Abstract
The impacts of disturbance on biodiversity and distributions have been studied in many systems. Yet, comparatively less is known about how lichens–obligate symbiotic organisms–respond to disturbance. Successful establishment and development of lichens require a minimum of two compatible yet usually unrelated species to be present in an environment, suggesting disturbance might be particularly detrimental. To address this gap, we focused on lichens, which are obligate symbiotic organisms that function as hubs of trophic interactions. Our investigation was conducted in the southern Appalachian Mountains, USA. We conducted complete biodiversity inventories of lichens (all growth forms, reproductive modes, substrates) across 47, 1-ha plots to test classic models of responses to disturbance (e.g., linear, unimodal). Disturbance was quantified in each plot using a standardized suite of habitat quality variables. We additionally quantified woody plant diversity, forest density, rock density, as well as environmental factors (elevation, temperature, precipitation, net primary productivity, slope, aspect) and analyzed their impacts on lichen biodiversity. Our analyses recovered a strong, positive, linear relationship between lichen biodiversity and habitat quality: lower levels of disturbance correlate to higher species diversity. With few exceptions, additional variables failed to significantly explain variation in diversity among plots for the 509 total lichen species, but we caution that total variation in some of these variables was limited in our study area. Strong, detrimental impacts of disturbance on lichen biodiversity raises concerns about conservation and land management practices that fail to incorporate complete estimates of biodiversity, especially from ecologically important organisms such as lichens.
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Introduction
It is widely recognized that disturbance plays a major role in determining species diversity, distributions, and functions (Hutchinson 1953; Connell 1978; Johst and Huth 2005). Approaches to understanding the impacts of disturbance have included examining effects of variables across trophic levels (Wootton 1998), spatial scales (Witman et al. 2008; Limberger and Wickham 2012), and temporal scales (Svensson et al. 2007; Zemanová et al. 2017). Others have explored how disturbance affects functional diversity and ecosystem services (Biswas and Mallik 2010; Thom and Seidl 2016), whether choice of diversity measure matters (Mackey and Currie 2001; Johst and Huth 2005; Svensson et al. 2007), and how species traits such as dispersal respond to disturbance (Johansson et al. 2013). This has yielded a nuanced understanding of how, and in what contexts, disturbance explains spatial variation in diversity and function across organisms and ecosystems.
Despite the above advances, few large-scale studies have examined how disturbance–natural or anthropogenic–impacts the lichen symbiosis. Specifically, the establishment and development of a lichen requires that a minimum of two compatible, yet often unrelated symbionts, be present in any given environment. This suggests that, in contrast to some organisms, lichens and other obligate symbionts may be especially sensitive to disturbance because their life strategies are initially constrained by biotic interactions. Further, such organisms may respond differently to disturbance in comparison to non-obligate organisms. For example, classical disturbance models predict responses based on tradeoffs between disturbance tolerance and competitive ability (Hutchinson 1953; Connell 1978; Huston 1994, 1979; Wilson 1994; Violle et al. 2010). However, obligate symbiotic diversity and distribution, such as that of lichens and many other organisms, may be governed more strongly by intrinsic biotic factors such as whether or not all symbionts are present in the environment at the earliest stages of development (Honegger 2012; Tripp et al. 2016).
If the above is true, theoretical underpinnings of classical models, which serve as umbrellas under which the interplay among disturbance, diversity, and abundance is interpreted (Shea et al. 2004; Sheil and Burslem 2013) may not fully explain patterns in lichens and other obligate symbiotic organisms in nature. Understanding whether and how lichens respond to disturbance has widespread implications considering the proliferation of studies documenting the prevalence of microbiomes and symbiomes, or co-evolving sets of unrelated symbionts (Röthig et al. 2016; Tripp et al. 2017; Brodie et al. 2017).
Lichens are obligate symbiotic organisms composed of a minimum of one fungus and one alga or cyanobacterium (Brodo et al. 2001). A given lichen also often harbors numerous other symbionts including bacteria, additional algal genotypes, and additional fungi (Arnold et al. 2009; Bates et al. 2012; U’Ren et al. 2012; Fleischhacker et al. 2015; Muggia et al. 2016; Chagnon et al. 2016; Fernandez-Mendoza et al. 2017). Thus, lichens function as hubs of trophic interactions, represent a remarkable symbiotic life form and, serve as a source for evolutionary innovation not achieved elsewhere in the fungal tree of life (Honneger 1991; Lutzoni et al. 2001). From a functional perspective, lichens exist via diverse growth forms, display complex morphologies and reproductive modes, and contribute a broad array of ecological services including biogeochemical cycling, biomass production, pollutant sequestration, decomposition, soil formation, and habitat or nutrition sources for an untold diversity of organisms (Szczepaniak and Biziuk 2003; Cornelissen et al. 2007; Asplund and Wardle 2017). From a biological diversity perspective, lichens are often among the most diverse eukaryotic organisms in a given area, following insects, vascular plants, and non-lichenized fungi (e.g., DLIA 2017).
For nearly two centuries, disturbance has been recognized as having a major role in shaping patterns of lichen diversity and abundance. However, knowledge of lichen disturbance ecology has developed in three focal areas. First, the majority of studies have focused on subsets of lichen diversity, such as macrolichens, foliicolous lichens, or specific species of lichens (Mistry 1998; Dynesius and Zinko 2006; Benítez et al. 2012; Barry et al. 2015; Arsenault and Goward 2016; Tarasova et al. 2017). In contrast, few studies have incorporated data derived from total lichen diversity, with most studies completely excluding microlichens that can comprise more than half the total diversity in an area (e.g., Tripp 2015; Lendemer et al. 2016). Second, research to date has emphasized responses to catastrophic disturbances (Mistry 1998; McMullin et al. 2008; O’Bryan et al. 2009; Ellis and Coppins 2010; Lundström et al. 2013; Bartels and Chen 2015), land management practices (Zemanová et al. 2017; Ray et al. 2015), and pollution (McCune et al. 1997; Ellis and Coppins 2010; Shrestha and St. Clair 2011). In contrast, much less is known about the impacts of milder to moderate forms of disturbance such as patchiness, extent and quality of native habitat, and forest maturity (but see Johansson et al. 2012; Pastore et al. 2014). Third, prior studies have not dissected individual components of disturbance in natural systems via analyses of a suite habitat quality variables in addition to standard ecological metrics and environmental variables. This is particularly important given that extensive work has shown that habitat diversity and complexity, together with climate, are important factors that drive the distributions of biodiversity (MacArthur 1972; Currie 1991; McCain 2009; Kessler et al. 2011).
Understanding of the interplay between lichen diversity and disturbance has developed in the absence of taxonomically comprehensive studies spanning the dimensions of disturbance and environment across full gradients typically found in natural systems. Here, we use a new regional-scale dataset to test whether and how anthropogenic disturbance explains variation in lichen species diversity in a temperate biodiversity hotspot. The dataset spans 47 1-ha plots in the southern Appalachian Mountains of eastern North America and includes more 4000 occurrences representing 509 phenotypically delimited lichen species (see “Materials and methods”), in addition to data for woody plant diversity, forest density, rock density, climate and geographical factors, ten components of habitat quality.
Materials and methods
Study plots
To assess impacts of disturbance and additional biotic and abiotic factors on lichen diversity, we inventoried total lichen biodiversity across 47 one-hectare (ha) sites in the southern Appalachian foothills of northern Alabama (Fig. 1). Site selection spanned several axes of disturbance plus a range of additional variables (tree and rock substrate density, woody tree diversity, elevation, aspect, slope). These plots were replicated across the above axes as best as possible, following Fierer et al. (2011), McCain (2004, 2009), and McCain and Grytnes (2010). During the site selection process, attempts were made to hold uniform several explanatory variables to minimize potentially confounding effects of extreme within-site variation. For example, in a given plot, we sampled only one aspect (e.g., east-facing), attempted to minimize variation in slope (e.g., 10˚ throughout the 1 ha), and did not traverse major shifts in habitat type (e.g., dry ridgetop forest vs. wet riparian forest).
Biodiversity inventory
In each 1 ha plot, a taxonomically comprehensive lichen inventory (all growth forms, all substrates, all reproductive modes) was implemented via expert-based surveys in which each species encountered by a collector was vouchered. These methods have been shown more effective at capturing total diversity than plot- or transect-based methods as well as inventories by non-experts (Coddington et al. 1991; Sørensen et al. 2012). All 47 plots were sampled until total species diversity was vouchered, as determined by the primary collectors (collection times varied between 10 min in highly degraded sites to 110 min in less disturbed sites). Using species counts per plot, we calculated an accumulation curve via the specaccum function in the vegan package (http://vegan.r-forge.r-project.org) in R. For each voucher specimen, precise geographic locality, habitat, substrate, and other ecological information were recorded. Two individuals (Lendemer, Tripp) completed all taxonomic inventories. Collection of ecological data (see below) was spearheaded by McCain assisted by Tripp, Lendemer, Anderson Stewart, and Hoffman.
Voucher specimens were returned to a temporary, traveling laboratory for preliminary identifications. Unique identifiers were assigned to each collection and its subcollections (e.g., DNA vouchers, photographs). Specimens were curated (e.g., soil stabilized), thin-sectioned to study anatomical features (e.g., ascospore shape, size), and phenotyped for taxonomically important secondary metabolites using a traveling Thin Layer Chromatography lab [TLC methods following Lendemer (2011)]. All specimens were identified using regional keys (Lendemer et al. 2013, 2016; Tripp and Lendemer 2019a, in press) that follow current concepts of phenotypically characterized lichen species (Esslinger 2016, for the most part), many of which have been included in molecular phylogenetic studies and found to be monophyletic. Identifications were further refined at home institutions of J. Lendemer (New York Botanical Garden, NY Herbarium) and E. Tripp (University of Colorado, COLO Herbarium). Vouchers were collected in duplicate, are permanently deposited at NY and COLO, and resulting data are available at: NYBG’s Starr Virtual Herbarium (http://sweetgum.nybg.org/science/vh/), COLO’s internal database (https://botanydb.colorado.edu/index.php), SEINet (http://swbiodiversity.org/seinet/), and iDigBio (https://www.idigbio.org/).
Phenotypic (morphological) species delimitation
Recognition of species on the basis of phenotypes vs. genotypes varies widely in mycology and is oftentimes group-specific (Balasundaram et al. 2015; Yahr et al. 2016). Species delimitation methods, moreover, vary as a function of density of cryptic diversity (Miadlikowska et al. 2018). In lichen-forming fungi, examples of species that cannot be delimited and recognized using phenotypic (including chemical) characters appear to be rare (for example, the 800 square miles comprising Great Smoky Mountains National Park–the most lichenologically diverse national park in the United States–contains nearly 1000 species, all of which can be identified using standard phenotypic-based methods of delimitation; Tripp and Lendemer 2019a, in press). In fact, in lichens, the vast majority of molecular studies have led to taxonomic redelimitation of species using previously overlooked or inconspicuous phenotypic characters (Yahr et al. 2016; Lücking et al. 2017); this applies even to instances where species were originally purported to be cryptic (e.g., Ossowska et al. 2018). Thus, we assert that the phenotypically delimited species are a reasonable approximation of the actual taxonomic diversity of lichens in a given biogeographic region (Leavitt et al. 2016; Yahr et al. 2016; Magain et al. 2018; Tripp and Lendemer 2019a, in press), as is the case for many other well-studied group of organisms that can be readily examined with a microscope.
Disturbance assessments
Northern Alabama hosts a spatially mosaicked, broad spectrum of habitat qualities ranging from exceptionally disturbed to moderately intact to near-virgin forests that have seen little impact in recorded history. Examples of exceptionally disturbed habitats include cultivated cornfields, immature forest regrowth following clearcutting, and paved parking lots. Examples of moderately intact habitats include the majority of extant, primarily native ecosystems in this portion of the state (e.g., lands managed by the U.S. Forest Service, and Alabama state lands under jurisdiction of Forever Wild, including Wildlife Management Areas). Examples of near-virgin forests include old-growth box canyons in the Sipsey Wilderness (Bankhead National Forest) and small subsets of Little River Canyon National Preserve and Skyline Wildlife Management Area.
We quantified disturbance on a scale of − 2 (lowest habitat quality [HQ], or most disturbed) to 95.2 (highest HQ, or least disturbed) using the native vegetation assessment methods of Parkes et al. (2003; note this scale allows for a minimum value that is negative). The HQ of each plot was based on the sum of 10 metrics (value range for each in parentheses; see Fig. 2) and incorporated both site-specific as well as landscape context metrics including: percentage of mature trees (0–10), percentage of native tree canopy cover (0–5), percentage of native understory and degree of anthropogenic modification (0–25), percentage of weed cover (0–15), percentage of recruiting native trees (0–10), percentage of native, expected litter quantity (0–5), percentage of native, expected coarse woody debris (0–5), habitat fragment size (0–10), degree of fragmentation of surrounding hectares (− 2 to 5.2), and distance to nearest core area (0–5) based on Parkes et al. (2003). All disturbance scores were quantified in the field at the time of plot sampling.
Forest diversity, density, and rockiness assessments
Because total tree diversity and density of woody vegetation present can impact diversity of other organisms, especially sessile taxa such as lichens that utilize trees as primary substrates, we also quantified these factors in each plot. First, a total checklist of all woody, vascular plant diversity (trees and shrubs) was compiled for each plot using existing knowledge and supplemented by standard identification tools, as needed (Weakley 2015). These data were analyzed (see below) with and without inclusion of the shrub component. Second, we established a 10-square meter (m2) representative subplot within which we quantified the total number of trees > 10 cm diameter at breast height (DBH) as well as the diameter and species identification of each tree. Finally, because rocks represent an important substrate that can host highly unique communities of lichens (Flock 1978; Brodo et al. 2001; Henson et al. 2013; Tripp 2015), we assessed the density of exposed rock at each site on a qualitative scale ranging from 1 to 10.
Environmental variables
Numerous climatic and geographic variables are associated with taxonomic diversity, often with high correlation (e.g., Hawkins et al. 2003; Currie et al. 2004; Dynesius and Zinko 2006; Ellis and Coppins 2010; McCain and Grytnes 2010). For the southern Appalachians, important potential variables related to overall lichen diversity include temperature, precipitation, net primary productivity (NPP), elevation, slope, and aspect. Using geographic coordinates of each plot, we extracted elevation from a digital elevation model (Shuttle Radar Topography Mission (SRTM), NASA, resolution: 90 m) in ArcGIS, whereas we estimated the slope and aspect while sampling the plot. We used mean annual temperature and precipitation from the PRISM 30-year normals (resolution: 800 m2, 1981–2010), and mean NPP using MODIS estimates (MOD17A3, resolution: ~ 1 km2, 2000–2013), extracted for each plot using ArcGIS.
Statistical analyses
To assess the fit of disturbance models to our data, we compared linear, quadratic, and cubic relationships using AICc weights to extract relationships between lichen diversity and disturbance. To determine which variables were most associated with lichen diversity across these 47 sites, we ran forward, stepwise, multivariate linear regressions for all quantitative variables: elevation (m), disturbance, NPP (gC/m2/yr), precipitation (mm), rock cover, slope, temperature (°C), tree diameter (sum DBH), tree quantity (count), and tree richness. For stepwise variable inclusion, we used the minimum BIC cutoff value (models were consistent for BIC, AICc, and p value cutoff values except that the latter two included more variables, but additional variables were consistently non-significant individually when included). Model fit was assessed with r2 values and variable strength with p values. Several of our abiotic and biotic variables are correlated (e.g., temperature and elevation, disturbance and number of trees; see Appendix 2 for a correlation matrix and additional analyses), thus stepwise multivariate regression was chosen to simultaneously assess variable fit and collinearity. But, we also compared best stepwise multivariate regression models with and without disturbance to assess relative importance of the collinear variables, and we conducted a multivariate partial least squares test to detect the key variables that should be included in the model despite collinearity (see Appendix 2 for further details).
For plot orientation, assessed qualitatively (flat vs. facing east, west, south, north), we used ANOVAs to test for differences in lichen diversity as well as disturbance. Additionally, we ran the same multiple regression methods employing additional variables for functionally contrasting groups of lichens [sexually (via fungal-only ascospores, which must later encounter at minimum a new photobiont partner to complete the reproductive process) vs. asexually (via lichenized propagules that co-disperse both the fungal and algal partners, and likely other symbionts) reproducing lichens, microlichens vs. macrolichens, and four dominant growth forms consisting each of a minimum of 20 taxa (crustose, foliose, fruticose, squamulose)] to detect potential biological differences among these groups. Mode of reproduction was determined as the primary mode representative for each species (e.g., some lichens reproduce asexually via lichenized propagules for most phases of their life history but are known to pass through brief, sexual phases; these species were scored as asexual; in contrast, sexually reproducing species are not known to manufacture asexual, lichenized propagules, Tripp and Lendemer 2018). Statistical analyses were conducted using JMP Pro 13.0 (2016 SAS Institute Inc.).
Results
Across the 47 sites, we documented a total of 509 lichen species. Overall the biota was dominated by microlichens [316 taxa (62%) vs. 193 macrolichens (38%)] while there were slightly more sexually reproducing species than asexually reproducing species [206 asexual (40%) vs. 294 sexual (58%); 9 species unknown]. Crustose lichens were also the most diverse, with 302 species, compared to 129 foliose, 25 fruticose, and 42 squamulose lichens (10 lichenicolous taxa plus 1 unknown not included in analyses; Electronic Supplemental Material Appendix 1). The accumulation curve showed that over half of all sampled species were documented after inventory of the first 12 plots but that even after the total number of plots were sampled, accumulation had yet to fully taper off (Fig. 3). The sites spanned a broad distribution of habitat qualities, ranging from 0.00 to 95.2, and specific species responded differently to disturbances (Table 1; Electronic Supplemental Material Appendix 1). Most of the 10 habitat quality metrics increased with habitat quality, but with varying degrees of overlap and strength of importance (Fig. 2). For all lichens, we recovered a strong, unequivocally linearly increasing relationship between the number of lichen species and decreasing disturbance (Fig. 4: All, r2 = 0.645, AICc weights: linear = 0.708, quadratic = 0.222, cubic = 0.070).
The best stepwise, multivariate regression model for the distribution of lichen diversity across all sites included only disturbance (r2 = 0.645, p < 0.0001; Appendix 2). Several of the explanatory variables were correlated, although most were not strong (r < 0.30; see correlation matrix in Appendix 2). Thus, the total model, including all 10 biotic and abiotic variables only slightly improved the model (r2 = 0.696) and the complete model without disturbance was a poorer fit (r2 = 0.539, p < 0.001). To further assess collinearity, partial regression statistics detected that disturbance residuals were minor, whereas the residuals of the collinear variables were large and still had considerable variation unexplained. Disturbance and rock cover are negatively correlated (r = − 0.4220), and disturbance and several of the tree variables were correlated (Appendix 2), which are likely causal as humans do not have many uses for areas of large rocky outcrops and disturbance negatively influences trees as well as lichens.
The best models for microlichen, macrolichen, and asexual lichen diversity, when analyzed separately (Fig. 4, r2 = 0.635, p < 0.0001; r2 = 0.580, p < 0.0001, r2 = 0.533, p < 0.0001, respectively), were strongly related only to disturbance. However, sexual lichen diversity was best predicted by decreasing disturbance (Fig. 4, p < 0.0001) as well as a trade-off between precipitation (p = 0.0181) and rock cover (Fig. 5, p = 0.037, overall model r2 = 0.707, p < 0.0001). With lower rock coverage, sexual lichen diversity was found to increase with increasing precipitation whereas with greater rock coverage, diversity decreased with increasing precipitation. Nonetheless, this precipitation–rock cover interaction explained only a small amount of variation (0.075) beyond disturbance alone (r2 = 0.632).
Among the four dominant lichen growth forms, disturbance was again the best predictor of diversity. It was the only variable in the best models for crustose and foliose lichens individually (r2 = 0.637, p < 0.0001, r2 = 0.529, p < 0.0001, respectively) whereas the best models for less diverse growth forms (i.e., fruticose and squamulose lichens) also included a second variable beyond disturbance as the strongest variable. However, these models were weaker (r2 = 0.391, p < 0.0001, r2 = 0.359, p < 0.0001, respectively). Fruticose lichens increased with decreasing disturbance (p = 0.005) and rock cover (p = 0.019) whereas squamulose lichens increased with decreasing disturbance (p < 0.0001) and decreased with overall quantity of tree bark (summed DBH, p = 0.003).
Finally, we found that flat sites had fewer lichen species than sites with slopes facing any direction (Fig. 6a, ANOVA r2 = 0.403, p = 0.002). However, when disturbance among sites was compared across aspects, the most disturbed sites were flat sites (Fig. 6b, ANOVA r2 = 0.620, p = 0.002).
Discussion
Our data strongly support a linear relationship between lichen diversity and habitat quality and disturbance. This result was recovered both from analysis of our full dataset as well as subsets of the data parsed by important lichen traits such as growth form, reproductive mode, and substrate (all r2 values > 0.5). Beyond disturbance, our study recovered minor support for additional factors in explaining lichen biodiversity including effects of precipitation (sexual species), rock cover (sexual species, fruticose species), and tree density (squamulose species). Additionally, several of our explanatory variables were correlated (Appendix 2). In particular, the relationships between disturbance and several forest characteristics (e.g., number of trees, canopy coverage) are likely causal and, therefore, also implicated in the potential mechanisms of lichen decline with human disturbance. Similarly, but in reverse, human disturbance declines in areas of high rock coverage, thus humans have less impact on such regions.
The disturbance–diversity relationship in lichens
There is extensive evidence documenting that lichen diversity declines with increasing disturbance (Lücking 1995; Ellis and Coppins 2010; Benítez et al. 2012; Nylén and Luoto 2015; Ardelean et al. 2015; Tarasova et al. 2017; Zemanová et al. 2017). However, while this connection is well-established, prior studies have almost exclusively focused on subsets of total lichen diversity (e.g., macrolichens or epiphytes) and treated disturbance either qualitatively by assigning broadly defined classes (Lücking 1995; Rivas Plata et al. 2008; Benítez et al. 2012) or utilized time since disturbance as a proxy for degree of disturbance (Hestmark et al. 2007; O’Bryan et al. 2009; Ellis and Coppins 2010; Bartels and Chen 2014, 2015; Nelson et al. 2015; Arsenault and Goward 2016; Tarasova et al. 2017; Zemanová et al. 2017).
The present study provides a new lens with which to view lichen disturbance ecology, as it appears to be the first to directly examine total lichen species richness along a disturbance gradient wherein disturbance was quantified using a standardized suite of habitat quality metrics (Fig. 2). Further, the present study emphasized patterns across a large regional scale in the context of a temperate biodiversity hotspot for both lichens and vascular plants. Our results support the broad conclusion of earlier studies that lichens are negatively impacted by disturbance and decreased habitat quality. We also found that beyond the primary effect of disturbance, there are secondary effects of variation in microhabitat features including annual precipitation and rock cover. Nonetheless, this study highlights that the relationships between lichen diversity, disturbance, and habitat quality are exceptionally strong across the full complement of lichen diversity in this study region. That is, regardless of size, reproductive mode, growth form and ecology, the diversity of lichens is strongly driven by disturbance and habitat quality.
What factors may have contributed to the linear relationship between disturbance and lichen diversity? Huston (2014) hypothesized that exceptionally high or exceptionally low levels of productivity could drive such patterns (e.g., through low colonization/developmental rates or high mortality/competitive exclusion). However, net primary productivity (NPP) did not explain the variation in diversity observed in this study, although we caution that Alabama is not particularly variable in NPP (average = 7152, range = 6102–10,419 g C m−2 yr−1). We question whether lichens may be less subject to classic tradeoffs (Connell 1978; Wilson 1994; Violle et al. 2010) and more strongly driven by availability of resources (Death 2002).
Lichens are by definition autotrophic organisms, and thus are not primarily limited by carbon availability in their environments (Honegger 1998; Cornelissen et al. 2007). Rather than abiotic limitations, the single most important resource required for lichens to colonize a new landscape is likely the availability of suitable symbiotic partners. Due to the diverse reproductive modes of lichens, these partners can be transmitted vertically, horizontally, or via a combination of means depending on species-specific factors (Dal Grande et al. 2012; Muggia et al. 2014; Tripp and Lendemer 2018; see also Chagnon et al. 2016). While many lichens produce asexual propagules that co-disperse the complement of symbionts required to form a lichen, a larger proportion reproduce sexually and must establish new contact with at minimum a suitable photobiont to yield further development (Tripp and Lendemer 2018). For example, in the present study, 40% of lichen species co-disperse their symbionts asexually while 58% disperse sexual ascospores. This mirrors broader continental scale patterns in North America [> 70% of all North American lichens reproduce sexually (E. Tripp and J. Lendemer, ms in prep.)].
Following symbiont availability, lichen colonization is expected to be limited by substrate availability and variety (i.e., presence and type of woody substrates or rocks) given that substrate preferences of many lichens relate to a narrow range of chemical and physical characters (Barkman 1958; Wolf 1994; Wolseley and Aguirre-Hudson 1997; Cáceres et al. 2007; Nelson et al. 2015; Tripp 2015; Jüriado et al. 2017; McDonald et al. 2017; Resl et al. 2018). Subsequent to initial colonization and establishment, the most important factors governing later stages of lichen development are likely to involve physiological constraints and tolerance of the abiotic environment and microclimates including temperature, precipitation, airborne (or waterborne) nutrients, and air quality (McCune et al. 1997; Dynesius and Zinko 2006; Ellis and Coppins 2010; Nelson et al. 2015), in addition to ongoing biotic interactions experienced by a given individual throughout its lifetime (e.g., competition, disease dynamics).
Taken together, it seems likely that two broad classes of factors, biotic and abiotic, impact lichen biodiversity at different life stages: colonization (dependent on symbiont availability and density; see Tretiach et al. 2013) and subsequent development (dependent on abiotic factors in addition to ongoing biotic interactions). If true, this complexity presents challenges to the hypothesis that any single resource may set an upper limit on lichen diversity. In the present study, symbiont availability was not examined, but this ‘biotic limitation’ hypothesis presents an exciting avenue for further study (K. Keepers et al., ms in prep.).
Limited additional factors predict lichen diversity
Our key finding that habitat quality and disturbance drive patterns of lichen diversity was tempered by limited demonstration of the potential relevance of additional variables modulating natural lichen diversity. For instance, we found precipitation and rock cover impacted sexual lichens, and rock or tree cover helped explain species richness of taxa with fruticose and squamulose growth forms.
That sexual species diversity increased with precipitation at low rock cover but declined with precipitation for higher rock cover suggests an interesting tradeoff that may involve several different contributing factors including habitat diversity (with or without rocks) and competitive exclusion imposed by bryophytes (typically more abundant at higher, wetter elevations, e.g., Dynesius and Zinko 2006). This, however, does not lend insight into why such a tradeoff should impact sexual but not asexually reproducing lichens. This tradeoff, if accurate, may involve limitations of sexual spore dispersal or colonization in rocky, wetter habitats compared to the mitotic diaspores of asexually reproducing species that are dispersed together with the full complement of symbionts (Tripp and Lendemer 2018).
Although not yet empirically demonstrated, there likely exist correlations between (1) mode of reproduction and growth form for the two most species-rich groups of lichens, foliose and crustose lichens, as well as (2) competitive interactions between these two growth forms as a function of precipitation and substrate. Results from ongoing research (E. Tripp and J. Lendemer, ms in prep.) have already revealed that foliose species are overrepresented in regions of North America with extensive hardwood forest cover (such as the Appalachian Mountains) whereas crustose species are overrepresented in regions with extensive rock cover (such as the Rocky Mountains). At the same time, crustose species tend to reproduce sexually whereas foliose species tend to reproduce asexually. Thus, high and typically wet elevations in eastern North America may be dominated by foliose species because of physiological constraints such as higher water demands (larger, thicker thalli) to the exclusion of crustose species. Correspondingly, lower and drier environments in this region may be more conducive to the establishment and development of crustose species that have lower water demands, especially considering that crustose lichens tend to be overrepresented on rocks. Better understanding of functional traits (cf. Nelson et al. 2015) across a large complement of North American lichens would likely shed new light on distributions of species and their traits at regional and local scales.
For fruticose species, the best model for predicting richness included disturbance as well as high rock cover whereas for squamulose species, the best model incorporated disturbance together with a low number of large trees. This may reflect open habitat associations, but note that both models were weaker on average than were all models for other lichen groups that included only disturbance. Additionally, these two growth forms together comprise only a small fraction (13%) of our overall dataset of 509 species, which was dominated by crustose (59%) and foliose (25%) species.
We also failed to find evidence that tree diversity, total number of trees per plot, average tree DBH per plot, plot elevation, slope, temperature, or net primary productivity impacted lichen biodiversity. But as noted above, the tree variables are also positively and linearly related to habitat quality, thus decline with disturbance as do lichens (Fig. 2; Appendix 2). They alone are not better explanatory variables than disturbance, but are casually linked to disturbance, and thus lichen diversity patterns. In contrast, our analyses did recover a significant effect of plot orientation: flat plots were less diverse than those on slopes facing any cardinal orientation. Flat plots were, however, also the most disturbed, suggesting a correlation: flat areas are more readily disturbed by humans and a need to incorporate more data from disturbed plots on slopes. That so many of the above factors did not explain patterns of species richness at first seems puzzling given the preponderance of studies that have highlighted their importance, especially that of temperature, elevation, and productivity (Currie 1991; McCain and Grytnes 2010; García-López et al. 2012). However, variability in most of these abiotic environmental factors was limited in the present dataset due to the spatial proximity of the plots in northern Alabama. An expanded study that spans larger gradients in temperature, precipitation and NPP is needed to more fully understand their relative importance to patterns of lichen diversity (e.g., Ellis and Coppins 2010), such study is currently underway by the authors (C. McCain et al., in progress).
Ecological thresholds
Data from the present study provide an opportunity to understand specific attributes of lichens such as tolerance to disturbance, commonality, and rarity. A total of seven lichen species were found both multiple times in our study and consistently occupied only the most disturbed habitats (i.e., HQ < 50%, Table 1; Electronic Supplemental Material Appendix 1). Of these, all reproduce asexually except one and all are crustose except one, which is microfoliose. This suggests that both traits, asexual reproduction and a crustose growth form, are conducive to disturbance tolerance. Other disturbance-tolerant species were found only in the most disturbed plots but found only once in our study, and finally an assemblage of additional species was found in highly disturbed plots in addition to relatively undisturbed plots (Table 1; Electronic Supplemental Material Appendix 1).
Seventeen species comprised the most common taxa (i.e., present in 25 or more plots), and these are a mix of foliose, crustose, sexual and asexual lichens. They are, however, considered among the most common lichens in eastern North America (Table 1; Electronic Supplemental Material Appendix 1). These taxa are characterized by broad ecological thresholds and are found frequently as a cohort in a wide variety of habitats (Brodo 1961; Dibben 1980; Brodo et al. 2001; Hinds and Hinds 2007; Lendemer et al. 2013; Muscavitch et al. 2017). In contrast, nearly a third of lichens in our dataset (144 of 509 species, or 28%) were found and collected in the field only once. This percentage is on par with numbers derived from other lichen biodiversity hotspots, such as The Greater Sonoran Desert, Great Smoky Mountains National Park, and the Dare Regional Biodiversity Hotspot (Nash 2002; Lendemer et al. 2013; Lendemer and Allen 2014; Lendemer et al. 2016; Tripp and Lendemer 2019a, b in press). The species that were located only once included several that are either at the edges of their biogeographical ranges, are rare to begin with, or both (Table 1; Electronic Supplemental Material Appendix 1; Brodo et al. 2001; Knudsen 2007; Breuss 2016; Lendemer et al. 2014, 2017; Tripp and Lendemer 2019a, b, in press).
Conclusions
Results from this study demonstrate clearly that a conspicuous class of obligate symbiotic organisms—lichens—are highly and negatively impacted by disturbance. The present investigation explored patterns not only across a full complement of lichen diversity, but also in a region already characterized by its exceptional overall biodiversity, especially for temperate latitudes. This has important implications for conservation and management, particularly in the southeastern United States—a recognized biodiversity hotspot.
That nearly a third of all lichens in northern Alabama can be considered to be rare in this study (found in few plots) but yet 30 of 47 1 ha plots (64%) contained 50 or more species conveys overall high lichen biodiversity (and high alpha diversity) present in the southernmost portion of southern Appalachian Mountains. These data and the regional species accumulation curve indicate that the region as a whole warrants extensive future study. Optimally, future research should include broader environmental gradients as well as incorporate impacts from biotic factors, especially a clearer understanding of photobiont diversity and distributions. More specifically, it seems likely that biotic and abiotic factors limit different stages of lichen development and, once more fully investigated, this will yield a highly complex and temporally dynamic set of ecological processes that govern lichen biodiversity.
Data accessibility
The entirety of the dataset used herein is included in Electronic Supplemental Material Appendix 1, including the georeferenced locality, elevation, aspect, slope, the number of lichens (total, micro- and macrolichens, sexual and asexual lichens, and four growth forms), disturbances scores (HQ), rock coverage, number of trees, sum of tree diameters, average annual temperature, precipitation, and net primary productivity (NPP) for each of the 47 sites. GIS layers from which the average annual temperature, precipitation, NPP were extracted are publicly available at http://www.worldclim.org/bioclim and http://www.ntsg.umt.edu/project/modis/default.php. OTU tables with taxonomic information, fasta files with representative sequences for each OTU, a metadata file and mapping file are available for download at http://dx.doi.org/10.6084/m9.figshare.1270900. All taxonomic and inventory data are freely available at http://sweetgum.nybg.org/science/vh/.
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Acknowledgements
We are grateful for permission to conduct fieldwork on federal and state lands including the U.S. National Park Service (particularly, Mary Shew, Little River Canyon N.P.), U.S. Forest Service, U.S. Fish and Wildlife Service, and Alabama Department of Conservation and Natural Resources (particularly, Jo Lewis, Chief of Natural Heritage). We thank Carly Anderson Stewart and Jordan Hoffman for contributions to fieldwork and Nolan Kane, Kyle Keepers, Cloe Pogoda, and Kristin White for additional conversation. We additionally are grateful to two anonymous reviewers as well as Anne Pringle whose comments improved an earlier version of the manuscript. This research was made possible by a National Science Foundation Dimensions of Biodiversity Award to University of Colorado (Award #1542629) and New York Botanical Garden (Award #1432629).
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EAT, JCL, and CMM conceived the study, collected the data, conducted the analyses, and wrote the manuscript.
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Tripp, E.A., Lendemer, J.C. & McCain, C.M. Habitat quality and disturbance drive lichen species richness in a temperate biodiversity hotspot. Oecologia 190, 445–457 (2019). https://doi.org/10.1007/s00442-019-04413-0
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DOI: https://doi.org/10.1007/s00442-019-04413-0