Abstract
Although macauba palm (Acrocomia aculeata) is a palm native to neotropics with wide distribution in Brazil and has significant potential for biofuel production, little is known regarding its variability and adaptability to environment. The aim of this study was to investigate the association of macauba palm phenotypes to soil attributes and other environmental factors. Macauba palms from 18 locations of three geomorphological provinces from the state of São Paulo, Brazil, were evaluated regarding chemical, physical and morphological soil attributes and plant morphological attributes. Data were analyzed by uni- and multivariate statistics. Univariate statistical analysis showed that there is great variability of botanical components due to soil fertility, particle size distribution and drainage and to atmospheric climate conditions. Principal component analysis identified the botanical variables fruit weight and size and palm tree circumference at chest height, and the soil variables organic matter of A horizon contents of sand and clay, and exchangeable calcium, manganese, copper, potassium and phosphorus from subsurface horizons as those with the greatest contribution in distinguishing the studied environments. Redundancy analysis identified total sand—inversely associated to water retention—pH—directly influenced by exchangeable bases—and micronutrients Zn and Mn from subsurface horizons, together with soil organic matter from A horizon, as those most associated to the botanical variables fruit weight and size. These associations show that soil fertility and particle size distribution are determining factors for the productive potential of macauba palm.
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Introduction
Palms (Arecaceae) constitute a group of plants common in tropical forests that can show morphological, phenological and genetic divergences among closely related species along resource gradients (Henderson et al. 1995; Svenning 2001; Savolainen et al. 2006).
Macauba palm (Acrocomia aculeata (Jacq.) Lodd ex Mart.) is a perennial fruit-bearing palm tree, with spines, whose stipe may reach 15 m height with 20–30 cm diameter (Lorenzi 1992), native of the savannas and open forests, distributed throughout tropical and subtropical Americas (Henderson et al. 1995). This species presents phenotypic plasticity or ability to express different phenotypes under various environmental conditions (Schlichting 1986). The macauba palm has mixed mating system (Abreu et al. 2012) with extensive genetic variability within and among natural populations (Nucci et al. 2008) expressed on the degree of germination and morpho-agronomic characters (Berton et al. 2013).
Currently, the macauba palm has drawn interest due to the possibility of its use for biofuel production. Its fruits show great potential for oil production, being able to produce over 4000 L of vegetable oil per hectare (Silva 1994; Colombo et al. 2017; Evaristo et al. 2016). The residual cake of the oil extraction from the fruit is used in animal feed and may also be employed as an effective filter in the adsorption of dye waste in chemical industrial effluents (Vieira et al. 2012). Nowadays, it is used in popular medicine, as food, and in craftwork and cosmetics, among other applications (Lima et al. 2003). There are pilot projects involving macauba palm in agroforestry systems in Brazil and planting initiatives of this species for recovery of degraded areas.
The macauba palm occurs spontaneously throughout nearly the whole Brazilian territory. However, its most dense populations are located in Minas Gerais, São Paulo, Goiás, Mato Grosso and Mato Grosso do Sul, being widespread in Cerrado (a tropical savanna typical of Brazil) areas (Bondar 1964; Silva 1994; Henderson et al. 1995; Scariot et al. 1991, 1995; Lima et al. 2003).
Despite its wide geographical distribution, little is known regarding macauba palm adaptability to environmental conditions such as those of soils, climate and vegetation formations associated to its populations within the Brazilian territory. Moreover, little is known on the association between environmental conditions and phenotypic characteristics of its populations. Nevertheless, the combination of ecological plasticity, favorable physiological attributes such as high photosynthetic capacity, and high productivity should give macauba palm great capacity of adaptation to its different natural occurring environments (Pires et al. 2013), taking Fisher’s (1930) concept of adaptation, as the movement of a population towards a phenotype that best fits the present environment.
Soil is a major determinant of the composition and functioning of terrestrial ecosystems (Lambers et al. 2008). On a particular landform, the distributional pattern of plant species has been related to plant strategies, tolerance for severe stress and disturbance gradients, and biotic interactions (Levine 2000). In that sense, macauba palm is considered one of the most conspicuous palm species in Brazil, as it grows spontaneously in large populations either in degraded or intact areas and is well-adapted to different ecosystems (Motta et al. 2002; Ratter et al. 2003). Their spontaneous distribution follows naturally high fertility soils and native forest vegetation, evidencing that despite the species pioneer behavior, it avoids extremes of nutrient and water deficiencies (Motta et al. 2002), as illustrated in Fig. 1.
The present study seeks to investigate the association of macauba palm phenotypes to environmental sources of variation as factors of adaptation.
Materials and methods
Eighteen locations with native macauba palm populations were previously field-identified and selected according to their geographic distribution along the state of São Paulo (Table 1). Those locations represented three geomorphological provinces of the state of São Paulo: Basaltic Cuestas (Cuestas Basálticas), Peripheral Depression (Depressão Periférica) and Occidental Plateau (Planalto Ocidental) (Ponçano et al. 1981). Some of those palm trees occur under distinct environments regarding geological formation, relief, native vegetation associated to them, and climate, among other environmental factors, besides differing in botanical characteristics, what reinforces the need to study them separately. Soil collection was carried out by composite sampling with an auger under the projection of palm trees canopy, with samples composed of five subsamples in the years of 2008 and 2009. Soils were collected at two layers: 0–20 and 20–50 cm depth, based on the assumption that the soil profile down to 50 cm is the most important for the development of macauba palm. Nevertheless, soils were observed down to the depth of 1 m to identify other limiting soil factors and to classify soils. Soil classification at each sampling location was made according to the U.S. Soil Taxonomy (Soil Survey Staff 2010). Morphological attributes used for soil characterization and classification were color (Munsell Soil Color chart), particle-size distribution (PSD) and consistency when moist, besides soil depth and drainage (Soil Survey Division Staff 1993), in addition to epipedon and soil subsurface diagnostic horizons (Soil Survey Staff 2010).
Soil particle size distribution (PSD) analysis was carried out by the densimeter (modified Bouyoucos) method (Camargo et al. 1986). Soil chemical determinations for fertility purposes were pH in CaCl2, organic matter (SOM), potential acidity (H + Al), and exchangeable potassium (K), phosphorus (P), calcium (Ca), magnesium (Mg), boron (B), iron (Fe), manganese (Mn), zinc (Zn) and copper (Cu) (Raij et al. 2001). After chemical analysis, cation exchange capacity (CEC), sum of bases (SB) and base saturation (V%) were calculated.
Collection of botanical material was carried out in 90 plants, five productive plants for each palm tree population, from February to April, 2009. Botanical variables measured and observed in the field were circumference at breast height (CBH), plant height, number of bunches in production, dry bunches and total bunches. From each palm tree, 20 fruits from the same bunch were collected with the aid of a ladder and a pruner. Fruit weight, length and width measurements were taken in the laboratory using analytical balance and Starrett series 799-6/150 digital caliper rule.
Plant morphology data were subjected to analysis of variance, and mean values were compared by Tukey test at 5% significance through the SISVAR 4.6 program (Ferreira 2003) for the purpose of evaluating how these variables behave in relation to the environmental sources of variation (geomorphological province, climate, drainage, soil nutrients and acidity, soil particle size distribution and lithology). Number of bunches in production was standardized according to the equation: [Square root of y + 0.5 − Square root (y + 0.5)].
Multivariate statistical analysis was used to evaluate soil-vegetation relationships. Environmental and plant morphological data were initially analyzed by the principal component analysis (PCA) ordering method, and then by redundancy analysis (RDA). Data matrix for PCA analysis was composed by n locations and m descriptors of the environment and by plant morphology descriptors. There were seven plant morphology descriptors and 30 environmental descriptors, including soil chemical and physical attributes at the two sampled layers (0–20 and 20–50 cm) per palm tree location. Complementary variables, such as silt, that is the fine earth (clay + silt + sand) minus “clay + sand” and sum of bases (Ca + Mg + K + Na) as well as base saturation were eliminated for being mutually associated or supplementary. This analysis allowed evaluation of variable variances all together, thus selecting 13 environmental soil variables (physical and chemical) from the 20 to 50 cm layer, two variables from the 0 to 20 cm layer, and seven variables of plant morphology to participate in the final data matrix. As required by the RDA, two data matrices were organized, one considering plant morphological data, and another, environmental data consisting of those 22 variables previously selected by PCA. For the RDA to be statistically valid, the number of m descriptors (botanical and environmental) must be less than the number of n locations. Monte Carlo permutation test (Ter Braak 1988) was used to evaluate the level of significance of the RDA ordination axes. Multivariate statistics were processed by FITOPAC 1.6 software (Shepherd 2006).
Results and discussion
Macauba palm trees were identified in areas corresponding to two Koeppen climatic types: Cwa, subtropical, with rainy summers, dry winters, mean temperature of the hottest month above 22 °C and of the coldest month below 18 °C; and Aw, tropical rainy, with dry winter, mean temperatures above 18 °C in the coldest month and above 22 °C in the most warm month. Considering all studied locations, mean annual temperatures range from 15 to 28 °C, coincident with the annual minimum and maximum reported by Motta et al. (2002). Land use associated to macauba palm populations shows large diversity, but macauba individuals under a more dense plant cover, such as forest, can hardly be found, suggesting that the species adapts more easily to environments of lower competition for light and nutrients. Considering slope and their relative position on landscape, macauba palm trees are hardly found on hill summits, but frequently found on hillsides and lowland, which is in agreement with observations of Motta et al. (2002) in three geographical regions of the Minas Gerais state, Brazil, where the palm occurs at positions from midslope to toeslope.
From 18 locations, 10 had soils with base saturation (V) ≥ 50% in the A horizon and eight have V < 50% on the A horizon. Of the 18 sampled subsurface horizons eight had a high fertility status (V ≥ 50%) and 10 had a low fertility (V < 50%) (Table 2). The greatest V values (51 to 70%) were found in soils from the Basaltic Cuestas province, what is in the range of moderate base saturation (Raij et al. 1996). Soil fertility was considered mainly through evaluation of the 20–50 cm layer (B or C horizon), layer less influenced by land management than those superficial. The soil’s first 50-cm depth was considered the most important layer for development of macauba palm roots. Five locations had soils of very high acidity (Raij et al. 1996), located in the municipalities of São Simão, São Pedro do Turvo and Piquerobi; eight soils had high acidity, from Jaguariúna, Campinas, Barbosa and Teodoro Sampaio; four had medium acidity and were located at Campinas and Dourado; and only one was identified with low acidity, occurring in Santa Rosa do Viterbo. This shows macauba palm adaptability to a large range of soil acidity levels. Soil macronutrient content classes, as described by Raij et al. (1996), were different depending on the geomorphological region, with prevalence of low to medium macronutrient contents. Soils showed high levels of micronutrients in three geomorphological provinces. Soil B horizon particle-size distribution was mostly loamy. Although drainage of the sampled locations showed large variation (Table 3), those occurrences agree with the findings of Markley (1956) and Martin and Guichard (1979), which indicated limited distribution of macauba palm in areas of deficient drainage.
Results of univariate statistics (Table 4) showed that, except for CBH, studied botanical variables differed significantly among locations, showing a great deal of variability among macauba palm trees. This may be due to genetic variability of plants from different locations, to environmental factors variability among the three geomorphological provinces of the state of São Paulo or to the combination of these factors. Bunches in production, botanical variable related to the productive potential of the palm tree, exhibited the greatest mean values at the Dourado 1 location. Furthermore, in that location, the fruits were relatively larger and heavier. Botanical variables CBH and fruit size and weight were affected by climatic types, with greater values of these three variables under the more mild Cwa climate, than under the hotter Aw climate. Plants from Peripheral Depression and Basaltic Cuestas geomorphological provinces populations were taller, had greater CBHs and larger and heavier fruits than those of the Occidental Plateau.
Poor to somewhat poor drainage was also responsible for significant reduction in fruit weight and size, CBH and bunches in production, whereas plant height was not affected by soil drainage. Fruit size and weight and plant height showed greater values on soils developed from granitic rocks, whereas greater number of bunches in production was found on soils developed from basic rocks. Association of botanical variables to rock type distribution may be consequence of the influence of this factor for soil formation or of the broad spatial distribution of these geological formations, which cannot be dissociated from climatic, topographic or plant genetics variations. The analysis showed there is large plasticity on botanical components of macauba palm among the populations. Possible environmental causes are soil fertility and soil water availability, determined by particle size distribution, drainage and climate, with this last factor being differentiated among studied locations especially by small differences in temperatures, which are milder in the Cwa, and in the length of the dry season, longer in the Aw.
Soil and plant data matrix were initially analyzed by PCA, used as a selective and exploratory method, and consisted of 18 sampled locations (plant populations) and 37 variables, where 30 were soil variables of the depths 0–20 and 20–50 cm, and seven plant variables. Few available data of land use history from each studied site and the fact that subsurface soil layers carry more stable attributes for perennial plants and are less influenced by those non-controlled practices were considered, together with PCA results, to eliminate all environmental variables from A horizon, except for SOM (soil organic matter) and H + Al (potential acidity). For further analysis, all soil variables from B horizon plus SOM and H + Al from A horizon together with all plant variables were kept for final analysis, remaining a total of 22 variables. Variables were identified by the symbols “-A” for the A horizon and “-B” for the B horizon. All selected variables showed correlations greater than 0.2 with axes 1 and 2.
The new PCA analysis showed CBH, total sand, clay, SOM, pH, K, Ca, CEC, Cu, and Mn had greater correlation to axis 1, which expressed 37.91% of the total data variance. Plant height, fruit weight, fruit size, number of dry bunches, P, Mg, H + Al, B, Fe and Zn were most correlated to axis 2, which represented 13.09% of data total variance. Weight and size of fruits and P correlated well to both ordination axes.
PCA plot (Fig. 2) showed that distribution of macauba palm follows very closely the direction of both pH and clay vectors. The trees on soils with high pH (Viterbo 2; Dourado 1, 2 and 3; Campinas CEC 3) follow closely the pH vector, a vector with the highest score with PCA axis 1. The vectors of the basic cations K, Ca and Mg showed a similar tendency than that of the pH vector and are thus associated to the same macaúba palm populations. On those five populations soils were developed from basic rocks (basalt and diabase). Very similar association is observed between those populations and the clay vector, another vector with high score with the PCA axis 1. In the very opposite direction the total sand vector is represented, that is closely associated to Piquerobi 1, 2 and 3, Porto X, Morro do Diabo and São Simão, all of those with soils derived from sandstones, loamy to sandy soils. Therefore, the studied macauba palm populations show a very clear gradient regarding soil fertility and particle-size distribution.
An almost inverse relationship was observed between total sand and morphological variables that indicated plant vigor (CBH, plant height, fruit size and number of bunches in production, dry bunches and total bunches). Since macauba palm populations (Porto X, Morro do Diabo and Piquerobi 1, 2 and 3) more opposed to those botanical variables, besides being more sandy, are also under a Aw climate, with warmer dry winter than populations under Cwa, one can associate their less favorable botanical attributes to a greater water deficit, determined by soil and climate, not neglecting the fact that soil of those sites have also lower nutrient availability.
Redundancy Analysis (RDA) was carried out using two data matrices, one of plant morphological data (7), and another of environmental data (15). Due to the high correlations between soil P and soil Ca, the program FITOPAC 1.6 (Shepherd 2006) automatically eliminated the Ca variable as redundant. Thus, the environmental data matrix was composed of 14 variables. In RDA, the first two axes explained almost 34% of total data variance, 22.59% by axis 1 and 11.17% by axis 2 (Fig. 3). This value is considered excellent in studies of ecological and environmental data due to the high number of variables that may be influencing an observation. Therefore, environmental variables of this study can explain quite well botanical attributes. Monte Carlo permutation test (Ter Braak 1988), which evaluates the level of significance of RDA ordination axes, showed significance at the level of 1% for the first three ordination axes.
In general, RDA biplot was more effective than PCA in performing ordination as function of geographical location of macauba palm trees, especially placing populations from Occidental Plateau on the plot left side, far from populations from Basaltic Cuestas or from peripheral depression (Fig. 3). RDA also captured better the relationship between environmental variables and macauba palm diversity. SOM-A, pH-B, Mn-B, clay-B, CEC-B, and Zn-B, on one end, and total sand-B, on the other end, were the environmental variables most responsible for ordering macauba palm populations on axis 1. Nevertheless, all environmental variables, except Fe-B and Mg-B, showed high correlation to axis 1 if compared to axis 2. RDA analysis confirmed evidences observed on PCA that botanical attributes that relate to favorable agronomic features are strongly influenced by soil chemical (fertility) and physical (particle size) attributes.
The high SOM-A contents on the Peripheral Depression study sites (Red, Estância Regina and CC3) (Fig. 3) is supposed to be due to the very dense macauba palm populations in those three sites, with little direct sunlight, favoring plant residues addition and SOM accumulation. Soil organic matter (SOM) is an attribute that favors plant growth due to increasing soil chemical fertility, promoting good soil physical conditions and offering a more adequate environment for soil organisms. SOM-A being the vector with the highest score with RDA axis 1 shows that macauba palm botanical features responded positively to those soil conditions and corroborated observations of Rocha (1946) and Lorenzi (1992) that this palm adapts more easily to more fertile soils.
Following SOM-A and pH-B, variables that most correlated to axis 1 were total sand-B, Mn-B, clay-B, Zn-B, CEC-B, K-B, Cu-B, B-B, P-B, and H + Al-A, what shows that more than being responsible for macauba palm ordering by RDA, these soil attributes do contribute for determining changes on macauba palm botanical features, at least on those included on the RDA botanical matrix.
Botanical variables that correlated most to axis 1 were fruit size and weight (Fig. 4). Total of bunches, bunches in production and plant height correlated better to RDA ordination axis 2, which was not associated to any environmental attribute. Dry bunches and CBH did not show high correlation to any ordination axes. Fruit size and weight were the botanical variables that explained most of data’s total variance and are thereby associated to landscape stratification (with strong distribution pattern along the same RDA axis), represented in the RDA by soil fertility and particle-size attributes. The remaining botanical features may be related to plant age (height), phenological seasonality (total of bunches; bunches in production) or biannuality, or determined more strongly by plant intrinsic features, such as its genetics.
Conclusions
The studied sites show large variability of environmental factors, allowing stratification of macauba palm trees by different groups of variables. Soil chemical fertility—A horizon soil organic matter and B horizon pH, Ca, Mn, Cu, K and P—particle-size distribution—B horizon clay and sand—soil drainage and atmospheric climate are non-biotic factors most contributing for stratifying macauba palm in regard to its environment of occurrence.
Size and weight of macauba palm fruits, botanical features intended for agronomic purposes, are the most correlated to environmental factors, indicating that they may respond to a change in those non-biotic factors under agronomic management.
Therefore, in a prospecting approach, the study shows chemical fertility, represented by macro and micronutrients, particle-size distribution and degree of soil drainage as site-specific factors that can determine productive potential of macauba palm.
Number of dry bunches and circumference of the stem at chest height correlate very poor to all variables of the non-biotic environment.
Total bunches of macauba palm, bunches in production and plant height do not correlate to any of the studied environmental attributes, but their pattern of variation suggests that other factors such as plant age, genetics, phenology or biannuality may influence them more strongly.
References
Abreu AG, Priolli RHG, Azevedo Filho JA, Nucci SM, Zuchi MI, Coelho RM, Colombo CA (2012) The genetic structure and mating system of Acrocomia aculeata (Arecaceae). Genet Mol Biol 35:119–121
Berton LHC, Azevedo Filho JA, Siqueira WJ, Colombo CA (2013) Seed germination and estimates of genetic parameters of promising macaw palm (Acrocomia aculeata) progenies for biofuel production. Ind Crop Prod 51:258–266
Bondar G (1964) Palmeiras do Brasil. Instituto de Botânica, São Paulo
Camargo OA de, Moniz AC, Jorge JA, Valadares JMAS (1986) Métodos de análise química, mineralógica e física de solos. Instituto Agronômico-IAC, Campinas-São Paulo (Boletim Técnico 106)
Colombo CA, Berton LHC, Diaz BG, Ferrari RA (2017) Macauba: a promising tropical palm for the production of vegetable oil. OCL. https://doi.org/10.1051/ocl/2017038
Evaristo AB, Grossi JAS, Carneiro ACO, Pimentel LD, Motoike SY, Kuki KN (2016) Actual and putative potentials of macauba palm as feedstock for solid biofuel production from residues. Biomass Bioenergy 85:18–24
Ferreira DF (2003) SISVAR 4.6 sistemas de análises estatísticas. Universidade Federal de Lavras, Lavras
Fisher RA (1930) The genetical theory of natural selection. Clarendon Press, Oxford
Henderson A, Galeano G, Bernal R (1995) Field guide to the palms of the Americas. Princepton University, New Jersey, pp 166–167
Lambers H, Chapin FS, Pons TL (2008) Plant physiological ecology. Springer, New York
Levine JM (2000) Complex interactions in a streamside plant community. Ecology 81:3431–3444
Lima ES, Felfifili JM, Marimon BS, Scariot A (2003) Diversidade, estrutura e distribuição espacial de palmeiras em um cerrado sensu stricto no Brasil central. Rev Bras Bot 26:361–370
Lorenzi H (1992) Árvores brasileiras: manual de identificação e cultivo de plantas arbóreas nativas do Brasil. Editora Plantarum, Nova Odessa, SP
Markley KS (1956) Mobocayá or Paraguay coco palm: an important source of oil. Econ Bot 10:3–32
Martin G, Guichard PH (1979) A propos de quatre palmiers spontanes d’Amerique Latine. Oleaginêux 34:375–384
Motta PEF, Curi N, Oliveira-Filho AT, Gomes JBV (2002) Ocorrência da macaúba em Minas Gerais: relação com atributos climáticos, pedológicos e vegetacionais. Pesq Agropec Bras 37:1023–1031
Nucci SM, Azevedo Filho JA, Colombo CA, Priolli RH, Zucchi MI (2008) Development and characterization of microsatellites markes from the macaw. Mol Ecol Resour 8:224–226
Pires TP, dos Santos Souza E, Kuki KN, Motoike SY (2013) Ecophysiological tratits of the macaw palm: a contribution towards the domestication of a novel oil crop. Ind Crop Prod 44:200–210
Ponçano W, Carneiro CDR, Bistrichi CA, Almeida FFM, Prandini FL (1981) Mapa geomorfológico do estado de São Paulo (Escala 1:1.000.000). V1 e V2 (Mapa e Nota Explicativa). Instituto de Pesquisas Tecnológicas, São Paulo (IPT Monografias, 5)
Raij BV, Cantarella H, Quaggio JA, Furlani AMC (1996) Recomendações de adubação e calagem para o estado de São Paulo. 2a ed, Instituto Agronomico-IAC, Campinas-São Paulo (Boletim Técnico No 100)
Raij BV, Andrade JC, Cantarella H, Quaggio JA (2001) Análise Química para fertilidade de solos tropicais, 1a edn. Instituto Agronomico-IAC, Campinas-São Paulo
Ratter JA, Bridgewater S, Ribeiro JF (2003) Analysis of the floristic composition of the Brazilian cerrado vegetation III: comparison of the woody vegetation of 376 areas. J Bot 60:57–109
Rocha O (1946) O coco macaúba. Rev Agric 21:345–358
Savolainen V, Anstett MC, Lexer C, Hutton I, Clarkson JJ, Norup MV, Powell MP, Springate D, Salamin N, Baker WJ (2006) Sympatric speciation in palms on an oceanic island. Nature 441:210–213
Scariot A, Lleras E, Hay JD (1991) Reproductive biology of the palm Acrocomia aculeata in Central Brazil. Biotropica 23:12–22
Scariot A, Lleras E, Hay JD (1995) Flowering and fruiting phonologies of the palm Acrocomia aculeata: patterns and consequences. Biotropica 27:168–173
Schlichting CD (1986) The evolution of phenotypic plasticity in plants. Annu Rev Ecol Syst 17:667–693
Shepherd GJ (2006) Fitopac 1.6: manual do usuário. Universidade Estadual de Campinas, Campinas, SP
Silva JC (1994) Macauba: fonte de matéria-prima para os setores alimentício, energético e industrial. Monografia. Universidade Federal de Viçosa, Brazil
Soil Survey Division Staff (1993) Soil survey manual, 2nd edn. United States Department of Agriculture, Washington, DC (USDA Handbook, 18)
Soil Survey Staff (2010) Keys to soil taxonomy, 11th edn. USDA-NRCS, Washington, DC
Svenning JC (2001) On the role of microenvironmental heterogeneity in the ecology and diversification of neotropical rain-forest palms (Arecaceae). Bot Rev 67:1–53
Ter Braak CJF (1988) Canoco-a FORTRAN program of canonical community ordination by (partial) (detrended) (canonical) correspondence analysis, principal components analysis and redundancy analysis (version 2.1). Statistics Department Wageningen, Wageningen (Technical report: LWA-88-02)
Vieira SS, Magriots ZM, Santos NAV, Cardoso MDG, Saczk AA (2012) Macauba palm (Acrocomia aculeata) cake from biodiesel processing: an efficient and low cost substrate for the adsorption of dyes. Chem Eng J 183:152–161
Acknowledgements
This work was funded by FAPESP (Fundação de Apoio à Pesquisa do Estado de São Paulo). CFC received a fellowship from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior). LHCB received a fellowship from FAPESP and CAC received a fellowship from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico). We acknowledge the statistical advise of GJ Shepherd.
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Coelho, R.M., da Costa, C.F., de Azevedo Filho, J.A. et al. Non-biotic factors determining plasticity of the prospective oil-rich macauba palm (Acrocomia aculeata). Agroforest Syst 93, 771–782 (2019). https://doi.org/10.1007/s10457-017-0173-7
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DOI: https://doi.org/10.1007/s10457-017-0173-7