Abstract
Key message
Biomass allocation in Castanea sativa varies according to the environmental conditions. Specifically, leaf-to-sapwood area ratio is higher on sites with good water supplies and lower in water-stressed conditions.
Abstract
Ecological plasticity allows organisms to adapt and to cope with environmental conditions. This is a key trait for species with long live span, which will probably be more vulnerable to changing climate because of their lower adaptation potential via natural selection. We studied the case of the sweet chestnut tree, a naturalized forest species in many European mountain areas, where it grows on very different climates and sites. This raises the question of its adaptation capacity to very different environmental conditions. To test this hypothesis, we applied the pipe model approach for analysing the variation in the leaf-to-sapwood area ratio (A L:A S) in 82 chestnut trees growing in very different site conditions (e.g., water-stressed convex vs. water-rich concave sites). We used linear regression analyses to model the A L:A S relationship to environmental and dendrometric parameters. Results confirm that A L:A S is significantly higher when trees grow on good nutrient- and water-supplied concave sites with respect to water-stressed, convex sites. Chestnut trees are thus able to vary their biomass allocation between sapwood and leaves to adapt their hydraulic characteristics to the site conditions. Trees seem to react to water-stressed conditions by allocating more biomass in the sapwood in respect to the leaves. A L:A S may thus represent a useful indicator of tree species plasticity and their adaptation potential to different environmental and climate conditions.
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Introduction
Ecological plasticity results from the combination of genetic variability, environmental effects, and their interactions (Schlichting 1986; Martinez-Vilalta et al. 2009). This is a key adaptive trait for species with long generation times, which will probably be more vulnerable to changing climatic and environmental conditions because of their slower adaptation via natural selection (Hoffmann and Sgrò 2011). Understanding ecological plasticity is thus of paramount importance to predict tree species responses to climate change (e.g., Parmesan 2006). In particular, it is believed that in the future, the water resource may become an even more limiting factor in many ecosystems (e.g., Dai 2012) and species with a higher plasticity will have better chances to overcome future environmental changes.
At local scale, an important characteristic related to water-use efficiency is the ability of changing the biomass allocation between sapwood and leaves as a function of the specific site conditions and water availability in particular (Bonan 2002). In the case of a gradual rising of global temperature and the related water stress, increasing the resource allocation in the absorbing root system and in conductive sapwood may be a strategy to maintain a sufficient hydraulic conductivity to face the decreasing water availability. As postulated by Shinozaki et al. (1964a, b) and verified on different species (Vertessy et al. 1995; Cruiziat et al. 2002; Gehring et al. 2015), the leaf-to-sapwood area ratio (A L:A S) is constant for single portions of a tree crown and reflects the individual hydraulic characteristics of a tree, since it is the result of the energy and biomass investment in water absorbing, conducting, and transpiring tissues (e.g., Vertessy et al. 1995; Cruiziat et al. 2002). Furthermore, such physiological balance varies among trees of the same species as a function of the environmental conditions and local climate (Whitehead et al. 1984; Callaway et al. 2000; Martinez-Vilalta et al. 2009) and tends to be lower for individuals growing on poor and dry soils (Mazzoleni 1990; Mencuccini and Grace 1994; Sterck et al. 2008). Such a low A L:A S (which is reflected by an enhanced proportion of sapwood supplying water to leaves) associated with the leaf-specific conductivity may help trees growing in water-stressed conditions to maintain a sufficient water potential gradient and to avoid xylem cavitation (Maherali et al. 2004).
The chestnut tree (Castanea sativa Mill.) is a particularly interesting example in this matter, having the species being diffused and maintained in groves created by man in many mountainous regions of Europe over the past 2000 years (Conedera et al. 2004). In fact, such an active spread and active management has resulted in a broad diffusion of the species at a continental scale and in its establishment until the limits of the potential ecological range (Conedera et al. 2004). Despite such a heavy human imprinting, recent studies on the European chestnut diversity highlighted a high genetic and morphologic differentiation that is similar to other naturally dispersed European tree species, such as the deciduous oaks (Villani et al. 1992; Lauteri et al. 2004; Fernandez-Lopez et al. 2005; Mattioni et al. 2008; Martin et al. 2010; Beccaro et al. 2012; Mattioni et al. 2013). Furthermore, the need to adapt to very contrasting habitats resulted at continental scale in high physiological differentiation of water-use efficiency traits, such as the stomatal sensitivity to water stress limitations (Lauteri et al. 1997, 2004).
In this study, resting on the results of Gehring et al. (2015), we verify the hypothesis of existing variations in A L:A S among chestnut trees growing in different site conditions. We in particular analysed variations in A L:A S as a function of water supply-related environmental characteristics, such as microtopography, slope, aspect, solar insolation, and distance to next water channel, as well as dendrometric and other tree-related characteristics, such as height, diameter at breast height, crown height, and crown exposure to the light of trees.
Materials and methods
Study area
The chestnut area of Canton of Ticino covers ca. 15,000 ha at elevations below 1200 m a.s.l. (Fig. 1).
The geology of the area is dominated by siliceous rocks with small spots of limestone. Resulting soils are generally classified as haplic podzol or cryptopodzol, consisting in well drained, permeable, and unmottled sandy (clay fraction is usually less than 10 %) layers and a very consistent and stable organic matter component (Blaser et al. 2005). The main rooting zone covers the uppermost 30 cm of the mineral soil, whereas sparsely distributed roots are found at depths up to 2 m. Due to the soil permeability, water supply highly depends on the microtopography and tends to form a marked increasing gradient from the water-drained ridges to the water-enriched depressions (Blaser 1973).
The area is characterized by a mild (Insubrian) climate with mean annual precipitation around 1800 mm and mean annual temperature around 12 °C (MeteoSwiss climatic normals 1981–2010; http://www.meteoswiss.admin.ch/home/climate/past/climate-normals.html). During the wet and warm summers, periods without rain are interspersed with heavy precipitation events. When a prolonged dry period is accompanied by high summer temperature, drought-related damages, such as it was the case during the extreme hot summer season of 2003 (Spinedi and Isotta 2004) in the most exposed chestnut forests, can occur (Conedera et al. 2010; see also mean values of the aridity index calculated for the study site following UNEP (1992) in Fig. S1 of the Online Resource 1).
The chestnut (Castanea sativa) was first cultivated (and probably first introduced) in the area by the Romans nearly 2000 years ago (Conedera and Krebs 2008) and became an anthropogenic monoculture, occasionally interrupted by the presence of other broad-leaved species, such as Tilia cordata, Quercus petraea, Q. pubescens, Alnus glutinosa, Prunus avium, Acer spp., or Fraxinus spp. Due to its high economical value and broad and intense cultivation in the past (Krebs et al. 2012), chestnut trees are nowadays still present mostly as naturalized stands in areas with extremely varying microtopographic and water supply conditions.
A L:A S relationship in C. sativa
The postulate of the pipe model approach for C. sativa has been verified by Gehring et al. (2015) in the same study area. The study confirmed the constancy of the A L:A S ratio within a branch, highlighting, however, variations within the same tree between different types of branches that is architectural crown branches vs. epicormic shoots (reiterations). Reiterations in particular showed an enhanced sturdiness with respect to crown branches. Furthermore, within-tree differences exist among architectural crown branches as a function of the branch insertion height. For this reason, Gehring et al. (2015) introduced the notion of A L:A S of a hypothetical architectural crown branch at ground level (A L:A Sground) as a sampling height-independent parameter allowing to compare A L:A S ratios among different trees of the same species.
Since in our study we applied the leaf area-to-sapwood area approach on trees growing in extremely different environmental conditions and it was not possible to set a standardized sampling height. We, therefore, used both A L:A S and the branch height-transformed A L:A Sground as proposed by Gehring et al. (2015). In fact, without converting the effective measured AL:AS of the sampled branches to the theoretical A L:A Sground, any variation in response to environmental ecological factors could be masked by tree internal hydraulic constraints due to the different maximum heights of the sampled individuals. Using the branch height-transformed A L:A Sground, possible tree internal effects are minimized, allowing a direct comparison among trees displaying a great variability in height due to the differing growing site conditions (small trees on dry sites vs. tall trees on water-rich sites).
Sampling
Sample trees were selected within a radius of 10 km at altitudes ranging from 221 to 591 m a.s.l. to keep climatic and geological conditions as homogeneous as possible. On the contrary, tree selection targeted the highest possible gradient in terms of topography, from extreme water-stressed (e.g., trees growing on rocky ridges, ridges, and steep slopes) to extreme water-supplied (e.g., trees in terrain depressions or near a stream) sites. A total of 82 fully developed (adult) trees from natural regenerated stands were selected (Fig. 1), covering a height range from 1.5 (on extremely unfavourable sites) to 26.6 m (favourable sites) and a DBH range from 5 to 96 cm, respectively (Table 1). Each sampled tree was characterized using different dendrometric and site-related geo-physiographic (environmental) parameters (Table 2).
Data collection and analysis
From June 7 to August 13, 2009 and from June 16 to July 22, 2010, one (26 cases) or two branches (56 cases) per tree were collected from the top region of the crown, depending on its heterogeneity (symmetry and light exposure) and making sure that all leaves were fully developed and without damage from weather (e.g., hale), parasites or phytophagous insects. In particular, only crown branches were taken into consideration, avoiding epicormic shoots that have different hydraulic and A L:A S characteristics (Gehring et al. 2015).
Branches on tall trees were accessed using tree-climbing techniques, cut, and carefully lowered to the ground with a rope. The collected branches were processed in the same day as the cutting to preserve the original form and colour of the leaves. All leaves were then detached and digitized with a scanner (Xerox 9001 machine resolution 200 dpi, contrast +1, coloured photo quality) to accurately measure their area with the image-processing program Image Pro plus 6.0 (IPP).
Minimum and maximum branch diameters were measured with a calliper 1 cm before the nearest branching from the cutting point. The cross-sectional area was calculated from the two diameters using the ellipse formula. The presence of heartwood was checked on the dry branch samples using the soap bubble technique as described by Hoadley (1980). The soap bubble test consists in dipping the sample end where the sapwood area was measured in soapy water and blowing on the other sample end. The portion of the soapy cross section not forming bubbles, if any, is considered as heartwood. Where present, the proportion of heartwood was recorded and the correspondent area calculated using the ellipse formula as previously described. The sapwood area (A s) was then calculated by subtracting the heartwood portion from the cross-sectional area.
A L:A S was calculated dividing the total leaf area (A L) by the corresponding sapwood area (A S) for each branch. For trees where two branches were collected, A L:A S was calculated as the mean of both values. The transformation for calculating the theoretical ground-level A L:A Sground was achieved using the formula proposed by Gehring et al. (2015) for the study region, that is, A L:A Sground = A L:A S + 0.0282 heightbranch.
The data set was treated as unique, since no significant interannual A L:A S variation was detected (p > 0.05, non-parametric Mann–Whitney U test, data not shown).
The analyses were performed separately for both A L:A S and A L:A Sground. Data were first explored with univariate comparative analyses on the categorized explanatory variables (non-parametric Mann–Whitney U test). In the case of highly correlated variables (Pearson R > 0.6) expressing the same biological meaning, we retained the more pertinent ones from a physiological (ease of interpretation) and practical (easiness and precision to be collected in the field) point of view. Linear regression analyses were then used to model the relationship of the explanatory variables to A L:A S and A L:A Sground, respectively. To keep models simple and easy to interpret, we decided not to include interaction terms (Chambers et al. 1992). Since some dendrometric characteristics may already represent the tree response to the environmental conditions, we split the explanatory variables into two groups for the analysis: dendrometric and environmental parameters. We then performed the model selection on the two variable groups separately (dendrometric and environmental) as well on the whole variables set (dendrometric-environmental) (Table 2). Model multicollinearity was checked according to Belsley et al. (1980) that is looking for variation inflation factors smaller than 5 and condition indices smaller than 30. Only models without multicollinearity issues were retained choosing the best ones on the basis of the AICc coefficient (Akaike information criterion with a second-order correction for small sample size). We evaluated the goodness of fit of selected model by the means of the coefficient of determination (adjusted R 2), ranking then the relative contribution of each predictor according to the corresponding t values.
All statistical analyses were performed using the R statistical package version 3.2.2 (R Development Core Team 2015).
Results
The analyses performed with A L:A S and A L:A Sground showed similar results, the latter being always more significant.
A L:A S varied within the sampled trees by a factor of 8.4 (min 0.12, max 1.01) and the transformed A L:A Sground by a factor of 4.7 (0.29–1.35).
The distributions of the A L:A S and A L:A Sground values with respect to the environmental variables highlight the significant effect of combined microtopography (Fig. 2a, c), slope (Fig. 2b, d), and distance to the next water channel (see Fig. S2a,d in Online resource 1).
The best full A L:A S and A L:A Sground environmental model retains only composite microtopography (t value = 6.35, t valueground = 11.55) as explanatory variable and reaches an adjusted R 2 value of 0.33 and of 0.62 respectively (Table 3, Model E, E ground).
The distribution of the A L:A S and A L:A Sground values with respect to the dendrometric variables shows a positive significant effect with tree height (Fig. 3a, c), DBH (see Fig. S2b,e in Online Resource 1), and crown base height (see Fig. S2c, f in Online Resource 1) and negative with crown portion at light (Fig. 3b, d). In both the cases, the best full models based on dendrometric variables include tree height (t value = 2.69, t valueground = 11.62) and the crown exposure to light (t value = −2.69, t valueground = −2.84) and have an adjusted R 2 of 0.15 and of 0.65 for the model with A L:A S and A L:A Sground, respectively (Table 3, Model D and D ground).
The best full model with A L:A S as the response variable considering all variables (dendrometric and environmental) includes light exposure of the crown, composite microtopography, and slope (t value = −1.65, 5.55, and 1.17 respectively), reaching an adjusted R 2 of 0.31 (Table 3, Model D–E) and performing slightly better with respect to the exclusively environmental model (Table 3, Model E) and much better with respect to the dendrometric model (Table 3, Model D). On the other hand, the best full model with A L:A Sground as the response variable includes also the variable height (t value = 6.17), in addition to light exposure of the crown, composite microtopography, and slope (t value = −1.62, 5.36 and 0.98), reaching an adjusted R 2 of 0.74. The model performs better than the exclusively environmental or dendrometric models.
The variance inflation factors range from 1.01 to 2.17 in all models, and the highest condition index is equal to 7.9, suggesting that multicollinearity is not an issue and that the main environmental and dendrometric response variables are complementary and not collinear. Moreover, graphical examinations of linear models assumptions do not suggest any violation.
Discussion
Our data show that the investigated leaf-to-sapwood area ratios (A L:A S and A L:A Sground) decrease in water-stressed habitats and/or light-exposed conditions. Moreover, both the ratios show a significant decreasing trend between the small trees growing on extreme water-poor sites and the tall trees on water-rich sites. This is a strong evidence of an environmental-induced effect when considering that the within-tree untransformed A L:A S usually decreases with tree height as reported for the same species and the same study area by Gehring et al. (2015).
This change in biomass allocation may contribute to the maintaining of water flow from root to leaf by supplying greater hydraulic capacity per unit of leaf area, as postulated by the pipe model principle (Shinozaki et al. 1964a, b). It seems therefore reasonable to assume that the balance between water availability and evaporative demand is one of the main factors influencing the A L:A S ratio. Our results further strengthen the hypothesis that the water balance at the growing site is likely to influence the biomass partitioning of plants and trees in particular.
The obtained values’ range of leaf-to-sapwood area ratios along a gradient from supposed water-rich (concave position and nearer to waterbeds) to water-stressed sites (convex, steeper slopes) in chestnut trees is as double as the variability of the A L:A S values as reported for even-aged Populus euramericana (Mazzoleni 1990) growing on different site conditions.
The regulating role of microtopography on tree water balance is in line with the dominant soil characteristics as described by Blaser (1973) for the study region. The high drainage capacity of the podsolic sandy soils in the study area makes soil water availability highly dependent on the topography (Blaser et al. 2005) and partially on slope (as proposed by Grier and Running 1977). Trees growing on rocky ridges, ridges, and steep slopes may suffer from water deficit even in periods with regular rainfall because of the poor water retention capacity of the soil. This may be a critical issue especially during the phenological phase of leave unfolding and deploying which represent the first physiological process affected by water deficiency (Kramer 1983; Schulte et al. 1986; Mazzoleni and Dickmann 1988). These hypotheses are strengthened by the results of the environmental and dendrometric-environmental model selections, having in the best models microtopography as the most relevant variable (higher t value) and secondarily the terrain slope (Table 3, Model D–E).
The resulting multiannual equilibrium of A L:A S ratios not only reflects the microtopography-related soil water availability, but also the tree-growing conditions on the site as indicated by the relationship between the A L:A S ratios and the dendrometric parameters, such as height, DBH, and crown base height of mature trees. In fact, smaller and thinner trees were often situated on convex/drier sites, where they grow under low competition that enable them to keep the lower part of the crown active all lifelong.
Furthermore, the percentage of crown exposure to light gives a more complete picture of the overall water balance accounting for the evaporative stress caused by the direct sun exposure of the crown. Trees with higher crown exposure to light display an increased transpirational water loss and a correspondent lower leaf-to-sapwood area ratio (e.g., Grier and Running 1977; Jose and Gillespie 1996). Finally, from the results of the best dendrometric and dendrometric-environmental models, it seems that tree height and crown exposure to light are the most influent dendrometric variables, the first accounting for more variability in all A L:A Sground models (higher t values) and the second for the A L:A S models (higher or equal t values in the dendrometric model).
The observed response of biomass allocation to water availability and evaporative demand is consistent with the previous studies showing a dependency of the A L:A S ratio to climate (Mencuccini and Bonosi 2001; Poyatos et al. 2007; Martinez-Vilalta et al. 2009), to site conditions (Mazzoleni 1990; Mencuccini and Grace 1994; Sterck et al. 2008), as well as to evaporative demand (Waring et al. 1982; Mencuccini and Grace 1994) or vapour pressure deficit (DeLucia et al. 2000).
Regarding the branch height-transformed A L:A Sground, this study confirmed the utility and the validity of the approach proposed by Gehring et al. (2015) for comparing trees growing in extremely different site conditions. The analysis of A L:A S and A L:A Sground showed qualitatively similar results, but models with A L:A S had a much smaller explicative power (R 2 < 0.34) compared to those with A L:A Sground, which displayed high coefficients of determination (R 2 > 0.62). This lets us assume that the applied transformation does not skew the results but remove the masking effect due to the great variability in the sampling height of the branches.
From an applied point of view, the results presented in this study may improve our understanding of the stool uprooting phenomena in over-aged chestnut coppices as reported by several authors for northern Italy and southern Switzerland (e.g., Pividori et al. 2008). As pointed out in earlier studies (Vogt et al. 2006; Conedera et al. 2009), uprooting stools are mostly located in concave, water-rich microtopographies, where our study found higher values for the A L:A S and A L:A Sground ratios. It appears reasonable to postulate that the unbalance between oversized aerial biomasses and root systems causing the uprooting is reached sooner or prevalently in water-rich concave sites, what may confirm the hypothesis of a proportionality between the leaf-to-sapwood area ratio and the above-to-below ground biomass ratio (Mazzoleni 1990). Using the A L:A S and A L:A Sground ratios as proxies for above and below ground biomass partitioning has to be further investigated and confirmed, but could result in an important factor to be considered for the assessment and modelling of stand and slope stability in mountain areas (Schwarz et al. 2010; Schwarz et al. 2012).
Conclusions
The relationship (A L:A S) has been confirmed to be a useful instrument to assess the ecological plasticity and thus the ability of a species to establish in sites with different characteristics, and differences in water availability in particular. Furthermore, the theoretical A L:A Sground represents a suitable measure for making the A L:A S ratio a comparable hydraulic parameter among individuals of the same tree species. Further studies are on contrary necessary to verify if the ratio between leaf and sapwood areas may also be considered a proxy for the relationship between the aerial and the ipogeic biomass of the corresponding trees.
Author contribution statement
PGB, CM, and MS originally formulated the concept of the study and with GE decided the sampling design. KP conceived the leaf area measurement and the image analysis and PGB developed and managed the database. GE conducted the fieldwork and performed the statistical analyses with PGB. GE wrote the manuscript, which was revised by PGB, MS, and CM. ZM computed the analysis for the aridity index. CM coordinated the research project and provided funds.
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Acknowledgments
Our heartfelt thanks go to Franco Fibbioli, Nora Buletti, and Susan Lagger for their help during data collection.
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Gehring, E., Pezzatti, G.B., Krebs, P. et al. The influence of site characteristics on the leaf-to-sapwood area relationship in chestnut trees (Castanea sativa Mill.). Trees 30, 2217–2226 (2016). https://doi.org/10.1007/s00468-016-1447-9
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DOI: https://doi.org/10.1007/s00468-016-1447-9