Abstract
Owing to the absence of the pentose phosphate pathway, the degradation pathway for the ribose moieties of nucleosides is unknown in Archaea. Here, in the archaeon Thermococcus kodakarensis, we identified a metabolic network that links the pentose moieties of nucleosides or nucleotides to central carbon metabolism. The network consists of three nucleoside phosphorylases, an ADP-dependent ribose-1-phosphate kinase and two enzymes of a previously identified NMP degradation pathway, ribose-1,5-bisphosphate isomerase and type III ribulose-1,5-bisphosphate carboxylase/oxygenase. Ribose 1,5-bisphosphate and ribulose 1,5-bisphosphate are intermediates of this pathway, which is thus designated the pentose bisphosphate pathway.
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Acknowledgements
This study was funded by the Core Research for Evolutional Science and Technology program of the Japan Science and Technology Agency to H.A. within the research area 'Creation of Basic Technology for Improved Bioenergy Production through Functional Analysis and Regulation of Algae and Other Aquatic Microorganisms'. R.A. is a Research Fellow of the Japan Society for the Promotion of Science.
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H.A., T.I., T.S. and R.A. designed the work; R.A. carried out the experiments; R.A., T.S. and H.A. wrote the manuscript.
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Aono, R., Sato, T., Imanaka, T. et al. A pentose bisphosphate pathway for nucleoside degradation in Archaea. Nat Chem Biol 11, 355–360 (2015). https://doi.org/10.1038/nchembio.1786
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DOI: https://doi.org/10.1038/nchembio.1786
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