Abstract
The prevalence of chronic kidney disease continues to outpace the development of effective treatment strategies. For patients with advanced disease, renal replacement therapies approximate the filtration functions of the kidney at considerable cost and inconvenience, while failing to restore the resorptive and endocrine functions. Allogeneic transplantation remains the only restorative treatment, but donor shortage, surgical morbidity and the need for lifelong immunosuppression significantly limit clinical application. Emerging technologies in the fields of regenerative medicine and tissue engineering strive to address these limitations. We review recent advances in cell-based therapies, primordial allografts, bio-artificial organs and whole-organ bioengineering as they apply to renal regeneration. Collaborative efforts across these fields aim to produce a bioengineered kidney capable of restoring renal function in patients with end-stage disease.
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Introduction
The prevalence of chronic kidney disease (CKD) continues to outpace the development of effective treatment strategies. Best-practice guidelines for CKD suggest supportive measures that minimize comorbidities, but fail to arrest progression. Although tight blood pressure control may help, fewer than 40 % of patients achieve and maintain therapeutic targets [1, 2]. Patients with advanced disease will eventually require life-long renal replacement therapy in the form of peritoneal or haemodialysis. Although lifesaving, dialysis approximates the filtration functions of the kidney at considerable cost and inconvenience, and fails to restore the homoeostatic, resorptive, metabolic, endocrine and immunomodulatory functions. As a result of these shortcomings, patients with CKD remain at an increased risk of cardiovascular and all-cause mortality[3].
Allogeneic transplantation remains the only restorative treatment available for advanced CKD with progression to end stage renal disease (ESRD). Renal transplantation, pioneered by Dr. Joseph Murray [4], is associated with reduced healthcare costs, longer life expectancy and improved quality of life compared to dialysis [5, 6]. The majority of patients are unable to realize these advantages, however, due to the critical shortage of organs that has emerged over the last decades (Fig. 1). Furthermore, the side effects of lifelong immunosuppressive therapy dramatically impact the overall outcome and significantly limit clinical application.
Emerging technologies in the field of regenerative medicine (RM) seek to address the limitations of current treatment strategies. Strategies that are currently being implemented to bioengineer or regenerate kidneys for transplant purposes can be broadly classified into the following categories: (1) cell-based strategies; (2) developmental biology strategies; (3) bio-artificial kidneys strategies; and (4) whole-organ bioengineering strategies.
Cell-Based Strategies
The natural regenerative capacity of the kidney was first demonstrated by Oliver in 1953 using an animal model of acute renal failure [7]. Cuppage and Tate [8] proceeded to identify a population of squamoid cells attached to the basement membrane that were capable of multiplying and migrating to regenerate the tubular epithelium in response to acute injury. These pioneers first recognized that the intact basement membrane plays a crucial role in the regenerative process [7–9]. Renal regeneration after injury requires an up-regulation of gene expression and protein synthesis in response to the local release of inflammatory mediators and growth factors [10]. Up-regulation of C-myc, HSP-70 and urokinase-type plasminogen activator has been demonstrated following mechanical injury of cultured renal cells [11]. Likewise, several cytokines have been shown to influence the rate and efficacy of renal regeneration, including epidermal growth factor [12], hepatocyte growth factor [13], fibroblast growth factor-1 [14], interleukin 1 [15], and TGF-B1 [12, 16].
Cell-based technologies capitalize on these native repair mechanisms by using progenitor and stem cells to induce renal regeneration after injury. Grobstein first demonstrated the presence of renal progenitor cells in the metanephric blastema, a tissue layer of the embryonic kidney [17–20]. Discrete populations of these multipotent renal progenitors have been shown to persist into adulthood in the mesenchyme of the proximal tubule [21, 22], tubular epithelial cells [23], Bowman’s capsule [24], renal papilla [25], and cortical stroma [26]. These cells demonstrate high cloning efficiency and self-renewal potential, suggesting a role in renal regeneration [24]. The transcriptional regulators Six2 [27, 28] and Foxd+1 [26] are essential for the persistence of these progenitor populations beyond development, while Pax2 is required for later differentiation. Bussolati et al. [29] demonstrated that these renal progenitors could be reliably harvested and isolated from adult human kidneys, expanded in vitro, and reimplanted. Intravenously injected renal progenitors were shown to preferentially migrate to the site of tubular injury and contribute to renal repair [29]. The regenerative utility of adult-derived renal progenitors has been similarly demonstrated using cells harvested from Bowman’s capsule [24, 30].
In addition to these resident populations of progenitor cells, extrarenal stem cells can also be induced to differentiate into cells capable of promoting renal recovery. These include embryonic stem cells [31•, 32–38], bone marrow–derived stem cells [39–59], amniotic fluid–derived stem cells [60–63] and induced pluripotent stem cells [64–68].
Embryonic Stem Cells
Embryonic stem cells (ESCs) represent a pluripotent population of cells with the capacity to proliferate in undifferentiated form, while retaining the capacity to develop along ectodermal, mesodermal or endodermal lines [31•], as well as extraembryonic and somatic lineages [37]. The differentiation of ESCs is strongly influenced by elements of the extracellular miroenvironement, including cell–cell contacts, cell–matrix contacts and the paracrine signals [69]. With relation to the kidney, ESCs injected into primordial kidneys develop into primitive renal structures [34, 35, 38], suggesting that nephrogenic factors promote differentiation down renal cell lines. Researchers have attempted to recreate these nephrogenic factors in vitro, and have identified specific growth factors capable of inducing renal differentiation in cultured ESCs [32, 33, 36]. Despite these advantages, the clinical application of ESCs is limited by ethical and legal obstacles, as well as a high potential for teratoma formation [70].
Bone Marrow–Derived Stem Cells
Bone marrow–derived stem cells (BMDCs) allow researchers to circumvent some of the ethical and legal dilemmas inherent to ESC technology. BMDCs, especially mesenchymal stem cells (MSCs), are multipotent, produced throughout life, easily harvested, migrate across tissues, and contribute to the repair of various organs [71]. The administration of BMDCs has been shown to promote neovascularisation, reduce inflammation, inhibit apoptosis, and stimulate differentiation and proliferation in multiple systems [42]. Poulsom et al. first demonstrated that circulating BMDCs engraft into the damaged kidney, promoting the turnover and repair of renal tissues following acute injury [40]. BMDCs have since been shown to contribute to the repair of multiple renal cell types, including tubular epithelia [39, 53, 59], mesangial cells [46, 49, 56, 57], podocytes [51, 52, 56] and endothelial cells [41, 45, 55]. Despite these established benefits, controversy surrounds the mechanism of BMDC-mediated renal repair, as researchers debate whether BMDCs directly repopulate injured nephrons via transdifferentiation, or indirectly through paracrine signalling [43, 44, 48, 54, 58]. Support for the paracrine hypothesis comes from studies showing that BMDC-conditioned medium contains microvesicles and growth factors that reduce inflammation and accelerate renal repair through interactions with renal progenitors [50]. Regardless of the mechanism, injection of exogenous BMDCs has been shown to enhance renal recovery in animal models of acute kidney injury, although a therapeutic benefit in CKD has yet to be demonstrated.
However, the clinical application of BMDCs has been questioned by studies suggesting that these cells may also be involved in kidney disease. BMDCs have been shown to contribute to the development of interstitial fibrosis in mouse models of CKD [47]. Furthermore, direct renal injection of BMDCs has been associated with the development of angiomyeloproliferative lesions of unknown neoplastic potential [72]. Clearly, further research is required before the regenerative utility of BMDCs can be safely and effectively harnessed for clinical applications.
Amniotic Fluid–Derived Stem Cells
Amniotic-derived stem cells are easily harvested and cultured, have low risk for tumour development, and show a breadth of pluripotency similar to BMDCs. Perin et al. demonstrated that human amniotic fluid–derived stem cells (hAFCSs) microinjected into murine embryonic kidneys differentiated along renal cell lines, contributing to the development of primordial kidney structures [60]. Further studies have demonstrated that mTOR plays an essential part in the signalling pathways involved in the renal differentiation of hAFCs [62]. The therapeutic potential of hAFSCs in renal disease has been recently demonstrated by Rota et al. [61], who showed a pro-regenerative effect of hAFSC infusion in an animal model of acute kidney injury. Engrafted hAFSC localised primarily to the peritubular region, improving renal function, limiting tubular damage and prolonging animal survival. Again, the regenerative effect appears to result from a combination of hAFSC transdifferentiation and paracrine signalling including IL-6, VEGF, and SDF-1. The importance of local growth factors and signalling molecules was further demonstrated by experiments showing that hAFSCs preconditioned with GDNF enhanced renal function and tubular repair through increased homing of stem cells to the site of injury. Furthermore, comparative studies have demonstrated that hAFSC treatment normalises renal function more rapidly than BMDCs in an animal model of acute kidney injury [63]. Although BMDCs showed higher potential for proliferation, hAFSCs were more antiapoptotic and persistent within the peritubular capillaries. Importantly, the authors isolated different cytokines and growth factors from BMDCs vs. hAFSCs, suggesting different modes of action.
Induced Pluripotent Stem Cells
Induced pluripotent stem cells (IPCSs) are generated via retroviral infection of somatic cell lines with transcription factors [66]. IPSCs have been successfully generated from human kidney mesangial cells [65] as well as urine-derived renal cells [68]. IPSCs have garnered increasing interest in the field of regenerative medicine, as they may potentially provide an inexhaustible source of patient- and tissue-specific stem cells. Furthermore, the IPSCs have been shown to retain the epigenetic pattern of the parent cell, which may facilitate targeted, organ-specific differentiation [64] with less potential for abnormal tissue formation than ESCs or BMDCs. Despite these advantages, IPSCs have also been shown to induce an immune reaction in syngeneic mice [67], which may compromise the clinical utility. To date, the therapeutic benefit of IPSCs from acute or chronic renal disease has not been demonstrated.
Developmental Biology Strategies
While advances in stem cell technology hold promise for the future of renal regeneration and repair, investigators have further advanced the cellular approach by incorporating principles of developmental biology. This expanding field of renal regenerative medicine endeavours to create, implant and maintain a tissue structure that mimics the physical and physiological characteristics of the native kidney.
Early research in the field sought to add new nephrons to developing kidneys by implanting embryonic metanephric tissue into the renal cortex of neonatal mice [73]. Metanephric tissue is the embryological precursors of the adult kidney, characterised by ureteric buds and mesodermal blastema [74]. Several advantages have been proposed for the transplantation of primitive metanephric tissue over fully developed organs, including reduced immunogenicity due to the absence of native vasculature [74] and antigen-presenting cells [75]. The primitive donor tissue successfully differentiated and developed into functional nephrons in the neonatal host kidney, but these results were not initially reproducible in adult hosts due to lack of differentiation and acute graft rejection [76]. After protocol modifications, Rogers et al. [74] reported the successful engraftment and differentiation of metanephroi into adult hosts, with demonstrated glomerular filtration and plasma clearance. Furthermore, the metanephroi were poorly immunogenic, and were capable of persisting in vivo without host immunosuppression.
Prompted by these early successes, researchers soon endeavoured to expand the technology, investigating the possibility of xenotransplantation and human hosts. The reduced immunogenicity of metanephric transplants could potentially overcome the humoral rejection that has hindered whole-organ xenotransplantation [77–79]. Rogers et al. [80] demonstrated the successful allogeneic transplantation of porcine metanephroi into adult pigs, as well as the xenogeneic transplantation into adult mice. Both types of transplant proved successful, although xenotransplantation required the additional administration of a co-stimulatory blockade including anti-CDR45RB, anti-CD154 and anti-CD11a. Dekel et al. [81] have similarly demonstrated the successful xenotransplantation of human metanephroi into immune-deficient mice, resulting in a pattern of gene expression similar to that seen in human kidney development. Although metanephric xenotransplantation into human hosts has not yet been reported, several potential risks have been hypothesized, including cross-species infection and neoplastic transformation [82].
Researchers seeking to circumvent the limitations of xenotransplantation have sought develop a similarly inexhaustible source of primordial kidney tissue of human origin. Clearly, ethical considerations would prevent the large-scale harvesting of metanephroi from human embryos. Researchers have thus sought a means of culturing and propagating nephrons in vitro, which would allow for the generation of multiple kidney-like tissues from a single metanephroi [83]. Cells derived from both Wolffian duct/ureteric bud and metanephric mesenchyme progenitor tissues have been successfully cultured alone or in co-culture [84•]. Investigators have additionally sought to culture these cell lines on extracellular matrix gels in an attempt to reconstitute essential three-dimensional (3D) relationships [84•]. These efforts have culminated in the successful in vitro construction of renal organoids from single-cell suspensions of embryonic cell lines [85]. Following implantation under the kidney capsule of rodents, these organoids formed vascularized glomeruli and fully differentiated capillary walls that performed physiological functions, including tubular reabsorption. The generation of vascularized nephrons from single-cell suspensions signifies important progress towards the long-term goal of restoring renal function with a bioengineered kidney. However, studies reporting the long-term functiom and viability of these organoids have yet to be published.
Bio-artificial Kidney Strategies
The development of bio-artificial kidneys (BAKs) represents the intersection of regenerative medicine and renal replacement therapy. BAKs combine a hemofilter used in conventional dialysis with a bioreactor unit containing human primary renal proximal tubule cells, termed a renal tubule assisted device (RAD) [86]. The addition of the bioreactor is intended to provide the homoeostatic, resorptive, metabolic, endocrine and immunomodulatory functions of the kidney that are lacking from current renal replacement therapies. Furthermore, these units are designed to be portable or implantable, providing continuous renal support with minimal lifestyle disruption.
Since BAKs were first proposed by Aebischer et al. [87], the technology has matured from concept to clinical trial. Humes et al. reported the safe and efficacious use of BAKs in patients with acute renal failure (ARF) in the ICU setting [88]. The BAK demonstrated metabolic, endocrine and immunomodulatory activities, including glutathione degradation, hydroxylation of calcifediol, and reduction of pro-inflammatory cytokines. Subsequent Phase II trials showed improved 180-day survival in patients with ARF treated with RAD support compared to continuous renal replacement therapy alone [89]. However, these clinical trials have been mired in controversy, and the results have been called into question (see Tasnim et al. [86] for full review).
Whole Organ Bioengineering Strategies
Since Grobstein’s early characterisation of renal organogenesis, researchers have recognized that the extracellular matrix (ECM) plays a crucial role in kidney development and repair [7–9]. The extracellular matrix (ECM) is a three-dimensional (3D) framework of structural and functional proteins in a state of dynamic reciprocity with intracellular cytoskeletal and nuclear elements [90]. ECM molecules and their receptors influence organogenesis and repair by (1) providing a 3D scaffold for the spatial organisation of cells, (2) secreting and storing growth factors and cytokines, and (3) regulating signal transduction [91]. The components of the ECM include type IV collagen, entactin, proteoglycans and the laminins, a family of trimeric glycoproteins that interact with cell-surface integrins. The integrins, especially α8β1, have been shown to be crucial for metanephric proliferation, branching and epithelialization during organogenesis [92]. The importance of the ECM for normal renal function is highlighted by studies suggesting that changes to the ECM underlie common renal pathologies, including diabetic nephropathy [93].
Cutting-edge technologies in RM have recently allowed researchers to exploit and appreciate the advantages of preserving the innate ECM for organ bioengineering investigations [94–97, 98•]. Indeed, innate ECM represents a biochemically, geometrically and spatially ideal platform for such investigations, because it is biocompatible [99•], it has both basic components (proteins and polysaccharides) and matrix-bound growth factors and cytokines preserved, and at physiological levels [100], it retains an intact and patent vasculature, which sustains physiologic blood pressure when implanted in vivo [99•] and is able to drive differentiation of progenitor cells into an organ-specific phenotype [101, 102]. In other words, in natura, innate ECM represents the requisite environment for cell welfare because it contains all indispensable information for growth and function [103]. RM is now exploring the possibility of using intact ECM from animal or human whole organs for bioengineering purposes.
ECM scaffolds from whole animal or human-cadaveric organs can be generated through detergent-based decellularization [95–97, 104] (Fig. 2). Current decellularization protocols are capable of removing DNA, cellular material and cell surface antigens from the ECM scaffold, while preserving attachment sites, structural integrity and vascular channels [105]. Decellularization protocols involve the repeated irrigation of cadaveric tissues with detergents or acids through the innate vasculature, although organs with higher fat content, like the pancreas, often require the addition of lipid solvents, such as alcohol [106]. It remains unclear whether detergent decellularization damages essential components of the ECM, although irrigation through the existing vasculature is thought to limit potentially disruptive ECM exposure. Complete decellularization is essential as residual cellular material may contain antigenic epitopes that trigger inflammatory responses [107] and compromise subsequent recellularization [108]. Following decellularization, ethylene oxide [109] or paracetic acid [110] have been shown to effectively sterilize the ECM without denaturing the ECM proteins or growth factors, although the risk of viral contamination remains [94].
The decellularized, sterilized ECM serves as the scaffold on which stem cells or renal progenitors are seeded with the intent to reconstitute the cellular compartment (recellularization). The successful recellularization of ECM scaffolds has been reported in several organ systems, including liver [111], respiratory tract [112], nerve [106], tendon [113], valve [114], bladder [115] and mammary gland [116]. These results demonstrate the potential of regenerative medicine to dramatically impact organ transplantation, with the possibility of upscaling to more complex, modular organs. Bioengineered organs from autologous cells may enable surgeons to successfully address the two major obstacles of organ transplantation, namely, the need for a new, ideally inexhaustible source of organs, and the achievement of an immunosuppression-free state post-transplantation.
Recent advances have allowed researchers to apply the principles of decellularization-recellularization technology (DRT) to complex organs, including the kidney [99•, 101, 117–119]. Ross et al. [101] were the first to report the successful recellularization of intact rat kidney scaffolds with xenotransplanted murine ESCs perfused through the innate vasculature. ESC cells were chosen as a seeding population due to their high doubling capacity, pluripotency, and potential to differentiate and integrate into primordial kidney cultures. The decellularized kidney scaffold successfully supported the growth and migration of the xenotransplanted ESCs within glomerular, vascular, and tubular structures while inducing differentiation down renal cell lines. Within 10 days of seeding, the ESCs showed gross morphological changes consistent with epithelial maturation, as well as immunohistochemical markers of renal differentiation, including Pax-2 [120], Ksp-cadherin [121] and pan-cytokeratin [122]. Although the importance of their seminal findings cannot be overstated, Ross et al. identified several lines of investigation that must be satisfied in order to advance the field. The authors theorize that pretreatment of the ESC seed population with prodifferentiation agents, such as retinoic acid, activin-A and bone morphogenetic protein 7 (BMP7) [34], may promote engraftment and proliferation by providing a more kidney-specific lineage. Furthermore, although seeding via innate vascular channels provides even cell distribution in the renal cortex, the renal collecting system is omitted. Retrograde seeding through the ureter was attempted to address this problem, but this route was associated with uneven cell dispersion, possibly due to the papillary architecture of the rat kidney. Furthermore, murine scaffolds do not adequately reflect the size and structure of the adult human kidney. The authors suggest that the kidney architecture of higher-order mammals may thus be better suited as a platform for kidney bioengineering. Furthermore, larger pig and primate organs have the physiological and structural capacity to support the critical mass of nephrons required to meet human renal requirements.
The application of DRT protocols to larger, complex organs can be challenging, as perfusion decellularization relies in part on diffusion. Larger organs with greater parenchymal mass require higher perfusion pressures, stronger detergents, and prolonged detergent exposure that may damage the native vasculature and ECM proteins [99•]. Nakayama et al. [118] first performed decellularization studies on sectioned non-human primate kidneys. Microscopy, histology and immunohistochemistry confirmed effective bath decellularization with preservation of native ECM architecture and protein complement. Interestingly, their results showed that decellularization rate correlated inversely with the animal age, due in part to the greater presence of polysaccharide chains in fetal ECM. The authors further performed recellularization via direct extension by culturing scaffolds layered with explants from unrelated donors. Immunohistochemistry showed effective local migration of renal cells to the explant-scaffold border through subsidiary Pax-2 positive and vimentin-positive cell attachment and migration. These findings served as effective proof-of-principle, demonstrating that primate kidneys were amenable to DRT protocols.
More recently, intact, whole-organ ECM scaffolds have been successfully produced from porcine kidneys [99•, 119] (Fig. 3). Orlando et al. [99•] reported effective decellularization of the porcine kidney via detergent perfusion of the innate vasculature. Decellularization was confirmed by histological staining and scanning electron microscopy, which showed total cell clearance and preservation of the scaffolding architecture of the glomeruli, tubules and vessels. Importantly, the decellularization protocol preserved the patency and hierarchical branching structures of the vascular network, which is imperative for subsequent transplant, perfusion and recellularization. The acellular, whole-organ scaffolds were then implanted and surgically reconnected, and maintained for 2 weeks in vivo. Blood pressure was sustained throughout the study, and no blood extravasation was observed, proving the integrity of the intrinsic vasculature. At retrieval, the renal artery and vein were obstructed by massive thrombi despite strong anticoagulation prophylaxis. The observed coagulation was an expected by-product of the interaction of whole blood with the de-endothelialized vascular network. Further dissection showed non-specific inflammatory infiltrate, though the animals did not show any observable signs of fever or infection during the recovery period.
Despite these recent advances, kidney regeneration still lags behind other organs in the field. Although effective protocols have been established for whole-organ kidney decellularization in pigs and rats, similar studies involving non-human primates have not been attempted. Non-human primate studies will become increasingly important as the field advances, as porcine kidneys show marked differences in vascular architecture [123]. Secondly, the attempted recellularization of higher-order mammalian kidneys has not been reported. It thus remains unclear whether larger kidneys can support the retrograde ureteric perfusion needed to recellularize the renal collecting system. These studies are currently underway at our institute. Additionally, the most effective seeding population remains to be determined, although we have reviewed several candidates (see Section 1). Likewise, it is unclear whether the decellularized kidney ECM can support the proliferation and differentiation of stem cells into the approximately 26 requisite cell types that comprise the mature human kidney [124]. Even after reliable recellularization protocols have been established, the homoeostatic, resorptive, metabolic, endocrine and immunomodulatory functions must be re-established by the regenerated kidney. Finally, it is unclear whether perfusion recellularization is sufficient to prevent the thrombogenic effects of the collagen scaffold observed upon re-implantation.
Conclusion
We have reviewed key aspects of RM technologies as they relate to the kidney. Advances in stem cell technology, renal replacement therapy and organ bioengineering and regeneration are intersecting with promise to resolve the dire shortage of transplantable organs. Despite steady progress, renal bioengineering lags behind other organs in the field due to the complex architecture and physiology of the native kidney and to the lack of in-depth knowledge on the interactions between ECM, cells and growth factors. While current research endeavours are promising, the transition to safe and effective clinical implementation faces significant obstacles. Collaborative efforts and investigation are required to drive the field towards the production of a bioengineered kidney capable of restoring renal function in patients with end-stage disease.
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Dr. Marcus Salvatori, Dr. Andrea Peloso, Dr. Ravi Katari, and Dr. Giuseppe Orlando reported no potential conflicts of interest relevant to this article.
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This article does not contain any studies with human or animal subjects performed by any of the authors.
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Salvatori, M., Peloso, A., Katari, R. et al. Regeneration and Bioengineering of the Kidney: Current Status and Future Challenges. Curr Urol Rep 15, 379 (2014). https://doi.org/10.1007/s11934-013-0379-9
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DOI: https://doi.org/10.1007/s11934-013-0379-9
Keywords
- CKD Chronic Kidney Disease
- ESRD End-Stage Renal Disease
- RM Regenerative Medicine
- ESC Embryonic Stem Cell
- BMDC Bone Marrow-Derived Stem Cell
- MSC Mesenchymal Stem Cell
- Hafsc Human Amniotic Fluid-Derived Stem Cells
- IPSC Induced Pluripotent Stem Cells
- BAK Bio-Artificial Kidney
- RAD Renal Tubule Assisted Device
- ARF Acute Renal Failure
- ECM Extracellular Matrix
- DRT Decellularization-Recellularization Technology