and on the neighboring cells through alterations in multiple signaling factors in the microenvironment, inhibiting NFATc1 activation could be more effective than targeting one or more of the downstream pathways and factors in treating cancers with NFATc1 activation. The recent findings that calcineurin-NFAT inhibitors suppressed proliferation, migration, and invasion of cultured PCa cells,7 as well as that Silibinin suppressed the PCa cells-induced osteoclastogenesis partially through the inhibition of NFATc110 are encouraging and in broad agreement with data presented in this report. Our in vivo data on oncogene addiction and the ability of NFATc1 activation to continue to drive PCa progression after castration further suggest an oncogenic role of NFAT in castration-resistant prostate cancer and the potential benefits in inhibiting NFAT pathway in fighting PCa.
Materials and methods
Mouse (Mus musculus) strains and Dox treatment
All animal studies were approved by the Washington University Animal Studies Committee and have been conducted according to relevant NIH guidelines. The PBCre4-Cre (PCre/+), ROSArtTA(RT), TetO-NFATc1Nuc (TN) strains and the genotyping methods were described previously.17, 21, 22, 23 For NFATc1Nuc, the substitution of the serines targeted for phosphorylation and dephosphorylation with alanines renders the modified NFATc1 proteins constitutively nuclear and active. For studying interactions between NFAT and PTEN, PCre mice were crossed with Ptenfl/fl mice (The Jackson Laboratory, Bar Harbor, ME, USA) to generate PCre/+;Ptenfl/+ males. These PCre/+;Ptenfl/+ mice were then crossed to RT/RT;TN/+;Ptenfl/fl mice to generate controls and PCre/+;RT/+;TN/+;Ptenfl/fl mutants. Dox was given through drinking water provided ad libitum at 2 mg/ml, starting at P21. Drastic morphological differences makes blinding of specimens from different genotype groups ineffective in this study.
Statistical analyses
We use all available specimens that meet the quality standards. All quantitative data are presented as mean±s.d. Two-tailed t-tests were performed between groups and P<0.05 is considered significant.
Human prostate specimens
Formalin-fixed paraffin-embedded human specimens were obtained from the archives of prostate biopsies in the Department of Pathology and Immunology at Washington University School of Medicine. The human studies protocol was approved by the IRB at Washington University School of Medicine. Tissue Microarrays containing human non-neoplastic prostate specimens and prostatic adenocarcinomas with Gleason scores 5–9 were obtained from Biomax.
Histology, immunostaining and western blotting
Mouse tissues were fixed with 4% paraformaldehyde and embedded in paraffin. Sections of 7 μM were collected and stained with Hematoxylin and Eosin. Immunostaining was performed as described.25 In brief, deparaffinized sections were hydrated and antigen retrieval was done using citrate buffer (10 mM, pH6), using a steamer. These slides were washed with 100 mM glycine for 2 × 10’ and incubated with primary antibodies in humidified chamber overnight at 4 °C. After washing with phosphate-buffered saline with tween 3 × 5’, samples were incubated with secondary antibodies for 1 h at room temperature and washed with phosphate-buffered saline with tween 4 × 5’. After incubation with 4',6-diamidino-2-phenylindole for 5’, the slides were washed again and mounted with Fluoromount G for imaging (Southern Biotech, Birmingham, AL, USA). Primary antibodies used were: from Abcam, Cambridge, UK: rabbit polyclonal Anti-ECadherin (ab53033 1:100), rabbit polyclonal anti-CK5 (ab24647, 1:100), mouse monoclonal anti-p63 (ab53039, 1:100), rabbit polyclonal anti-androgen receptor (ab47570, 1:100), rabbit polyclonal anti-SPP1 (ab8448, 1:100), mouse monoclonal anti-synaptophysin (ab18008, 1:100), rabbit polyclonal anti-IL6 (ab6672, 1:200), rabbit polyclonal anti-c-MYC (ab39688, 1:100); from Life Technologies, Carlsbad, CA, USA: rat monoclonal anti-CD44 (558739,1:100), mouse monoclonal anti-NFATc1 (556602, 1:100), Alexa Fluor 488 and 555-conjugated secondary antibodies (1:1000); from Cell Signaling Technologies, Danvers, MA, USA: rabbit polyclonal anti-Phospho STAT3 (9131, 1:100), rabbit monoclonal anti-STAT3 (4904, 1:50), rabbit monoclonal anti-pAKT (4060 s, 1:100); from Sigma, St Louis, MO, USA: mouse monoclonal anti-αSMA (A2547, 1:100), horseradish peroxidase-conjugated secondary antibodies (1:1000); from Santa Cruz Technologies, Santa Cruz, CA, USA: rabbit polyclonal anti-IL1β (sc7884, 1:100), rabbit polyclonal anti-p21 (sc-471, 1:50); from Developmental Studies Hybridoma Bank, Iowa City, IA, USA: rat monoclonal anti-Cytokeratin 8 (TROMA-1, 1:100); from Bethyl Labs, Montgomery, TX, USA: rabbit monoclonal anti-PCNA (IHC-00012,1:100).
For western blotting, protein extracts were separated by SDS–PAGE, transferred to Immobilon-P membranes (Millipore, Bedford, MA, USA) and probed by appropriate primary antibodies. After incubation with Alexa 680-conjugated goat anti-rabbit IgG (Life Technologies) and IRDye 800CW-conjugated goat anti-mouse IgG (Rockland, Gilbertsville, PA, USA) secondary antibodies, antibody complexes were visualized by an Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA).
RT–PCR
Total RNA was isolated using Trizol reagent (Life Technologies) and purified with an RNeasy Mini Kit (Qiagen, Valencia, CA, USA). cDNA was prepared by using the ThermoScrip RT–PCR System (Life Technologies). PCR conditions were: 95 °C, 4’, 35 × (94 °C 35’’; 55–59 °C, 35’’; 72 °C, 35’’). Primers used for 59 °C annealing were: NFATc1-Fwd AAGAAGATGGTCCTGTCTGG and NFATc1-Rev GTAGTCTGGTACGTCGTAC; IL6-Fwd TGTGCAATGGCAATTCTGAT and IL6-Rev-GGTACTCCAGAAGACCAGAGGA; IL1β-Fwd CTGTGGCAGCTACCTGTGTC and ILβ-R ev TAATGGGAACGTCACACACC. Primers used for 58 °C annealing were: Ccl3-Fwd CTGCCTGCTGCTTCTCCTAC and Ccl3-Rev CCCAGGTCTCTTTGGA-GTCA; IL1α-Fwd CAGTTCTGCCATTGACCATCT and IL1α-Rev CTCCTTGAAGGTGAAGTTGGA; Lcn2-Fwd AAACAGAAGGCAGCTTTACGA and Lcn2-Rev CCTGGAGCTTGGAACAAATG; Gapdh-Fwd CACTCTTCCACCTTCGATG and Gapdh-Rev TGCTGTAGCCGTATTCATTG. Primers used for 56°C annealing were: Saa3-F CCGTGAACTTCTGAACAGCCT and Saa3-R TGCCATCATTCTTTGCATCTTGA; Primers used for 55 °C annealing were: Spp1-Fwd TGGTGCCTGACCCATCTCA and Spp1-Rev GTTTCTTGCTTAAAGTCATCCTTTTCTT.
Cell culture
For primary tumor cell culture, prostates from PCre/+;RT/+;TetO-NFATc1Nucmice, treated for 14 week since P21, were harvested and cut into cubes <1mm3 and cultured in Dulbecco's modified eagle medium-F12 (10% fetal bovine serum, 5% penicillin/streptomycin and 2 μg/ml Dox). Cells grew out of the tumor chunks were fed with fresh media every 2–3 days. A subculture with predominantly epithelial cells was established and expanded for subsequent experiments. RWPE-1, PC3, LNCaP and DU145 cells were originally obtained from the American Tissue Culture Collection (ATCC) and maintained according to ATCC guidelines. PC3, LNCaP and DU145 cells were grown in Rosewell Park Memorial Institute media supplemented with 10% fetal bovine serum. RWPE-1 cells were maintained in keratinocyte-serum-free media supplemented with epidermal growth factor and bovine pituitary extract.
Tumor graft in nude mice
For allografts, 3 × 106 aforementioned cultured tumor cells were injected subcutaneously into lower flanks of 16 male NCr nude mice (6– 8-week-old, Taconic, Hudson, NY, USA). These mice were randomized into four groups. Group 1 was untreated and groups 2–4 were Dox-treated right after tumor cell injection. Group 2 was killed on the 70th day. Dox was stopped for group 3 on the 77th day and the mice were killed on the 84th day as tumors started to recede. Dox was stopped for group 4 on the 84th day and restarted on the 105th day. Group 4 was killed on the 126th day. Tumor volume was determined using a previously described formula.45
Castration
After anesthesia with 80 mg/kg ketamine and 5 mg/kg xylazine, each testis was gently pushed into the scrotum and surgically removed through a 0.5 cm incision. The spermatic cord and vascular plexus were tied with sterile suture. Wound clips were used to close the incision and were removed 1 week after surgery.
Senescence assay
Tissues sections were assessed for senescence by staining for SA-β-gal activity.46 In brief, prostate cryosections were fixed at room temperature for 3 min in 0.2% glutaraldehyde and 2% formaldehyde, rinsed with phosphate-buffered saline and incubated overnight at 37 °C in SA-β-gal staining solution (40 mM citric acid, 40 mM H2NaPO4_2H2O, 5 mM K4Fe(CN)6_3H2O, 5 mM K3Fe(CN)6, 2 mM MgCl2, 150 mM NaCl, pH6.0), containing 1 mg/ml X-gal (US Biochemical, Cleveland, OH, USA).
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Acknowledgements
We thank Drs Gerald Crabtree and Minggui Pan for providing the TetO-NFATc1Nuc mice. FC is supported in part by grants from DoD (PC130118) and NIH (DK087960). ZY is supported in part by NIH R01CA174714. LD is supported in part by grants from NIH (R01CA180006, R01CA178383 and U01HG006517).
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Manda, K., Tripathi, P., Hsi, A. et al. NFATc1 promotes prostate tumorigenesis and overcomes PTEN loss-induced senescence. Oncogene 35, 3282–3292 (2016). https://doi.org/10.1038/onc.2015.389
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DOI: https://doi.org/10.1038/onc.2015.389
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