Abstract
Aims
The Ilam Gas Refinery (IGR) emitting atmospheric trace elements affect soils and Baneh trees (Pistacia atlantica Desf.) of semi-arid Zagros forests. Therefore, the seasonal concentrations of Pb, As, Cu, Cr, Mn, Zn, Ni, and S were analyzed in soils, barks, and leaves of trees located at various distances (1000, 1500, 2000, 2500, and 10,000 m (control) from the refinery.
Methods
The seasonal changes, source distance, soil physicochemical properties (i.e., pH, electrolyte conductivity (EC), and texture), and organ-level atmospheric element deposition were detected in the study.
Results
The season × distance effects on the soils, barks, and leaves revealed that the summer and autumn seasons and distances up to 2500 m from the IGR had higher element concentrations than spring and control distance. The total element trends were in decreasing order; soil > leaf > bark. High pollution was defined as soils with a pollution load index (PLI) of 5.3, with sulfur having the highest concentration. Leaves and barks with metal accumulation index (MAI) of 12.51 and 11.18, enriched by elements in particular with S and Cu.
Conclusions
The Baneh trees might be ideal for atmospheric element remediation and be employed among practical techniques for reducing IGR pollution in Zagros forests. In addition, since the organisms that feed on these trees might be a hub for pollution dispersion across the food web, it is recommended that pollutant levels in the aerial organs and soils in the region be monitored regularly.
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Introduction
Anthropogenic factors (e.g., industrial operations) and natural activities are one of the primary sources of trace elements (TEs) enrichment and contamination (Lee et al. [20]; Li et al. [23]). Low concentrations of TEs are long-lasting and indestructible in a variety of environments where plants and humans have been exposed (Milicevic et al. [28]; Cao et al. [8]). The higher TE concentrations may negatively affect the air and soil quality, aquatic or terrestrial habitats, and food chain. Therefore, TEs have gained significant attention in recent years (Li et al. [23]).
Sulfur is a macronutrient needed for plant growth, metabolism, and environmental stress response. Sulfate, sulfur dioxide, and hydrogen sulfide are the forms in which sulfur could be accumulated in soils and leaves, respectively (Leustek and Saito [22]; Mutia et al. [30]). However, sulfur accumulation is controlled by the number of different forms deposited in the environment, and rising quantities would not help sensitive terrestrial ecosystem components (Kravitz et al. [19]).
Industry's impact on pollution and the risk of TEs accumulation in the ecosystems has grown in recent years (Ramachandra et al. [35]). Several methods are widely used to evaluate TE concentrations in soils, such as enrichment factor (EF), geo-accumulation index (Igeo), pollution load index (PLI), and potential ecological risk index (RI; Liu et al. [26]). The bioaccumulation factor (BF) is also a pollution indicator in ecosystems that measures plants' tendency to take and translocate TEs from soils into their biological systems (Baker [5]).
There is a scarcity of studies on the potentially TEs contamination in forest soils vs. urban and agricultural soils (Turtscher et al. [43]). So far, only a few studies have been performed on biomonitoring of TEs in ecosystems components such as soils, trees, and their various organs in the areas near the gas refinery and petrochemical plants in Iran (Safari et al. [38]). Leitgeb et al. ([21]) evaluated different soil properties, S, and potentially TEs (As, Mn, Cu, Cr, Ni, Pb, and Zn) concentrations in forest soils of Singapore and discovered that anthropogenic activities had little impact on soils.
Baneh trees, also known as wild pistachio trees (Pistacia atlantica subsp. Mutica), are the second indigenous deciduous tree species in Iran's Zagros woodlands, where they coexist irregularly with the various oak trees. The indigenous people of Zagros get multiple benefits from using different components of Baneh trees in agriculture, trade, and industry. However, in addition to living on drought stress conditions in semi-arid Zagros climate (Sheikh-Hassani et al. [40]), these trees are confronted with another lousy situation due to the aforementioned (i.e., anthropogenic, natural) activities. Air pollution emitted from the industrial stationery resources, such as Ilam Gas Refinery (IGR) in Ilam province forests, is a safety issue addressed in this research. The IGR source, emitting toxic gaseous pollutants from the two different chimneys, affects the Zagros ecosystem, including the soils and tree species (e.g., pistachio).
In Iran, the medicinal and therapeutic qualities of Baneh trees have been examined in various studies. However, little analysis has been performed on the impacts of environmental and abiotic stress on the Zagros forests ecosystem, such as air pollution from industrial sources. In this context, we look at the concentrations of sulfur and other TEs in Baneh trees due to refinery pollution and the impacts of pollution on tree leaves, barks, and soil samples collected at different periods of the year with varying distances from the pollution source. The toxic concentration of elements is critical for animals that feed on these plants and people using the trees for pharmaceutical purposes. For instance, cattle, sheep, and goats that feed on Baneh trees in this particular air polluted region may be susceptible to Baneh poisoning. Besides, the toxicity of elements such as sulfur can cause injury symptoms, including necrosis and colored spots in the leaf organs. To the best of our knowledge, this is the first study on the effects of air pollution on Baneh trees in Iran's Zagros region.
The objectives of this research were (i) to investigate the seasonal and distance effects of Ilam Gas Refinery on the concentration of sulfur and TEs (ii) quantification and assessment of various pollution indices of elements in soil samples, barks, and leaves of Baneh trees (iii) analyze the relationship between sulfur and different TEs through principal component analysis (PCA) in soils and Baneh tree components which affected by the gas refinery (iv) provide a perspective and a basis to assess the effects of polluted soils and trees on the food chain in an ongoing process. We hypothesize that: (i) the concentration of S and TEs in the soil samples and Baneh tree components will be affected by seasonal and distance changes from the gas refinery (ii) barks and leaves of trees will react in different ways to the pollution (iii) sulfur and different TEs in tree components mainly accumulated from the atmospheric portions.
Material and methods
Description of the study area
The Ilam Gas Refinery (330 42/ N, 460 13/ E), which is operating gas conversion, is located along the edge of the mountains of Zagros forests. The refinery was founded in 2007 and primarily supplies gas to Iran's western provinces with its two chimneys, which release waste gases into the atmosphere. Based on random analysis of exhaust emissions, sulfur dioxide and hydrogen sulfide concentrations were detected, the highest among the other pollutants emitted from the refinery (Aghdash et al. [1]). In addition, Baneh trees in Zagros forests around the refinery are exposed to various air contaminants (different elements) from this source. The sampling points have an average altitude of about 1000–1200 m and less than 25% slope. The climate of the region is semi-arid and influenced by Mediterranean winds. The predominant wind direction is west and southwest (http://www.ilammet.ir).
Sample collection and analysis of different elements in soils and tree components
Different distances (1,000, 1,500, 2,000, 2,500, and 10,000 m [control]) in an easterly direction from the facility (Fig. 1) was studied. Based on different articles on SO2 pollution sources (Cicek and Koparal [9]; Mutia et al. [30]) and the Iranian environmental science reports (modeling, predicting, and assimilating air pollutants from various sources), 10 km from the gas refinery was selected as the control distance. In the late spring, summer, and autumn seasons of 2019, soils, barks, and leaves were sampled at each distance. Because of more prevalent spring rains, summer was hotter and drier, and autumn led to leaf senescence and wind velocity. In this research, seasonal changes' effects on element accumulations in the soil and trees were assayed.
Two soil samples were obtained with a plastic gardening shovel from a depth of 0–20 cm at each distance, and roughly 25 g of healthy barks were sampled by cutter from the middle of the tree trunk and at about 100 cm above the ground. After the bark sampling, the Baneh trees with special adhesives were protected from microorganism infection. Twenty healthy and mature leaves were taken from a particular height (around the middle of the canopy) of the outer canopy of two Baneh trees. The trees with an average height of 4 m and a diameter of 35 cm at breast height were chosen randomly.
The samples were placed into polyethylene bags and transported to the lab. for analysis (Estefan et al. [12]). The soil, bark, and leaf samples were dried for ten days until they reached a constant weight at lab temperature. The leaves were then grounded, soils were sieved with ASTM mesh with a diameter of 2 mm and homogenized. To determine the pH and electrical conductivity (EC) of soils samples, 2 g of the soil samples were shaken in 10 ml of ddH2O with a ratio of 1:5. After an hour, the pH and electrical conductivity (EC) of the solution were measured by a digital pH (Fan Azma Gostar Company, Iran) and EC meter (Sartorius, PT-20, USA; Pen-Mouratov et al. [33]). The soil particle sizes were analyzed using the hydrometer method, and texture class was determined with a soil texture triangle (Palmer and Troeh [32]).
The soil and leaf samples were prepared and digested according to different U.S.EPA protocols modified by the following references. In the soil, leaf, and bark samples, the concentration of sulfur (S), arsenic (As), chromium (Cr), copper (Cu), lead (Pb), zinc (Zn), manganese (Mn), and nickel (Ni) were measured via inductively coupled plasma mass spectrometry (ICP-MS, 7500 CS, Agilent, US).
According to Da Silva et al. ([10]), approximately 0.5 g of dry soil sample was digested with 10 mL of HCl on a hot plate at ~ 180 °C until the solution was reduced to 3 ml. Approximately 5 mL of HF (40%, w/w), 5 mL of HNO3 (63%, w/w), and 3 mL of HClO4 (70%, w/w) were then added, and the solution was digested. This process was continued by adding 3 mL of HNO3, 3 mL of HF, and 1 mL of HClO4 until the silicate minerals disappeared. This solution was transferred to a 25 mL volumetric tube, and 1% HNO3 was added to bring the samples up to a constant volume. After filtering the digested samples, the concentrations of studied elements were measured via an ICP-MS device (7500 CS, Agilent, US). The procedures of quality assurance and quality control (QA/QC) were performed. External standards with calibration levels were used to quantify element contents from soil samples. The precision and the repeatability of the analysis were tested on the instrument by analysing three replicate samples.
According to Liang et al. ([24]), 0.5 g of dry bark and leaf samples were acid digested, sieved powder samples were placed in the acid-washed tubes, and 10 mL of 65% nitric acid was added. The solutions were placed at room temperature overnight (12 h) and 100 ℃ for an initial 4 h and then another 4 h at 140 ℃ until the solutions` color was clear. After cooling, the solutions were diluted by deionized water to 50 mL and then passed through Whatman filter paper until 25 mL of the filtrate volume was obtained. Each sample was digested three times, and the average measurements were recorded. Total elements were measured by using the ICP-MS. A control sample was also used beside each sample to determine the background contamination during digestion. To confirm the methodology's accuracy and ensure the extraction of TEs, the standard solution of each studied element was used.
Pollution range of different elements in soils and tree components
For assessment of contamination levels (concentrations) of different elements in soils and trees, standard indices of pollution including geoaccumulation index (Igeo), pollution indices (PI), pollution load indices (PLI), enrichment factor of plants (EFplant), bioconcentration factor (BCF) and metal accumulation index (MAI) were used.
Igeo was calculated using the following equation:
where Cn is the measured concentration of the element n, Bn is the geoaccumulation background for this element, and 1.5 is a constant coefficient used to eliminate potential variations in the baseline data (Solgi et al. [41]). The Igeo classifies samples into seven grades: < 0 for practically unpolluted; 0–1 for unpolluted to moderately polluted; 1–2 for moderately polluted; 2–3 for moderately to strongly polluted; 3–4 for strongly polluted; 4–5 for strongly to extremely polluted; and > 5 for extremely polluted (Qing et al. [34]).
The first PI is expressed as Eq. 2:
where Ci is the concentration of element i in the soil (mg kg−1) and Si is the soil quality standard or reference value for the element i (mg kg−1). The PLI for different elements is calculated via the following equation:
The PLI of soils is classified as follows: PLI < 1 is unpolluted, 1 < PLI < 2 is unpolluted to moderately polluted, 2 < PLI < 3 is moderately polluted, 3 < PLI < 4 is moderately to highly polluted, 4 < PLI < 5 is highly polluted, and PLI > 5 is very highly polluted.
Plant EF is calculated as:
where Cplant and Ccontrol are element concentrations (mg kg−1) in tree leaves or barks at the polluted and the control sites, respectively. A value of EF > 2 indicates that the tree is enriched with a specific element (Mingorance et al. [29]). BCF, which indicates the ability of plants to accumulate different elements from the soil (Alahabadi et al. [2]), is computed as follows:
where [C]LB and [C]S are the concentration of different elements in leaf and bark and soil samples, respectively. Values of BCF > 1, BCF < 1, and BCF = 1 imply that the trees are accumulators, excluders, and indicators for different elements, respectively (Liang et al. [24]).
The ability of Baneh tree leaves and bark to accumulate atmospheric elements differed. In this context, MAI was calculated as follows:
where n is the total number of studied elements, and Ij is the sub-index of variable j, calculated by dividing each element's mean concentration (x) by its standard deviation (Liu et al. [25]; Hu et al. [13]).
Data analysis
The normality of the data distribution was checked by SAS software using Shapiro-Walk and Kolmograph-Smirnov tests before employing analysis of variance. The logarithmic conversion was used to normalize the data. The concentration of Pb, As, Cu, Cr, Mn, Ni, Zn, and S in soils, barks, and leaves of Baneh trees along with pH and EC in soils for two factors including seasons in three levels (spring, summer, and autumn) and distance in five levels (1,000, 1,500, 2,000, 2,500, 10,000) were analyzed with three replicates in a completely randomized design. Two-way ANOVA was performed, and the comparisons of means were conducted with Duncan’s test at p < 0.05. Principal components analyses for different elements were performed by SAS software.
Results
The seasonal and distance effects of IGR on different elements concentration of soil samples and Baneh tree components
Season × distance effects on pH and EC of soil samples revealed no significant (p > 0.05) differences between spring and autumn seasons at various distances from the IGR. Soils in summer at a distance of 1000 had the lowest pH (8.32) among the studied distances (Fig. 2a). While soil samples in spring at 1500 and 2000 m had the highest EC with 0.39 and 0.13 ds.m−1, the highest EC (with 0.32 and 0.25 ds.m−1) in summer was 1000 m and 10 km. Soil sampled from 1500 m showed the lowest EC among the other distances tested (Fig. 2b). The soils exhibited a loam clay texture at 1000 and 1500 m and a loam texture at other distances. Clay, silt, and sand percentages were 40.6%, 25%, and 34.4% respectively, at various distances from the IGR.
Season × distance effects on different elements concentration of soil samples revealed that Cr, Cu, Mn, Ni, Pb, and Zn elements had a substantially greater concentration in different seasons, at distances up to 2500 m from the IGR, than control distance soils (p < 0.05; Table 1).
Soil samples in spring at distances of 1000 and 1500 m showed lower Cr, Cu, Mn, Ni, Zn, and Pb concentrations than 2000 and 2500 m from the pollution source. Compared to 1500, 2000, and 2500 m distances from the source, soils at 1000 m had the lowest Cr, Cu, Mn, Ni, Zn, and Pb concentrations during the summer and autumn seasons. The amount of arsenic (As) in soil samples collected during the spring and summer seasons at 2500 m distance from the IGR was not significantly different from control distance soils. However, soil samples in the autumn season at different distances (1000, 1500, 2000, and 2500 m) showed higher As concentrations than control soils (Table 1).
S concentrations in soils were significantly higher (p < 0.05) in different seasons at distances up to 2500 m from the IGR than the control distance. Furthermore, the highest S concentrations were found in soils at distances of 1000, 1500, and 2000 m, respectively (Fig. 3a).
The season × distance effects on different element concentrations of bark samples indicated that Cr in spring and autumn had no significance among the investigated distances from the IGR. Also, barks sampled up to 2500 m showed higher Cr than control distance. Cu concentrations were considerably higher (p < 0.05) in the spring and autumn seasons at distances up to 2500 m from the source than in control distance barks. Also, 2500 and 10,000 m from the pollution source had lower Cu than other distances in the summer. Mn, and As in different seasons, was found in significantly (p < 0.05) higher concentrations than control distance barks sampled up to 2500 m from the IGR. In the spring and autumn, there was no significant difference in Ni concentration; however, in the summer, the concentration was lower at the control distance than at the other distances. Compared to control, the Zn content of barks in three seasons at varied distances had the highest concentration. Barks in spring and summer with 13.35 and 9.33 mg.kg−1 at 1000 m and in autumn with 16.33 mg.kg−1 at 2500 m showed the highest Pb concentrations (Table 2).
S concentrations were significantly higher (p < 0.05) in different seasons at distances up to 2500 m from the IGR than in control distance barks. The barks showed the highest S concentrations in spring and summer at 2000 m and had the lowest concentrations in 2500 m and control distances (Fig. 3b). S element in different seasons at distances up to 2500 m from the IGR, significantly (p < 0.05) had higher concentration than control distance leaves. There was no discernible variation between seasons at a distance of 2500 m from the source (Fig. 3c).
The results of the season × distance effects on the concentration of different elements in leaf samples revealed that Cr element had significantly higher (p < 0.05) concentration than control distance tree leaves in the spring and summer seasons at distances up to 2500 m from the IGR. Also, at 1500 m from the source, the Cr concentration was the lowest compared to other distances.
Cu, Pb, Zn, and As concentrations were significantly higher (p < 0.05) than control distance leaf samples in different seasons at distances up to 2500 m from the source. There was no significant change in Mn concentrations between leaf samples collected in spring and fall at distances of 1500 and 2500 m compared to other distances. In comparison to the control distance, Mn concentrations were higher at various distances. Leaf samples in spring and autumn at distances of 2500 m had no significant difference in Ni concentration compared to other distances with control. However, there was a higher Ni concentration in different distances than the control distance (Table 3).
Multivariate analysis of different elements in soil samples and tree components
The results of principal components analysis (PCA) in the soil samples showed that the first and second main components with Eigenvalues of 6.09 and 1.61, respectively, explained a total of 77.09% of the total variance of the components (the first component equals 60.94% and the second component was 16.15%). The first component has a positive and significant correlation with the elements of Ni (0.97), Cu (0.96), Mn (0.95), Cr (0.94), Pb (0.93), and Zn (0.92). The second component, which represents soil properties, includes 16.15% of the variance, which was positively and negatively correlated with the EC (0.87) and pH (-0.60), respectively. This component is thought to reflect soils with high salt and low acidity (Table 4).
In the bark samples, the first and second components had Eigenvalues of 4.69 and 1.19, respectively, and accounted for 73.58% of the total variance (the first component was 58.69%, and the second component was 14.88%). The PCA results for bark showed a high and positive correlation for the first component of Pb, Zn, Ni, As, and Cr. In addition, a positive correlation was observed based on the second component of Cu and S elements. According to the PCA results for the leaf samples, all elements except Cr had a positive correlation with the first component (Table 4).
In all three seasons, the control distance had the lowest concentration of TEs in the soil. The highest amount of soil EC was observed in the summer and autumn seasons near the IGR. Regardless of distance from the refinery, soils in spring showed the lowest acidity and salinity. The highest concentrations of Pb and S were observed in autumn at closer distances to the refinery (2000 m). In addition, the soils at various distances from the refinery (1500, 2000, and 2500 m) exhibited significant amounts of Zn, Cu, Cr, Mn, and Ni in the summer and autumn seasons (Fig. 4).
According to the PCA, the control distance had the lowest TE concentrations in the bark samples in all three seasons. The maximum concentrations of Pb, As, Zn, Ni, and Cr were found at a distance of 1000 m from the IGR in the spring, while the lowest concentrations of Cu, S, and Mn elements were found at a distance of 1000 m. This distance showed the highest Pb, As, Zn, Ni, and Cr concentrations in summer and autumn. The concentrations of Cr, S, and Mn were not affected by the season and were highest at 1500 and 2000 m. The lowest concentration of these elements was observed at 2500 m from the IGR (Fig. 5).
Pollution assessment of elements in soils and tree components
The mean element concentrations (mg kg−1) of Cr, Cu, Mn, Ni, S, and As were higher than specified in the soil quality standards of the Department of Environment of Iran. Also, Ni, Cr, Pb, S, and As were detected higher than the world soil standards (Cicek and Koparal [9]; Alloway [4]). The mean concentrations of the elements examined in Baneh bark samples were As > S > Mn > Cu > Pb > Zn > Ni > Cr in decreasing order. The mean element concentrations in leaves were 446.19 for S, 19.75 for Mn, 17.18 for Zn, 9.27 for As, 6.41 for Cu, 5.63 for Ni, 4.23 for Cr, and 3.67 for Pb; (S > Mn > Zn > As > Cu > Ni > Cr > Pb) (Table 5).
The different pollution metrics, including Igeo, PI, PLI, BCF, EF, and MAI, were calculated for various elements in the soil, bark, and leaf samples across different seasons and distances around the Ilam gas refinery. The mean PI and Igeo values for S, Cr, Cu, Mn, Ni, Pb, As, and Zn were S > As > Pb > Mn > Ni > Cu > Zn > Cr. S and Zn elements were found to have the highest and lowest pollution levels in soil samples, respectively. The mean PLI in all soils was 5.09 (Table 6). The mean EF values of leaf samples for S, Cr, Cu, Mn, Ni, Pb, As, and Zn, was S > As > Pb > Cr > Cu > Ni > Zn > Mn in decreasing order. The mean EF values of bark samples for S, Cr, Cu, Mn, Ni, Pb, As, and Zn, was Pb > As > Zn > S > Mn > Cu > Ni > Cr (Table 6).
The leaf samples had mean BCF values of 0.36 for S, 0.08 for Cr, 0.29 for Cu, 0.06 for Mn, 0.07 for Ni, 0.94 for Pb, 0.19 for As, and 0.40 for Zn: Pb > Zn > S > Cu > As > Cr > Ni > Mn. The mean BCF values of bark samples were 0.02 for S, 0.03 for Cr, 0.36 for Cu, 0.07 for Mn, 0.03 for Ni,1.65 for Pb, 1.23 for As and 0.10 for Zn; thus, as follows: Pb > As > Cu > Cu > Zn > Mn > Cr = Ni > S (Table 6). The findings of the mean metal accumulation sub-index and MAI of the elements examined in tree leaves and barks revealed that the concentrations were in decreasing order, S > Cu > Zn > Mn > Cr > As > Pb > Ni and Cu > Mn > As > S > Pb > Zn > Ni > Cr respectively. The MAI of leaves and barks were 12.51 and 11.18, respectively (Fig. 6).
Discussion
According to our findings, environmental variables such as soil characteristics, seasons of the year, and distances from the pollution source impacted the concentration of sulfur and TEs in soil, bark, and leaf samples of Baneh trees in Zagros forests. Also, the primary variables for pollutants transport and deposition in Zagros forest components were the height of the IGR flare gas exhaust tower and wind velocity.
Clay particles in soil samples exhibit the most remarkable ability for retaining sulfur and other elements in their structure (Rodriguez et al. [37]). Although the pH of the soils varied depending on the season and distance, they were all characterized by an alkaline state (pH > 8.3). The presence of carbonate sodium, the entrance of calcareous matters, parent material rocks, and anthropogenic pollution sources might be the primary reasons soils were in alkaline condition (Solgi et al. [42]). However, the soil samples having an EC of < 1 were not salty in any way. These characteristics resulted in soils with low conductivity for the elements examined.
Other studies have shown that our findings follow a similar pattern: in an assay of soils sampled from several industrial locations in Ahvaz (western Iran), Keshavarzi et al. ([17]) discovered that soils had an alkaline condition, low EC, and virtual clay fraction characteristics. In another work, the predominant texture of soils was loam, according to heavy metal monitoring in various soils of Bojnord, Iran. Furthermore, the soils were not saline (low EC) and had a neutral to alkaline pH range (Solgi et al. [42]). In essence, the significant variables that caused soils to have a higher ability to accumulate TEs were loam texture, alkaline pH, and low EC.
Soil samples from the furthest distances (1500, 2000, and 2500 m) exhibited greater sulfur and TEs contents than samples from the closest distance. Furthermore, soils sampled up to 2500 m from the IGR showed higher element concentrations than soils at a control distance. This refinery's exhaust output properties, such as size or height of the exhaust tower and output loads per unit of time, are the crucial factors to emit air pollution to surrounding ecosystem components. Certain studies have confirmed this data. For instance, Mutia et al.([30]) found that in Kenya, soils close to a pollution source (1000 m near) showed lower sulfur concentrations than soils sampled from a larger radius. Thus, the usual pattern was found in this study.
In terms of seasonal impacts on element concentrations in soils, in comparison to other seasons, the humidity and dispersed rainfall of the Zagros forests in spring cause significant amounts of air pollutants and elements to be washed and deposited in soils. However, due to the dryness of the air in subsequent seasons, pollutants accumulated into the soils at high concentrations. As a result, soils in the summer and autumn were subjected to drought stress or a lack of moisture in addition to air pollution. These circumstances resulted in increased element concentrations in the soils throughout these seasons.
Both seasons and distances from the IGR impacted S and TEs concentrations in tree barks and leaves. Furthermore, the concentration of studied elements at distances up to 2500 m was higher than that of the control distance. In line with our results, another work to analyze the impact of a pollution source on ecosystems in Turkey revealed that the concentrations of sulfur and TEs in tree leaves at the furthest distance (10 km) were the lowest among the other distances from the source (Cicek and Koparal [9]). However, due to dry weather and arid lands of these ecosystems, and a lack of rainy seasons, significant pollution accumulated in tree organs throughout the summer and autumn seasons compared to spring (Richardson et al. [36]).
Based on the PCA results, there were significant positive correlations between TEs in soil, bark, and leaves of trees directly impacted by the IGR's air pollution. The concentration of the elements tested was found proportional to their distance from the gas refinery. As a result, their quantities near the source (1500 to 2000 m) exceeded the control distance. The concentrations of Zn, Cu, Cr, Mn, and Ni were higher in barks located at different distances (1500, 2000, and 2500 m) from the source. Rather than the size of emitted particles. e.g., dust and smoke particles that carry TEs are essential factors in the differences of air pollution amounts in the nearest and farthest distances from the gas refinery.
In line with our results, various elements had a positive correlation in each portion of soils, barks, and leaves of trees, and all of them may have originated from IGR operations. De Nicola et al. ([11]), in their study of TEs monitoring of soils and oak trees (Quercus ilex L.) growing in different locations, concluded that Mn and Cu concentrations of soil and leaves were correlated that it might indicate the same source of origin. Our data showed that the concentration of S and TEs in summer and autumn was higher than spring season. In the summer and autumn seasons, the concentration of TEs and S in leaves was higher also because the time of TEs accumulation was more prolonged than in spring when leaves appear on the trees and the trees just start to uptake water with elements more intensively after winter. Baneh trees at distances growing in 1000-2500 m from IGR were affected by the pollution, mainly As toxicity (morphological differences were such smaller leaves and necrosis in leaves) compared to control distance trees.
The mean concentration of S (2.85 times), As (29.63 times), Cr (1.12 times), and Ni 3.46 times were higher than the World healthy soil requirements (Cicek and Koparal [9]; Alloway [4]). Thus, As element had the highest toxic range among the other studied elements in the Zagros region soil samples, this is the area where the most detrimental TEs contamination for soil and plants occurred, depending on the season of the year. Furthermore, in line with our results that sulfur concentration in soils and leaves were found more elevated than that of the regulated limits, in the work of Cicek and Koparal ([9]), sulfur in soils and oak trees (Quercus infectoria L.) living adjacent to an SO2 pollution source in Turkey showed higher concentrations than that of the regular range for soils and plants.
According to Kabata-Pendias and Pendias ([14]); Padmavathiamma and Li ([31]), the concentrations of Cu, Zn, and Pb in plants are in the ranges of 2–20,10–150 mg kg−1, and less than 10 mg kg−1 are normal. In our results, these elements were below the regulated limits in Baneh trees. Safari et al. ([38]) showed that the concentration of Pb element contrary to Ni had the toxic range in the leaf and bark of deciduous tree species at different distances from the gas refinery. However, in our research, Pb and Ni elements did not exceed the limits in barks and leaves.
The mean Igeo of As and S were in moderately to strongly polluted ranges, and Pb, Mn, and Ni were in moderately polluted ranges. Cr, Cu, and Zn Mn were unpolluted to moderately polluted ranges. Although sulfur and As had a high PI, Cr, Zn, and Cu had relatively low PI indices. The PLIs in all soil samples were in a very highly polluted range. The IGR's operations contaminated soil samples from various seasons and distances. All of the investigated TEs were enriched in the leaves and barks of Baneh trees (with EF > 2). Also, the leaves showed higher EF than the barks of trees. This elemental enrichment might come from the soil or the atmosphere.
The BCF demonstrated the capacity of various organs of trees to accumulate TEs from the soil. To all of the elements tested, tree leaves had a BCF < 1. However, tree barks had BCF > 1 for As and Pb elements, showing that Baneh trees could absorb these from soil readily. The concentration of As element was high in the bark. According to our findings, it was deposited from the atmospheric section and absorbed from the soil. Besides, this is the result of several years’ deposition. These factors caused As to have a high and toxic range in the Baneh trees. In addition, most metal ions exhibited the lowest mobility in soils with a high pH (> 7). However, the mobility rate of ions in soils can be enhanced with a change in pH (> 5) conditions (Kabata-Pendias [15]; Serbula et al. [39]).
In line with our results, the pH of the soils was more than 7, indicating that the TEs had poor mobility in the soils, which Baneh roots slowly absorbed. Serbula et al. ([39]) found that Pb, Cu, and Zn have BCF < 1 in the leaves of Robinia pseudoacacia L. from various locations. Found they were poorly absorbed by the soil, resulting in soils with a pH greater than 7. The elements of Pb and As in barks of Baneh trees, on the other hand, were highly absorbed from soils and had a higher BCF. Bing et al. ([7]) showed that in air pollution conditions, As, Cr, Mn, Ni, and S with low BCF had a lower ability to be absorbed from the soil portions than other elements. In our case, elements were efficiently accumulated in the foliar organs of Baneh trees because of the slow absorption capacity.
Trace elements are available in two forms: soluble and crystallized. Besides, their accumulation in trees is related to different tree organs and types of elements (Alatou and Sahli [3]). The greatest metal accumulation sub-indexes were for sulfur in leaves and copper in barks. As compared to barks, leaves had higher MAI, which showed that the leaves were more enriched with studied elements from the air than barks. Accordingly, these trees are suitable for accumulating atmospheric elements emitted from the IGR. Tree barks with their structure to retain atmospheric pollutants and metals can give a valuable monitoring basis for long-term air pollution surveys (Berlizov et al. [6]). With their unique structures and characteristics, tree barks and leaves have varying capabilities for accumulating metallic contamination from the soil or air. Deposition in the outer surface of barks and translocation through the xylem from roots are the essential factors for TEs accumulation in tree barks (Alatou and Sahli [3]). According to Krämer ([18]), metal transportation system efficiency, antioxidant defense responses, and overall physiological status are significant indicator factors of element amounts transferred to the aboveground parts of trees.
As is the persistent carcinogen and global metalloid that had high bioaccumulation in the environment. It has harmful effects on the metabolism and bioenergetics of tissues in plants, animals, and humans. Different ills related to poisons of this metalloid are in the air, soil, and water (Kaur et al. [16]). The trees' anatomical characteristics, such as the structure of different tissues, pore cells, and leaf area, are the most important determining factors of absorption and accumulation of air pollutants and toxic elements by trees (Safari et al. [38]). Almost all the studied elements in this work accumulated in the leaf organs. Among TEs, the most dangerous was detecting a high concentration of As in leaves and barks of analyzed trees. Arsenic was found at significant concentrations in both the atmosphere and the soil. Furthermore, it was found in dangerous concentrations in soil samples from the Zagros area, which might cause severe physiological and structural disorders.
Conclusions
Sulfur and TEs concentrations in contamination seasons (summer and autumn) and distances (up to 2500 m) in soils and Baneh tree components differed from the spring and control distance. The concentrations of S, As, Ni, and Cr were higher than Iranian and international soil standards. Soil features, including low EC, alkaline conditions, and abundant clay particles, were the major causes of soil element contamination. Because of the poor mobility and availability of the soil's trace elements (e.g., weaker extractions), trees slightly absorbed and accumulated elements from soil sections. As opposed to the barks, the leaves have the largest elemental accumulation capacity due to the different anatomical structures and sensitivities of the organs to atmospheric elements. The PCA findings revealed that the IGR's air pollution directly impacted the concentrations of all components in soils, barks, and leaves. Food systems may be disrupted by potentially harmful materials in soils and trees, putting the health and lives of wildlife in jeopardy. As a result, it is advised that the risk level to the food web in the Zagros habitats surrounding the gas refinery be assessed regularly.
Data availability
The data used to support the findings of this study are available from the corresponding author upon reasonable request.
Code availability
Not applicable.
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The authors are grateful for the ecology lab of Tarbiat Modares University for soil properties analysis.
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HD conceived the idea and acquired funding, carried out field and experimental studies, analyzed and curated the data, structured, wrote, and improved the manuscript. NP contributed to the final version of the manuscript with software, data interpretation, and review-editing.
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Dadkhah-Aghdash, H., Pehlivan, N. The organ level atmospheric element signatures of native Pistacia atlantica in semi-arid forests linked to the Ilam Gas Refinery, Iran. Plant Soil 475, 293–308 (2022). https://doi.org/10.1007/s11104-022-05368-7
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DOI: https://doi.org/10.1007/s11104-022-05368-7