Abstract
Genetic variability and relationships among elephant grass cultivars were estimated by the SRAP (sequence-related amplified polymorphism) assay. A total of 60 individuals collected from five cultivars in China were analysed. Sixty-two selected primer combinations generated 1395 bands, with an average of 22.5 per primer combination. The average value of percentage of polymorphic bands (PPB) was 72.8% at species level. The PPB was from 15.2% to 75%, with an average of 39.6% at cultivar level. H POP , within-cultivar Shannon’s index was 1.738 at cultivar level; at species level, the Shannon’s index (H SP ) was 3.880. An assessment of diversity between cultivars [(H SP − H POP )/H SP ] indicated that most of the diversity (55.2%) was detected among cultivars, and only 44.8% was within cultivars in total genetic variation. According to UPGMA dendrogram, the five cultivars were clustered into three main groups. One group included MT-1 and Mott with a bootstrap support of 100%, another consisted of Huanan and N51 with a bootstrap support of 81%, and last one was only Guimu-1. The results indicate that the MT-1 and Mott have a closest genetic relationship; Huanan and N51 possess a relatively close relationship, and Guimu-1 is the most distinct from the other four cultivars.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Budak H., Shearman R. C., Parmaksiz I., Riordan T. P. and Dweikat I. 2004 Comparative analysis of seeded and vegetative biotype buffalograsses based on phylogenetic relationship using ISSRs, SSRs, RAPDs, and SRAPs. Theor. Appl. Genet. 109, 280–288.
Burkill H. M. 1994 The useful plants of West Tropical Africa. Royal Botanic Gardens, Kew, London, UK.
Chen L. W., Gu H. R., Yang Y. S. and Bai S. J. 1991 Studies on response to light and temperature and flowering habits of Pennisetum purpureum. Pratacultural Sci. 8, 19–22.
Chen P., Zhu J., Liu P. and Shi X. L. 2007 Analysis of genetic relationships in new lines of hybrid Pennisetum by RAPD markers. Chin. J. Grassl. 29, 34–38.
Cheng Y. K., Chen C. S. and Wu C. F. 1995 Breeding on yield and chemical composition of dwarf napiergrass. J. Taiwan Lives Res. 28, 285–294.
Chinese herbage cultivar registration board (CHCRB) 2001 Registration of new cultivars of forage. Acta Agrestia Sinica 9, 311–318.
Chinese herbage cultivar registration board (CHCRB) 2002 Registration of new cultivars of forage. Acta Agrestia Sinica 10, 151–156.
Chinese herbage cultivar registration board (CHCRB) 2003 New cultivars of forage and fodder crop. Acta Agrestia Sinica 11, 269–276.
Crozier R. H. 1997 Preserving the information content of species: genetic diversity, phylogeny, and conservation worth. Annu. Rev. Ecol. Syst. 28, 243–268.
Daher R. F., Pereira M. G., Pereira A. V. and Amraral Jr A. T. 2002 Genetic divergence among elephantgrass cultivars assessed by RAPD markers in composite samples. Sci. Agr. 59, 623–627.
Ding A. and Goudet J. 2005 Heterozygote advantage and the maintenance of polymorphism for multilocus traits. Theor. Popul. Biol. 68, 157–166.
Doyle J. and Doyle J. L. 1987 A rapid DNA isolation method for small quantities of fresh tissues. Phytochem. Bull. 19, 11–15.
Frankham R. 2005 Genetics and extinction. Biol. Conserv. 126, 131–140.
Gauer L. and Cavalli-Molina S. 2000 Genetic variation in natural populations of mate (Ilex paraguariensis A.St-Hil., Aquifoliaceae) using RAPD markers. Heredity 84, 647–656.
Hamrick J. L. and Godt M. J. W. 1990 Allozyme diversity in plant species. In Plant population genetics, breeding and genetic resources (ed. A. H. D. Brown, M. T. Clegg, A. L. Kahler and B. S. Weir). pp. 43–63. Sinauer, Sunderland, USA.
Holm L. G., Plucknett D. L., Pancho J. V. and Herberger J. P. 1977 The worlds worst weeds. East-West Center, Honolulu, USA.
Jump A. S., Marchant R. and Peñuelas J. 2009 Environmental change and the option value of genetic diversity. Trends. Plant Sci. 14, 51–58.
Karschon R. and Heth D. 1958 Wind speed, wind-borne salt and agricultural crops as affected by windbreaks. La Yaaran 8, 8–13.
Kumar S., Tamura K. and Nei M. 1993 MEGA: molecular evolutionary genetics analysis. Comput. Appl. Biosci. 10, 189–191.
Li G. and Quiros C. F. 2001 Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor. Appl. Genet. 103, 455–461.
Lombard V., Baril C. P., Dubreuil P., Blouet F. and Zhang D. 2000 Genetic relationships and fingerprinting of rapeseed cultivars by AFLP: consequences for varietal registration. Crop Sci. 40, 1417–1425.
Loveless M. D. and Hamrick J. L. 1984 Ecological determinants of genetic structure in plant populations. Ann. Rev. Ecol. Syst. 15, 65–95.
Mace G. M., Gittleman J. L. and Purvis A. 2003 Preserving the tree of life. Science 300, 1707–1709.
Magcale-Macandog D. B., Predo C. D., Menz K. M. and Calub A. D. 1998 Napier grass strips and livestock: a bioeconomic analysis. Agroforestry Systems 40, 41–58.
Nei M. 1973 Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70, 3321–3323.
Nishimoto R. K. 1994 Weed control in coffee plantations. In Weed management for developing countries. FAO plant production and protection paper 120. (ed. R. Labrada, J. C. Caseley and C. Parker), pp. 354–359. Food and Agriculture Organization of the United Nations, Rome, Italy.
Oostermeijer J. G. B., Luijten S. H. and Den Nijs J. C. M. 2003 Integrating demographic and genetic approaches in plant conservation. Biol. Conserv. 113, 389–398.
Rossetto M., Weaver P. K. and Dixon K. W. 1995 Use of RAPD analysis in devising conservation strategies for the rare and endangered Grevillea scapigera (Proteaceae). Mol. Ecol. 4, 321–329.
Skerman P. J. and Riveros F. 1990 Tropical grasses, FAOplant production and protection series 23. Food and Agriculture Organization of the United Nations, Rome, Italy.
Tinker N. A., Fortin M. G. and Mather D. E. 1993 Random amplified polymorphic DNA and pedigree relationships in spring barley. Theor. Appl. Genet. 85, 976–984.
Wang D., Possw J. A., Donovan T. J., Shannon M. C. and Lesch S. M. 2002 Biophysical properties and biomass production of elephant grass under saline conditions. J. Arid Environ. 52, 447–456.
Wu Y. F. 1999 Volume of forage cultivars registered in China. China Agricultural University Press, Beijing, Peoples Republic of China.
Xie X. M. and Lu X. L. 2005 Analysis of genetic relationships of cultivars in Pennisetum by RAPD markers. Acta Prataculturae Sinica 14, 52–56.
Xu S. B., Tao Y. F., Yang Z. Q. and Chu J. Y. 2002 A simple and rapid method used for silver staining and gel preservation. Hereditas 24, 336–338.
You Y. L., Zhou B. Q., Li B. X., Gan D. J., Dong Z. X. and Lu X. L. 2004 Test of introduced forage varieties of Pennisetum in the south subtropics. J. S. China Agri. Univ. 25, 41–44.
Zhou F., Dong Z. X. and Xie X. M. 2007 Review on the research and utilization of Pennisetum purpureum cv. Mott in tropical and subtropical areas. Grassland and Turf 3, 76–82.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Xie, XM., Zhou, F., Zhang, XQ. et al. Genetic variability and relationship between MT-1 elephant grass and closely related cultivars assessed by SRAP markers. J Genet 88, 281–290 (2009). https://doi.org/10.1007/s12041-009-0041-y
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12041-009-0041-y