Abstract
The present study evaluated the effects of 100 and 500 μM arsenate (Na2HAsO4) on pigment composition and photosynthesis in Hydrilla verticillata (L.f.) Royle. Arsenic accumulation increased in concentration and duration dependent manner. The maximum accumulation [568 μg(As) g−1(d.m.)] was observed at 500 μM concentration and 96-h exposure. This concentration led to a significant decline in chlorophyll a content and PS II efficiency during the whole experiment, and in chlorophyll b and carotenoids after 96 h, but no significant changes in photosynthetic pigments were noticed at 100 μM arsenate. Net photosynthetic rate, electron transport rate, and water use efficiency declined whereas transpiration rate increased, and stomatal conductance and photochemical quenching did not show any effect or increased. The content of reactive oxygen species increased and content of reduced ascorbate declined at 500 μM arsenate in comparison to the control.
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Abbreviations
- ASC:
-
ascorbate
- Car:
-
carotenoids
- Chl:
-
chlorophyll
- ci :
-
substomatal CO2 concentration
- E:
-
transpiration rate
- ETR:
-
electron transport rate
- Fv′/Fm′:
-
efficiency of excitation energy capture by PS II
- gs :
-
stomatal conductance
- NPQ:
-
nonphotochemical quenching
- φPSII :
-
photosystem II efficienty
- PN :
-
net photosynthetic rate
- PN/E :
-
water use efficiency
- PQ:
-
photochemical quenching
References
Ahsan, N., Lee, D.G., Kim, K.H., Alam, I., Lee, S.H., Lee, K.W., Lee, H., Lee, B.H.: Analysis of arsenic stress-induced differentially expressed proteins in rice leaves by twodimensional gel electrophoresis coupled with mass spectrometry. — Chemosphere 78: 224–231, 2010.
Chaitanya, K.S.K., Naithani, S.C.: Role of superoxide, lipid peroxidation and superoxide dismutase in membrane perturbation during loss of viability in seeds of Shorea robusta Gaertn. f. — New Phytol. 26: 623–627, 1994.
Duman, F., Ozturk, F., Aydin, Z.: Biological responses of duckweed (Lemna minor L.) exposed to the inorganic arsenic species As(III) and As(V): effects of concentration and duration of exposure. — Ecotoxicology 19: 983–993, 2010.
Finnegan, P.M., Chen, W.: Arsenic toxicity: the effects on plant metabolism. — Front. Physiol. 3: 182, 2012.
Gillespie, K.M., Ainsworth, E.A.: Measurement of reduced, oxidized and total ascorbate content in plants. — Nature Prot. 2: 871–874, 2007.
Irtelli, B., Navari-Izzo, F.: Uptake kinetics of different arsenic species by Brassica carinata. — Plant Soil 303: 105–113, 2008.
Jain, M., Gadre, R.P.: Effect of As on chlorophyll and protein contents and enzymatic activities in greening maize tissues. — Water Air Soil Pollut. 93: 109–115, 1997.
Jain, M., Gadre, R.P.: Inhibition of 5-amino levulinic acid dehydratase activity by arsenic in excised etiolated maize leaf segments during greening. — J. Plant Physiol. 161: 251–255, 2004.
Li, W.-X., Chen, T.-B., Huang, Z.-C., Lei, M., Liao, X.-Y.: Effect of arsenic on chloroplast ultrastructure and calcium distribution in arsenic hyperaccumulator Pteris vittata L. — Chemosphere 62: 803–809, 2006.
Lichtenthaler, H.K., Buschmann, C.: Extraction of photosynthetic tissues: chlorophylls and carotenoids. — In: Chanda, V. (ed.): Current Protocols in Food Analytical Chemistry. Pp. F4.2.1–F4.2.6. John Wiley and Sons, New York 2001a.
Lichtenthaler, H.K., Buschmann, C.: Chlorophylls and carotenoids: measurement and characterization by UV-VIS spectroscopy. — In: Chanda, V. (ed.): Current Protocols in Food Analytical Chemistry. Pp. F4.3.1–F4.3.8. John Wiley and Sons, New York 2001b.
Liu, K.L., Shen, L., Wang, J.Q., Sheng, J.P.: Rapid inactivation of chloroplastic ascorbate peroxidase is responsible for oxidative modification to Rubisco in tomato (Lycopersicon esculentum) under cadmium stress. — J. Integr. Plant Biol. 50: 415–426, 2008.
Luo, H.-B., Ma, L., Xi, H.-F., Duan, W., Li, S.-H., Loescher, W., Wang, J.-F., Wang, L.-J.: Photosynthetic responses to heat treatments at different temperatures and following recovery in grapevine (Vitis amurensis L.) leaves. — PLOS One 6: e23033, 2011.
Milivojevic, D.B., Nikolic, B.R., Drinic, G.: Effects of arsenic on phosphorus content in different organs and chlorophyll fluorescence in primary leaves of soybean. — Biol. Plant. 50: 149–151, 2006.
Nwugo, C.C., Huerta, A.J.: Silicon-induced cadmium resistance in rice (Oryza sativa). — J. Plant Nutr. Soil Sci. 171: 841–848, 2008.
Rahman, M.A., Hasegawa, H., Rahman, M.M., Islam, M.N., Miah, M.A.M., Tasmen, A.: Effect of arsenic on photosynthesis, growth and yield of five widely cultivated rice (Oryza sativa L.) varieties in Bangladesh. — Chemosphere 67: 1072–1079, 2007.
Srivastava, S., D’souza, S.F.: Increasing sulfur supply enhances tolerance to arsenic and its accumulation in Hydrilla verticillata (L.f.) Royle. — Environ. Sci. Technol. 43: 6308–6313, 2009.
Srivastava, S., D’souza, S.F.: Effect of variable sulfur supply on arsenic tolerance and antioxidant responses in Hydrilla verticillata (L.f.) Royle. — Ecotoxicol. Environ. Safety 73: 1314–1322, 2010.
Srivastava, S., Mishra, S., Tripathi, R.D., Dwivedi, S., Trivedi, P.K., Tandon, P.K.: Phytochelatins and antioxidant systems respond differentially during arsenite and arsenate stress in Hydrilla verticillata (L.f.) Royle. — Environ. Sci. Technol. 41: 2930–2936, 2007.
Srivastava, S., Suprasanna, P., D’souza, S.F.: Redox state and energetic equilibrium determine the magnitude of stress in Hydrilla verticillata upon exposure to arsenate. — Protoplasma 248: 805–815, 2011.
Srivastava, S., Suprasanna, P., D’souza, S.F.: Mechanisms of arsenic tolerance and detoxification in plants and their application in transgenic technology: A critical appraisal. — Int. J. Phytoremed. 14: 506–517, 2012.
Stoeva, N., Berova, M., Zlatev, Z.: Effect of arsenic on some physiological parameters in bean plants. — Biol. Plant. 49: 293–296, 2005.
Yabuta, Y., Mieda, T., Rapolu, M., Nakamura, A., Motoki, T., Maruta, T., Yoshimura, K., Ishikawa, T., Shigeoka, S.: Light regulation of ascorbate biosynthesis is dependent on the photosynthetic electron transport chain but independent of sugars in Arabidopsis. — J. exp. Bot. 58: 2661–2671, 2007.
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Srivastava, S., Srivastava, A.K., Singh, B. et al. The effect of arsenic on pigment composition and photosynthesis in Hydrilla verticillata . Biol Plant 57, 385–389 (2013). https://doi.org/10.1007/s10535-012-0288-7
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DOI: https://doi.org/10.1007/s10535-012-0288-7