Abstract
A diatom was purified with colony selection and continuous dilution methods. It was identified to Cylindrotheca closterium according to its morphological characteristics and rbcL and 18s rRNA gene sequences. The alga was not sensitive to ampicillin and neomycin, but sensitive to chloramphenicol which inhibited its growth at concentrations ranging from 50 to 150 μg mL−1. The purified alga was easy to culture and its specific growth rate was 0.207 ± 0.002 (d−1). It was resistant to pollution and could be harvested in an easy way. It was relatively high in lipid content (20.08% ± 0.67% of dry weight) and the combined amount of its 16:0 and 16:1 (n-7), the most suitable resource of biodiesel, was as high as 64% of the total fatty acids, while the amount of polyunsaturated fatty acids reached 19.96%–20% of the total fatty acids. Thus the purified C. closterium can be cultured as a biodiesel producer or a nutrition supplement producer.
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Wang, S., Zhang, L., Yang, G. et al. Purification of a diatom and its identification to Cylindrotheca closterium . J. Ocean Univ. China 14, 357–361 (2015). https://doi.org/10.1007/s11802-015-2404-8
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DOI: https://doi.org/10.1007/s11802-015-2404-8