Summary
Because of an inherent dependence on host cell second and third messenger signaling pathways for activation of HIV-1 expression, a potential exists for a relationship between the induction of latent HIV-1 and cell-cycle-related events. To investigate this potential relationship, cellular models of latent HIV-1 infection (OM-10.1 promyelocytes, ACH-2 T-lymphocytes, and U1 promonocytes) were chemically treated or γ-irradiated to synchronize cultures at each cell cycle stage and then examined for constitutive and TNF-α-induced HIV-1 expression. Cell cycle synchronization alone had no effect on HIV-1 expression in OM-10.1 and U1 cultures; whereas enhanced constitutive HIV-1 expression was observed in ACH-2 cultures at G2 + M. A 2 hour TNF-α treatment of all synchronized OM-10.1 cultures activated HIV-1 expression to a similar extent as unsynchronized cultures. In contrast, the extent of TNF-α-induced HIV-1 expression in ACH-2 S and G2 + M cultures and in the Ul G0/G1 culture was greater than that in unsynchronized control cultures. However, no delay in the initial response was observed. Thus, the influence of cell cycle on constitutive and induced HIV-1 expression varied in each cellular model and, therefore, may further relate to the different molecular mechanisms maintaining viral latency.
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Received November 6, 1996 Accepted January 15, 1997
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Roberts, B.D., Fang, G. & Butera, S.T. Influence of cell cycle on HIV-1 expression differsamong various models of chronic infection. Arch. Virol. 142, 1087–1099 (1997). https://doi.org/10.1007/s007050050144
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DOI: https://doi.org/10.1007/s007050050144