Abstract
Genetic diversity and relationships among 48 safflower accessions were evaluated using 22 inter-simple sequence repeats (ISSR) primers. A total of 429 bands were amplified, and 355 bands (about 82.7%) were polymorphic. Five to forty-one polymorphic bands could be amplified by each primer, with an average of 16.1 polymorphic bands per primer. The results showed that the polymorphism of the safflower germplasm was higher at the DNA level. All the 48 accessions could be distinguished by ISSR markers and were divided into 9 groups based on ISSR GS by using UPGMA method. The genetic relationships among the accessions from different continents were closer. Comparatively, the genetic diversity of the accessions originated from Asia was higher, from Europe assembled. The results also showed that the genetic variation of accessions from Indian and Middle Eastern safflower diversity centers were relatively higher. ISSR is an effective and promising marker system for detecting genetic diversity among safflower and give some useful information on its phylogenic relationships.
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Acknowledgements
The authors thank Dr. Bradley of American Germplasm Resources Information Network (GRIN) for kindly providing 42 safflower accessions, Mr. Han Xinnian of Xinjiang Agricultural Academy of Science for providing Xinghong 1, Xinghong 4 and UC 26, Ms. Tang Li of Yaan Sanjiu Medicine Co. Ltd. for providing Chuanhong 1 and Jianyanghonghua accessions used in this experiment. And the experiment was accomplished in the lab of Gene Resource and Molecular Breeding in Sichuan Agricultural University. This work is financial supported by the Youth Science and Technology Foundation of Sichuan Province in China.
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Yang, YX., Wu, W., Zheng, YL. et al. Genetic diversity and relationships among safflower (Carthamus tinctorius L.) analyzed by inter-simple sequence repeats (ISSRs). Genet Resour Crop Evol 54, 1043–1051 (2007). https://doi.org/10.1007/s10722-006-9192-3
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DOI: https://doi.org/10.1007/s10722-006-9192-3