Abstract
In Egypt, Trichogramma evanescens Westwood (TE) is extensively used in inundative releases against a number of lepidopterous pests of several crops. However, the wasp had not been collected from olive groves. Field trials on the use of commercially available TE against the olive moth, Prays oleae (Bern.) (OM) were carried out for three successive years (2002–2004). The objective of this study was to evaluate the efficacy of inundative releases of this wasp on damage reduction. The obtained results were encouraging since OM attacks were reduced by 42.9,71 and 69.9% and TE-treated trees yielded significantly bigger olive fruits by 10.5 and 12.5% than untreated trees in 2002 and 2004 olive seasons, respectively. However, parasitization levels indicated that the wasp is not well adapted to local environmental conditions of olive groves. The suggested measure to improve the quality of released wasps is to mass release of local wasps isolated during the present study, i.e., T. cordubensis Vargas and Cabello and T. euproctidis Girault.
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Introduction
The olive moth (OM), Prays oleae (Lepidoptera, Yponomeutidae) has recently become a serious pest in old and recently established and managed olive plantation, in Egypt, causing significant direct yield loss as well as aesthetic damage (Herz et al. 2005). The damage caused by this pest is at least 49–63% of production, which equates to 8–11 kg per tree (Ramos et al. 1998; Patanita and Mexia 2004). It is an important pest of olives in the Mediterranean basin (Lopes-Vellata 1999). The moth develops three generations have been reported per year. The first generation of moths appears in April. The females lay eggs on the flower buds. The newly hatched larvae live and feed within the buds and on the flowers at a later stage of their development. The second generation, emerges in early June causing the most important damage. The females oviposit on the small fruits close to the stem and the larvae bore into the stone of the olive fruit. When the larva completes its development in September and leaves the fruit, it causes spectacular fruit drop resulting in major crop losses. The third generation attacks the leaves; the emerging larvae mine in the olive leaves in autumn, hibernate as larvae and complete their development in early next spring (Pelekasis 1962).
The second and third generations of OM are relatively difficult to control with insecticides, because the larvae bore into the fruits and mine in the leaves immediately after hatching. Only the first generation is regarded as rather easy to control by spraying insecticides targeted at the larval stage. The characteristics of the feeding behaviour of OM larvae require the development of an integrated pest management approach because it is superfluous (Mazomenos et al. 1999).
Control of the OM is usually achieved by application of insecticides such as dimethoate, methidathion, endosulfan, cypermethrin, carbaryl and trichlorfon (Lopes-Vellata 1999). In general, the use of insecticides and other chemical treatments implies the risk of adverse ecological, toxicological and economic effects. Alternative techniques—mainly biological—include Bacillus thuringiensis (BT), the larvae the first generation (anthophagous) are highly susceptible to commercially available strains (Yamvrias et al. 1986). Of today, Trichogramma species (Hym.; Trichogramatidae) are the most widely used insect natural enemies in the world (Li 1994). The Trichogramma genus includes about 180 species of minute egg parasitoids of numerous insects, especially Lepidoptera (Pintureau 1990). The use of polyphagous egg parasitoids of the genus Trichogramma for the control of various species of orchard and field crop Lepidoptera has received much attention (Usun et al. 1996; Öztemiz and Kornosor 1999; Mills et al. 2000; Wright et al. 2001; Ulrichs and Mewis 2004). For instance, the efficacy of augmentative releases of T. dendrolimi and T. embryophagum against the codling moth, Cydia pomonella in apple orchards (Hassan et al., 1998); T. platneri against the avocado pest, Amorbia cuneana in avocado orchards (Honda 2005) and T. bourarachae, T. cordubensis and T. euproctidis against the jasmine moth, Palpita unionalis and the OM in olive orchards (Hegazi et al. 2007) was tested. The results suggested that releases of Trichogramma wasps could improve control of lepidopterous pests on orchards.
The egg parasitoid, Trichogramma evanescens Westwood is extensively used in inundative releases against a number of lepidopterous pests in Europe. Among the cereal crops, successful attempts to control the European corn borer, Ostrinia nubilalis Hbn. with T. evanescens Westw. have been reported from Germany, France, Switzerland, West European countries and to control Asian corn borer, O. furnacalis Guenee from the Philippines (Tran and Hassan 1986). Successful attempts to control the grape moth Lobesia botrana in vineyards in Egypt have been reported by El-Wakeil et al. (2008). Hegazi et al. (2004) reported that the combined effect of inundative releases of egg wasps, T. evanscens, with mating disruption technique was successful and could provide a model for control of lepidopterous pests of olive trees.
The use of commercial strain of T. evanescens (TE) as biological control agent for suppression of the olive moth populations is being evaluated in Egypt.
Materials and methods
Field trials
Field experiments were conducted for three successive years (2002–2004) in a commercial olive farm located in the arid olive grove area between Alexandria and Cairo, 177 km south of Alexandria. The farm ‘Paradise Park’ is divided into 88 isolated plots (each 2.3–3.5 ha). Olive trees were planted in early 1996 at a density of 336 trees/ha. Trees were approximately 3–4 m height, planted at 5-m distance along the row and 6-m distance between rows. No applications of Trichogramma releases were previously performed on the farm.
The flight phenology of the OM was monitored by sex pheromones supplied by Prof. B.E. Mazomenos (Chemical Ecology and Natural Products Laboratory, NCSR ‘Demokritos’, Greece). Delta-wing traps (2/ha), baited with polyethylene vials loaded with 1.0 mg Z7-14: Ald, were used for OM. Fresh dispensers were used at the end of each generation. All traps were controlled weekly.
Two olive plots, each 1–1.5 ha, cultivated with the same olive varieties (Shamy and Toffahi) were selected to evaluate the efficacy of the commercially available species (TE). In the first plot, four patches, each contains 4 × 4 trees, were selected for Trichogramma releases (TR). The distance between patches within or between the second plot is <50 m. Patches of the second olive plot were used as control, i.e., without wasp releases (CO). Mass production of TE wasps was carried out by the International Company for Bioagriculture (ICB), Egypt. At each release, a dose of 3,000 wasps/card × 3 cards/tree was applied. In each card, Trichogramma of three different ages were released to keep searching adults present continuously. Eight-eleven releases were performed per year at 2-week intervals from 1 March to the end of October to cover the first (anthophagous), the second (carpophagous) generations and the whole seasonal presence of the jasmine moth, Palpita unionalis (Herz et al. 2005) of the olive moth (OM).
The efficacy of inundative releases of the TE wasps was assessed by comparing egg parasitism and population size of OM (2004-season), pre-mature fruit fall, damage of mature fruits and fruit yield (2002, 2003 and 2004 seasons) in control and Trichogramma-treated trees. Egg parasitism presented on treated trees (TR) and untreated ones (CO) was determined once a week in 2004 growing season. Three sampling points were randomly chosen in the TR and CO sites. On each sampling point, three neighbouring trees were sampled per tree-patch. From each tree, 10 olive shoots (ca 30-cm long) were sampled from each direction for egg counting. In the laboratory, the collected eggs were kept in a climatic chamber (25°C; 70% RH; 16:8 h L:D) until they either hatched or turned black (parasitized) and the emerged wasps were determined to species. When wasps other than TE emerged, fresh Sitotroga cerelella eggs (glued on paper) were added to the sample and labelled with a code name. After parasitization, fresh parasitized egg cards were sent to the Institute for Biological Control (Dr. Annette Herz), Darmstad, Germany, for identification. Identification of local species was carried out by using a diagnosis system combining morphological characters (Pinto 1999) with molecular biological attributes (Silva et al. 1999). The percentages of parasitism by TE/sample were recorded.
Premature fruit drop caused, in part, by OM larvae was recorded by spreading two plastic nets covering the area of projection of two tree canopies per treated tree-patch from time of fruit setting to the harvest. All fallen fruits per plastic sheets were collected and weighed. For each sample, the number of fruits in 100 g/tree was counted to extrapolate the total number of fallen fruits per tree. At each sampling time, 200 fruits per sample were examined to record reasons of dropping.
Fruit damage was assessed in late August to mid-September. Five to six hundred fruits were randomly picked per tree-patch. The mean percentage of fruits with pest damage was computed. OM ‘damage’ for ripe fruits was calculated from characterized mines on fruit surface. At harvest time, fruit yield was assessed by selecting 5–6 trees per tree-patch and the total weight of fruit harvest per tree was determined.
Data analysis
Data were analysed for normal distribution and mean values were compared by Student’s t test. Percentages of data were transformed to arcsin square root of proportions before statistical analysis, but the untransformed mean + SD were presented for comparison (SAS Institute 1989).
Results
Monitoring of the OM was performed for the three successive olive seasons. Only, the results of the third year are shown in Fig. 1, as a representative data for all other years. Based on the number of captured OM males, the first adults appeared as representative for all other years on 24 March when the inflorescence reached stage ‘D’, i.e, before bloom (Arambourg and Pralavorio 1986). Peak catches of the anthophagous generation reached 53.8 + 36.1 moths/week/trap on 21 April coincided with beginning of flower opening, the tree phonological stage ‘F’. Then the trap catches progressively increased and the peak of the fruit generation (carpophagous) reached 126.7 + 46.7 moths/week/trap on 5 May (tree phonological stage ‘G’, i.e., petal fall and fruit setting). No trap catches of the third (phyllophagous) generation were recorded from 4 August to mid-September.
The impact of 8, 11 and 11 TE-wasps releases in 2002, 2003 and 2004, respectively, on the fruit drop of olive trees is shown in Table 1. In high fruiting years (2002 and 2004 olive-seasons), significant (P < 0.05) higher fruit drop occurred on control tree-patches compared with those observed on wasp-treated tree-patches (for total fruit weight: t = 3.14 for 2002; t = 3.7 for 2004, df = 8 at P = 0.05, Fig. 2; for total fruit number: t = 13.6 for 2002, t = 22.5 for 2004, df = 8 at P = 0.05, Fig. 3). However, reverse results were recorded in low fruiting year (2003 olive season, t = 4.9 for total weight, t = 13.6 for total number, df = 8, P = 0.05) (Figs. 2 and 3, respectively).
Damage due to mining of OM larvae, mid to late in the season, throughout three years are shown in Table 1. In all study years, fruit damage was significantly higher (t = 3.9 for 2002, t = 7.9 for 2003, t = 7.3 for 2004, df = 8 at P = 0.05) on tree-patches received no TE wasps compared with those treated with the wasps.
In 2004-olive season, parasitization rates by TE wasps on treated tree-patches were compared with natural parasitization in non-releases tree-patches (Fig. 2). In control tree-patches, the natural parasitism was very low during the flight period (March–June) of OM. The maximum percentage of parasitism reached 13.68% late in 14 July. Trichogramma wasps emerged from naturally parasitized eggs showed that 60.8 and 38.0% were T. cordubensis Vargas and Cabello (TC) and T. euproctidis Girault (TEU), respectively. On the other hand, olive tree-patches received TE releases showed different ranges of parasitism. Parasitism levels ranged from 0.2% on 7 April to 43.5% on 14 July in OM eggs. Emerged wasps from parasitized eggs in the TE tree-patches showed that 21 and 8% of the individuals were TC and TEU, respectively. The TC (thelytokous species) and TEU (arrhenotokous species) were collected several times during the olive seasons.
The effect of inundative releases of TE wasps on the population size of OM larvae was also assessed by recording the full season counts of host larvae/sample/tree on both non-treated and treated tree-patches. Releases of TE wasps caused significant (t = 6.03, df = 8, P = 0.05) total seasonal reduction of 22.5% in OM population (Fig. 3). As far as fruit harvest/tree was concerned, tree-patches received TE-wasps in only 2002 (t = 3.3, df = 8, P = 0.05) and 2004 (t = 5.2, df = 8, P = 0.05) olive seasons yielded significantly greater fruits by 10.5 and 12.5% in 2002 and 2004 than the control trees (Table 1), respectively.
Discussion
The followings were detected: (1) the anthophagous generation closely overlapped with the carpophagous one, (2) the OM density was significantly greater in the high fruiting years (2002 and 2004) than in the low fruiting year (2003, data not shown), (3) monitoring with pheromone trap was not only a good method to detect the presence of adults and to monitor the fluctuation of adult population densities, but also gave useful data for evolution of egg-laying activity on flower and fruits, i.e., a good method for the determination of the most suitable time for Trichogramma releases and (4) besides, both of pre-mature fruit fall caused by feeding damage of the OM during May–August and natural thinning, the OM larvae, mid to late in the season, attacked the mature olive fruits especially table varieties. Damaged fruits deprecated the value of table varieties and lower the quality of oils produced from oil varieties.
The first serious attempts to use Trichogramma on olive trees were undertaken by Stavraki (1977, 1985) in Greece, releasing the species; T. cacoeciae, T. dendrolimi, T. minutum, T. pretiosum, T. euproctidis and two unidentified strains against the carpophagous generation of the olive moth in several field trials. These species/strains had not been collected from olive groves, but some of them were successfully used in other crop systems. TE wasps are known to attack a wide variety of field crop moths (Abbas et al. 1987; Ram et al. 1995; Bayram and Kornosor 1999; Herz et al. 2005). TE parasitoid was extensively used in inundative releases against a number of lepidopterous pests of several crops (e.g., corn, rice, sugarcane, cotton, fruit trees, grape, etc.) (Ram et al. 1995; El-Wakeil et al. 2008), but had not been observed in olive groves. Releases of TE wasps were carried out in an olive farm that represent a young and large intensively managed plantations under arid weather condition and TE free. The obtained results were encouraging since OM attacks were reduced and TE-treated trees yielded significantly greater fruits than the control trees.
Releases were made at a rate of 9,000 wasps/tree (3,000,000 wasps/ha). This dose was fairly larger to what has been used in other crop system. Rate of the parasitoid releases adopted in other several studies ranged from 15,000 (Chen and Chiu 1986) to 450,000 adults/ha (Cock 1985). However, field parasitization levels by TE indicated poor adaptation of TE to local environmental conditions of olive groves. Despite the widespread use of Trichogramma, there are relatively few cases where the successful control of a pest can be unequivocally ascribed to releases of these parasitoids. There are many documented failures of Trichogramma releases despite a few notable successes (Twine and Lloyd 1982; Smith et al. 1987; Li 1994). Sithanantham et al. (2001) reported that Trichogrammatid parasitoids are more habitat-specific than host-specific. They also reported that when selecting the species to be used, the naturally occurring interspecific diversity and the specialization should be considered. A local species is generally preferred on the basis that it is likely to be better adapted to the ecological conditions than an exotic species (Smith 1996). The obtained results indicated that pest control by naturally occurring Trichogramma egg parasitoids was insufficient and local augmentative releases of reared wasps are needed. In olive groves, however, higher levels of egg parasitism were obtained by local strains of TC wasps in OM eggs (up to 59%) in Portugal and in jasmine moth eggs, P. unionalis (83%, in Egypt, Herz et al. 2005). Strains described in this study could all be easily propagated on factitious hosts (S. cereallela, E. kuehniella) (e.g., Ayvaz and Karaborklu 2008; El-Wakeil 2007), thus indicating their potential for mass production, one important prerequisite for their use as biological control agents. Thus, one of the suggested measures to improve the quality of the parasitoid in release programme is to release the endemic wasp species in olive groves, e.g., TC or TEU or both together. Hegazi et al. (2007) suggested that releases of the indigenous TC and TEU could improve control of lepidopterous pests on olive. The present study points on the need for monitoring the local Trichogramma species in a particular area before inundative releases of species are conducted for the first time.
References
Abbas MST, El-heneidy AH, El-sherif SI, Embaby MM (1987) On utilization of Trichogramma evanescens Westw. to control the lesser sugarcane borer, Chilo agamemnon Bles. In sugarcane fields in Egypt. I. Pilot Studies. Bull Soc Ent Egypt Eco Ser 17:47–62
Arambourg Y, Pralavorio R (1986) Hyponomeutidae. Prays oleae Bern. In: Arambourg, Y (ed) Traite d’entomologie oleicole. International Olive Oil Council, Madrid, Spain, pp 47–91
Ayvaz A, Karaborklu S (2008) Effect of cold storage and different diets on Ephestia kuehniella Zeller (Lep: Pyralidae). J Pest Sci 81:57–62
Bayram A, Kornosor S, (1999) Biological features of Trichogramma evanescens Westwood (Hymenoptera; Trichogrammatidae) on the eggs of Sesamia nonagrioides Lefevbre (Lepidoptera; Noctuidae). Proceedings of the XX Conference of the International Working Group on Ostrinia and other Maize Pests, 4–10 Sept, Adana, Turkey, pp 165–170
Chen CC, Chiu SC (1986) Studies on the field releases of Trichogramma chilonis and factors affecting its activity. J Agric Res China 35:99–106
Cock MJW (1985) The use of parasitoids for augmentative biological control of pests in the Peoples Republic of China. Biocontr News Inf 6:213–223
El-Wakeil NE (2007) Evaluation of the efficiency of Trichogramm evanescens on different factitious hosts to control Helicoverpa armigera. J Pest Sci 80:109–117
El-Wakeil NE, Farghaly HT, Ragab ZA (2008) Efficacy of inundative releases of Trichogramma evanescens in controlling Lobesia botrana in vineyards in Egypt. J Pest Sci 81:49–55
Hassan SA, Hafez B, Degrande PE, Herai K (1998) The side effects of pesticides on the egg parasitoid Trichogramma caecoeciae Marchal (Hym.; Trichogrammatidae), acute dose-response and persistence tests. J Appl Entomol 122:569–573
Hegazi EM, Agamy E, Hassan S, Hertz A, Khafagi W, Showell S, Abo abd-allah L, Ziton A, Hafez M, El-shazly A, El-said S, El-minshawy A, Kram H, Khamiss N, El-kemny S (2004) Preliminary study on the combined effect of mating disruption and inundative releases of Trichogramma evanescens against the olive moth, Prays oleae. Egypt J Biol Pest Control 14:9–14
Hegazi EM, Herz A, Hassan SA, Khafagi WE, Agamy E, Zaitun A, El-said S, Abd el-aziz G, Khamiss N (2007) Field efficiency of indigenous egg parasitoids (Hymenoptera : Trichogrammatidae) to control the olive moth (Prays oleae, Lepidoptera; Yponomeutidae) and the jasmine moth (Palpita unionalis, Lepidoptera; Pyralidae) in an olive plantation in Egypt. Biol Control 43:171–187
Herz A, Hassan SA, Hegazi E, Khafagi WE, Nasr FN, Youssef AA, Agamy E, Jardak T, Ksantini M, Mazomenos BE, Konstantopoulou MA, Torres L, Goncalves F, Bento A, Pereira JA (2005) Towards sustainable control of Lepidopterous pests in olive cultivation. Gesunde Pflanzen 58:117–128
Honda JY (2005) Partitioning native and augmentative Trichogramma platneri (Hymenoptera; Trichogrammatidae) parasitism of Amorbia cuneana (Lepidoptera; Tortricidae) egg mass in southern California avocado orchards. Florida Entomol 88:325–326
Li LY (1994) Worldwide use of Trichogramma for biological control on different crops. A survey. In: Wajnberg E, Hassan SA (eds) Biological control with egg parasitoids. CAB International, Oxon, pp 37–51
Lopes-Vellata MC (1999) Olive pest and disease management. International Olive Oil Council, Principe de Vergara, 154-28002, Madrid, p 206
Mazomenos DE, Ortz A, Mazomenos-pantazi A, Stefanou D, Stavrakis N, Karapati C, Fountoulakis M (1999) Mating disruption for the control of the olive moth. Prays oleae (Bern.) (Lep.: Yponomeutidae) with the major sex pheromone component. J Appl Ent 123:247–254
Mills N, Pickel C, Mansfield S, Mcdougall S, Buchner R, Caprile J, Edstrom J, Elkins R, Hasey J, Kelley K, Krueger B, Olson B, Stocker R (2000) Mass releases of Trichogramma wasps can reduce damage from codling moth. Calif Agric 54:22–25
Öztemiz S, Kornosor S (1999) Biological control of Ostrinia nubilalis Hubner (Lepidoptera; Pyralidae) by Trichogramma evanescens Westwood (Hymenoptera; Trichogrammatidae) and its natural parasitization rate on maize in Cukurova region of Turkey. Proceedings of the XX Conference of the International Working Group on Ostrinia and other Maize Pests, 4–10 Sept, Adana, Turkey, pp 122–130
Patanita MI, Mexia A (2004) Loss assessment due to Prays oleae Bern. and Bactrocera oleae Gmelin in Moura’s region (Portugal) (WWW document). http://pubol.ipbeja.pt/Artigos/Italia.htm
Pelekasis CE (1962) A contribution to the study of nomenclature, taxonomy, biology and natural parasitization of the olive kernel borer Prays oleae (Bern). Annales de l’Institut Phytopathologique, Benaki (NS), 4, 180–308
Pinto JD (1999) Systematics of the North American species of Trichogramma Westwood (Hymenoptera; Trichogrammatidae). Mem Entomol Soc, Washington 22:1–287
Pintureau B (1990) Polymorphisme, biogeography et specificite parasitaire des Trichogrammes europeens (Hym.: Trichogrammatidae). Bull Soc Entomol Fr 95:17–38
Ram P, Tshernyshev WP, Afonina VM, Greenberg SHM (1995) Studies on the strains of Trichohgramma evanescens Westwood (Hym.: Trichogrammatidae) collected from different hosts in Northern Maldova. J Appl Ent 199:79–82
Ramos P, Campos M, Ramos JM (1998) Long-term study on the evaluation of yield and economic losses caused by Prays oleae Bern. in the olive crop of Granada (southern Spain). Crop Prot 17:645–647
SAS Institute INC (1989) SAS/STAT® User’s Guide, Version 6, vol 1, 4th edn. SAS Institute Inc, Cary, NC, p 943
Silva IMMS, Honda J, Vankan F, Hu J, Neto L, Pintureau B, Stouthammer R (1999) Molecular differentiation of five Trichogramma species occurring in Portugal. Biol Control 16:177–184
Sithanantham S, Abera TH, Baumgartener Hassan SA, Lohr B, Monje JC, Overholt WA, Paul AVN, Fanghao WAN, Zebitz CPW (2001) Egg parasitoids for augmentative biological control of lepidopteran vegetable pests in Africa. Insect Sci Appl 21:189–205
Smith SM (1996) Biological control with Trichogramma: advances, successes and potential of their use. Annu Rev Entomol 41:375–406
Smith SM, Hubbes M, Carrow JR (1987) Ground releases of Trichogramma minutum Riley (Hymenoptera; Trichogrammatidae) against the spruce budworm (Lepidoptera; Tortricidae). Can Entomol 119:251–263
Stavraki HG (1977) Results obtained from releases of the oophagous parasites Trichogramma sp. against Prays oleae Bern. (Lep.: Hyponomeutidae) over a four year period in Greece. Med Fac Landbouww Rijksuniv Gent 42:1361–1371
Stavraki HG (1985) Use of Trichogramma spp. against the carpophagous generation of Prays oleae (Bern.) in Greece. In: Cavalloro R, Crovetti A (eds). Proceedings of the CEC/FAO/OBC International Joint Meeting, Pisa, Italy, 3–6 April, 1984, Balkema, Rotterdam, pp 242–246
Tran LC, Hassan SA (1986) Preliminary results on the utilization of Trichogramma evanescens West. to control the Asian corn borer. J Appl Entomol 101(11):18–23
Twine PH, Lloyd RJ (1982) Observations on the effect of regular releases of Trichogramma spp. in controlling Heliothis spp. and other insects in cotton. Qld J Agri Anim Sci 39:159–167
Ulrichs Ch, Mewis I (2004) Evaluation of the efficacy of Trichogramma evanescens Westwood (Hym.; Trichogrammatidae) inundative releases for the control of Maruca vitrata F. (Lep.; Pyralidae). J Appl Entomol 128:431–624
Usun S, Kavut H, Goven M A, Kartal S (1996) Studies on releasing of Trichogramma brassicae Bezd. (Hym.; Trichogrammatidae) for biological control of Ostrinia nubilalis Hbn. (Lep.; Pyralidae) which is harmful in maize fields in Aydin. Proceedings of the Third National Congress of Entomology, 24–28 Sept, Ankara, pp 320–327
Wright MG, Hoffmann MP, Chenus SA, Gardner J (2001) Dispersal behavior of Trichogramma ostriniae (Hymenoptera; Trichogrammatidae) in sweet corn fields: Implications for augmentative releases against Ostrinia nubilalis (Lepidoptera; Crambidae). Biol Control 22:29–37
Yamvrias C, Broumas T, Liaropoulos C, Anaghou M (1986) Lutte contre la teigne de l’olivier avec une preparation biologigue. Anales de l’Institut Phytopathologigue Benaki (NS) 15:1–10
Acknowledgements
This work was conducted with financial support from the European Commission within the specific programme ‘Confirming the International Role of Community Research’, contract ICA4-CT-2001-1004 (TRIPHELIO: Sustainable control of lepidopterous pests in olive groves—integration of egg-parasitoid and pheromones). The author would like to thank Dr. Annette Herz, Institute for Biological Control, Darmstadt, Germany, for identifying the local parasitoids and for her technical advice.
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Agamy, E. Field evaluation of the egg parasitoid, Trichogramma evanescens West. against the olive moth Prays oleae (Bern.) in Egypt. J Pest Sci 83, 53–58 (2010). https://doi.org/10.1007/s10340-009-0273-x
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DOI: https://doi.org/10.1007/s10340-009-0273-x