Abstract
A conserved region of a polygalacturonase (PG) gene from Penicillium griseoroseum was PCR amplified and used to screen a genomic library from this fungus. The nucleotide sequence of the isolated clone (pggI) consisted of 1497 bp, including a coding region of 1251 bp. This region potentially encodes a protein of 376 amino acids, and is interrupted by two introns. Extensive homology was observed between this protein and several fungal endopolygalacturonases. DNA hybridization analyses revealed that there is a low copy number of pggI in the P. griseoroseum genome, probably one or two copies.
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Ribon, A., Coelho, J., de Barros, E. et al. Cloning and characterization of a gene encoding the endopolygalacturonase of Penicillium griseoroseum . Biotechnology Letters 21, 395–399 (1999). https://doi.org/10.1023/A:1005419225412
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DOI: https://doi.org/10.1023/A:1005419225412