Abstract
Mitochondria are complex organelles that undergo constant fusion and fission in order to adapt to the ever-changing cellular environment. The fusion/fission proteins, localized in the inner and outer mitochondrial membrane, play critical roles under pathological conditions such as acute brain injury and neurodegenerative diseases. Post-translational modifications of these proteins tightly regulate their function and activity, ultimately impacting mitochondrial dynamics and their efficiency to generate ATP. The individual post-translational modifications that are known to affect mitochondrial dynamics include SUMOylation, ubiquitination, phosphorylation, S-nitrosylation, acetylation, O-linked N-acetyl-glucosamine glycosylation, ADP-ribosylation, and proteolytic cleavage. Under stress or pathologic conditions, several of these modifications are activated leading to a complex regulatory mechanism that shifts the state of the mitochondrial network. The main goal is to accommodate and adapt the cellular bioenergetics metabolism to the energetic demand of the new extra- and/or intracellular environment. Understanding the complex relationship between these modifications on fusion and fission proteins in particular pathologic stress or diseases can provide new promising therapeutic targets and treatment approaches. Here, we discuss the specific post-translational modifications of mitochondrial fusion/fission proteins under pathologic conditions and their impact on mitochondrial dynamics.
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Introduction
Mitochondria are subcellular organelles that are involved in a variety of essential cellular functions, the most prominent role being to supply the cell with metabolic energy in the form of ATP [1]. The type of function mitochondria performs is reflected in its structure, more specifically its unique ability to undergo fusion and fission. This dynamic behavior allows mitochondria to respond to physiological and pathological stimuli, resulting in their fragmentation into smaller organelles or forming elongated, interconnected forms [2]. Fusion mixes solutes, metabolites, and proteins in newly formed mitochondria and helps to promote a healthy environment and counter post-stressful conditions. Fission functions to create new smaller mitochondria when needs arise but also performs damage control by allowing removal of dysfunctional mitochondrial segments [3].
A group of guanosine triphosphatases (GTPases)-dependent proteins mediate fusion and fission. These proteins work in unison to quickly divide and fuse the double lipid bilayers of the mitochondrial membranes. Fusion of the outer membrane is mediated by two core proteins, mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2), while inner membrane fusion is mediated by a singular protein, mitochondrial dynamin-like GTPase encoded by optic atrophy 1 gene (Opa1). Fission, on the other hand, is mediated by dynamin-related protein 1 (Drp1) and Drp1 regulating proteins mitochondrial fission 1 (Fis1), mitochondrial fission factor (Mff), and mitochondrial dynamics protein 49/51 (MiD49/51) [4]. These regulatory proteins are required for Drp1 recruitment to the mitochondria where it is then responsible for outer membrane division [5]. Due to the mitochondrial highly dynamic environment, the activity of these proteins is tightly regulated by proteolysis and post-translational modifications that include SUMOylation, ubiquitination, phosphorylation, S-nitrosylation, acetylation, and O-linked-N-acetyl-glucosamine glycosylation [6,7,8]. Fine tuning of the mitochondrial fusion/fission process by post-translational modifications plays a central role not only in facilitating normal mitochondrial function but also is crucial in cellular bioenergetic stress responses to various pathologic conditions, such as neurodegenerative diseases, ischemia, and traumatic brain injury (TBI). Here, we discuss post-translational modifications of fission and fusion proteins and how they alter mitochondrial dynamics and functions in response to pathologic stress in the brain.
SUMOylation and Regulation of Mitochondrial Dynamics
The post-translational modification, SUMOylation, involves covalent attachment of small ubiquitin-like modifiers (SUMOs) to lysine residues of substrate proteins via an enzyme cascade similar to that of the ubiquitination pathway. Unlike polyubiquitination at the lysine 48 residue that targets proteins for degradation, SUMOylation can alter protein function, activity, stability, and subcellular localization offering an effective way to rapidly adapt to the stress-induced changing environment. Mammals have four SUMO paralogs (SUMO1–4) that are conjugated to target proteins by a process that is controlled by a heterodimer of SUMO-activating enzyme subunits 1 and 2 (SAE1/2), a single SUMO-specific conjugating enzyme Ubc9 (E2), and several E3 ligases [9, 10]. SUMO-1 was identified as a covalent protein modifier of the nuclear pore protein RanGAP1 (Ran-GTP-ase-activated protein 1) [11]. SUMO-2 and SUMO-3 differ from each other by only three N-terminal residues and are referred to as SUMO-2/3. SUMO-4 isoform has been proposed from DNA sequence analysis, and the conjugation of a mature form of SUMO-4 has been reported in conditions of extreme cellular stress [12].
Recently, a new mitochondrial SUMO-3 ligase was identified [13]. The mitochondrial-anchored protein ligase (MAPL) functionally stabilizes ER/mitochondrial contact sites that act as hotspots for mitochondrial constriction, calcium flux, cristae remodeling, and cytochrome c release. MAPL can be a crucial component in regulation of mitochondrial functions and events related to mitochondrial interaction with other organelles since a large number of unidentified mitochondrial SUMO targets were detected [13].
SUMOs are de-conjugated from proteins by sentrin-specific proteases (SENPs) (for review see [14, 15]). In mammals, there are six SENPs. SENP1 and SENP2 remove SUMO1 and SUMO-2/3 while SENP3 and SENP5 are more specific for the removal of SUMO-2/3 [16]. Thus, the level of SUMOylated proteins in cells is determined by both, the activity of enzymes that perform SUMO modifications and the activity of de-SUMOylating SENPs isopeptidases.
Under stress, the SUMOylation of proteins increases. This results in the adjustment of cells to changes in environmental conditions yielding a protective effect. For example, during hibernation that convenes a robust resistance to severe reduction of blood flow (state resembling ischemic conditions), there is a marked increase in protein SUMOylation in the brain with concomitant loss of free SUMO [17].
A number of studies have reported that in experimental models of ischemia, there is a global increase in SUMOylation, especially in the brain, and that it is associated with a protective cellular response [18,19,20,21]. Although it was reported that several mitochondrial proteins show SUMOylation as a post-translational modification [22,23,24], Drp1 has been reported as the only mitochondrial fission protein that is regulated by SUMOylation. Compared to other mitochondrial proteins that regulate fusion and fission, the majority of Drp1 is cytoplasmic. Therefore, the translocation to the mitochondria is crucial for the mitochondrial fragmentation process.
As a physiological response, mitochondrial fission is triggered during the cell cycle in the G2/M phase [25]. The SUMO protease SENP5 translocates from the nucleoli to the mitochondrial membrane and deSUMOylates Drp1. This is followed by mitochondrial fragmentation during mitosis [25]. Interestingly, the same laboratory reported that overexpression of SENP5 catalyzes the cleavage of SUMO1 from a number of mitochondrial substrates, including Drp1, leading to the reduction of mitochondrial fragmentation. Silencing of SENP5 resulted in fragmented mitochondria, promoting fission [23]. These seemingly opposing results might reflect a differential effect of Drp1 SUMOylation depending on the particular changes in intracellular environment, and possible synergistic effect of pathways that lead to differential post-translational modifications of Drp1 (Fig. 1).
Similarly, sustained, low activity of SENP2 triggers pathologic changes in the brain [26]. The knockout of SENP2 increased levels of SUMO1 association with Drp1 causing mitochondrial fragmentation and ultimately severe developmental abnormalities [26]. Overexpression of SENP2 prevented the SUMO1-induced accumulation of Drp1 to the mitochondria and also decreased the SUMO1-induced accumulation of Drp1 in the cytoplasm. Thus, suggesting that Drp1 reduction mediated by SENP2 is caused by protein degradation rather than decreased targeting to mitochondria [26].
Interestingly Drp1 is also modified by SUMO2/3. SUMO2/3-ylation acts to separate Drp1 from the mitochondria, ultimately promoting fusion or a less fragmented population of these organelles. DeSUMOylation of SUMO2/3 by SENP3 enhances Drp1 localization with mitochondria promoting apoptosis and fission. Oxygen glucose deprivation (OGD), an in vitro model of ischemia, downregulates expression of SENP3 during the OGD insult, which results in a shift toward SUMOylated Drp1 and dissociation from the mitochondria. However, following re-oxygenation, the SENP3 levels recover allowing deSUMOylation of Drp1, which leads to Drp1 localization to mitochondria and promotes fragmentation and cytochrome c release [27].
The balance and regulation between conjugations of SUMO1 versus SUMO2/3 to Drp1 still remains unclear. However, SUMO1 generally does not exhibit increase in acute stress conditions, which might suggest that SUMO2/3 conjugation to Drp1 has precedence in ischemia and brain injury in the attempt to ensure cell survival [23].
Thus, there is also a seemingly opposite effect of Drp1 SUMOylation on mitochondrial fission between SUMO1 and SUMO2/3 modifications. The final effect of Drp1 modifications on its functional outcome can depend on the combinations of several post-translational modifications, depending on the environmental changes that can trigger different signaling pathways. Therefore, when assessing the possible functional outcome of post-translational modifications in studied pathologic stress, one needs to screen for possible combinations of different modifications rather than determine only one particular change in the modification of the protein of interest.
SUMOylation is also involved in the regulation of Parkin, a cytosolic E3 ligase that carries out the attachment of ubiquitin monomers to proteins located at the outer mitochondrial membrane and facilitates mitochondria specific autophagy (mitophagy). Parkin is SUMO1-ylated, which appears to enhance its ubiquitination activity and ultimately mitophagy [28]. This increased activity affects downstream targets of Parkin, such as the mitochondrial fusion proteins Mfn1/2 [29]. Increased ubiquitination of Mfn1/2 targets them for degradation and ultimately a reduction in fusion activity. It has yet to be determined whether in brain ischemic conditions there is a greater amount of SUMO1-Parkin, which could lead to degradation of Mfn1/2 and subsequent decreased fusion.
Data from studies examining changes in mitochondrial proteins suggest that levels of SUMOylation are primarily regulated not by the activity of the SUMOylation process (E1, E2, E3 enzymes) but rather by controlling the deSUMOyation mechanisms via altering specific SENPs activities. Thus, the targeted alterations of distinct SENP levels provide an efficient mechanism for the changing of SUMOs metabolism and could offer a promising therapeutic approach. However, generally, the SUMOylation of cellular proteins is regulated through a complex interplay between SUMOylation and other post-translational modifications through modification of the proteins involved in their enzymatic pathways [28, 30].
Of note, increased activity of the SUMOylation/deSUMOylation cycle can also have potentially adverse effects on mitochondrial functions, particularly under bioenergetic stress. This is because the activity of the E1 enzyme is an ATP-dependent process that leads to consumption of ATP and generation of AMP and pyrophosphate (PPi) [28]. Prolonged, increased levels of intracellular AMP and PPi can inhibit mitochondrial oxidative phosphorylation by interfering with mitochondrial adenine nucleotide transporter, thus compromising mitochondrial ATP production [31]. Furthermore, depleting mitochondria of adenine nucleotide leads to increased sensitivity to calcium-induced mitochondrial permeability transition pore opening [32].
In summary, SUMOylation regulates mitochondrial fusion/fission via several pathways. Drp1 SUMOylation/deSUMOylation directly alters availability of Drp1 at the mitochondrial membrane while Parkin SUMOylation indirectly increases Mfn1/2 degradation resulting in decreased mitochondrial fusion. Furthermore, protein levels of SENPs and availability of ATP can alter the activity of the SUMOylation/deSUMOylation cycle. During stress conditions, it is unclear how these various pathways are altered; however, future studies in this field would gain a possible therapeutic advantage.
Ubiquitination
Ubiquitin is a small regulatory protein that can be reversibly added to lysine residues of proteins by ubiquitin ligases. The addition of this modification has broad effects on protein function and stability depending on whether a monomer or polymer of ubiquitin is attached. The addition of a monomer regulates protein activity while the formation of an ubiquitin chain to a single lysine targets the protein to the proteasome for degradation [33]. It is reported that the core proteins involved in mitochondrial dynamics, Drp1, Mfn1/2, and Fis1, can be regulated by ubiquitination. Drp1 is ubiquitinated by either MARCH5 or Parkin, both of which are E3 ubiquitin ligases [34,35,36]. Fis1 is ubiquitinated by MARCH5 while Mfn1/2 by Parkin and MARCH5 [29, 37].
The mitochondrial E3 ubiquitin ligase MARCH5 was identified as a protein of the mitochondrial outer membrane that interacts with Mfn2 and ubiquitinated forms of Drp1 [34]. Originally, it was proposed that ubiquitination by MARCH5 promotes the formation of long tubular mitochondria as a result of Mfn2-dependent fusion [34, 38]. However, later, an opposite functional outcome of MARCH5-induced Drp1 ubiquitination was demonstrated, suggesting that MARCH5 may support fission by facilitating the subcellular trafficking and recruitment of Drp1 to actual sites of mitochondrial division [35]. MARCH5-dependent ubiquitination of Mfn1 was significantly elevated under mitochondrial stress conditions and with an increase in acetylated Mfn1. Thus, it may reflect mitochondrial adaptation to stress and mitochondrial quality control [39].
Parkin functions as an E3 ubiquitin ligase and it translocates from the cytosol to damaged or dysfunctional mitochondria to promote their degradation by mitophagy [29, 40]. However, Parkin can also affect mitochondrial fusion/fission dynamics. In primary mouse neurons, downregulation of Parkin lead to an increase in mitochondrial fragmentation and a decrease in ATP production [41]. Normally, Parkin interacts with Drp1 leading to its ubiquitination and promoting its proteasome-dependent degradation [42]. This suggests downregulation of Parkin would decrease the proteasome-dependent degradation of Drp1, leading to an increase in mitochondrial fission. In contrary, another report showed that Parkin does not interact with Drp1 but promotes ubiquitination and consequent degradation of Mfn1 [29]. Both studies used genetic manipulation to alter the expression levels of Parkin and immunoprecipitation technique to show Drp1 interaction with Parkin. Wang et al. [42] presented positive Drp1 and Parkin interaction using 293 T cells, whereas a lack of Drp1 interaction with Parkin was shown in SH-SY5Y cells [29]. Thus, it seems there are other unknown factors that can affect the Parkin-dependent Drp1 ubiquitination and consequently the functional effect on mitochondrial dynamics. Further experiments are needed, preferentially carried out with primary neuronal cells, to shed more light on the effect of Parkin and Drp1 interaction and its impact on the mitochondrial fusion/fission equilibrium in brain cells.
There is also a complex interplay relationship between ubiquitination and phosphorylation. It was noted that Mfn2 can be phosphorylated by both PTEN-induced putative kinase 1 (PINK1) and c-Jun N-terminal kinase (JNK) under different stress conditions [43]. The phosphorylation on Mfn2 by JNK recruits ubiquitin ligase, Huwe1, triggering Mfn2 degradation and mitochondrial fragmentation, whereas phosphorylation of Mfn2 on the PINK1 site contributes to recruitment of Parkin to the mitochondria [44].
In cerebral ischemic insult models, it remains unclear how ubiquitination of Drp1, Fis1, and Mfn1/2 are altered. However, it is understood that in global cerebral ischemia, there is a general increase in ubiquitinated proteins in the brain [45, 46]. Under these conditions, ubiquitination of mitochondrial proteins are most likely affected, probing future studies in this area.
Drp1 Phosphorylation
Phosphorylation is a well-studied and characterized post-translational modification found typically on serine, threonine, or tyrosine residues. So far Drp1 is the only protein among fusion and fission proteins that was found to be regulated by phosphorylation. Two sites are phosphorylated on Drp1 (P-Drp1), Ser616 of human Drp1 isoform 1 (equivalent to Ser585 in rat Drp1 or ser579 in human Drp1 isoform 3), and Ser637 human Drp1 (equivalent to Ser600, 617, and 656, depending on species and splice variants), which upon phosphorylation usually exhibit opposing effects on Drp1 associated mitochondrial fragmentation. Protein kinase A (PKA) phosphorylates at Ser637 under conditions of nutrient starvation and leads to inhibition of fission [47]. In contrast, phosphorylation by Ca2+/camodulin-dependent protein kinase-1α (CaMK1α) or Rho-associated coiled-coil-containing protein kinase 1 (ROCK1) at the same serine residue increases Drp1 translocation to the mitochondria and enhances fission [48, 49] (Fig. 2). The calcium-dependent protein phosphatase, calcineurin (CaN), removes phosphate groups at the Ser637 site and promotes fission in the model of Huntington’s disease (HD) [50]. The specific pathological conditions and mechanisms leading to opposing functional outcome of phosphorylation at the same serine residue of the Drp1 remain so far elusive and need further investigation.
The cyclin-dependent kinase 1 (CDK1)-cyclin B complex phosphorylates Drp1 at Ser616. During mitosis, P-Drp1 (Ser616) enhances mitochondrial fragmentation and facilitates the proper distribution of mitochondrial mass into daughter cells [51]. Under oxidative stress conditions, protein kinase C δ (PKCδ), phosphorylates the same serine, resulting in increased mitochondrial fragmentation (Fig. 2) [52].
Ischemia and traumatic brain injury usually leads to an enhanced fragmented state of neuronal and glial mitochondria [53,54,55,56]. The model of focal cerebral ischemia induced by middle cerebral artery occlusion (MCAO) resulted in a progressive increase in P-Drp1 at site Ser637 reaching a peak at 2 days of recovery. At this recovery time point, there was increased co-localization of Drp1 and mitochondrial cytochrome oxidase suggesting an increase in mitochondrial fission mediated by CaMK1α [49]. However, mitochondrial fission was not quantified nor the phosphorylation of Ser616, which also regulates fission dynamics. Additionally, the global cerebral ischemic model exhibited enhanced mitochondrial fission but was mediated through the phosphorylation of Ser616 rather than Ser637 [53, 57].
Recently, it was suggested that the PTEN-induced putative kinase 1 (PINK1) could also affect the fusion/fission dynamics following ischemic insult [57]. There was an accumulation of PINK1 in the vulnerable CA1 sub-region of the hippocampus as well as an increase in P-Drp1 levels (Ser616, but not Ser637) following cerebral ischemic insult. Administration of PINK1-siRNA caused a further increase in P-Drp Ser616 and oxidative DNA damage [57]. The authors suggested that PINK1 could affect the activity of CDK1/cyclin B kinase and inhibit the fission process. However, PINK1 accumulation in post-cerebral ischemic tissue is probably due to excessive fission that leads to large number of small non-functional mitochondria [58] which triggers the mitophagy process. PINK1 recruits Parkin, an E3 ubiquitin ligase, whose activity leads to the ubiquitination of mitochondrial outer membrane proteins and triggers the removal of damaged mitochondria [35, 58, 59]. This process leads to decreased reactive oxygen species (ROS) production and reduction of oxidative damage to DNA. Thus, knocking down PINK1 can inhibit mitophagy and ultimately increase ROS production and P-Drp1 levels leading to more extensive post-ischemic fission. Thus, the increase in PINK1 levels is probably the result and not the cause of increased mitochondrial fission.
Nevertheless, it still remains unclear how under particular pathologic conditions mitochondria regulate which Drp1 serine residue (Ser616 or Ser637) is phosphorylated and for what duration of time. It is possible that the functional outcome of a particular Drp1 serine phosphorylation depends on other concomitant post-translational modification as SUMOylation or S-nitrosylation.
S-Nitrosylation
Nitric oxide (NO) is an important second messenger that is generated through the oxidation of l-arginine by nitric oxide synthases (NOS) in the presence of NADPH and oxygen, and is implicated in mechanisms of neuronal cell survival and death [55, 60]. NO represents a reactive nitrogen species (RNS) and signals primarily through formation of S-nitrosothiols (SNOs), representing S-nitrosylation [61]. Furthermore, NO reacts readily with superoxide generating highly reactive peroxynitrate, which can nitrate tyrosine residues to form nitrotyrosine adducts [61]. These modifications can alter protein structure and function, as well as affect protein aggregation. The cysteine residue (Cys644) of Drp1 can be S-nitrosylated resulting in increased Drp1 activity by its dimerization [5]. The ultimate outcome is extensive mitochondrial fission leading to neuronal damage. A mutation of Cys644 has been shown to prevent mitochondrial fragmentation and inhibit the neurotoxicity of NO [62]. However, these findings were challenged by another study showing S-nitrosylation of Drp1 had no impact on Drp1 oligomerization and did not affect its enzymatic activity [63]. Furthermore, it was postulated that there is interplay between S-nitrosylation and phosphorylation of Drp1 [63]. It was reported that Drp1 S-nitrosylation is a modification that is required for phosphorylation of Drp1 at Ser616 by PKCδ or CDK1 (Fig. 3). Thus, treatment of cells with an NO donor enhanced Drp1 phosphorylation at Ser616 resulting in increased translocation to the mitochondria and ultimately fragmentation [63]. Ischemic stroke and neurodegenerative diseases, such as Alzheimer’s, generate NO in excess, which results in increased RNS and S-nitrosylation modifications [64, 65].
S-nitrosylation also regulates the protein activity of Parkin, an E3 ubiquitin ligase. This modification on Parkin abolishes its ubiquitin ligase activity and as a result has negative effects on the process of mitophagy. In addition, S-nitrosylation of Parkin can cause p53-mediated apoptosis in neuronal cells and may contribute to some idiopathic forms of Parkinson’s disease [66]. Consequently, under pathologic stress conditions, extensively fragmented mitochondria may have decreased quality control and degradation leading to an increase in free radical production and cellular damage.
Since NO-induced S-nitrosylation is considered a mechanism that regulates enzymatic activity, the removal of NO groups from cystein thiol (de-nitrosylation) acts as a control to this modification. This is an important but less studied process of NO-induced post-translational modifications. De-nitrosylation has been shown to be catalyzed by two enzymes in vivo (for review see [67, 68] ). These cellular systems were identified as physiological de-nitrosylases: the S-nitroglutathione reductase (GSNOR) system and the thioredoxin (Trx) system [67]. GSNOR is comprised from glutathione (GSH) and GSH-dependent formaldehyde dehydrogenase and class III alcohol dehydrogenase (ADH5). The Trx system is comprised of Trx and Trx reductase (TrxR) [68].
As mentioned above, excess production of NO can generate two different protein modifications: S-nitrosylation due to a reaction of NO with cysteine thiol groups and formation of 3-nitrotyrosine that is generated by the reaction of peroxynitrate with tyrosine residues [69]. Peroxynitrate is a product of NO and superoxide. Thus, the levels of NO depend not only on the activity of nitric oxide synthase (NOS) but also on the rate of superoxide generation that will “scavenge” NO and produce peroxynitrate. Interestingly, we found that following global cerebral ischemia, the 3-nitrotyrosine immunoreactivity was significantly higher in ischemia-resistant hippocampal sub-regions (CA3 and DG) where the mitochondria in neurons were able to refuse. In contrary, within the ischemia vulnerable CA1 neurons, with excessively fragmented mitochondria, the 3-nitrotyrosine immunoreactivity was significantly lower; suggesting higher levels of Drp1 S-nitrosylation, probably leading to permanently fragmented mitochondria [53]. It is still unclear whether 3-nitrotyrosine modifications play significant role in regulation of mitochondrial dynamics.
Acetylation
Acetylation of proteins is a common post-translational modification that has considerable impact on their activity and expression. Enzymatic acetylation of histones and non-histone proteins is dependent on the availability of acetyl CoA [70,71,72,73,74]. This modification is tightly regulated by two opposing families of enzymes, histone acetyltransferases (HATs) and histone deacetylases (HDACs).
Post-translational lysine acetylation of proteins is highly reversible. In humans, there are 18 potential enzymes that remove the acetyl group from lysine and are characterized into four classes: class I HDACs (HDACs 1,2,3 and 8), class II HDACs (HDACs 4,5,6,7,9 and 10), class III HDACs (sirtuin family: SIRT1-SIRT7), and class IV HDACs (HDACs 11) [75]. HDACs also possess substrate specificity and can deacetylate non-histone proteins [76, 77]. Sirtuins (SIRT1–7), the class III HDACs, require NAD+ for their catalytic activity and are known to deacetylate non-histone proteins [75, 78]. The cellular compartmentalization of SIRTs is diverse. SIRT1, SIRT6, and SIRT7 are predominantly nuclear proteins [79], SIRT2 is considered a cytosolic enzyme [80], and SIRT3–5 are localized to the mitochondria [81]. However, SIRT4 acts rather as an ADP-ribosyl transferase and not as a deacetylase [81]. Furthermore, it was shown that SIRT5 can act as a desuccinylase [82, 83] and also as a lysine deglutarylase [84]. However, so far these types of lysine residue modifications were not identified in proteins regulating mitochondrial dynamics.
HATs are broadly categorized into type A, nucleic HATs, or type B, cytoplasmic HATs [70, 71]. Type A HATs are transcription related and sub-classified into five families [77]. The cytoplasmic type B HATs are responsible for deposition-related acetylation of free histone substrates in the cytoplasm during the process of chromatin assembly [85].
Recently, it was reported that there is a mitochondria specific acetyltransferase, general control of amino acid synthesis 5-like 1 (GCN5L1), that counters the activity of mitochondrial deacetylase SIRT3 [86]. GCN5L1 promotes acetylation of SIRT3 respiratory chain targets and reverses global SIRT3 effects on mitochondrial protein acetylation [86]. GCN5L1 is a non-transmembrane globular protein located within mitochondria in either the intermembrane space or matrix-soluble fractions [86].
Interestingly, the level of mitochondrial protein acetylation, controlled by GCN5L1 and SIRT3, also affects the mitophagy process [87]. This mechanism is independent of the E3-ligase Parkin-mediated mitophagy. However, the mitochondrial targets that trigger this program were not identified. Since GCN5L1 is classified as an intra-mitochondrial protein, it is difficult to envision how the acetylation of an intra-mitochondrial protein is affecting the interaction between cytosolic mitophagy components and the outer mitochondrial membrane proteins. However, although not functionally characterized in autophagy, a cytosolic fraction of GCN5L1 (also named BLOC1 component BLOS1) has been shown to be part of a protein complex involved in protein transport to lysosomes [88]. These finding also support the hypothesis that mitochondrial protein lysine deacetylation enables lysine ubiquitination. Thus, acetyl transferases and ubiquitin ligases could compete for certain lysine residues of the target protein.
Under pathological stress, Opa1 is hyperacetylated and this post-translational modification reduces its GTPase activity. SIRT3 deacetylates Opa1 and re-activates this enzyme leading to increased mitochondrial fusion [8]. The acetylation status of Mfn1 is also crucial for mitochondria under stress conditions. Under metabolic stress induced by fasting (glucose removal), Mfn1 is deacetylated by HDAC6, which induces mitochondrial hyperfusion, an attempt to preserve structure [89]. HDAC6 deacetylates Mfn1 at lysine residue (K222), leading to increased Mfn1 activity toward fusion. Surprisingly, it was also shown that under mitochondrial stress conditions induced by exposing cells to antimycin A, a respiratory Complex III inhibitor, acetylated Mfn1 levels increase [39].
Interestingly, the acetylation at lysine residue K491 promoted the interaction of MARCH5 with Mfn1. As a result, MARCH5 binds to Mfn1 leading to its subsequent ubiquitination. Furthermore, it was reported that increased ubiquitination was dependent on increased acetylation of Mfn1 as well [39]. Ultimately, this resulted in Mfn1 degradation by the ubiquitin-proteasome mechanism. This system is an important quality control check for mitochondria under stress conditions. Thus, there are acetylation modifications of Mfn1 induced by mitochondrial stress that have opposing functional outcomes on the mitochondrial dynamics. Exactly how these two regulation states interact with one another under stress remains unclear and needs to be further examined.
Another example of the complex interplay between different post-translational protein modifications was reported by Verdone et al. [90]. A decrease in poly-ADP-ribosylation due to pharmacological inhibition of poly (ADP-ribose) polymerase (PARP) activity or by overexpressing poly-(ADP-ribose) glycohydrolase (PARG) leads to decrease of global histone H3 and H4 acetylation [90]. Since the global acetyltransferase activity did not changed, this suggests that poly-ADP-ribosylation has a role in the regulation of histone deacetylase activity [90].
HDAC inhibitors have neuroprotective, neurotrophic, and anti-inflammatory properties [91]. Following ischemic brain injury, reduced histone acetylation was found at lysine residues, which were restored with HDAC inhibitors resulting in neuroprotection [92,93,94,95,96]. Similarly, nicotinamide (Nam) that inhibits the NAD+-dependent deacetylases, sirtuins, [91], and PARP1 activity, also showed significant protection against acute brain damage (for review [78]).
Together, this data suggests that targeting acetylation statuses of mitochondrial fusion proteins and histones has the ability to alter mitochondrial dynamics and overall function. Therapeutically, this would represent an efficient approach for neuroprotection.
O-GlcNAcylation
O-linked N-acetyl-glucosamine glycosylation (O-GlcNAcylation) represents a dynamic modification of serine and threonine hydroxyl moieties on nuclear and cytoplasmic proteins by O-linked N-acetylglucosamine, termed O-β-GlcNAc or simply O-GlcNAc. Two enzymes regulate protein O-GlcNAcylation, O-GlcNAc-transferase catalyzes the addition of O-GlcNAc while N-acetyl-glucosaminidase (NAG) remove O-GlcNAc residues [97].
O-GlcNAc is particularly relevant to chronic diseases including diabetes, cardiovascular disease, neurodegenerative diseases, and cancer. O-GlcNAc cycles rapidly and the cycling rates are similar to those seen for protein phosphorylation. Interestingly, O-GlcNAcylation and phosphorylation sites overlap which results in opposing functions. O-GlcNAc and O-phosphate exhibit a complex interplay on signaling, transcriptional, and cytoskeletal regulatory proteins within the cell [97]. A shorter splice variant of O-GlcNAc-transferase has been identified that exhibits preferred mitochondrial localization [98, 99]. Increased O-GlcNAcylation of mitochondrial fusion protein Opa1 was observed in cells exposed to high glucose causing mitochondrial fragmentation and inhibition of Complex IV activity [100]. Furthermore, hyperglycemic conditions also increased O-GlcNAcylation of Drp1 protein [7]. An overall increase in O-GlcNAcylation actually decreased phosphorylation of Drp1 at Ser637 leading to increased GTP binding that allows for the translocation of Drp1 to the mitochondria [7]. It has been shown that various stress stimuli elevate global levels of O-GlcNAcylation, which can account for the increased mitochondrial fragmentation observed under these conditions [101]. O-GlcNAcylation thus represents another post-translational modification that contributes to regulation of mitochondrial dynamics. However, further research is needed to understand how this modification specifically plays a role in acute and neurodegenerative brain injury.
ADP-Ribosylation
ADP-ribosylation refers to the transfer of one or more ADP-ribose units from NAD+ onto target proteins as a post-translational modification. ADP-ribose groups can be attached either singularly (mono-ADP-ribose) or as multiples (poly-ADP-ribose chain) catalyzed by the poly-ADP-ribose polymerases (PARP) family [102, 103]. Several amino acids can be ribosylated such as glutamate, aspartate, lysine, arginine, and cysteine [104]. Several enzymes, depending on whether they cleave mono- or poly-ADP-ribose, can mediate cleavage of ADP-ribose. ADP-ribosylhydrolase 1 (ARH1) removes single ADP-ribose groups from conjugated amino acids while ARH3 cleaves the ribose-ribose bond between ADP-ribose subunits in poly-ADP-ribose [105]. Poly-ADP-ribose glycohydrolase (PARG) is also responsible for cleaving ribose-ribose bonds between subunits. Another set of ADP-ribosylhydrolases, MacroD1, MacroD2, and TARG1 are responsible for removing remaining ADP-ribose units from polymer chains as well as single ADP-ribose groups that are conjugated to acidic amino acid residues [105]. As a result, this modification has diverse effects on protein stability/activity.
Immunoprecipitation and mass spectrometry identified mitochondrial fusion proteins Opa1 and Mfn1/2 to be poly-ADP-ribosylated [106, 107]. Mass spectrometry also recognized mitochondrial fission protein Drp1 to be ADP-ribosylated [108]. It remains unclear which specific PARP ADP-ribosylates these proteins; however, PARP1 has been suggested to be localized to the mitochondria, which could be responsible for ribosylation of these proteins [109, 110]. Removal of these ADP-ribose units is possibly mediated by mitochondrial localized ARH3 [111]. Although these proteins have been identified as ADP-ribosylated, it remains unclear how this alters functionality or activity. Post-ischemic injury shows an increase in poly-ADP-ribose levels in the hippocampus, which could drive the increase of this modification on these proteins [112]. However, further research is needed to understand how this modification specifically plays a role in acute and neurodegenerative brain injury.
Proteolytic Cleavage
Proteolysis is a modification involving the breakdown of proteins into smaller polypeptides or amino acids. This modification varies from the modifications mentioned above mainly due to its irreversibility. Cleavage of proteins can result in inactivation, activation, or even altered protein function [113]. Among the proteins regulating mitochondrial fusion, Opa1 activity is directly regulated by proteolytic cleavage. Thus, Opa1 fusogenic ability is modulated by proteolytic processing and depends on the interaction of both the long (non-cleaved) and short, cleaved forms [114,115,116].
Opa1 was demonstrated to control both mitochondrial fusion and cristae morphology [114, 117,118,119,120,121,122]. This protein is localized in the mitochondrial intermembrane space in soluble forms or is attached to the inner mitochondrial membrane [114, 123]. After being imported into the mitochondria, the mitochondrial targeting sequence is removed to produce a long isoform of Opa1 embedded in the inner membrane. At steady state, about half of Opa1 exists as the long form, with the remainder cleaved to create short forms that are no longer membrane anchored [114]. Under normal conditions, proteolytic cleavage of long isoforms of Opa1 results in the balanced accumulation of long and short Opa1 forms [114, 122]. Long isoforms are crucial for Opa1-mediated mitochondrial fusion while short isoforms are unable to fuse mitochondria [124, 125].
Prohibitins, membrane scaffolds, are required for long Opa1 stability and are essential for the formation and maintenance of mitochondrial structure [126]. A recent study demonstrated a marked increase in Opa1 cleavage by the lack of Prohibitin 2 (Phb2) [121]. Furthermore, overexpression of prohibitin by viral gene transfection protected hippocampal CA1 neurons from cerebral ischemic insult [127].
Two proteolytic cleavage sites in the primary sequence of Opa1 have been identified (S1 and S2) [125]. Two peptidases, m-AAA (mitochondrial matrix oriented) OMA1 and the i-AAA (intermembrane space oriented) protease YME1L, cleave Opa1 at S1 and S2, respectively [116, 125, 128]. YME1L is responsible for cleavage of S2 under basal conditions [115]. OMA1 is activated upon various stress insults, resulting in the complete degradation of long Opa1 and leading to mitochondrial fragmentation [129, 130]. The third protease that was identified to cleave Opa1 was the presenilin-associated rhomboid-like protease (PARL) [125, 131,132,133,134]. The main role of PARL is the generation of a soluble Opa1 isoform detected in the intermembrane space (IMS), which represents 4% of total IMS Opa1 in mitochondria [131, 133]. This soluble Opa1 isoform controls the shape of mitochondrial cristae, independently of its role in mitochondrial fusion.
The focal ischemia model, induced by middle cerebral artery occlusion (MCAO), shows an increase in the long form of Opa1 lasting a few days after insult [49]. Increased Opa1 cleavage and excessive mitochondrial fragmentation was also observed following global cerebral ischemia [53, 56] and neonatal hypoxic-ischemic brain injury [135]. Similarly, there was a cessation of mitochondrial fusion, mitochondrial cristae dilation and reduction of Opa1 levels in a model of excitotoxicity due to increased glutamate release, which is common in stroke, trauma, and neurodegenerative diseases [136]. Overexpressing Opa1 reversed these pathologic changes and increased neuronal survival following excitotoxic stress [136]. As mentioned above, Opa1 activity is also regulated by acetylation and ADP-ribosylation. All these post-translational modifications of Opa1 can ultimately alter mitochondrial morphology and function.
Transcriptional Control
Cells can also alter protein levels via post-transcriptional regulation of gene expression. MicroRNA (miRNA) are small non-coding RNAs that bind to mRNA and silence mRNA usually by initiating its cleavage. Thus, miRNAs are a new class of regulatory RNA molecules whose primary function is to downregulate gene expression. A primary transcript (pri-miRNA) is synthesized from nuclear DNA by RNA polymerase II and then processed by nucleases DROSHA and DGCR8/PASHA. The resulting molecule (pre-miRNA) is transported to the cytoplasm where it is further processed by the nuclease DICER and forms a complex with RNA-inducing silencing complex (RISC) [137]. This complex binds mRNAs by imperfect base pairing and ultimately represses translation [138].
Several miRNAs have been reported to either directly or indirectly regulate mitochondrial fusion and fission proteins. MiRNA-499 regulates calcineurin expression, which is responsible for de-phosphorylation of Drp1. Ischemia/reperfusion (I/R) injury resulted in a downregulation of miRNA-499 which increased calcineurin expression. This resulted in elevated mitochondrial fission due to Drp1 availability at the mitochondrial membrane [139]. MiR-761 targets Mff and is also downregulated in I/R injury, which drives an increase in mitochondrial fission. Delivery of miR-761 rescued fragmented mitochondria in I/R injury [140]. Fis1 expression is directly regulated by miR-484. Anoxic conditions drive increased mitochondrial fission due to decreased miR-484 and elevated Fis1 levels [141]. Fusion protein, Mfn1, is directly targeted by miR-140. Treatment of cardiomyocytes with hydrogen peroxide increases miR-140 levels and reduces Mfn1 expression resulting in fragmented mitochondria [142]. This data suggests that manipulating levels of miRNAs targeting fusion/fission proteins could be used as a therapeutic tool to shift the balance of mitochondrial dynamics in stress or pathological conditions.
Conclusions
Alterations in mitochondrial dynamics are intimately related to mitochondrial function and cellular requirements. Published data suggests the main function of mitochondrial dynamics is to ensure proper inheritance and distribution of mitochondria and to maintain a healthy state. Although the key players regulating mammalian mitochondrial fusion and fission have been identified, their coordination and pathophysiological implications of perturbation in these processes remain poorly understood.
Post-translational protein modifications are important for the spatial and temporal regulation of their function. Under stress or pathologic conditions, several modifications are activated that show complex interactions yielding to a particular functional outcome associated with adaptation of the cellular mitochondrial network to the changing environment (Fig. 4).
A significant or persistent perturbation in the complex regulatory machinery of the mitochondrial dynamics can lead to pathophysiological consequences and cell death. A better understanding of these regulatory mechanisms related to post-translational modifications can likely lead to the development of new therapeutic agents to prevent and treat mitochondria-related neurodegenerative disease. Thus, future studies will need to determine which alterations or combinations of post-translational modifications are significant contributors to pathophysiology of a particular stress or disease. Due to the complexity and synergistic nature of these modifications, it is feasible to assume that several targets will need to be considered for pharmaceutical intervention to achieve a significant therapeutic effect.
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This study was funded by U.S. Veterans Affairs Merit grant BX000917 to TK.
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Nina Klimova, Aaron Long, and Dr. Tibor Kristian declare that they have no conflict of interest.
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Klimova, N., Long, A. & Kristian, T. Significance of Mitochondrial Protein Post-translational Modifications in Pathophysiology of Brain Injury. Transl. Stroke Res. 9, 223–237 (2018). https://doi.org/10.1007/s12975-017-0569-8
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DOI: https://doi.org/10.1007/s12975-017-0569-8