Abstract
Plant species of the Chaco seasonally dry forest of central Argentina have presumably been under a low evolutionary pressure to develop specialized fire-response traits, such as heat-stimulated germination. Nevertheless, other historical factors such as seasonal drought and/or endozoochorus dispersal could have led some species to develop heat-tolerant seeds. Therefore, heat-tolerant germination should be more common than heat-stimulated or heat-sensitive germination. To test this, we exposed seeds of 26 dominant species from the Chaco region to a broad range of experimental heat treatments and incubated them for 30 days at 25 °C and 12 h photoperiod. We then scored the percent germination and classified them as heat-stimulated, heat-tolerant or heat-sensitive based on their germination following heat treatments relative to control. Seventeen species showed heat-tolerant germination, including all native graminoids. Seven species showed heat-stimulated germination, under the less-intense heat treatments. Only two species showed heat-sensitive germination. Endozoochory had no influence on germination responses. We suggest that, unlike Mediterranean-climate ecosystems, fire was not a major evolutionary force in the development of Chaco forests, and our results provide greater understanding of the potential response of Chaco plant communities in the face of increasingly frequent fires.
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Introduction
Seed germination is a key process in the life cycles of sexually reproducing plants. Germination can occur under a great variety of environmental conditions because species maximize establishment and survival of new sexually produced individuals in different abiotic and biotic contexts (Bewley and Black 1994; Baskin and Baskin 1998a).
Fire has an important influence on the distribution, composition and evolution of the vegetation cover, especially in fire-prone regions such as Mediterranean-climate ecosystems (Trabaud 1980; Whelan 1995; Keeley 1995; Bond and van Wilgen 1996). Consequently, many plant species have evolved seed germination strategies that optimise plant survival after fire, such as the stimulation of germination by smoke, charred wood and high temperatures associated with fire (Keeley et al. 1985; Keeley and Fotheringham 1998). The latter has been the most studied factor, especially in Mediterranean ecosystems (Keeley 1987; Keeley and Bond 1997; Keeley and Baer-Keeley 1999; Hanley and Lamont 2000; Hanley et al. 2001; Luna et al. 2007; Ne’eman et al. 2009), where some species can tolerate temperatures above 120 °C, or increase germination following the heat shock produced by fire (Keeley 1991; Herranz et al. 1998; Valbuena and Vera 2002; Luna et al. 2007). Other strategies, such as the physical dormancy of seeds (sensu Baskin and Baskin 1998a), due to the impermeability of the seed coat, are also common in these ecosystems. Fire can break seed dormancy by scarifying the seed coat and allowing water and gas exchange (Thanos and Georghiou 1988).
In regions where fire is not as frequent and/or intense as in Mediterranean ecosystems, such as in the South American Chaco, there is very limited knowledge of the effect of heat shock on seed dormancy and germination. Studies have mostly focused on variation of ambient temperatures, ranging from 5 to 35 °C (e.g., Funes and Venier 2006; Funes et al. 2009; Zalazar et al. 2009; Venier 2011).
Given the large influence of climate on plant recruitment mainly through temperature and water supply (Adler and Hill Ris Lambers 2008), climatic alterations and other environmental changes (e.g., land use) predicted for the near future (IPCC 2013) are expected to affect recruitment, through the increase of mean air and soil temperature (Ooi et al. 2012, 2014), and also plant population dynamics in different regions of the world (Arft et al. 1999; Diemer 2002; Fitch et al. 2007; Walck and Dixon 2009; Baeten et al. 2010) including the Chaco forests (Hoekstra et al. 2005; Grau et al. 2008). In addition, increasingly frequent fires due to the expansion of human populations and agricultural lands challenge the adaptive capacity of wild plants to regenerate after fire (Keeley and Fotheringham 2003). Therefore, studies of germination responses to fire are greatly needed for predicting vegetation dynamics under different global change scenarios, and could help elucidate aspects of plant evolution in relation to fire. This question has been widely studied in Mediterranean-climate ecosystems, where correspondences between historical fire regime, regeneration strategy (i.e. resprouting or seeding) and germination in response to heat have been reported for many species (Keeley 1991; Keeley and Bond 1997; Lloret et al. 2005; Luna et al. 2007; Paula and Pausas 2008; Ne’eman et al. 2009).
In this context, it is generally accepted that resprouting ability is an ancestral trait acquired by many lineages in response to different aboveground disturbances, which is widespread both in fire-prone and non-fire-prone ecosystems (Wells 1969; Bellingham et al. 1994; Lloret et al. 1999; Pausas and Verdú 2005). Therefore, resprouting after fire is a less specialized trait; whereas post-fire seed traits, such as the stimulation of seed germination by heat shock, are considered specialised post-fire traits that appeared later in evolution (Wells 1969; Pausas and Verdú 2005; Paula and Pausas 2008).
Several studies report high resprouting capacity of Chacoan species (Tálamo and Caziani 2003; Gurvich et al. 2005; Jaureguiberry 2012; Bravo et al. 2014), and, particularly, Torres et al. (2013) showed that resprouting is more important than seedling recruitment for regeneration after fire in the Chaco Serrano forest, which has some species in common with our study area. This supports the idea that resprouting is one of the main strategies of Chaco species for recovering after fire and other disturbances. However, studies of recruitment from seeds in the Chaco are scarce, hindering the assessment of regeneration strategies after disturbance. For example, Barchuk et al. (1998) reported significant seedling recruitment of Aspidosperma quebracho-blanco in protected areas of the western Chaco, and little recruitment in areas with extensive grazing and uncontrolled logging. Casillo et al. (2012) reported considerable recruitment of graminoids after a fire treatment in a Chaco savanna, whereas forbs were not affected by fire and woody species responded negatively.
Although the Chaco region has been historically subjected to some burning, at least as a result of aboriginal fire (Morello and Saravia Toledo 1959), and also possibly due to occasional lightning during the wet, warm season, there is no record-based evidence of recurring fire having been a strong natural selection pressure over evolutionary time. Therefore, considering both the fire history in the region and the widespread post-fire resprouting ability, it is reasonable to expect that plant species in the Chaco have been subjected to little evolutionary pressure for traits that enhance germination after fire (Paula and Pausas 2008).
However, fire tolerance of seeds of Chaco species could have evolved as a consequence of the evolutionary pressure exerted by other factors, a phenomenon called “exaptation” (Gould and Vrba 1982). Specifically, the presence of hard seed coats, a trait of adaptive value in endozoochorous species dispersed by ungulates (Malo and Suárez 1998), or subjected to seasonal desiccation (Baskin et al. 2000)—both common in the Chaco region—could be an exaptation conferring the seeds a high tolerance to heat. Consequently, heat-tolerant germination rather than heat-stimulated or heat-sensitive germination should be more common.
Here, we test the hypothesis that Western Chacoan species have been subjected to low evolutionary pressure to acquire traits that enhance germination after fire, and other factors with long history in the system could have promoted heat tolerance. To do this, we subjected seeds of the dominant forest species to different experimental heat shock treatments (i.e. different temperature and time of exposure). By comparing the percentages of germination after the heat treatments with those under the control treatments, we aimed to characterize the germination response of the selected species in terms of their sensitivity to heat. This allowed us determine the frequency of each germination response (i.e. heat-sensitive, -tolerant or -stimulated).
Materials and methods
Study area
The study was carried out in an area located in western Córdoba Province, central Argentina (31°17′–31°55′S, 65°12′-65°32′W). Phytogeographically, this corresponds to the Western Chaco (Cabrera 1976), which is characterized by highly seasonal semi-deciduous xerophytic forest. Mean annual precipitation is 450 mm, concentrated in the spring–summer months (October–March). Minimum and maximum mean annual temperatures are 10.7 °C (July) and 26.1 °C (January), respectively. In the dry season (April–October), the water balance is negative, resulting in soil moisture deficit (Cabido et al. 1992, 1994). The forest canopy is c. 12 m high and discontinuous, dominated by Aspidosperma quebracho-blanco and Prosopis flexuosa. The shrub layer (4 m high) is thorny, dense and usually continuous, dominated by Larrea divaricata, Mimozyganthus carinatus and Acacia gilliesii. A lower and discontinuous layer of grasses and herbs is dominated by Trichloris crinita, Gouinia paraguayensis, Setaria spp. and Pappophorum spp. (Cabido et al. 1993).
Plant species selection and seed collection
On the basis of previous surveys (Cabido et al. 1992, Díaz et al. 1998; Conti and Díaz 2013), we selected a total of 26 species that dominated the canopy and understorey of the forest in the study area. They included 11 taxonomic families and different growth forms (8 trees, 10 shrubs, 7 perennial grasses and 1 perennial forb; Table 1). All the species in this study are able to resprout after fire (Jaureguiberry 2012).
With the exception of the exotic graminoid Cenchrus ciliaris, all the selected species are native to the Chaco region (Flora del Cono Sur 2014). Some of them have a wide distribution and can be found both in the western semiarid Chaco subregion (i.e. our study area) as well as in the eastern subhumid Chaco subregion, namely the tree species A. quebracho-blanco, Celtis ehrenbergiana, Castela cocinea and Geoffroea decorticans, the shrubs Acacia aroma, A. caven, A. praecox, Aloysis gratissima and Moya spinosa, and the graminoids Aristida mendocina, G. paraguayensis, Setaria pampeana, Pappophorum vaginatum and Trichloris spp. Other species are more restricted to the semiarid subregion, such as the tree species Cercidium praecox, Prosopis chilensis, P. flexuosa and P. torquata, the shrubs Acacia gilliesii, Condalia microphylla, L. divaricata, M. carinatus and Tricomaria usillo, the forb Justicia squarrosa, and the graminoids C. ciliaris and Neobouteloua lophostachya (Flora del Cono Sur 2014).
Most fire events across all the Chaco region are anthropogenic and are mainly associated with the use of fire as a tool to open agricultural land and to prevent accidental fires (Morello and Saravia Toledo 1959; Morello 1970; PPMF 2007; PNMF 2012). Therefore, it is reasonable to assume that all the species studied have been under a similar selective pressure in terms of fire history.
Seeds were collected from at least 10 healthy-looking, sexually mature, randomly selected individuals of each species, following the sampling protocols of Pérez Harguindeguy et al. (2013), and were then stored in paper bags until the experiments. In some cases, it was necessary to keep the seeds in a freezer (−6 °C) to prevent predation by insects. Post-dispersal units were used for the experiment; therefore, before the experiment seeds were cleaned by removing fruit tissues that would normally be lost in dispersal. In some cases, the “seed” that was incubated included fruit tissues (e.g., endocarp in drupes).
Experimental procedures
The treatments levels used were: control (no heating), 70 °C for 1 h, and 100, 120 and 180 °C for 5 min in a Marne® NEO-LINE digital controlled oven, except T. usillo, subjected only to control and 70 °C treatments, and Castela coccinea, not subjected to the 100 °C treatment due to the limited number of seeds available. We selected these treatments to represent a range of conditions potentially encountered by seeds in the soil at open sites or during fire and within the range of temperatures shown in prior studies to stimulate germination in some species (Keeley 1987; Bóo et al. 1996; Hanley et al. 2001; Luna et al. 2007; Ne’eman et al. 2009; Casillo et al. 2012; Bravo et al. 2014). In a pilot test, we evaluated the response of seeds of three of the selected species (Prosopis flexuosa, Acacia caven and Condalia microphylla) to extreme temperatures of 250, 350 and 450 °C during 3 min in an Indef Model 331 laboratory oven. None of them survived; therefore these temperatures were not included in the subsequent experiment.
After the heat treatments, we placed seeds on filter paper (65 g m−2) within polystyrene dishes, and incubated them in germination chambers at a constant temperature of 25 °C, with a 12/12 h daily photoperiod. Each dish contained 30 seeds of trees and shrubs and 50 seeds of smaller-seeded grasses and forbs. We used three replicates per treatment per species. At the beginning of the experiment, we added 2.5 ml of distilled water per dish with a broad-spectrum fungicide to prevent fungal infection. Seeds were subsequently kept moist by adding distilled water when necessary. The number of germinated seeds was recorded every 2–4 days for 30 days. Germination percentage was defined on the basis of the number of germinated seeds (emerged radicle) per replicate.
Considering previous studies reporting or suggesting the presence of physical dormancy in six of the studied species (Campos et al. 2008; Funes and Venier 2006; Funes et al. 2009; Venier 2011; Venier et al. 2012), the remaining ungerminated seeds of Acacia aroma, Acacia caven, Cercidium praecox, Prosopis chilensis, Prosopis flexuosa and Prosopis torquata were scarified using an 80-grit sandpaper (or a razor blade in smaller seeds) and incubated for 10 additional days. By comparing the germination of dormant seeds after heat treatments alone with the germination after mechanical scarification, we were able to determine the ability of heat shock treatments to break dormancy. To assess germination responses (see below) of seeds with documented or presumed physical dormancy, we considered the germination percentages obtained before mechanical scarification (i.e. those after heat treatment alone).
On the basis of Luna et al. (2007) and Paula and Pausas (2008), we defined three types of germination response in the face of heat shocks:
-
(a)
Heat-sensitive: germination after all the heat treatments significantly lower than the control treatment.
-
(b)
Heat-tolerant: germination after at least one of the heat treatments as high as, but not higher than, the control treatment.
-
(c)
Heat-stimulated: germination after at least one of the heat treatments significantly higher than the control treatment.
Data analysis
All statistical analyses were performed using InfoStat v.2013p (Di Rienzo et al. 2013, Grupo InfoStat, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, Argentina). To determine differences in germination among species and treatments within each species, we analyzed data through a generalized linear model (Bates et al. 2014), which included species, heat treatment and their interaction as fixed factors, assuming a binomial distribution of the error.
To test the influence of the different heat treatments and the presence/absence of endozoochoral dispersal on germination, we used a generalized linear mixed model (Bates et al. 2014). The model included endozoochory, heat treatment and their interaction as fixed factors, and species as random factor. The model was fit using a binomial distribution of the error. Presumed but uncertain presence or absence of endozoochory in some species (referred to as “yes?” or “no?” in Table 1) were considered as “yes” and “no”, respectively, in statistical analyses. The analyses where repeated considering the opposite options, and results did not differ from those reported here.
In all cases, means were compared with the a posteriori DGC test (Di Rienzo, Guzmán, Casanoves pair-wise comparison procedure; Di Rienzo et al. 2002). The model selection was based on the Akaike criterion (1972). The adjustment of the models was made using the software InfoStat (Di Rienzo et al. 2013) which uses lme4 library (Bates et al. 2014) of the R-package (R Development Core Team 2008).
Given the small proportion of seeds that germinated after the 180 °C treatment, we excluded it from the statistical analyses. Nevertheless, results of this treatment are included in tables and figures and discussed in the text.
Results
Germination of species under different heat shock treatments
The percentages of germination recorded under control conditions and after different heat treatments were highly variable between species (Table S1; Fig. S1). Six species showed germination percentages below 20 % after all treatments: Aspidosperma quebracho-blanco, Aloysia gratissima, Larrea divaricata, Condalia microphylla, Geoffroea decorticans and Setaria pampeana. In the three latter germination was <10 %.
Only four out of the 26 species were able to germinate after the 180 °C treatment (Table S1; Fig. S1), from which only Prosopis flexuosa showed a considerable germination percentage (29 %), whereas Cenchrus ciliaris, Neobouteloua lophostachya and Prosopis torquata showed very low germination compared to other treatments (12.7, 4.0 and 1.1 %, respectively). Acacia caven and A. aroma were among the species with the highest germination percentages after all treatments (excluding 180 °C), ranging from 84.4 to 98.9 %. Other species with high germination percentages were A. praecox (71.1–94.4 %), A. gilliesii (60.0–88.9 %), C. ciliaris (71.3–91.3 %) and Aristida mendocina (63.3–76 %).
Characterization of species by germination response categories
Most species (17 out of 26) showed heat-tolerant germination (Fig. 1; Table 1), whereas seven species were stimulated by heat shock treatments: A. gilliesii, Celtis ehrenbergiana, C. ciliaris, Cercidium praecox, Moya spinosa, P. flexuosa and Trichloris spp. Four of these species had their maximum germination after the 70 °C treatment, one after the 100 °C treatment and P. flexuosa and C. praecox after the 120 °C treatment (Table 1). Apart from these two latter species, only two other species had maximum germination at 120 °C (although not significantly different from the control), Justicia squarrosa and Mimozyganthus carinatus (33.3 and 61.1 %, respectively; Table S1). On the other hand, Castela coccinea and Tricomaria usillo were the only species that fell into the heat-sensitive category. Nevertheless, both of them showed low germination percentages in all treatments (Table S1; Fig. S1).
Endozoochory had a significant effect on germination responses only in the 100 °C treatment, for which germination of endozoochorous species was lower than non-endozoochorous ones (Fig. 2).
Effects of heat shock and mechanical scarification on the physical dormancy of seeds
Heat shock alone was effective in breaking physical seed dormancy only in two out of the six species with dormant seeds in this study, namely C. praecox and P. flexuosa (Fig. 1; species d and f, respectively). In both cases, the 120 °C treatment produced a significant increase in germination. Acacia caven showed a slight increase in germination at 70, 100 and 120 °C with respect to control, although this increase was not significant (Fig. 1; species i). A. aroma and P. torquata did not increase their germination in response to heat treatment alone (Fig. 1; species h and w).
With the exceptions of P. flexuosa and P. torquata, mechanical scarification promoted seed germination considerably in four of the six scarified species (Table S1; AS column). The strongest scarification effects were recorded in A. aroma and A. caven. Other species significantly stimulated by scarification were C. praecox and P. chilensis, although both of them showed considerable germination under the control treatment before scarification (14.5 and 36.7 %, respectively; Table S1).
Discussion
Germination responses to heat treatments and mechanical scarification
Percentages of gemination were highly variable. For example, germination of A. caven and A. aroma (both with dormant seeds) after mechanical scarification, and of A. gilliesii (non-dormant seeds) agree with previous studies of their germination requirements (Funes and Venier 2006; Venier et al. 2012). Prosopis chilensis and P. flexuosa have also been reported as having coat-imposed seed dormancy (Cony and Trione 1996), although only P. chilensis responded significantly to scarification in the present study.
The low germination percentages recorded in some other woody species are also consistent with previous studies. For example, Geoffroea decorticans and Condalia microphylla showed percentages of germination similar to those reported in control treatments (at 30/20 °C light/dark) by Renison et al. (2010). Campos et al. (2008) reported very low percentages of germination of Prosopis torquata and P. flexuosa in control treatments. This could be due to overall low seed viability or to specific germination requirements. For example, Renison et al. (2010) reported a significant increase of germination of G. decorticans after the passage of seeds through the digestive tract of frugivorous bird Rhea americana, while Campos et al. (2008) reported a similar pattern for Prosopis species after endozoochoral dispersion by mammals. Other plant species may present physiological mechanisms which inhibit germination (“physiological dormancy”; sensu Baskin and Baskin 2004), which require previous treatments that were not relevant to the main goal of this study, such as thermal stratification (cold or warm) or treatments with gibberellic acid (Baskin and Baskin 2004; Figueroa and Jaksic 2004).
Although some Fabaceae species were stored at cold temperatures before the experiments, and this may affect germination, the fact that temperatures several degrees below zero are not unusual in the study area, together with the very low water content of seeds (which decreases the probability of damage) and the similar germination percentages reported in other studies with seeds of some of the same species but stored at room temperature (e.g., Funes and Venier 2006; Funes et al. 2009; Venier et al. 2012), suggest that cold storage had little or no effect on the germination of these species.
Among the graminoids, Setaria pampeana and Gouinia paraguayensis showed very low germination percentages under all treatments. This could be due to the presence of physiological dormancy, which is common in the Poaceae (Baskin and Baskin 1998b). Evidence indicates that grass seeds usually have week physiological dormancy that can be broken during dry storage at room temperature (Nikolaeva 1969, 1977). This could explain the high percentage of germination of graminoids that are known to have non-deep physiological dormancy such as C. ciliaris (Baskin and Baskin 1998b), while the low germination of S. pampeana and G. paraguayensis could presumably be due to either a deeper level of physiological dormancy, which cannot be broken by dry storage alone, or to low seed viability.
Most of the native graminoids showed heat-tolerant germination. Trichloris spp was the only native heat-stimulated species, although it was under the less intense treatment of 70 °C, while the exotic species C. ciliaris not only showed heat-stimulated germination but also that it was able to germinate after the 180 °C treatment. This is consistent with other studies reporting high fire tolerance in this species. For example, Butler and Fairfax (2003) reported that positive interaction with fire accelerates invasion of this graminoid in Acacia shrublands in northeastern Australia. A similar process has been reported in northern Mexico (Alejandro Castellanos, personal communication)
Heat-tolerant germination and evolutionary history of fire
Our results showed that the seeds of most of species are able to tolerate heat shocks up to 120 °C, but only four survived 180 °C, with few germinated seeds. Most of the species classified as heat-stimulated increased their germination in the 70 °C treatment, which can be considered a moderate or low temperature for a fire. Similar temperatures may be reached in summer in the absence of fire in open sites with high sunshine exposure, such as bare soil patches generated by overgrazing and erosion, or in burned soils after fire (Auld and Bradstock 1996; Pugnaire and Lozano 1997; Keeley and Fotheringham 2000). This suggests that heat stimulation in these species does not necessarily need to be linked to fire. That is to say that both heat-stimulated and heat-tolerant species seem to have a high resistance to heat, but they do not depend on it to germinate (Ooi et al. 2014). Our results also suggest that, although a high germination response could be expected after a low intensity fire in some species, in the presence of high intensity fires, with temperature at soil surface can exceed 600 °C (Bóo et al. 1996)—and therefore temperature at 2 cm deep can reach 200 °C (Bradstock and Auld 1995)—germination can be severely reduced. In this context, the soil insolation capacity, together with the characteristics of the seeds that determine how deep they can bury in soil, play a fundamental role in determining the temperatures at which seeds will be exposed during a fire event. Further studies relating these two factors may help elucidate seed survival probabilities after a fire event.
Most Chaco species that showed high germination at temperatures higher than 70 °C also showed high germination in the control (e.g., several graminoid species; Table S1). This, together with the fact that heat shock alone was able to break seed dormancy only in two of the six dormant-seeded species, supports the hypothesis that species have been under low selective pressure over evolutionary time to develop fire-dependent germination, at least in comparison with other regions of the world. However, many species were tolerant to heat shock, which is the first requirement to recruit after a fire. Consequently, we cannot rule out the possibility of increased post-fire recruitment induced by other fire cues such as smoke, charred wood and/or nitrates. This has been widely studied in species of Mediterranean-climate regions, some of which are capable of germinating only after being exposed to one or more fire-related cues (Keeley 1987; Thanos and Rundel 1995; Keeley and Bond 1997; Keeley and Fotheringham 1998, 2000). Moreover, in the two species in which heat shock was able to break seed dormancy (i.e. Cercidium praecox and Prosopis chilensis), subsequent mechanical scarification produced further increase in germination. This indicates that a significant number of seeds did not respond to heat shock alone.
The high tolerance to heat shock observed in most species suggests that they could have developed exaptations as a consequence of factors other than fire. Among these factors, there is the presence of fruit-eating animals (Traveset 1998; Malo and Suárez 1998). It is reasonable to assume that some species could have developed exaptations to fire as a result of the long history of herbivory by vertebrates in the Chaco region (Bucher 1987; Díaz et al. 1999, 2007). Endozoochory is common in Chacoan species, including several considered in this study, and the traits which originally confer them the capacity to tolerate or take advantage of frugivory could indirectly enhance heat shock tolerance. This appears to be the case of the endozoochorus Celtis ehrenbergiana and Prosopis flexuosa, which were stimulated by heat shock. However, statistical analyses considering all the species only showed a significant influence of endozoochory on germination after the 100 °C treatment, and this result was inconsistent with our hypothesis (i.e. that endozoochorous species would show higher germination). We suggest that high tolerance to heat shock among the studied species could be associated with other factors.
Considering the marked seasonal drought typical of the Chaco region, water stress is one of the most probable factors explaining the thick seed coats of some species, as has been suggested for several families including Fabaceae (Noodén et al. 1985; Baskin et al. 2000). Drought stress during seed development influences seed coat characteristics and germination (Clua et al. 2006). Genetic and/or environmental factors such as soil moisture content (Barton 1965), relative humidity (Harrington 1949; Quinlivan 1971), temperature alternation (Wurzberger and Koller 1976), daily average temperature or thermo-period (von Abrams and Hands 1956) and photoperiodic regime (Koller 1962; Evenari et al. 1966; Karssen 1970; Gutterman and Evenari 1972; Gutterman and Heydecker 1973; Kigel et al. 1977, 1979) during seed development and maturation have been reported to affect seed production and hard-seededness. Therefore, although only few species in this study have hard coats, others may have developed seed coats with characteristics that allow them to tolerate heat and that are induced by drought stress and associated factors.
Conclusions
We show that seed germination of the vast majority of the species studied tolerate low and medium temperature heat-shock treatments, suggesting that they are able to survive moderate intensity fires. Only a few species showed sensitive or stimulated germination by heat shock, and the latter mostly by relatively low temperatures. These results support the hypothesis that fire has been a weak evolutionary force in Chaco forests as compared to Mediterranean-climate ecosystems. We propose that the heat-shock tolerance observed in the seeds of the majority of species is possibly an exaptation to fire, originated in response to other selective factors.
References
Adler PB, Hille Ris Lambers J (2008) The influence of climate and species composition on the population dynamics of ten prairie forbs. Ecology 89:3049–3060
Akaike (1972) Use of an information theoretic quantity for statistical model identification. Proc 5th Hawaii Int Conf Syst Sci. North Hollywood, USA, pp 249–250
Arft AM, Walker MD, Gurevitch J et al (1999) Response patterns of tundra plant species to experimental warming: a meta-analysis of the international tundra experiment. Ecol Monogr 69:491–511
Auld TD, Bradstock RA (1996) Soil temperatures after the passage of a fire: do they influence the germination of buried seeds? Aust J Ecol 21:106–109
Baeten L, De Frenne P, Verheyen K, Graae BJ, Hermy M (2010) Forest herbs in the face of global change: a single-species-multiple-threats approach for Anemone nemorosa. Plant Ecol Evol 143:19–30
Barchuk AH, Díaz MP, Casanoves F, Balzarin MG, Karlin UO (1998) Experimental study on survival rates in two arboreal species from the argentinean dry Chaco. For Ecol Manag 103:203–210
Barton LV (1965) Dormancy in seeds imposed by the seed coat. In: Rhuland W (ed) Encyclopedia of plant physiology, vol 15/2. Springer, Berlin, pp 727–745
Baskin CC, Baskin JM (1998a) Seeds. Ecology, biogeography and evolution of dormancy and germination. Academic, San Diego
Baskin CC, Baskin JM (1998b) Ecology of seed dormancy and germination in grasses. In: Cheplick GP (ed) Population biology of grasses. Cambridge University Press, Cambridge
Baskin JM, Baskin CC, Li X (2000) Taxonomy, anatomy and evolution of physical dormancy in seeds. Plant Spec Biol 15:139–152
Baskin JM, Baskin CC (2004) A classification system for seed dormancy. Seed Sci Res 14:1–16
Bates D, Maechler M, Bolker B, Walker S (2014) lme4: Linear mixed-effects models using Eigen and S4. R package version 1.1-6. http://CRAN.R-project.org/package=lme4
Bellingham PJ, Tanner EVJ, Healey JR (1994) Sprouting of trees in Jamaican Montane forests, after a hurricane. J Ecol 82:747–758
Bewley JD, Black M (1994) Seeds: physiology of development and germination. Plenum, New York
Bond WJ, van Wilgen BW (1996) Fire and plants. Chapman and Hall, London
Bóo RM, Peláez DV, Bunting SC, Elía OR, Mayor MD (1996) Effect of fire on grasses in central semi-arid Argentina. J Arid Environ 32:259–269
Bradstock RA, Auld TD (1995) Soil temperatures during experimental bush fires in relation to fire intensity: consequences for legume germination and fire management in south-eastern Australia. J Appl Ecol 32:76–84
Bravo S, Kunst C, Leiva M, Ledesma R (2014) Response of hardwood tree regeneration to surface fires, western Chaco region, Argentina. For Ecol Manag 326:36–45
Bucher EH (1987) Herbivory in arid and semi-arid regions of Argentina. Rev Chil Hist Nat 60:265–273
Butler DW, Fairfax RJ (2003) Buffel grass and fire in a Gidgee and Brigalow woodland: a case study from central Queensland. Ecol Manag Restor 4:120–125
Cabido M, Acosta A, Carranza ML, Díaz S (1992) La vegetación del Chaco Árido en el W de la provincia de Córdoba, Argentina. Doc phytosociol XIV:447–456
Cabido M, González C, Acosta A, Díaz S (1993) Vegetation changes along a precipitation gradient in central Argentina. Vegetatio 109:5–14
Cabido M, Manzur A, Carranza L, González Albarracín C (1994) La vegetación y el medio físico del Chaco Árido en la provicncia de Córdoba, Argentina Central. Phytocoenologia 24:423–460
Cabrera AL (1976) Regiones Fitogeográficas Argentinas. Enciclopedia Argentina de Agricultura y Jardinería Tomo II. Acme, Buenos Aires
Campos CM, Ojeda RA (1997) Dispersal and germination of Prosopis flexuosa (Fabaceae) seeds by desert mammals in Argentina. J Arid Environ 35:707–714
Campos CM, Peco B, Campos VE, Malo JE, Giannoni SM, Suárez F (2008) Endozoochory by native and exotic herbivores in dry areas: consequences for germination and survival of Prosopis seeds. Seed Sci Res 18:91–100
Casillo J, Kunst C, Semmartin M (2012) Effects of fire and water availability on the emergence and recruitment of grasses, forbs and woody species in a semiarid Chaco savanna. Aust Ecol 37:452–459
Clua A, Fernández G, Ferro L, Dietrich M (2006) Drought stress conditions during seed development of narrowleaf birdsfoot trefoil (Lotus glaber) influences seed production and subsequent dormancy and germination. Lotus Newsl 36:58–63
Conti G, Díaz S (2013) Plant functional diversity and carbon storage—an empirical test in semiarid forest ecosystems. J Ecol 101:18–28
Cony MA, Trione SO (1996) Germination with respect to temperature of two Argentinian Prosopis species. J Arid Environ 33:225–236
De Noir FA, Bravo S, Abdala R (2002) Mecanismos de dispersión de algunas especies leñosas nativas del Chaco Occidental y Serrano. Quebracho 9:140–150
Di Rienzo JA, Guzmán AW, Casanoves F (2002) A multiple-comparisons method based on the distribution of the root node distance of a binary tree. J Agric Biol Envir S 7:129–142
Di Rienzo JA, Casanoves F, Balzarini MG, Gonzalez L, Tablada M, Robledo CW, InfoStat versión (2013) Grupo InfoStat, FCA, Universidad Nacional de Córdoba, Argentina. http://www.infostat.com.ar
Díaz S, Cabido M, Casanoves F (1998) Plant functional traits and environmental filters at a regional scale. J Veg Sci 9:113–122
Díaz S, Cabido M, Zak M, Martínez Carretero E, Araníbar J (1999) Plant functional traits, ecosystem structure and land-use history along a climatic gradient in central-western Argentina. J Veg Sci 10:651–660
Díaz S et al (2007) Plant trait responses to grazing—a global synthesis. Glob Chang Biol 13:313–341
Diemer M (2002) Population stasis in a high-elevation herbaceous plant under moderate climate warming. Basic Appl Ecol 3:77–83
Evenari M, Koller D, Gutterman Y (1966) Effects of the environment of the mother plants on germination by control of seed-coat permeability to water in Ononis sicula Guss. Aust J Biol Sci 19:1007–1016
Figueroa JA, Jaksic FM (2004) Latencia y banco de semillas en plantas de la región mediterránea de Chile central. Rev Chil Hist Nat 77:201–215
Fitch EA, Walck JL, Hidayati SN (2007) Agroecosystem management for rare species of Paysonia (Brassicaceae): integrating their seed ecology and life cycle with cropping regimens in a changing climate. Am J Bot 94:102–110
Flora del Cono Sur (2014) Instituto de Botánica Darwinion-IBODA-CONICET-ANCEFN. [Cited Aug 27th 2014] (http://www2.darwin.edu.ar/Proyectos/FloraArgentina/FA.asp)
Fuentes ER, Hoffmann AJ, Poiani A, Alliende MC (1986) Vegetation change in large clearings: patterns in the Chilean matorral. Oecologia 68:358–366
Funes G, Venier P (2006) Dormancy and germination in three Acacia (Fabaceae) species from central Argentina. Seed Sci Res 16:77–82
Funes G, Díaz S, Venier P (2009) La temperatura como principal determinante de la germinación en especies del Chaco seco de Argentina. Ecol Austral 19:129–138
Gould SJ, Vrba ES (1982) Exaptation—a missing term in the science of form. Paleobiology 8:4–15
Grau HR, Gasparri NI, Aide TM (2008) Balancing food production and nature conservation in the Neotropical dry forests of northern Argentina. Glob Change Biol 14:985–997
Gurvich D, Enrico L, Cingolani AM (2005) Linking plant functional traits with post-fire sprouting vigour in woody species in central Argentina. Aust Ecol 30:789–796
Gutiérrez JR, Armesto J (1981) El rol del ganado en la dispersión de semillas de Acacia caven. Cienc Investig Agrar 8:3–8
Gutterman Y, Evenari M (1972) The influence of day length on seed coat colour, an index of water permeability, of the desert annual Ononis sicula Guss. J Ecol 60:713–719
Gutterman Y, Heydecker W (1973) Studies of the surfaces of desert plant seeds. I. Effect of day length upon maturation of the seed-coat of Ononis sicula Guss. Ann Bot 37:1049–1050
Hanley ME, Lamont BB (2000) Heat pre-treatment and the germination of soil- and canopy-stored seeds of south-western Australian species. Acta Oecol 21:315–321
Hanley ME, Fenner M, Ne’eman G (2001) Pregermination heat shock and seedling growth of fire-following Fabaceae from four Mediterranean-climate regions. Acta Oecol 22:315–320
Harrington JF (1949) Hard seeds in bean and other legumes. Seed World 64:42–44
Herranz JM, Ferrandis P, Martínez-Sánchez JJ (1998) Influence of heat on seed germination of seven Mediterranean Leguminosae species. Plant Ecol 136:95–103
Hoekstra JM, Boucher TM, Ricketts TH, Roberts C (2005) Confronting a biome crisis: global disparities of habitat loss and protection. Ecol Lett 8:23–29
IPCC (2013) Climate Change 2013: the physical science basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, Cambridge
Jaureguiberry P (2012) Caracteres funcionales, flamabilidad y respuesta al fuego de especies vegetales dominantes en distintas situaciones de uso de la tierra en el centro-oeste de Argentina. PhD dissertation, Facultad de Cs. Ex. Fís. y Nat., Universidad Nacional de Córdoba, Córdoba, Argentina
Karssen CM (1970) Light-promoted germination of the seeds of Chenopodium album L. III. Effect of the photoperiod during growth and development of the plants on the dormancy of the produced seeds. Acta Bot Neer 19:81–94
Keeley JE (1987) Role of Fire in Seed Germination of Woody Taxa in California Chaparral. Ecology 68:434–443
Keeley JE (1991) Seed germination and life history syndromes in the California Chaparral. Bot Rev 57:81–116
Keeley JE (1995) Seed-germination patterns in fire-prone Mediterranean-climate regions. In: Arroyo MTK, Zedler PH, Fox MD (eds) Ecology and biogeography of Mediterranean ecosystems in Chile, California, and Australia. Springer, New York, pp 239–273
Keeley JE, Baer-Keeley M (1999) Role of charred wood, heat-shock, and light in germination of postfire Phrygana species from the eastern Mediterranean Basin. Israel J Plant Sci 47:11–16
Keeley JE, Bond WJ (1997) Convergent seed germination in South African fynbos and Californian chaparral. Plant Ecol 133:153–167
Keeley JE, Fotheringham CJ (1998) Smoke-induced seed germination in California Chaparral. Ecology 79:2320–2336
Keeley JE, Fotheringham CJ (2000) Role of fire in regeneration from seed. In: Fenner M (ed) Seeds: the ecology of regeneration in plant communities. CAB International, Wallingford
Keeley JE, Fotheringham CJ (2003) Impact of past, present, and future fire regimes on North American mediterranean shrublands. In: Veblen TT, Baker WL, Montenegro G, Swetnam TW (eds) Fire and climatic change in temperate ecosystems of the Western Americas. Springer, New York
Keeley JE, Morton BA, Pedrosa A, Trotter P (1985) Role of allelopathy, heat and charred wood in the germination of Chaparral herbs and suffrutescents. J Ecol 73:445–458
Kigel J, Offir M, Koller D (1977) Control of the germination responses of Amaranthus retroflexus L. seeds by their parental photothermal environment. J Exp Bot 28:1125–1136
Kigel J, Gibly A, Negbi M (1979) Seed germination in Amaranthus retroflexus L. as affected by the photoperiod and age during flower reduction of the parent plant. J Exp Bot 30:997–1002
Koller D (1962) Preconditioning of germination in lettuce at time of fruit ripening. Am J Bot 49:41–844
Lloret F, Verdú M, Flores-Hernández N, Valiente-Banuet A (1999) Fire and resprouting in Mediterranean ecosystems: insights from an external biogeographical region, the Mexical shrubland. Am J Bot 86:1655–1661
Lloret F, Estevan H, Vayreda J, Terradas J (2005) Fire regenerative syndromes of forest woody species across fire and climatic gradients. Oecologia 146:461–468
Luna B, Moreno JM, Cruz A, Fernández-González F (2007) Heat-shock and seed germination of a group of Mediterranean plant species growing in a burned area: an approach based on plant functional types. Environ Exp Bot 60:324–333
Malo JE, Suárez F (1998) The dispersal of a dry-fruited shrub by red deer in a Mediterranean ecosystem. Ecography 21:204–211
Morello J (1970) Modelos de relaciones entre pastizales y leñosas colonizadoras en el Chaco argentino. IDIA 276:31–52
Morello J, Saravia Toledo C (1959) El Bosque Chaqueño I. Paisaje primitivo, paisaje natural y paisaje cultural en el oriente de Salta. Rev agron noroeste argent III:5–79
Ne’eman G, Ne’eman R, Keith DA, Whelan RJ (2009) Does post-fire plant regeneration mode affect the germination response to fire-related cues? Oecologia 159:483–492
Nikolaeva MG (1969) Physiology of deep dormancy in seeds. National Science Foundation, Washington, DC
Nikolaeva MG (1977) Factors controlling the seed dormancy pattern. In: Khan AA (ed) The physiology and biochemistry of seed development, dormancy and germination. Elsevier, Amsterdam
Noodén LD, Blakley KA, Grzybowski JM (1985) Control of seed coat thickness and permeability in soybean. Plant Physiol 79:543–545
Núñez MB, Bozzolo L (2006) Descripción de la dieta del zorro gris, Pseudalopex griseus (Canidae) (Gray, 1869), en el Parque Nacional Sierra de las Quijadas, San Luis, Argentina. Gayana 70:163–167
Ooi MKJ, Auld TD, Denham AJ (2012) Projected soil temperature increase and seed dormancy response along an altitudinal gradient: implications for seed bank persistence under climate change. Plant Soil 353:289–303
Ooi MKJ, Denham AJ, Santana VM, Auld TD (2014) Temperature thresholds of physically dormant seeds and plant functional response to fire: variation among species and relative impact of climate change Ecol Evol 4:656–671
Paula S, Pausas JG (2008) Burning seeds: germination response to heat treatments in relation to resprouting ability. J Ecol 96:543–552
Pausas JG, Verdú M (2005) Plant persistence traits in fire-prone ecosystems of the Mediterranean basin: a phylogenetic approach. Oikos 109:196–202
Pérez Harguindeguy N, Díaz S, Garnier E, Lavorel S, Poorter H, Jaureguiberry P, Bret-Harte MS, Cornwell WK, Craine JM, Gurvich DE, Urcelay C, Veneklaas EJ, Reich PB, Poorter L, Wright IJ, Ray P, Enrico L, Pausas JG, de Vos AC, Buchmann N, Funes G, Quétier F, Hodgson JG, Thompson K, Morgan HD, ter Steege H, van der Heijden MGA, Sack L, Blonder B, Poschlod P, Vaieretti MV, Conti G, Staver AC, Aquino S, Cornelissen JHC (2013) New handbook for standardised measurement of plant functional traits worldwide. Aust J Bot 61:167–234
Plan Nacional de Manejo del Fuego (PNMF) (2012) Estadística de incendios forestales 2012. Jefatura de Gabinete de Ministros, Presidencia de la Nación, Secretaría de Ambiente y Desarrollo Sustentable de la Nación, Buenos Aires, Argentina. [Cited Aug 27th 2014]. http://www.ambiente.gov.ar/archivos/web/PNEF/file/Incendios%202012%20corregido/Documento%20Completo.pdf
Plan Provincial de Manejo del Fuego (PPMF) (2007) Guía Para La Prevención De Incendios Forestales En Córdoba. Gobierno de la Provincia de Córdoba, Secretaría de Ambiente, Córdoba, Argentina. [Cited Aug 27th 2014]. http://www.cba.gov.ar/wp-content/4p96humuzp/2012/06/Guia-para-la-Pevencion-de-Incendios-Forestales.pdf
Pugnaire F, Lozano J (1997) Effects of soil disturbance, fire and litter accumulation on the establishment of Cistus clusii seedlings. Plant Ecol 131:207–213
Quinlivan BJ (1971) Seed coat impermeability in legumes. J Aust I Agr Sci 37:283–295
R Development Core Team (2008) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, http://www.R-project.org
Renison D, Valladares G, Martella MB (2010) The effect of passage through the gut of the Greater Rhea (Rhea americana) on germination of tree seeds: implications for forest restoration. Emu 110:125–131
Serbent MP, Periago ME, Leynaud GC (2011) Mazama gouazoubira (Cervidae) diet during the dry season in the arid Chaco of Córdoba (Argentina). J Arid Environ 75:87–90
Tálamo A, Caziani SM (2003) Variation in woody vegetation among sites with different disturbance histories in the Argentine Chaco. For Ecol Manag 184:79–92
Thanos CA, Rundel PW (1995) Fire-followers in chaparral: nitrogenous compounds trigger seed germination. J Ecol 83:207–216
Thanos CA, Georghiou K (1988) Ecophysiology of fire-stimulated seed germination in Cistus incanus ssp. creticus (L.) Hey wood and C. salvifolius L. Plant, Cell Environ 11:841–849
Torres R, Giorgis M, Trillo C, Volkmann L, Demaio P, Heredia J, Renison D (2013) Post-fire recovery occurs overwhelmingly by resprouting in the Chaco Serrano forest of Central Argentina. Austral Ecol 39:346–354
Trabaud L (1980) Impact biologique et écologique des feux de végétation sur l’organisation, la structure et l’évolution de la végétation des garrigues du Bas-Languedoc. PhD thesis, Université Montpellier li Sciences et Techniques du Languedoc, Montpellier
Traveset A (1998) Effect of seed passage through vertebrate frugivores’ guts on germination: a review. Persp Plant Ecol Evol Syst 1(2):151–190
Valbuena L, Vera ML (2002) The effects of thermal scarification and seed storage on germination of four heathland species. Plant Ecol 161:137–144
Varela O, Bucher EH (2006) Passage time, viability, and germination of seeds ingested by foxes. J Arid Environ 67:566–578
Venier P (2011) ¿Pueden los caracteres regenerativos explicar la coexistencia de especies de Acacia (Fabaceae) en los bosques xerófilos de la Provincia de Córdoba? PhD dissertation, Universidad Nacional de Córdoba, Córdoba
Venier P, Funes G, Carrizo García C (2012) Physical dormancy and histological features of seeds of five Acacia species (Fabaceae) from xerophytic forests in central Argentina. Flora 207:39–46
Von Abrams GJ, Hand ME (1956) Seed dormancy in Rosa as a function of climate. Am J Bot 43:7–12
Walck JL, Dixon KW (2009) Time to future-proof plants in storage. Nature 462:721
Wells PV (1969) The relation between mode of reproduction and extent of speciation in woody genera of the California chaparral. Evolution 23:264–267
Whelan RJ (1995) The ecology of fire. Cambridge University Press, Cambridge
Wurzberger J, Koller D (1976) Differential effects of the parental photothermal environment on development of dormancy in caryopses of Aegilops kotschyi. J Exp Bot 27:43–48
Zalazar M, Funes G, Venier P (2009) Factores que afectan la germinación de Justicia squarrosa Griseb, forrajera nativa de la región chaqueña de la Argentina. Agriscientia 26:1–6
Zuloaga FO, Morrone O (1996) Catálogo de las Plantas Vasculares de la República Argentina I. Monogr Syst Bot Mo Bot Gard 60:1–323
Zuloaga FO, Morrone O (1999) Catálogo de las Plantas Vasculares de la República Argentina II. Monogr Syst Bot Mo Bot Gard 74:1–1269
Zuloaga FO, Nicora EG, Rúgolo de Agrasar ZE, Morrone O, Pensiero J, Cialdella AM (1994) Catálogo de la Familia Poaceae en la República Argentina. Monogr Syst Bot Mo Bot Gard 47:1–178
Acknowledgments
We are grateful to J. Di Rienzo and F. Casanoves for statistical advice and to G. Funes for useful comments on experimental design. We thank Dr. Juan Armesto and two anonymous reviewers for useful comments that greatly helped improving this paper. This study is a contribution of Núcleo DiverSus, endorsed by DIVERSITAS and the IGBP Global Land Project, and supported by FONCyT, CONICET, SeCyT-Universidad Nacional de Córdoba and the Inter-American Institute for Global Change Research (IAI) CRN 2015 and SGP-CRA 2015, which were supported by the US National Science Foundation (grants GEO-0452325 and GEO-1138881). The experiments comply with the current laws of the country (Argentina) in which the experiments were performed.
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Jaureguiberry, P., Díaz, S. Post-burning regeneration of the Chaco seasonally dry forest: germination response of dominant species to experimental heat shock. Oecologia 177, 689–699 (2015). https://doi.org/10.1007/s00442-014-3161-x
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DOI: https://doi.org/10.1007/s00442-014-3161-x