Abstract
Poxvirus epidemics occur almost every year and cause significant economic losses for small-scale animal producers in Turkey. In this study, the causative agent of the most recent epidemic in Central Anatolia was detected in clinical samples using electron microscopy (EM) and amplified using an in house polymerase chain reaction procedure for the first time. Additionally, the aetiological agent was isolated from a sheep and identified using EM and PCR.
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Abbreviations
- AGID:
-
agar gel immunodiffussion
- CPE:
-
cytopathic effect
- DIFA:
-
direct immunofluorescence assay
- ELISA:
-
enzyme-linked immunosorbent assay
- EM:
-
electron microscopy
- HE:
-
haematoxylin–eosin
- OIE:
-
Office International des Epizooties
- PBS:
-
phosphate-buffered aliae
- PCR:
-
polymerase chain reaction
- PTA:
-
phosphotungstic acid
- SFT-R:
-
sheep fetal thymus cells
- TCID50 :
-
tissue culture infective dose 50%
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Oğuzoğlu, T.Ç., Alkan, F., Özkul, A., Atalay Vural, S., Güngör, A.B. and Burgu, İ., 2006. A sheeppox virus outbreak in Central Turkey in 2003: Isolation and identification of capripoxvirus ovis. Veterinary Research Communications, 30(8), 965–971
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Oğuzoğlu, T.Ç., Alkan, F., Özkul, A. et al. A Sheeppox Virus Outbreak in Central Turkey in 2003: Isolation and Identification of Capripoxvirus Ovis. Vet Res Commun 30, 965–971 (2006). https://doi.org/10.1007/s11259-006-3259-7
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DOI: https://doi.org/10.1007/s11259-006-3259-7