Abstract
The objective of the study was to investigate the effect of different arsenic concentrations on some physiological parameters of bean (Phaseolus vulgaris L.) cultivars Plovdiv 10 and Prelom in the early growth phases. Seedlings, grown in sand with Hoagland-Arnon nutrient solution in a climatic box, were treated with 0, 2, 5 mg(As) dm−3 as Na3AsO4 (pH 5.5). After 5 d of As treatment, the changes in leaf gas-exchange, water potential, chlorophyll and protein contents, peroxidase activity and lipid peroxidation in roots were recorded. Physiological analysis showed a minor negative effect of arsenic at concentration 2 mg(As) dm−3, but at the higher dosage of 5 mg(As) dm−3 growth, leaf gas-exchange, water potential, protein content and biomass accumulation were reduced in both cultivars. The peroxidase activity and lipid peroxidation increased considerably at 5 mg(As) dm−3, which is a typical reaction of the plants to a presence of oxidative stress.
Article PDF
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
Abbreviations
- E:
-
transpiration rate
- gs :
-
stomatal conductance
- LP:
-
lipid peroxidation
- MDA:
-
malondialdehyde
- PAR:
-
photosynthetically active radiation
- PN :
-
net photosynthetic rate
- POD:
-
peroxidase
- ROS:
-
reactive oxygen species
- RWC:
-
relative water content
- SOD:
-
superoxide dismutase
- TBA:
-
thiobarbituric acid
- Ψ:
-
water potential
References
Adrino, D.C.: Trace Elements in the Terrestrial Environment.-Springer-Verlag, New York 1986.
Cakmak, I., Horst, W.J.: Effect of aluminum on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tips of soybean (Glycine max).-Physiol. Plant. 83: 463–468, 1991.
Clijsrers, H., Van Assche, F.: Inhibition of photosynthesis by heavy metals.-Photosynth. Res. 7: 31–40, 1985.
De Vos, C.H., Vooijs, R., Schat, H., Ernst, W.H.: Cooper-induced damage to the permeability barrier in roots of Silene cucubalus.-J. Plant Physiol. 135: 165–169, 1989.
Elstner, E.F., Wagner, G.A., Schutz, W.: Activated oxygen in green plants in relation to stress situations.-Curr. Topics Plant Biochem. Physiol. 7: 159–187, 1988.
Flora, S.: Arsenic-induced oxidative stress and its reversibility following combined administration of N-acetylcysteine and meso 2,3-dimercaptosuccinic acid in rats.-Clinical exp. Pharmacol. Physiol. 26: 865–869, 1999.
Hartley-Whitaker, J., Ainsworth, G., Meharg, A.: Copper-and arsenic-induced oxidative stress in Holcus lanatus L. clones with differential sensitivity.-Plant Cell Environ. 24: 713–722, 2001.
Heath, R., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.-Arch. Biochem. Biophys. 125: 189–198, 1968.
Herzog, V., Fahimi, H.: A new sensitive colorimetric assay for peroxidase using 3,3′-diaminobenzidine as hydrogen donor.-Anal. Biochem. 55: 554–562, 1973.
Journet, E.P., Bligny, R., Douce, R.: Biochemical changes during sucrose deprivation in higher plant cells.-J. biol. Chem. 261: 3193–3199, 1986.
Lee, K., Cunningham, B., Paulsen, G., Liang, G., Moore R.: Effect of cadmium on respiration rate and activities of several enzymes in soya been seedlings.-Physiol. Plant. 36: 4–6, 1976.
Lichtenthaler, H., Wellburn, A.: Determination of total carotenoids and chlorophyll a and b of leaf extracts in different solvents.-Biochem. Soc. Trans. 603: 591–592, 1983.
Lowry, O.H., Rosenbough, N.Z., Farr, A.L., Randall, R.J.: Protein measurements with Folin phenol reagent.-J. biol. Chem. 193: 265–275, 1951.
Lynn, S., Shiung, J., Gurr, J., Yan, K.: Arsenic stimulates poly (adp-ribosylation) by generation of nitric oxide.-Free Radical Biol. Med. 24: 442–449, 1998.
Marin, A.R., Masscheleyn, P.H., Patrik, J.: The influence of chemical form and concentration of arsenic on rice growth and tissue arsenic concentration.-Plant Soil 139: 175–183, 1992.
Merakchiyska, M., Yordanov, I.: [Influence of some heavy metals on the growth, content of plastid pigments, and the photosynthetic activity in bean plants.]-In: Scientific Conference on Botany. Pp. 848–851. BASC, Sofia 1983. [In Bulg.]
Miteva, E., Merakchiyska, M.: Response of chloroplasts and photosynthetic mechanism of bean plants to excess arsenic in soil.-Bulg. J. agr. Sci. 8: 151–156, 2002.
Miteva, E., Peycheva, S.: Arsenic accumulation and effect on peroxidase activity in green bean and tomatoes.-Bulg. J. agr. Sci. 5: 737–740, 1999.
Mocquot, B., Van Gronsveld, J., Clijsters, H., Mench, M.: Copper toxicity in young maize (Zea mays L.) plants: effect on growth, mineral and chlorophyll contents and enzyme activities.-Plant Soil 182: 287–300, 1996.
Moustakas, M., Lanaras, T., Symeonidis, L., Kartaglis, S.: Growth and some photosynthetic characteristics of field grown Avena sativa under copper and lead stress.-Photosynthetica 30: 389–396, 1994.
Mylona, P.V., Polidoros, A.N., Scandalios, J.G.: Modulation of antioxidant responses by arsenic in maize.-Free Radical Biol. Med. 25: 576–585, 1998.
Stepanok, V.V.: [The effect of arsenic on the yield and elemental composition of agricultural crops.]-Agrokhimiya 12: 57–63, 1998. [In Russ.].
Stoeva, N., Berova, M., Zlatev, Z.: Physiological response of maize to arsen contamination.-Biol. Plant. 47: 449–452, 2003/4.
Stoeva, N., Bineva, Tz.: Oxidative changes and photosynthesis in oat plants grown in As-contaminated soil.-Bulg. J. Plant Physiol. 29: 87–95, 2003.
Van Assche, F., Clijsters, H.: Effect of metals on enzyme activity in plants.-Plant Cell Environ. 13: 195–206, 1990.
Wells, B.R., Gilmor, J.: Sterility in rice cultivars as influenced by MSMA rate and water management.-Agron. J. 69: 451–454, 1997.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Stoeva, N., Berova, M. & Zlatev, Z. Effect of arsenic on some physiological parameters in bean plants. Biol Plant 49, 293–296 (2005). https://doi.org/10.1007/s10535-005-3296-z
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10535-005-3296-z