Abstract
Macroporous magnetic agarose particles (MMAPs) were prepared with calcium carbonate as the porogent by the water-in-oil suspension thermal regeneration method. MMAPs with good sphericity and appropriate particle size were obtained. The physical properties of the beads were determined and it was found that the water content (92.1%), porosity (94.4%) and mean pore diameter (120.1 nm) of the MMAPs were higher than those for the normal magnetic particles, indicating successful generation of macropores after calcium carbonate addition. Compared with normal magnetic particles, the mass transfer of biomolecules in MMAPs was remarkably enhanced. Finally, MMAPs were modified with 5-amino-benzimidazol (ABI) ligand and the adsorption capacity of IgG reached 153 mg/mL, higher than that of the normal magnetic particles (126 mg/mL). Moreover, adsorption behavior of MMAPs to IgG was little changed after twenty-five recycled use. Hence, MMAPs prepared herein showed great potential for bioseparation.
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References
Chen, J., Y. Lin, and L. Jia (2015) Preparation of anionic polyelectrolyte modified magnetic nanoparticles for rapid and efficient separation of lysozyme from egg white. J. Chromatogr. A 1388: 43–51.
Zhou, L., Y. Shao, J. Liu, Z. Ye, H. Zhang, J. Ma, Y. Jia, W. Gao, and Y. Li (2014) Preparation and characterization of magnetic porous carbon microspheres for removal of methylene blue by a heterogeneous fenton reaction. ACS Appl. Mater. Inter. 6: 7275–7285.
Santana, S. D., V. L. Dhadge, and A. C. Roque (2012) Dextrancoated magnetic supports modified with a biomimetic ligand for IgG purification. ACS Appl. Mater. Interfaces 4: 5907–5914.
Lin, Z., J. Zheng, Z. Xia, H. Yang, L. Zhang, and G. Chen (2012) One-pot synthesis of phenylboronic acid-functionalized coreshell magnetic nanoparticles for selective enrichment of glycoproteins. RSC Adv. 2: 5062–5065.
Zhao, M., Y. Xie, C. Deng, and X. Zhang (2014) Recent advances in the application of core-shell structured magnetic materials for the separation and enrichment of proteins and peptides. J. Chromatogr. A 1357: 182–193.
Li, D., Y. Zhang, M. Yu, Q. An, J. Guo, J.Q. Lu, and C. Wang (2015) A new strategy for synthesis of porous magnetic supraparticles with excellent biodegradability. Chem. Commun. 51: 1908–1910.
Nazari S. F., P. Hashemi, and F. Rasoolzadeh (2015) A simple method for the preparation of spherical core-shell nanomagnetic agarose particles. Colloid Surf. A Physicochem. Eng. Asp. 465: 47–53.
Fujioka, H., M. Tsunehiro, M. Kawaguchi, Y. Kuramoto, H. Kurosaki, Y. Hieda, E. Kinoshita-Kikuta, E. Kinoshita, and T. Koike (2014) Simple enrichment of thiol-containing biomolecules by using zinc(II)-cyclen-functionalized magnetic beads. J. Sep. Sci. 37: 1601–1609.
Johnson, E. M., D. A. Berk, R. K. Jain, and W. M. Deen (1995) Diffusion and partitioning of proteins in charged agarose gels. Biophys. J. 68: 1561–1568.
Johnson, E. M., D. A. Berk, R. K. Jain, and W. M. Deen (1996) Hindered diffusion in agarose gels: Test of effective medium model. Biophys. J. 70: 1017–1023.
Shi, Q. H., X. Zhou, and Y. Sun (2005) A novel superporous agarose medium for high-speed protein chromatography. Biotechnol. Bioeng. 92: 643–651.
Du, K. F., S. Bai, X. Y. Dong, and Y. Sun (2010) Fabrication of superporous agarose beads for protein adsorption: Effect of CaCO3 granules content. J. Chromatogr. A 1217: 5808–5816.
Xia, H. F., D. Q. Lin, and S. J. Yao (2007) Preparation and characterization of macroporous cellulose-tungsten carbide composite beads for expanded bed applications. J. Chromatogr. A 1175: 55–62.
Levenspiel, O. (1999) Chemical Reaction Engineering. 3rd Ed., pp. 257–277. John Wiley and Sons (WIE), NY, USA.
Gao, D., S. J. Yao, and D. Q. Lin (2008) Preparation and adsorption behavior of a cellulose-based, mixed-mode adsorbent with a benzylamine ligand for expanded bed applications. J. Appl. Polym. Sci. 107: 674–682.
Lu, H. L., D. Q. Lin, D. Gao, and S. J. Yao (2013) Evaluation of immunoglobulin adsorption on the hydrophobic charge-induction resins with different ligand densities and pore sizes. J. Chromatogr. A 1278: 61–68.
Tong, X. D., B. Xue, and Y. Sun (2001) A novel magnetic affinity support for protein adsorption and purification, Biotechnol. Progr. 17: 134–139.
Tong, X. D. and Y. Sun (2001) Agar-based magnetic affinity support for protein adsorption. Biotechnol. Progr. 17:738–743.
Liu, X. Y., S. W. Zheng, R. Y. Hong, Y. Q. Wang, and W. G. Feng (2014) Preparation of magnetic poly(styrene-co-acrylic acid) microspheres with adsorption of protein. Colloid Surf. A Physicochem. Eng. Asp. 443: 425–431.
Wang, D. M., G. Hao, Q. H. Shi, and Y. Sun (2007) Fabrication and characterization of superporous cellulose bead for highspeed protein chromatography. J. Chromatogr. A 1146: 32–40.
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Gu, JL., Tong, HF. & Sun, LY. Preparation and preliminary evaluation of macroporous magnetic agarose particles for bioseparation. Biotechnol Bioproc E 22, 76–82 (2017). https://doi.org/10.1007/s12257-016-0511-z
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DOI: https://doi.org/10.1007/s12257-016-0511-z