Abstract.
An integrated solid-phase spectrophotometry/flow injection analysis (FIA) method is proposed for the determination of the synthetic colorant matter Ponceau 4R (P4R) in the presence of its unsulfonated derivative 2-hydroxy-1-[(naphthalenyl)azo] naphthalene (N2N). The procedure is based on the measurement of P4R at λ = 508, followed by retention and preconcentration of the low level concentration of N2N on a C18 silica gel minicolumn and subsequent measurement of the absorbance of N2N at λ = 508 nm after its elution. The applicable concentration range, the detection and the relative standard were the following: for P4R, from 0.30 to 20.0 mg/L; 0.052 mg/L 1.1%; and for N2N, between 0.020 to 3.0 mg/L 0.003 mg/L and 1.1%. The method was applied to the determination of small amounts of N2N present in P4R in food and cosmetic products. Percentages of recovery between 95 and 105% were obtained in all instances. The method was applied satisfactorily to the determination of the compounds P4R and N2N in samples of sweets and cosmetic products when compared to results offered by a HPLC reference method.
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Received April 5, 2001. Revision July 25, 2001.
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Capitán-Vallvey, L., Valencia, M. & Arana Nicolás, E. Flow Injection Analysis with On-Line Solid Phase Extraction for Spectrophotometric Determination of Ponceau 4R and its Subsidiary Unsulfonated Dye in Sweets and Cosmetic Products. Mikrochim Acta 138, 69–76 (2002). https://doi.org/10.1007/s006040200011
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DOI: https://doi.org/10.1007/s006040200011