Abstract
HTm4 is a member of a newly defined family of human and murine proteins, the MS4 (membrane-spanning four) protein group, which has a distinctive four-transmembrane structure. MS4 protein functions include roles as cell surface signaling receptors and intracellular adapter proteins. We have previously demonstrated that HTm4 regulates the function of the KAP phosphatase, a key regulator of cell cycle progression. In humans, the expression of HTm4 is largely restricted to cells of the hematopoietic lineage, possibly reflecting a causal role for this molecule in differentiation/proliferation of hematopoietic lineage cells. In this study, we show that, like the human homologue, murine HTm4 is also predominantly a hematopoietic protein with distinctive expression patterns in developing murine embryos and in adult animals. In addition, we observed that murine HTm4 is highly expressed in the developing and adult murine nervous system, suggesting a previously unrecognized role in central and peripheral nervous system development.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
CN Adra JM Lelias H Kobayashi M Kaghad P Morrison JD Rowley B Lim (1994) ArticleTitleCloning of the cDNA for a hematopoietic cell-specific protein related to CD20 and the beta subunit of the high-affinity IgE receptor: evidence for a family of proteins with four membrane-spanning regions Proc Natl Acad Sci USA 91 10178–10152 Occurrence Handle1:CAS:528:DyaK2MXht1altbY%3D Occurrence Handle7524084
CN Adra D Manor JL Ko S Zhu T Horiuchi Aelst L Van RA Cerione B Lim (1997) ArticleTitleRhoGDIgamma: a GDP-dissociation inhibitor for Rho proteins with preferential expression in brain and pancreas Proc Natl Acad Sci USA 94 4279–4284 Occurrence Handle1:CAS:528:DyaK2sXjtVyhsr8%3D Occurrence Handle9113980
CN Adra XQ Mao H Kawada PS Gao B Korzycka JL Donate SR Shaldon P Coull M Dubowitz T Enomoto A Ozawa SA Syed T Horiuchi R Khaeraja R Khan SR Lin F Flinter P Beales A Hagihara H Inoko T Shirakawa JM Hopkin (1999) ArticleTitleChromosome 11q13 and atopic asthma Clin Genet 55 431–437 Occurrence Handle1:STN:280:DyaK1MznvVSlug%3D%3D Occurrence Handle10450859
S Akira H Isshiki T Sugita O Tanabe S Kinoshita Y Nishio T Nakajima T Hirano T Kishimoto (1990) ArticleTitleA nuclear factor for IL-6 expression (NF-IL6) is a member of a C/EBP family Embo J 9 1897–1906 Occurrence Handle1:CAS:528:DyaK3cXltFemsrk%3D Occurrence Handle2112087
JC Barnhill AJ Stokes M Koblan-Huberson LM Shimoda A Muraguchi CN Adra H Turner (2004) ArticleTitleRGA protein associates with a TRPV ion channel during biosynthesis and trafficking J Cell Biochem 91 808–820 Occurrence Handle1:CAS:528:DC%2BD2cXitlGisLc%3D Occurrence Handle14991772
JM Collee LP ten Kate HG de Vries JW Kliphuis K Bouman H Scheffer J Gerritsen (1993) ArticleTitleAllele sharing on chromosome 11q13 in sibs with asthma and atopy Lancet 342 936 Occurrence Handle1:STN:280:DyaK2c%2FhvVWgug%3D%3D Occurrence Handle8105198
JL Donato J Ko JL Kutok T Cheng T Shirakawa XQ Mao D Beach DT Scadden MH Sayegh CN Adra (2002) ArticleTitleHuman HTm4 is a hematopoietic cell cycle regulator J Clin Invest 109 51–58 Occurrence Handle1:CAS:528:DC%2BD38XjtlaktA%3D%3D Occurrence Handle11781350
R Folster-Holst HW Moises L Yang W Fritsch J Weissenbach E Christophers (1998) ArticleTitleLinkage between atopy and the IgE high-affinity receptor gene at 11q13 in atopic dermatitis families Hum Genet 102 236–239 Occurrence Handle1:CAS:528:DyaK1cXhtV2rs7s%3D Occurrence Handle9521597
K Georgopoulos M Bigby JH Wang A Molnar P Wu S Winandy A Sharpe (1994) ArticleTitleThe Ikaros gene is required for the development of all lymphoid lineages Cell 79 143–156 Occurrence Handle1:CAS:528:DyaK2cXmslemtLc%3D Occurrence Handle7923373
GJ Hannon D Casso D Beach (1994) ArticleTitleKAP: a dual specificity phosphatase that interacts with cyclin- dependent kinases Proc Natl Acad Sci USA 91 1731–1735 Occurrence Handle1:CAS:528:DyaK2cXis1Cnu7k%3D Occurrence Handle8127873
MD Hulett E Pagler JR Hornby (2001) ArticleTitleCloning and characterization of a mouse homologue of the human haematopoietic cell-specific four-transmembrane gene HTm4 Immunol Cell Biol 79 345–349 Occurrence Handle1:CAS:528:DC%2BD3MXmsVOns78%3D Occurrence Handle11488981
K Ishibashi M Suzuki S Sasaki M Imai (2001) ArticleTitleIdentification of a new multigene four-transmembrane family (MS4A) related to CD20, HTm4 and beta subunit of the high-affinity IgE receptor Gene 264 87–93 Occurrence Handle1:CAS:528:DC%2BD3MXhs1Wit7g%3D Occurrence Handle11245982
J Kyte RF Doolittle (1982) ArticleTitleA simple method for displaying the hydropathic character of a protein J Mol Biol 157 105–132 Occurrence Handle1:CAS:528:DyaL38Xks1yjtro%3D Occurrence Handle7108955
Y Liang TR Buckley L Tu SD Langdon TF Tedder (2001) ArticleTitleStructural organization of the human MS4A gene cluster on Chromosome 11q12 Immunogenetics 53 357–368 Occurrence Handle1:CAS:528:DC%2BD3MXls1Kmtrw%3D Occurrence Handle11486273
Y Liang TF Tedder (2001) ArticleTitleIdentification of a CD20-, FcepsilonRIbeta-, and HTm4-related gene family: sixteen new MS4A family members expressed in human and mouse Genomics 72 119–127 Occurrence Handle1:CAS:528:DC%2BD3MXitlagsbY%3D Occurrence Handle11401424
S Mathas M Hinz I Anagnostopoulos D Krappmann A Lietz F Jundt K Bommert F Mechta-Grigoriou H Stein B Dorken C Scheidereit (2002) ArticleTitleAberrantly expressed c-Jun and JunB are a hallmark of Hodgkin lymphoma cells, stimulate proliferation and synergize with NF-kappa B Embo J 21 4104–4113 Occurrence Handle1:CAS:528:DC%2BD38XnvVGnur0%3D Occurrence Handle12145210
MA McDevitt RA Shivdasani Y Fujiwara H Yang SH Orkin (1997) ArticleTitleA ‘knockdown’ mutation created by cis-element gene targeting reveals the dependence of erythroid cell maturation on the level of transcription factor GATA-1 Proc Natl Acad Sci USA 94 6781–6785 Occurrence Handle1:CAS:528:DyaK2sXktF2rtbY%3D Occurrence Handle9192642
S Mitaku K Suzuki S Odashima K Ikuta M Suwa F Kukita M Ishikawa H Itoh (1995) ArticleTitleInteraction stabilizing tertiary structure of bacteriorhodopsin studied by denaturation experiments Proteins 22 350–362 Occurrence Handle1:CAS:528:DyaK2MXnslWrtL8%3D Occurrence Handle7479709
T Nakajima M Iikura Y Okayama K Matsumoto C Uchiyama T Shirakawa X Yang CN Adra K Hirai H Saito (2004) ArticleTitleIdentification of granulocyte subtype-selective receptors and ion channels by using a high-density oligonucleotide probe array J Allergy Clin Immunol 113 528–535 Occurrence Handle1:CAS:528:DC%2BD2cXhvFSltr8%3D Occurrence Handle15007357
PP Pandolfi ME Roth A Karis MW Leonard E Dzierzak FG Grosveld JD Engel MH Lindenbaum (1995) ArticleTitleTargeted disruption of the GATA3 gene causes severe abnormalities in the nervous system and in fetal liver haematopoiesis Nat Genet 11 40–44 Occurrence Handle1:CAS:528:DyaK2MXnvFelsLw%3D Occurrence Handle7550312
RY Poon T Hunter (1995) ArticleTitleDephosphorylation of Cdk2 Thr160 by the cyclin-dependent kinase- interacting phosphatase KAP in the absence of cyclin Science 270 90–93 Occurrence Handle1:CAS:528:DyaK2MXosFOmsLY%3D Occurrence Handle7569954
K Quandt K Frech H Karas E Wingender I Werner (1995) ArticleTitleMat Ind and Mat Inspector: new fast and versatile tools for detection of consensus matches in nucleotide sequence data Nuclear Acids Res 23 4878–4884 Occurrence Handle1:CAS:528:DyaK28XjsF2ksQ%3D%3D
C Ra MH Jouvin JP Kinet (1989) ArticleTitleComplete structure of the mouse mast cell receptor for IgE (Fc epsilon RI) and surface expression of chimeric receptors (rat-mouse-human) on transfected cells J Biol Chem 264 15323–15327 Occurrence Handle1:CAS:528:DyaK3cXitleltL4%3D Occurrence Handle2527850
JV Ravetch JP Kinet (1991) ArticleTitleFc receptors Annu Rev Immunol 9 457–492 Occurrence Handle1:CAS:528:DyaK3MXmt12ku7w%3D Occurrence Handle1910686
T Shirakawa T Hashimoto J Furuyama T Takeshita K Morimoto (1994) ArticleTitleLinkage between severe atopy and chromosome 11q13 in Japanese families Clin Genet 46 228–232 Occurrence Handle10.1111/j.1399-0004.1994.tb04231.x Occurrence Handle1:STN:280:DyaK2M7itV2jsQ%3D%3D Occurrence Handle7820936
TF Tedder M Streuli SF Schlossman H Saito (1988) ArticleTitleIsolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes Proc Natl Acad Sci USA 85 208–212 Occurrence Handle1:CAS:528:DyaL1cXktFOqt7c%3D Occurrence Handle2448768
FY Tsai G Keller FC Kuo M Weiss J Chen M Rosenblatt FW Alt SH Orkin (1994) ArticleTitleAn early haematopoietic defect in mice lacking the transcription factor GATA-2 Nature 371 221–226 Occurrence Handle1:CAS:528:DyaK2cXmt1Oit7Y%3D Occurrence Handle8078582
L van Herwerden SB Harrap ZY Wong MJ Abramson JJ Kutin AB Forbes J Raven A Lanigan EH Walters (1995) ArticleTitleLinkage of high-affinity IgE receptor gene with bronchial hyperreactivity, even in absence of atopy Lancet 346 1262–1265 Occurrence Handle1:STN:280:DyaK28%2FmsVyrsA%3D%3D Occurrence Handle7475718
Author information
Authors and Affiliations
Corresponding author
Additional information
JLK and XY contributed equally to this work
Rights and permissions
About this article
Cite this article
Kutok, J.L., Yang, X., Folkerth, R.D. et al. The cell cycle associated protein, HTm4, is expressed in differentiating cellsof the hematopoietic and central nervous system in mice. J Mol Hist 36, 77–87 (2005). https://doi.org/10.1007/s10735-004-3913-8
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/s10735-004-3913-8