Abstract
Accumulating evidence suggests that cognitive declines in old (healthy) animals could arise from depression of intracortical inhibition, for which a decreased ability to produce GABA during senescence might be responsible. By simulating a neural network model of a primary visual cortical (V1) area, we investigated whether and how a lack of GABA affects cognitive performance of the network: detection of the orientation of a visual bar-stimulus. The network was composed of pyramidal (P) cells and GABAergic interneurons such as small (S) and large (L) basket cells. Intrasynaptic GABA-release from presynaptic S or L cells contributed to reducing ongoing-spontaneous (background) neuronal activity in a different manner. Namely, the former exerted feedback (S-to-P) inhibition and reduced the frequency (firing rate) of action potentials evoked in P cells. The latter reduced the number of saliently firing P cells through lateral (L-to-P) inhibition. Non-vesicular GABA-release, presumably from glia and/or neurons, into the extracellular space reduced the both, activating extrasynaptic GABAa receptors and providing P cells with tonic inhibitory currents. By this combinatorial, spatiotemporal inhibitory mechanism, the background activity as noise was significantly reduced, compared to the stimulus-evoked activity as signal, thereby improving signal-to-noise (S/N) ratio. Interestingly, GABA-spillover from the intrasynaptic cleft into the extracellular space was effective for improving orientation selectivity (orientation bias), especially when distractors interfered with detecting the bar-stimulus. These simulation results may provide some insight into how the depression of intracortical inhibition due to a reduction in GABA content in the brain leads to age-related cognitive decline.
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Introduction
It is well known that aging adversely affects cognitive brain functions in humans. Namely, cognitive performance advances progressively from infancy to young adulthood and then declines toward old age. Salthouse (1996) provided clear evidence of age-related cognitive decline, in which the processing speed of perceptual tasks (e.g., letter comparison and pattern comparison) increased from infancy to young adulthood and then decreased toward old age. Deficit in memory recall is another common problem in aging (Cohen and Burke 1993). Old (healthy) subjects had difficulty in accessing stored information, or memories, even though there had been no decrease in memory performance in that they were able to retrieve information with better cues. Craik and Bialystok (2006) speculated that poor cognitive performance in infancy might be due to incomplete representation of information and that in old age might be associated with difficulties in accessing the information.
Although little is known about the underlying neuronal mechanism of age-related cognitive decline, experimental studies provided some insight into it. Schmolesky et al. (2000) demonstrated that old macaque monkeys exhibited decreased orientation selectivity and direction selectivity accompanied by a greater increase in ongoing-spontaneous activity than in stimulus-evoked activity in V1 (primary visual cortex) cells, resulting in a decrease in signal-to-noise (S/N) ratio. Their follow-up study (Leventhal et al. 2003) investigated how gamma-aminobutyric acid (GABA) affects responses of V1 cells to oriented bars presented to visual receptive fields. Administration of GABA enhanced S/N ratio, accompanied by a greater decrease in ongoing-spontaneous activity than in stimulus-evoked activity in V1 cells. The researchers suggested that age-related cognitive decline might arise from depression of intracortical inhibition, for which a decreased ability to produce GABA during senescence might be responsible. Important questions still unanswered are as follows: how is the depression of intracortical inhibition caused, and how does it lead to age-related cognitive decline?
Distinct modes of GABA-release into target regions might be responsible for intracortical inhibition, for example, (1) intrasynaptic release into the synaptic cleft triggered by a presynaptic action potential, (2) its spillover into the extracellular space, and iii) non-vesicular release, presumably from glia and/or neurons, into the extracellular space (Semyanov et al. 2004). The first type of GABA-release activates intrasynaptic GABAa receptors, while the second and third ones activate extrasynaptic GABAa receptors (Somogyi et al. 1989; Nusser et al. 1995; Brickley et al. 1996; Soltesz and Nusser 2001). Extrasynaptic GABAa receptors have been found in the cerebellum and cortex (Drasbek and Jensen 2006; Scimemi et al. 2006).
In the cortex, anatomical and physiological properties of GABAergic interneurons are markedly diverse (Gupta et al. 2000). Typical GABAergic interneurons found in the cortex include basket cells, bitufted cells, and martinotti cells. It is also well known that about 50 % of GABAergic cortical interneurons are basket cells (Markram et al. 2004). Basket cells tend to synapse on pyramidal cells (Somogyi et al. 1983), have diversity especially in their axonal arborizations, and are classified roughly into two subclasses: large and small basket cells (Wang et al. 2002). The large basket cells and small basket cells may correspond to wide arbor basket cells and local arbor basket cells, respectively (Krimer and Goldman-Rakic 2001), which have aspiny wide (up to ∼1000 μm) and narrow (up to ∼300 μm) axonal arbors, and synapse mostly on the somata of target cells.
In the primary visual cortex, a long range inhibitory system made of large basket cells provides broadband inhibition toward its target cells (Kisvarday et al. 1993). A single large basket cell provides input to regions representing the whole range of orientations, that is, to the iso-orientation (±30°), oblique-orientation (±[30 − 60]°), and cross-orientation (±[60 − 90]°) columns (Kisvarday and Eysel 1993; Buzas et al. 2001). This implies that the large basket cells might have a lateral inhibitory effect on pyramidal cells via their long and wide axonal arbors and contribute to tuning to the orientations (angles) of bar-stimuli.
It is also well known that excitatory and inhibitory neurons frequently form reciprocal synaptic connections (Martin 2002). Zilberter (2000) has reported that 75 % (n = 80) of pairs of a pyramidal cell and an interneuron form reciprocal synaptic connections. The interneuron might be small basket cell or chandelier cell (Krimer and Goldman-Rakic 2001). Such reciprocal connections are likely to mediate feedback inhibition as the activity of pyramidal cells becomes greater.
Based on these experimental observations, we construct a neural network model of an orientation preference map, which is simple and functional but involves key neuronal elements. Orientation columns consist of cell units. A cell unit contains one pyramidal cell, one small basket cell, and one large basket cell. In the model, we assume three distinct modes of GABA-release into target regions as addressed previously, namely intrasynaptic release into the synaptic cleft, its spillover into the extracellular space, and non-vesicular release into the extracellular space. We investigate how these modes of GABA-supply affect the performance of the V1 network (signal-to-noise ratio, orientation selectivity, and reaction time), and how the depression of intracortical inhibition is caused and how it leads to age-related cognitive decline.
Methods
A functional, minimal neural network model of an orientation preference map is schematically illustrated in Fig. 1a. Each domain (θ n : 0 ≤ n ≤ 7), so-called orientation column, has a preference to one particular orientation, ranging from 0 to 7π/8 (radian), and consists of cell units as shown in Fig. 1b (“gray oval”). A cell unit contains one pyramidal cell (“P”), one small basket cell (“S”), and one large basket cell (“L”). Within the same column, P cells receive excitatory projections from each other. Each P cell receives an inhibitory projection from its accompanying S cell and receives inhibitory projections from L cells that belong to other orientation columns. An excitatory current (I LGN(θ)) is provided as a sensory input to the corresponding P cells when presented with a sensory stimulus (θinp). The S and L cells have narrow and wide axonal arbors and provide feedback and lateral inhibitory effects on P cells, respectively. Details of the network dynamics regulated by these interneurons have been reported in our previous studies (Hoshino 2006, 2008a; Totoki et al. 2010; Fujiwara et al. 2011; Miyamoto et al. 2012). For model descriptions, see Appendix.
Results
Neuronal responses to visual stimulation
In this section, we show how neurons respond to visual stimulation. Intra- and extrasynaptic (ambient) GABA concentrations are varied, mimicking young and old (healthy) subjects, in order to see whether and how they affect a simple perceptual task: detection of a visual bar-stimulus.
Figure 2a presents membrane potentials (top) and raster plots of action potentials (bottom) evoked in pyramidal (P) cells of the network assumed for a young subject, where an oriented (θ = 5π/8) bar-stimulus was presented briefly (see the horizontal bar). Figure 2b presents those assumed for an old subject in which the amounts of intrasynaptic and non-vesicular GABA-release were reduced to half: [GABA] K j (θ; t) = GABA K = 0.5 mM (K = S, L) and [GABA]0 = 0.5 μM (see Eqs. 12, 14 in Appendix). In the old network, both the ongoing-spontaneous (background) and stimulus-evoked neuronal activities are increased.
Figure 3a presents the time course of ambient GABA concentration in the young (thick trace) or old (thin trace) network. The overall GABA concentration in the old network is lower than that in the young network. The transient increase in ambient GABA concentration, triggered by the stimulus, arises from GABA-spillover (from synaptic clefts) into the extracellular space. Figure 3b (left) presents firing rates (“circles”) of a P cell, when presented with a series of orientations (0 ≤ θ ≤ 7π/8). S/N ratio (“squares”) is the ratio of the stimulus-evoked firing rate to the ongoing-spontaneous firing rate for the young (top-left) or old (bottom-left) network. These results indicate that the P cell has its orientation preference to θ = 5π/8.
To quantitatively evaluate the orientation preference, we calculated orientation bias (OB), which is frequently used for visual systems such as the lateral geniculate nucleus (LGN) (Xu et al. 2002) and the primary visual cortex (Leventhal et al. 1995). We briefly explain it. Responses of a cell to different orientations (0, π/8, 2π/8, 3π/8, 4π/8, 5π/8, 6π/8, 7π/8) are stored as a series of vectors. The vectors are added and divided by the sum of the absolute values of the vectors. The angle and the length of the resultant vector provide, respectively, the preferred direction and the degree of orientation preference of the cell. The degree of orientation preference is termed “orientation bias (OB).” Note that since the periodicity of orientation is π, the angles of bar-stimuli are multiplied by a factor of two. As a result, OB ranges from 0 to 1.0, with 0 being completely insensitive to orientation and 1.0 responding to only one orientation. As shown in Fig. 3b, OB = 0.712 for the young network (top-right) and 0.583 for the old network (bottom-right) indicate that the orientation preference is deteriorated in the old network. Experimental studies (Schmolesky et al. 2000; Leventhal et al. 2003) reported a similar tendency.
Next, we investigated how intrasynaptic GABA molecules supplied by small (S) and large (L) basket cells ([GABA] S j (θ; t) = GABA S and [GABA] L j (θ; t) = GABA L ; see Eq. 12 in Appendix) and ambient (extrasynaptic) GABA molecules supplied by non-vesicular release ([GABA]0; see Eq. 14 in Appendix) affect the network performance. Figure 4a (left) presents firing rates of a P cell during the ongoing-spontaneous (“triangles”) and stimulus-presentation (“circles”) time periods, S/N ratio (“squares”), and OB (right) as a function of GABA S . Figure 4b, c are those for GABA L and [GABA]0, respectively. We found that these (intrasynaptic and non-vesicular) GABA-release modes can improve S/N ratio, when neuronal suppression is greater in ongoing-spontaneous (background) activity than in stimulus-evoked activity. Note that the L cells contribute to responding selectively to the applied stimulus, that is, to improving OB (Fig. 4b; right).
Influences of intrasynaptic and non-vesicular GABA-release on ongoing-spontaneous neuronal activity
As has been shown in the previous section, the reduction in ongoing-spontaneous neuronal activity is essential for improving S/N ratio. In this section, we show how the intrasynaptic and non-vesicular GABA-release modes affect the ongoing-spontaneous neuronal activity.
Figure 5a presents the dependence of ongoing-spontaneous neuronal activity on intrasynaptic (S-to-P) GABA concentration. A reduction in intrasynaptic GABA concentration (bottom; GABA S = 0.1 mM) results in greater action potential generation. A notable finding is that the lower GABA concentration makes P cells generate action potentials in a dense manner in “time,” emitting longer bursts, which are quantitatively shown in Fig. 5b (right). The longer bursts arise from depressed feedback inhibition due to a decrease in intrasynaptic GABA-release from presynaptic S cells.
Figure 6 presents the dependence of ongoing-spontaneous neuronal activity on intrasynaptic (L-to-P) GABA concentration. A reduction in intrasynaptic GABA concentration (bottom; GABA L = 0.1 mM) results in greater action potential generation. A notable finding is that the lower GABA concentration makes P cells generate action potentials in a dense manner in “space,” where multiple dynamic cell assemblies are allowed to emerge at the same time. This overlapping neuronal behavior arises from depressed lateral inhibition due to a decrease in intrasynaptic GABA-release from presynaptic L cells.
Figure 7a presents the dependence of ongoing-spontaneous neuronal activity on basal concentration that is supplied by non-vesicular GABA-release: [GABA]0. Its reduction (bottom; [GABA]0 = 0.1 μM) results in greater action potential generation, in which P cells generate action potentials in a dense manner in both “time” and “space.” The lower ambient GABA concentration increases the overall neuronal activity (firing rate) as shown in Fig. 7b (see the solid trace).
These results indicate that the intrasynaptic and non-vesicular GABA-release modes contribute to reducing ongoing-spontaneous (background) neuronal activity in different manners. Namely, the intrasynaptic feedback inhibition by presynaptic S cells reduces the frequency (firing rate) of action potentials, the intrasynaptic lateral inhibition by presynaptic L cells reduces the number of saliently firing neurons, and the non-vesicular GABA-release mode reduces the both. By this combinatorial, spatiotemporal inhibitory mechanism, the background activity as noise can be significantly reduced, compared to the stimulus-evoked activity as signal, thereby improving the S/N ratio.
Significance of GABA-spillover for sensory information processing
In this section, we show whether and how GABA-spillover from synaptic clefts into the extracellular space affects the network performance, if the perception of a bar is interfered with distractors.
Figure 8a presents the dependence of orientation bias (OB) on the ratio of stimulus (θ2) to distractor (θ n : n ≠ 2) intensity, indicating that GABA-spillover works for OB enhancement (“change in OB”), when the fraction of distractors increases (“circles”). As shown in Fig. 8b, the distractors accelerate the intrasynaptic GABA-release from presynaptic S and L cells and thus enhance GABA-spillover, thereby increasing the ambient GABA concentration (see the upper arrows).
As shown in Fig. 9a, we compared reaction time of P cells to the stimulus between these two cases: with (top) or without (middle) GABA-spillover. Profiles of ambient GABA concentrations are shown at the bottom. Note that the maximal ambient GABA concentrations were adjusted to the same value (bottom), by which we could obtain almost identical OB values for these two cases. As shown in Fig. 9a (middle) and will be quantitatively shown in Fig. 10a (right), the ongoing-spontaneous membrane potentials of P cells tend to hyperpolarize, if the GABA-spillover does not take place. This membrane hyperpolarization results in prolonging the reaction time to the stimulus as shown in Fig. 9b (bottom). Figure 10a presents distributions of ongoing-spontaneous membrane potentials of a P cell with (left) and without (right) GABA-spillover, respectively, indicating that the GABA-spillover mechanism allows the P cell to depolarize below firing threshold (left).
As a ready state for sensory input, the ongoing-spontaneous activity has a great impact on subsequent neuronal information processing (Hoshino 2008b, 2009, 2010, 2011a). Figure 10b shows a relationship between the reaction time of a P cell and its ongoing-spontaneous membrane potential just (1 msec) before the stimulus onset. The same stimulus was presented repeatedly and arbitrary in time. We found that the greater the membrane depolarization, the shorter the reaction time. This result indicates that the ongoing-spontaneous subthreshold neuronal state, achieved by the GABA-spillover mechanism, allows the network to respond rapidly to subsequent sensory stimulation.
Discussion
Accumulating evidence suggests that age-related cognitive decline could arise from depression of intracortical inhibition, for which a decreased ability to produce GABA during senescence might be responsible. By simulating a neural network model of a primary visual cortical (V1) area, we investigated whether and how a lack of GABA affects perceptual performance of the network: detection of the orientation of a visual bar-stimulus. The network was composed of pyramidal (P) cells, small basket (S) cells, and large basket (L) cells. To exert intracortical inhibition, the network was supplied with GABA in three distinct manners: (1) intrasynaptic release into synaptic clefts, (2) non-vesicular release, presumably from glia and/or neurons, into the extracellular space, and iii) spillover from synaptic clefts into the extracellular space. By simulating the network model, we investigated how GABA affects signal-to-noise (S/N) ratio, orientation bias (OB), and reaction time.
Intrasynaptic GABA-release from presynaptic S or L cells contributed to reducing ongoing-spontaneous (background) neuronal activity in a different manner. Namely, the former exerted feedback (S-to-P) inhibition and reduced the frequency (firing rate) of action potentials evoked in P cells. The latter reduced the number of saliently firing P cells through lateral (L-to-P) inhibition. Non-vesicular GABA-release into the extracellular space reduced the both, activating extrasynaptic GABAa receptors and providing P cells with tonic inhibitory currents. By this combinatorial, spatiotemporal inhibitory mechanism, the background activity as noise was significantly reduced, compared to the stimulus-evoked activity as signal, thereby improving the signal-to-noise (S/N) ratio. Interestingly, GABA-spillover from the synaptic cleft into the extracellular space was effective for improving the stimulus selectivity (orientation bias), especially when distractors interfered with detecting the bar-stimulus. These simulation results may provide some insight into how the depression of intracortical inhibition due to a reduction in GABA content in the brain leads to age-related cognitive decline.
In general, if neuronal activity, for any reason, happens to be increased (e.g., by noise from external environments or by irrelevant bombardment from other brain areas), ongoing-spontaneous neuronal activity would be increased and thus S/N ratio worsened. This problem can be overcome, provided that the GABA-spillover mechanism works. Namely, the greater the ongoing-spontaneous activity is, the greater the GABA spills over into the extracellular space, and therefore the greater the suppression of ongoing-spontaneous neuronal activity. This "self-inhibitory” system, mediated by the neuronal activity-dependent GABA-spillover mechanism, may contribute to maintaining the background neuronal activity at a low level and, therefore, to keeping S/N ratio relatively high.
The neuronal activity-dependent GABA-spillover mechanism was also effective in order to keep P cells oscillating near firing threshold (see Fig. 10), by which the reaction speed to sensory stimulation was accelerated. We suggest that the ongoing-spontaneous subthreshold neuronal state, achieved through GABA-spillover, may work as a ready state preparing for subsequent sensory input.
As discussed in detail by Leventhal et al. (2003), the depression of intracortical inhibition could result from diminished transmitter-release, diminished transmitter-production, and/or degraded transmitter-receptors. The researchers could not point to which is deteriorated in old animals, but they clearly demonstrated that administration of GABA facilitated visual function in old monkeys. Therefore, it was tempting to speculate that a decreased ability to produce GABA in the cortex might be responsible for age-related cognitive decline. The reduction in intrasynaptic and extrasynaptic (ambient) GABA levels, assumed for the old network, was a simple functional representation based on their speculation.
To examine whether and how GABAgergic inhibition contributes to stimulus selectivity and competition among superimposed stimuli (bars with different orientations), Katzner et al. (2011) recorded spiking activity in primary visual cortical (V1) neurons after local iontophoresis of gabazine, a GABAa receptor antagonist. Gabazine broadened the orientation tuning curves of V1 neurons, by raising their responses to all orientations. Gabazine did not affect cross-orientation suppression, the competition seen when stimuli of different orientations are superimposed. They concluded that GABAergic inhibition in V1 enhances stimulus selectivity but is not responsible for competition among superimposed stimuli.
The latter conclusion contradicts our finding: GABAergic inhibition affected the competition among superimposed stimuli, increasing orientation bias (OB: see Fig. 8a). We showed that the GABAergic inhibition arising from GABA-spillover (see Fig. 8b) was responsible for that competition. In our simulation, even without the GABA-spillover, the extrasynaptic GABAa receptor still worked because of the basal ambient GABA concentration (see the trace marked by “without GABA-spillover” in Fig. 8b).
In their experiment (Katzner et al. 2011), gabazine presumably abolishes the whole GABAa receptor-mediated current in which a current arising from GABA-spillover would be involved. Our simulation result may reflect a unique impact of GABA-spillover on the competition between the presented stimulus and distractors, which we hope will be evidenced by future experiments.
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We express our gratitude to the reviewers for giving us valuable comments and suggestions on the earlier draft of this article.
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Appendix
Appendix
The neural network model
Dynamic evolution of the membrane potential of the ith pyramidal (P) cell that belongs to orientation column θ is defined by
where I ex i,rec (θ; t) is a recurrent excitatory postsynaptic current within orientation columns, I ih i,fed (θ; t) a feedback inhibitory postsynaptic current, I ih i,lat (θ; t) a lateral inhibitory postsynaptic current, I ihext (t) an inhibitory non-postsynaptic current caused by GABA-spillover from synaptic clefts and non-vesicular GABA-release into the extracellular space, and I LGN(θ) an excitatory input current triggered by an oriented bar-stimulus (θinp). These currents are defined by
Dynamic evolution of the membrane potentials of small basket (S) cells and large basket (L) cells is defined by
where I S i (θ; t) and I L i (θ; t) are excitatory postsynaptic currents and defined by
In these equations, c Y m is the membrane capacitance of Y (Y = P, S, L) cell, u Y i (θ; t) the membrane potential of the ith Y cell of θ column at time t, g Y m the membrane conductance of Y cell, and u Yrest the resting potential. \(\hat{g}_{Z}\) and u Zrev (Z = AMPA or GABA) are, respectively, the maximal conductance and the reversal potential for the current mediated through Z-type receptor. N θ is the number of cell units constituting each orientation column. w ex ij,rec (θ) and w ih i,fed (θ) are, respectively, the excitatory synaptic strength from the jth to the ith P cell and the inhibitory synaptic strength from S-to-P cell of unit i within columns. w ih ij,lat (θ, θ′) is the inhibitory synaptic strength from the jth L cell of θ′ column to the ith P cell of θ column (θ′ ≠ θ). w S i (θ) and w L i (θ) are, respectively, the excitatory synaptic strengths from P to S cell and to L cell within unit i. θinp in Eq. 6 denotes the orientation (angle) of an input stimulus (a bar).
r P j (θ; t) is the fraction of AMPA-receptors in the open state induced by a presynaptic action potential of the jth P cell belonging to θ column, and r Y j (θ; t) is that of GABAa receptors induced by the jth presynaptic S cell (Y = S) or by the jth presynaptic L cell (Y = L). r GABAext (t) is the fraction of GABAa receptors in the open state, which are located on extrasynaptic membrane regions of P cells. δ denotes a relative amount of extrasynaptic GABAa receptors. Dynamics of these receptors are described by Destexhe et al. (1998)
where α z and β z (z = AMPA or GABA) are positive constants. [T] Y j (θ; t) (T = Glut or GABA) is the concentration of glutamate or GABA in the synaptic cleft. [T] Y j (θ; t) = T Y for 1 ms when the jth presynaptic Y cell fires, and 0 otherwise. [GABA]ext(t) is ambient (extrasynaptic) GABA concentration and defined by
where τext is a time constant for ambient GABA concentration, and [GABA]0 is a basal (resting GABA) concentration determined by non-vesicular GABA-release. The second term (on the right-hand side of Eq. 14) describes a relative amount of GABA-spillover from the synaptic clefts of presynaptic S and L cells. C ext is a positive constant, and τdec determines a degree of contribution of previously released 1 ms pulses of GABA K (K = S, L). We assume that GABA molecules diffuse rapidly across the network.
Probability of firing of the jth Y cell belonging to θ column is defied by
where η Y and \(\zeta_Y\) are, respectively, the steepness and the threshold of the sigmoid function. After firing, the membrane potential is reset to the resting potential.
Unless otherwise stated, c P m = 0.5 nF, c S m = 0.2 nF, c L m = 0.5 nF, g P m = 25 nS, g S m = 20 nS, g L m = 25 nS, u Prest = −65 mV, and u Srest = u Lrest = −70 mV (Koch 1999; McCormick et al. 1985; Kawaguchi and Shindou 1998). \(\hat{g}_{\rm AMPA}\) = 0.5 nS, \(\hat{g}_{\rm GABA}\) = 0.7 nS, u AMPArev = 0 mV, and u GABArev = −80 mV. Each orientation column consists of ten cell units: N θ = 10. w ex ij,rec (θ) = 5.0, w ih i,fed (θ) = w ih ij,lat (θ, θ′) = 1.0, w S i (θ) = w L i (θ) = 10.0. δ = 1000.0, c 0 = 5.0 × 10−10, c 1 = 1.0. αAMPA = 1.1 × 106, αGABA = 5.0 × 105, βAMPA = 190.0, βGABA = 180.0, Glut P = GABA S = GABA L = 1.0 mM, τext = 1.0, [GABA]0 = 1 μM, C ext = 0.2, τdec = 10.0, η P = η S = η L = 350.0, and \(\zeta_{P}\) = \(\zeta_{S}\) = \(\zeta_{L}\) = −50 mV. For these values, see our previous studies (Hoshino 2006, 2008a, b, 2009, 2010, 2011a, b).
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Miyamoto, A., Hasegawa, J. & Hoshino, O. Dynamic modulation of an orientation preference map by GABA responsible for age-related cognitive performance. Cogn Process 13, 349–359 (2012). https://doi.org/10.1007/s10339-012-0524-2
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DOI: https://doi.org/10.1007/s10339-012-0524-2