Abstract
Microglia clean up dead cells and debris through phagocytosis in the central nervous system. UDP-activated P2Y6 receptors (P2Y6Rs) induce the formation of phagocytic cup-like structure and P2Y6R expression is increased during the phagocytosis. However, it remains unclear how surface expression of P2Y6R is increased. PICK1 (protein interacting with C-kinase-1) interacts with various neurotransmitter receptors, transporters, and enzymes. We here report that PICK1 might interact with P2Y6R. Surface P2Y6R was reduced in microglia from PICK1-knockout mice and PICK1-knockdown BV2 cells, which was also confirmed by electrophysiological recordings, showing that P2Y6R-mediated current was increased by PICK1 overexpression but was reduced by PICK1-knockdown in BV2 microglia. Finally, PICK1 was sufficient to affect cytoskeletal aggregation and phagocytosis both in primary microglia and BV2 cells. These results indicate that PICK1 is an important regulator of P2Y6R expression and microglial phagocytosis.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Microglia, the immune cells in the central nervous system (CNS) [1], detect pathological changes in the microenvironment and quickly respond to maintain CNS homeostasis [2]. During CNS development, microglia can swallow excess synapses, thereby regulating the number of synapses [3]. Under pathological conditions, microglia clean up dead cells and debris through the process of phagocytosis, thereby reducing the secondary damage to CNS [4, 5]. Hence, studies on phagocytosis are important to understand roles of microglia in CNS.
It has been shown that receptors on the surface of microglia are remarkably sensitive to subtle changes in microenvironment [6, 7]. Notably, P2Y6 receptor (P2Y6R), a G-protein-coupled purinergic receptor, is critical to microglial phagocytosis [8, 9]. P2Y6R is activated by UDP released by damaged neurons, and surrounds larger external particles through membrane ruffle. Meanwhile, mRNA and protein levels of P2Y6R in microglia are also increased, and the phagocytosis can be blocked by P2Y6R antagonists [8, 9]. In fact, P2Y6R deficiency is closely linked to neurodegenerative and immune diseases in CNS [10]. These findings highlight critical functions of P2Y6R in regulating microglial phagocytosis. However, it remains unclear how P2Y6R expression is increased and how P2Y6R is transported to plasma membrane.
PICK1 (protein interacting with C-kinase-1) is first cloned as a protein kinase C (PKC)-binding protein [11]. It is the only known protein that possesses a PSD-95/DlgA/ZO-1 (PDZ) domain and a Bin-Amphiphysin-Rvs (BAR) domain, and has received considerable attention [12]. Through the PDZ domain, PICK1 interacts with various neurotransmitter receptors, transporters, and enzymes [12, 13]. Through the BAR domain, PICK1 anchors its partners and influences their synaptic localization and function [12, 13]. The best-characterized function of PICK1 is that it regulates trafficking of GluRA2 subunit of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor (AMPAR) during long-term depression and long-term potentiation [12–16].
In the present work, we found that PICK1 interacted with P2Y6R in vivo and in cultured microglia and BV2 cells. Overexpression of PICK1 in BV2 increased surface expression of P2Y6R and UDP-induced currents. On the contrary, PICK1-knockdown decreased P2Y6R currents and surface expression in BV2 cells, which was confirmed by the reduced surface expression of P2Y6R in cultured microglia derived from PICK1−/− mice. Finally, PICK1 expression was sufficient to change the cytoskeletal arrangement and phagocytosis in microglia. In summary, our work first suggests that PICK1 is an important regulator of P2Y6R expression and microglial phagocytosis.
Experimental and Methods
Animals
All experiments were approved by the Animal Experimentation Ethics Committees of Jiaxing University and Zhejiang University, and were specifically designed to minimize the number of animals used. PICK1-knockout mice were the same as used in previous work [17] and maintained at the Experimental Animal Center of Zhejiang University. Mice were kept under temperature-controlled conditions on a 12:12 h light/dark cycle with food and water ad libitum.
Antibodies and Reagents
Antibodies against P2Y6R (#ab92504), P2Y4R (#ab180718), Flag (#ab1162), GFP (#ab6556), and GAPDH (#ab181602) were from Abcam (Cambridge, UK). Antibody to flotillin-1 (#61020) was from BD Biosciences (San Jose, CA). Antibody to ionized Ca2+-binding adapter molecule 1 (Iba1) (#01919741) was from Wako (Osaka, Japan). Mouse anti-PICK1 antibody (#L20-8) was from NeuroMab (Davis, CA) and rabbit polycolonal anti-PICK1 antibody was from Jun Xia (Hong Kong). Horseradish peroxidase-conjugated secondary antibodies (#31420 and #31460) were from Thermo (Rockford, IL). IgG antibody (#PA5-23094), Alexa Fluor 543-conjugated secondary antibody (#A-11037), Alexa Fluor 488-conjugated secondary antibody (#A-10034), Alexa Fluor 350-conjugated secondary antibody (#A-10039), Alexa Fluor 594-conjugated phalloidin, Alexa Fluor 594 Zymosan A-BioParticles conjugate, and X-rhod-1-AM were from Invitrogen (Carlsbad, CA). The protease inhibitor cocktail was from Merck Chemicals (Darmstadt, Germany). Latruncullin A was from Cayman (Ann Arbor, Michigan). Other chemicals were from Sigma (St. Louis, MO) unless stated otherwise.
BV2 Microglia Culture
The murine BV2 cell line was maintained in DMEM supplemented with 10 % FBS, 100 U ml−1 penicillin and 100 µg ml−1 streptomycin at 37 °C in a humidified incubator with 5 % CO2. Confluent cultures were passaged by trypsinization. Cells were washed twice with warm DMEM and treated in serum-free medium for at least 4 h before experiments.
Primary Microglia-Enriched Culture
Microglial cells were prepared from the cortex of P1 mice as described previously [18]. Briefly, cortex was gently triturated and seeded (5 × 107) in 150 cm2 flasks. Two weeks after seeding, microglial cells were separated from astrocytes by shaking the flasks for 5 h at 150 g and were replaced at 1 × 105 in a 24-well plate precoated with poly-d-lysine. The enriched microglia were >95 % pure, as determined by immunostaining with antibody against F4/80 (eBioscience, San Diego, CA).
Lentivirus Transfection
The lentivirus transfection was performed according to previous work [17, 19]. Lentivirus encoding scrambled RNA, PICK1 shRNA, or PICK1 was prepared by GenePharma (Shanghai, China) using entire gene sequence of rat PICK1 (GenBank ID: AF542094.1), and each RNA was tagged with GFP. The sequence for PICK1 shRNA was 5′-GCC TCA CCA TCA AGA AGT ACC-3′ [19]. PICK1 shRNA and scrambled shRNA were driven by U6 promoter, whereas overexpressed PICK1 was driven by elongation factor 1α promoter. pVSV-G plasmid driven by the CMV promoter expressed envelope glycoprotein of vesicular stomatitis virus and thus replaced the lentiviral env gene. All virus-packed plasmids were transduced into cultures at 20 multiplicities 2 days before experiments. Only when >90 % of cultured cells were transfected, which was confirmed by GFP fluorescence, experiments were continued.
Surface Biotinylation
Cultured cells were rinsed with cold phosphate-buffered saline (PBS) containing 1 mM MgCl2 and 0.5 mM CaCl2, and incubated in 1 mg/ml sulfo-NHS-LC-biotin (Thermo) for 30 min at 4 °C. Cells were quenched with 100 mM glycine for 15 min at 4 °C, lysed in ice-cold RIPA buffer (50 mM Tris–HCl, 0.1 % SDS, 0.5 % sodium deoxycholate, 1 % Triton-X-100), and centrifuged for 20 min at 4 °C. The BCA assay (Thermo) was used to measure the concentration of proteins in the supernatant, which was next incubated with NeutrAvidin beads (Thermo) for 2 h at 4 °C. Biotinylated proteins were extracted using 2 × SDS sample buffer supplemented with dithiothreitol (50 mM) for 30 min at 50 °C prior to Western blotting.
Co-Immunoprecipitation (Co-IP)
Co-IP experiments were performed according to previous work [20]. In brief, protein concentration was measured using the BCA assay after centrifugation at 16,000g at 4 °C for 10 min. Supernatant (15 μg) was used for input and the remainder was for IP. Pre-cleared solubilized preparations were incubated with anti-PICK1 antibody, which was pre-coupled to protein A-Sepharose beads (GE Healthcare, Waukesha, WI) at 2–4 μg antibody/1 ml beads for 2 h in 50 mM Tris–HCl. Proteins on the beads were extracted with 2 × SDS sample buffer and boiled for 5 min before Western blotting.
Western Blotting
Western blotting was performed as reported previously [20]. In brief, equal amounts of proteins were loaded and fractionated on 10 % sodium dodecyl sulfate–polyacrylamide gels and transferred to PVDF membrane (Millipore, Billerica, MA), immunoblotted with antibodies, and visualized by enhanced chemiluminescence (Pierce Biotechnology, Rockford, IL). The primary antibody dilutions used were P2Y6R (1:500), P2Y4R (1:500), PICK1 (1:2000), flotillin-1 (1:10,000), GAPDH (1:10,000), and secondary antibodies (1:10,000). Film signals were digitally scanned and quantitated using ImageJ 1.42q (NIH, Bethesda, MD).
Immunocytochemistry
Immunofluorescent staining was performed as described previously [21]. For intracellular staining, cells were fixed 48 h after transfection by 4 % paraformaldehyde and 4 % sucrose in PBS for 15 min, and then permeabilized by 0.2 % Triton X-100 in PBS for 15 min. After blocking with 10 % Bovine Serum Albumin (BSA) for 1 h, transfected BV2 and microglia were incubated with anti-Iba1 antibody (1:500) overnight and another 1-hr incubation with Alexa Fluor 350-conjugated secondary antibody (1:800). For surface staining, transfected BV2 cells were fixed in 4 % paraformaldehyde for 5 min and blocked with BSA for 10 min. These cells were next incubated in anti-Flag antibody (1:500) for 15 min, rinsed for three times with PBS, and incubated with Alexa Fluor 543-conjugated secondary antibody (1:800). To examine F-actin aggregation, Alexa Fluor 594-conjugated phalloidin was added to microglia cultures and BV2 cells. Cells were imaged on a confocal microscope (Olympus, Tokyo, Japan). All parameters used in confocal microscopy were consistent in each experiment, including laser excitation power, detector, and off-set gains. The thickness of the optical slice (pinhole) was adjusted to 0.7 μm and z-stacks were acquired at 50 % overlap to detect surface expression of N-terminal Flag-tagged P2Y6R, according to previous work [22]. Image J software (NIH, Bethesda, MD) was used to analyze cell images. Imaging data analysts were blind to the experimental conditions until the data were integrated.
Phagocytosis Assay
The experiment of phagocytosis assay was performed according to previous work [23]. BV2 or microglia cells were incubated with fluorescent Zymosan A (5 μg/ml) for 30 min at 37 °C in a humidified incubator with 5 % CO2, and then washed with normal medium for several times to remove excess beads. The number of beads per cell was measured for statistics using a confocal microscope (Olympus). At least 20 cells were added to data pool for each experimental condition.
Electrophysiology
Electrophysiological recordings was performed according to previous work [20, 24]. Cells were placed in a perfusion chamber with continuous gravity flow (2 ml/min) of Ringer’s containing (in mM) 125 NaCl, 2.5 KCl, 1.25 NaH2PO4, 1 MgCl2, 2 CaCl2, 26 NaHCO3 and 25 d-glucose (pH7.4). Recording electrodes (3–5 MΩ) for whole-cell patch clamp contained (in mM) 135 CsMS, 10 CsCl, 10 HEPES, 0.2 EGTA, 4 Na2ATP, and 0.4 Na3GTP (pH 7.3, OSM 300). The final concentration of UDP was 100 μM in the work solution. Cells were voltage-clamped at −70 mV using a 700A amplifier (Molecular Devices, Foster City, CA). The analog signals were sampled at 10 kHz and low-pass filtered at 2 kHz using pClamp 9.0 (Molecular Devices). To measure P2Y6R currents, a rapid solution changer (RSC-160, Biological Science Instruments, Claix, France) was used to apply drug solutions to recorded cells. The protocol of drug application was controlled by the analog output from the pClamp program.
Ca2+ Imaging
[Ca2+]i was determined using Ca2+-sensitive dye X-rhod-1-AM according to previous work [25]. BV2 cells were incubated with membrane-permeable X-rhod-1-AM (5 μM) for 30 min at RT in the dark, and were then transferred to a recording chamber. Ca2+ transients were elicited by local application of UDP (100 μM) and recorded using a confocal microscope (Olympus) with the excitation wavelength of 580 nm and the emission wavelength of 645 nm. X-rhod-1 images were sampled in frame-scan mode at a frame rate of 0.1 Hz. For analysis, a region of interest (ROI) that applied to all frames in a series of images was determined by threshold foreground pixels, and these foreground pixels were spatially averaged to calculate F for each frame. First three frames in each experiment were averaged and used as F 0. Levels of [Ca2+]i were expressed as F/F 0 ratios.
Statistical Analysis
Data analysis was performed using Excel 2003 (Microsoft, Seattle, WA), Igor Pro 6.0 (Wavemetrics, Lake Oswego, OR), and SPSS 16.0 statistical program (Chicago, IL). Statistical difference was determined using unpaired two-sided Student’s t test. The accepted level of significance was p < 0.05. “n” represents the number of cells or cultures tested. Data in the text and figures are presented as mean ± SEM.
Results
The expression of endogenous PICK1 and P2Y6R were examined in BV2 microglia. As shown by the immunochemical staining obtained from confocal microscopy (Fig. 1a), both PICK1 and P2Y6R were robustly expressed in BV2 cells and displayed a diffuse pattern in the cytosol but not nucleus. Moreover, the distribution pattern of PICK1 was very similar to that of P2Y6R (Fig. 1a), implying that that PICK1 might interact with P2Y6R. To investigate the potential interaction between them, Co-IP assay was performed. First, lysates from mouse cortex were immunoprecipitated with PICK1 antibody. We found that endogenous P2Y6R, but not P2Y4R, was pulled down by PICK1 when PICK1 was immunoprecipitated (Fig. 1b). Second, Co-IP assay was performed in the primary microglial culture. Likewise, anti-PICK1 antibody pulled down P2Y6R but not P2Y4R (Fig. 1c). PICK1 did not pull down IgG, showing the specificity of Co-IP experiments (Fig. 1c). Together, these results suggest that PICK1 may associate with P2Y6R.
We next investigated whether PICK1 affects the expression of P2Y6R. Microglial cells from wild-type and PICK1-knockout mice were cultured. Surface biotinylation and Western blotting were used to detect total and surface expressions of P2Y6R in primary microglia. Our results showed that total expression of P2Y6R was not changed in PICK1-knockout microglia (Fig. 2a). However, the amount of P2Y6R on the cell surface was decreased significantly in PICK1-knockout microglia (Fig. 2a), suggesting that PICK1-knockout attenuates P2Y6R on the surface of microglia. To further confirm this result, BV2 cells were transfected with plasmid encoding N-terminal Flag-tagged P2Y6R, meanwhile they were also transduced with lentivirial GFP-tagged scrambled RNA, GFP-PICK1-shRNA, or GFP-PICK1. As shown in Fig. S1, PICK1-shRNA knockdown effectively suppressed the expression of PICK1 in BV2 cells, consistent with previous work (17, 19). Surface expression of P2Y6R was examined 2 days after the transfection using the antibody against Flag. Our results showed that the intensity of Flag was much reduced in PICK1-shRNA group, but was significantly increased in PICK1 group (Fig. 2b). Meanwhile, the fluorescent intensity of tagged-GFP with viral transfection appeared same among three groups (Fig. 2b). These results confirm that surface expression of P2Y6R is modulated by PICK1.
We next investigated whether PICK1 influences the function of P2Y6R. BV2 cells were transduced with control lentivirus, PICK1-shRNA, or PICK1, and whole-cell P2Y6R currents was evoked by UDP, which was the specific agonist for P2Y6R [26]. As shown in Fig. 3a, the peak of P2Y6 currents was significantly increased by PICK1 overexpression. On contrary, P2Y6R current was significantly suppressed by PICK1-knockdown compared to either NC or PICK1 group (Fig. 3a). Since activation of P2Y6R leads to an increase in intracellular Ca2+, we next investigated whether PICK1 influences P2Y6R-mediated [Ca2+]i. Three different groups of BV2 cells were incubated with a membrane-permeable Ca2+ indicator, X-rhod-1-AM (5 μM). Confocal imaging showed that UDP induced an increase in Ca2+ fluorescence in NC group (Fig. 3b). When BV2 cells were transfected with PICK1-shRNA, UDP application did not induce an obvious increase of [Ca2+]i (Fig. 3c). When BV2 cells were transfected with PICK1, UDP application induced a remarkable increase of [Ca2+]i (Fig. 3c). Taken together, these results indicate that PICK1 is essential to P2Y6R-mediated currents and Ca2+ transient.
It has been shown that the activation of P2Y6R is sufficient to affect cytoskeletal arrangement and formation of “phagocytic cup” in microglial cells [27]. Given the significance of PICK1 on the activation of P2Y6R, we examined if PICK1 is able to affect cytoskeletal arrangement in BV2 cells and microglia. BV2 cells were transduced with control lentivirus or lentiviral plasmid encoding PICK1 and then subjected to UDP stimulation. The pattern of F-actin was indicated by phalloidin staining and the efficiency of transfection was shown by GFP. In control, UDP stimulation (3 min) caused relatively little actin polymerization, shown as concentrated phalloidin staining in the peripheral part of plasma membrane (Fig. 4a). In the condition of PICK1 overexpression, the number of actin aggregation induced by UDP stimulation was markedly increased (Fig. 4b). Similar experiments were conducted in cultured microglia. Likewise, UDP stimulation (3 min) caused less actin polymerization in control microglia (Fig. 4c), whereas the number of actin aggregation was significantly increased in the condition of PICK1 overexpression (Fig. 4d). Taken together, these data indicate that PICK1 is sufficient to facilitate UDP-induced actin aggregation and ruffle formation.
Finally, we investigated whether PICK1 is sufficient to affect phagocytic capacity of BV2 cells and primary microglia. First, BV2 cells were transduced with control lentivirus, PICK1-shRNA, or PICK1, and then presented with fluorescent Zymosan-A microbeads and UDP (100 μM) for 30 min [23]. There was no difference in the number of microbeads among three groups before UDP treatment (Fig. S2). However, PICK1-shRNA significantly reduced phagocytic capacity of BV2 cells (shRNA: 1.2 ± 0.5 beads/cell; n = 20 cells) compared to control (NC: 3.0 ± 0.5 beads/cell; n = 21 cells), whereas PICK1 remarkably increased phagocytic capacity of BV2 cells (PICK1: 5.0 ± 1.1 beads/cell; n = 21 cells) (Fig. 5a). Second, primary microglia culture from WT and PICK1-knockout mice were presented with fluorescent Zymosan-A microbeads and UDP (100 μM) for 30 min to assay phagocytic capacity [23]. In consistent with BV2 cells, the number of beads in PICK1-knockout microglial cells was significantly decreased compared to WT (WT: 7.7 ± 2.6 beads/cell, n = 20 cells; knockout: 2.1 ± 1.1 beads/cell, n = 20 cells) (Fig. 5b). Thus, these results indicate that PICK1 facilitates microglial phagocytic activity.
Discussion
In the present work, we showed that PICK1 was associated with P2Y6R in BV2 cells and cultured microglia. PICK1 might engage in the trafficking of P2Y6R, which was suggested that its surface expression (but not total), its-mediated current, and its-mediated Ca2+ transient were all regulated by PICK1. Importantly, PICK1 was sufficient to enhance actin polymerization to shape filopodia-like protrusions and phagocytosis. In summary, these results indicated that PICK1 may have essential roles in microglial phagocytosis through modulating P2Y6R.
Microglia are quite sensitive to brain damage. They are immediately activated in response to brain injury and migrate to the sites of injured cells or their debris [2]. These phenotypes show that microglial phagocytosis is critical to the reconstruction after brain damage and is thereby beneficial to the brain [2]. However, functions of microglia are yet under controversial because over-activated microglia also cause cell loss [2]. It has been shown that P2Y6R plays important roles in microglial phagocytosis. For example, UDP released from damaged cells acts as an “eat-us” signal and triggers the phagocytosis through P2Y6R [6]. Moreover, P2Y6R is activated and its level on the membrane is elevated during the phagocytosis [13, 14]. Despite these findings, it remains unsolved how P2Y6R and its activity are regulated during the phagocytosis. In the present work, we added an important signaling to the underlying mechanism for microglial phagocytosis by demonstrating that PICK1, a peripheral protein, affects the targeting of P2Y6R to cell surface. Critical evidence for our conclusion includes: (1) Surface expression of P2Y6R was significantly increased in BV2 cells when PICK1 was co-expressed; (2) Number of actin aggregation induced by UDP was markedly increased with PICK1 over-expression in BV2 cells and primary micorglia; (3) Phagocytic capacity of BV2 cells and primary microglia was increased when PICK1 was over-expressed but was decreased by PICK1-knockdown or PICK1-knockout.
Although our evidence suggests that PICK1 may affect the trafficking of P2Y6R in microglia, how PICK1 increases surface expression of P2Y6R is not solved in the present work. Through PDZ domain and BAR domain, PICK1 interacts with receptors, transporters, and enzymes, and thereby participates in many aspects of brain function [12, 13, 28]. We recently show that PICK1 modulates membrane expression of excitatory amino acid transporter 3 (EAAT3) through Rab11-positive recycling endosomes [17]. In addition, PICK1 is shown to help the transport of insulin-containing mature secretory granules to the membrane and facilitate insulin secretion [29]. Thus, it is conceivable that PICK1 is involved in the formation of different trafficking vesicles, which provides a potential mechanism to enriched membrane P2Y6R.
Conclusion
PICK1 interacts with P2Y6R in microglia. It thereby regulates the surface expression of P2Y6R and its-mediated currents. Moreover, PICK1 modulates the aggregation of F-actin and phagocytosis in BV2 cells and primary microglia.
Abbreviations
- AMPAR:
-
Α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor
- BAR:
-
Bin-Amphiphysin-Rvs
- Iba1:
-
Ca2+-binding adapter molecule 1
- PICK1:
-
Protein interacting with C-kinase 1
- PDZ:
-
PSD-95/DlgA/ZO-1
- PKC:
-
Protein kinase C
- PBS:
-
Phosphate-buffered saline
References
Aloisi F (2001) Immune function of microglia. Glia 36:165–179
Hanisch UK, Kettenmann H (2007) Microglia: active sensor and versatile effector cells in the normal and pathologic brain. Nat Neurosci 10:1387–1394
Ji K, Akgul G, Wollmuth LP, Tsirka SE (2013) Microglia actively regulate the number of functional synapses. PLoS One 8:e56293
Sierra A, Abiega O, Shahraz A, Neumann H (2013) Janus-faced microglia: beneficial and detrimental consequences of microglial phagocytosis. Front Cell Neurosci 7:6
Chan A, Hummel V, Weilbach FX, Kieseier BC, Gold R (2006) Phagocytosis of apoptotic inflammatory cells downregulates microglial chemoattractive function and migration of encephalitogenic T cells. J Neurosci Res 84:1217–1224
Koizumi S, Ohsawa K, Inoue K, Kohsaka S (2013) Purinergic receptors in microglia: functional modal shifts of microglia mediated by P2 and P1 receptors. Glia 61:47–54
Mika T, Prochnow N (2012) Functions of connexins and large pore channels on microglial cells: the gates to environment. Brain Res 1487:16–24
Koizumi S, Shigemoto-Mogami Y, Nasu-Tada K, Shinozaki Y, Ohsawa K, Tsuda M, Joshi BV, Jacobson KA, Kohsaka S, Inoue K (2007) UDP acting at P2Y6 receptors is a mediator of microglial phagocytosis. Nature 446:1091–1095
Inoue K (2007) UDP facilitates microglial phagocytosis through P2Y6 receptors. Cell Adh Migr 1:131–132
Zhang Z, Wang Z, Ren H, Yue M, Huang K, Gu H, Liu M, Du B, Qian M (2011) P2Y(6) agonist uridine 5′-diphosphate promotes host defense against bacterial infection via monocyte chemoattractant protein-1-mediated monocytes/macrophages recruitment. J Immunol 186:5376–5387
Staudinger J, Zhou J, Burgess R, Elledge SJ, Olson EN (1995) PICK1: a perinuclear binding protein and substrate for protein kinase C isolated by the yeast two-hybrid system. J Cell Biol 128:263–271
Xu J, Xia J (2006) Structure and function of PICK1. Neurosignals 15:190–201
Hanley JG (2008) PICK1: a multi-talented modulator of AMPA receptor trafficking. Pharmacol Ther 118:152–160
Hanley JG, Henley JM (2005) PICK1 is a calcium-sensor for NMDA-induced AMPA receptor trafficking. EMBO J 24:3266–3278
Steinberg JP, Takamiya K, Shen Y, Xia J, Rubio ME, Yu S, Jin W, Thomas GM, Linden DJ, Huganir RL (2006) Targeted in vivo mutations of the AMPA receptor subunit GluR2 and its interacting protein PICK1 eliminate cerebellar long-term depression. Neuron 49:845–860
Terashima A, Pelkey KA, Rah JC, Suh YH, Roche KW, Collingridge GL, McBain CJ, Isaac JT (2008) An essential role for PICK1 in NMDA receptor-dependent bidirectional synaptic plasticity. Neuron 57:872–882
Wang YN, Zhou L, Li YH, Wang Z, Li YC, Zhang YW, Wang Y, Liu G, Shen Y (2015) Protein interacting with C-Kinase 1 deficiency impairs glutathione synthesis and increases oxidative stress via reduction of surface excitatory amino acid carrier 1. J Neurosci 35:6429–6443
Liu B, Wang K, Gao HM, Mandavilli B, Wang JY, Hong JS (2001) Molecular consequences of activated microglia in the brain: overactivation induces apoptosis. J Neurochem 77:182–189
Anggono V, Clem RL, Huganir RL (2011) PICK1 loss of function occludes homeostatic synaptic scaling. J Neurosci 31:2188–2196
Zhu J, Shao CY, Yang W, Zhang XM, Wu ZY, Zhou L, Wang XX, Li YH, Xia J, Luo JH, Shen Y (2012) Chronic zinc exposure decreases the surface expression of NR2A-containing NMDA receptors in cultured hippocampal neurons. PLoS One 7:e46012
Xie YJ, Zhou L, Jiang N, Zhang N, Zou N, Zhou L, Wang Y, Cowell JK, Shen Y (2015) Essential roles of leucine-rich glioma inactivated 1 in the development of embryonic and postnatal cerebellum. Sci Rep 5:7827
Nguyen TT, Kim YM, Kim TD, Le OT, Kim JJ, Kang HC, Hasegawa H, Kanaho Y, Jou I, Lee SY (2013) Phosphatidylinositol 4-phosphate 5-kinase α facilitates Toll-like receptor 4-mediated microglial inflammation through regulation of the Toll/interleukin-1 receptor domain-containing adaptor protein (TIRAP) location. J Biol Chem 288:5645–5659
Neniskyte U, Neher JJ, Brown GC (2011) Neuronal death induced by nanomolar amyloid β is mediated by primary phagocytosis of neurons by microglia. J Biol Chem 286:39904–39913
Wang DJ, Su LD, Wang YN, Yang D, Sun CL, Zhou L, Wang XX, Shen Y (2014) Long-term potentiation at cerebellar parallel fiber-Purkinje cell synapses requires pre- and postsynaptic signaling cascades. J Neurosci 34:2355–2364
Wu ZY, Zhu LJ, Zou N, Bombek LK, Shao CY, Wang N, Wang XX, Liang L, Xia J, Rupnik M, Shen Y (2012) AMPA receptors regulate exocytosis and insulin release in pancreatic β cells. Traffic 13:1124–1139
Mosbacher J, Maier R, Fakler B, Glatz A, Crespo J, Bilbe G (1998) P2Y receptor subtypes differentially couple to inwardly-rectifying potassium channels. FEBS Lett 436:104–110
Liu GD, Ding JQ, Xiao Q, Chen SD (2009) P2Y6 receptor and immunoinflammation. Neurosci Bull 25:161–164
Deken SL, Beckman ML, Quick MW (2001) PICKing on transporters. Trends Neurosci 24:623–625
Cao M, Mao Z, Kam C, Xiao N, Cao X, Shen C, Cheng KKY, Xu A, Lee KM, Jiang L, Xia J (2013) PICK1 and ICA69 control insulin granule trafficking and their deficiencies lead to impaired glucose tolerance. PLoS Biol 11:e1001541
Acknowledgments
This work was supported by grants from National Natural Science Foundation of China (81501043, 31460257, 31471024, and 81571098).
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
All authors declare that there are no conflicts of interest on the paper.
Additional information
Jia Zhu and Zhen Wang have contributed equally to this work.
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Zhu, J., Wang, Z., Zhang, N. et al. Protein Interacting C-Kinase 1 Modulates Surface Expression of P2Y6 Purinoreceptor, Actin Polymerization and Phagocytosis in Microglia. Neurochem Res 41, 795–803 (2016). https://doi.org/10.1007/s11064-015-1754-3
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11064-015-1754-3