Abstract
In dark-grown soybean (Glycine max [L.] Merr.) seedlings, exposing the roots to water-deficient vermiculite (ψw=−0.36 MPa) inhibited hypocotyl (stem) elongation. The inhibition was associated with decreased extensibility of the cell walls in the elongation zone. A detailed spatial analysis showed xyloglucan endotransglucosylase (XET; EC 2.4.1.207) activity on the basis of unit cell wall dry weight was decreased in the elongation region after seedlings were transplanted to low ψw. The decrease in XET activity was at least partially due to an accumulation of cell wall mass. Since cell number was only slightly altered, wall mass had increased per cell and probably led to increased wall thickness and decreased cell wall extensibility. Alternatively, an increase in cell wall mass may represent a mechanism for regulating enzyme activity in cell walls, XET in this case, and therefore cell wall extensibility. Hypocotyl elongation was partially recovered after seedlings were grown in low-ψw vermiculate for about 80 h. The partial recovery of hypocotyl elongation was associated with a partial recovery of cell wall extensibility and an enhancement of XET activity in the hypocotyl elongation zone. Our results indicate XTH proteins may play an important role in regulating cell wall extensibility and thus cell elongation in soybean hypocotyls. Our results also showed an imperfect correlation of spatial elongation and XET activity along the hypocotyls. Other potential functions of XTH and their regulation in soybean hypocotyl growth are discussed.
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Introduction
Cell elongation and expansion in plants is a complex but well-coordinated process. During elongation, biochemical processes keep the cell walls extensible and turgor pressure extends the walls irreversibly, making the wall compartment larger (Cleland 1971; Taiz 1984; Cosgrove 1993, 1999). At the same time, the expansion of the walls keeps the turgor pressure from reaching its maximum, resulting in a lower water potential (ψw) in the elongating cells than in the non-elongating cells (Boyer 1968, 1985, 1988, 2001). The lower ψw derived from cell expansion, i.e., the growth-induced ψw, draws water from the source such as the xylem or non-elongating cells and into the elongating cells. Theoretically, anything that can change the turgor or the growth-induced ψw or the extensibility (ability of the wall to be irreversibly extended by turgor pressure) may in turn affect the elongation rate.
Water deficit inhibits cell elongation in plants by affecting turgor, cell wall extensibility or the growth-induced ψw. Turgor pressure was often fully maintained in stem and leaf growing regions at low ψw when elongation was inhibited, indicating that the inhibition of stem and leaf elongation by low ψw was due, at least partially, to a decrease in cell wall extensibility (Michelena and Boyer 1982; Van Volkenburgh and Boyer 1985; Westgate and Boyer 1985; Nonami and Boyer 1990a; Chazen and Neumann 1994; Cramer and Bowman 1991). Root elongation was also inhibited by low ψw but to a lesser extent. A correlation between decreased cell wall extensibility and cell elongation was also reported in roots at low ψw (Pritchard et al. 1993). One study with maize primary roots showed that cell wall extensibility in the very apical region was actually increased at low ψw and maintained cell elongation when turgor pressure was greatly reduced (Spollen and Sharp 1991; Wu et al. 1996). An increase in wall extensibility in roots at low ψw was also suggested in several other studies (Kuzmanoff and Evans 1981; Hsiao and Jing 1987; Itoh et al. 1987; Frensch and Hsiao 1994, 1995; Triboulot et al. 1995; Pritchard et al. 1993).
The biochemical basis of changes in wall extensibility is still not clear. Wall metabolism will affect the components in the wall, and the ratio of different polymers and activity of different wall loosening and tightening agents may play important roles in regulating wall extensibility (Carpita and Gibeaut 1993; Carpita et al. 1996; Cosgrove 1999). Studies with cultured tobacco cells (Iraki et al. 1989a, 1989b), grape leaves (Sweet et al. 1990), wheat coleoptiles (Wakabayashi et al. 1997), cucumber hypocotyls (Sakurai et al. 1987) and roots (Zhong and Lauchli 1993) showed that wall composition was greatly affected by dehydration or exposure to high concentrations of salts or polyethylene glycol. In general, wall material accumulated at lower rates at low ψw, and associated with this reduction was a decrease in [14C]glucose incorporation to less than 50% of the control rate in expanding grape leaf tissue, with the greatest inhibition of cellulose synthesis (Sweet et al. 1990). This was also true in NaCl-treated cotton roots (Zhong and Lauchli 1988). In chick-pea, osmotic stress caused an increase in α-galactosidase, an autolytic activity (Muñoz et al. 1993).
Proteins in cell walls are also thought to be involved in controlling cell wall extensibility under water deficit. Xyloglucan endotransglucosylase/hydrolases (XTHs) are wall proteins that exhibit two distinct enzymatic activities: xyloglucan endotransglucosylase (XET) and/or xyloglucan endohydrolase (XEH) (Rose et al. 2002). Since the XET activity of XTHs can cleave and graft xyloglucan molecules, XTHs may play a role in cell wall loosening and metabolism (Smith and Fry 1991; Nishitani and Tominaga 1991; Fry et al. 1992; Nishitani 1995; Fry 2004). However, XTH failed to cause cucumber cell walls to extend in an in vitro assay (McQueen-Mason et al. 1993). It was believed that XTH might be involved in elongation growth indirectly. A recent study showed that a tomato XTH (tXET) was able to cause creep of cell wall analogues (bio-composite materials based on Acetobacter xylinus cellulose and xyloglucan), supporting the notion that XTH may indeed play important roles in regulating wall mechanical properties and thus the growth process in plants (Chanliaud et al. 2004). Expansins are the only proteins so far capable of causing plant cell wall extension in vitro and are believed to play a critical role in controlling cell wall extensibility and thus cell elongation (McQueen-Mason et al. 1992). In primary roots of maize, dehydration caused an increase in XET activity specifically in the root elongation zone (Wu et al. 1994). In the same region of maize primary roots, the activity and amount of expansin proteins were increased under low ψw (Wu et al. 1996). The enhancement of XET and expansin activities is closely associated with an increase in cell wall extensibility (Wu et al. 1996), suggesting these cell wall proteins are playing important roles in controlling cell wall extensibility and thus elongation of the roots at low ψw.
It is not known how these cell wall proteins are regulated in the stems when cell elongation is inhibited at low ψw. The objective of this study was to examine XET activity in soybean hypocotyls (later to become stems) whose elongation was inhibited by low ψw in association with a reduction in cell wall extensibility.
Materials and methods
Soybean seedling culture and harvest
Soybean (Glycine max [L.] Merr. cv. Williams) seeds were disinfected in 1% NaOCl for 8 min, rinsed in water for 1 h, and germinated in vermiculite in saturating humidity in the dark at 29°C. The vermiculite was hydrated with a 5 mM calcium solution (2.5 mM CaCl2 and 2.5 mM Ca(NO3)2, Ψw of −0.06 MPa). After 48–50 h, seedlings with uniform hypocotyl and root lengths were transplanted into vermiculite pre-adjusted to −0.06 MPa or −0.36 MPa using various amounts of 5 mM Ca2+ solution. Vermiculite Ψw was measured by isopiestic thermocouple psychrometry (Boyer and Knipling 1965). When necessary for experiments, illumination was provided by a green safelight (Nonami and Boyer 1989). This seedling culture method differs slightly from our previous methods (Meyer and Boyer 1972; Cavalieri and Boyer 1982; Nonami et al. 1997) because the vermiculite we could obtain recently adsorbs large amounts of Ca2+, releasing K+ in exchange and causing Ca2+-deficiency symptoms. With 2.5 mM CaCl2 and 2.5 mM Ca(NO3)2, the exchange sites were saturated and sufficient Ca2+ was available in the bulk solution to be equivalent to past experiments and to sustain fast growth for several days.
Hypocotyl elongation was determined from the length increase measured with a ruler at various times. Elongation zones of hypocotyls were determined from the separation of ink marks applied with a paintbrush (Higgins, Eberhard Faber, Lewisburg, TN, USA). Marking with a paintbrush did not inhibit hypocotyl elongation. The space between marks and the diameter of the hypocotyls were determined with a dissecting microscope having a calibrated ocular scale.
Cell wall extensibility measurement
Cell wall extensibility was measured as a relative plastic deformability. The elongation zone of the hypocotyl was first stretched with a constant force (20 g) for 9–12 min to measure total tissue extension. Removal of the force caused a contraction of the tissue (elastic extension). A net plastic extension was calculated by subtracting elastic extension from total tissue extension (Nonami and Boyer 1990a). To calculate relative plastic deformability, the net plastic extension was divided by the length of the elongation zone and then multiplied by the average cross-sectional area of the elongation zone, then divided by the force (20 g). The unit will be equivalent to MPa−1 (Nonami and Boyer 1990a).
Assay for XET activity
In order to analyze the spatial distribution of XET activity, the whole hypocotyls were cut into serial segments (4–20 mm long, depending on the length of the elongating regions). At each serial position, segments were pooled and immediately frozen in liquid nitrogen. Samples were weighed and stored at −76°C until XET activity was assayed.
Hypocotyl (0.3–1.7 g fresh weight, equivalent to 14–16 hypocotyl segments) tissues were homogenized with a mortar and pestle in ice-cold 50 mM Mes (pH 6.0), 10 mM sodium ascorbate, and 10 mM CaCl2. The homogenate was centrifuged for 10 min at 8,800 g and 4°C, and the supernatant was used for the XET assay. The activity was assayed according to Fry et al. (1992) using 0.075% xyloglucan (from Tropaeolum seeds), 5 mM sodium ascorbate, 5 mM CaCl2, 50 mM Mes (pH 6.0), 0.2 μM (400 Bq) of reduced xyloglucan heptasaccharide ([3H]XXXGol; purified from digested Tamarind xyloglucan, labeled at carbon 1 of the glucitol), and 10 μl of extract in a final reaction volume of 40 μl. The assay mixture was incubated at 25°C in a water bath for 1 h, during which the rate of the reaction was linear. During the enzyme reaction, XET transfers unlabeled large xyloglucan molecules to 3H-labeled oligosaccharides ([3H]XXXGol) and the product (labeled large xyloglucan molecules) is adsorbed by hydrogen bonding to the cellulose in the filter paper (Whatman #3 MM). The unreacted oligosaccharide label did not bind and was rinsed away. XET activity was calculated from the counts bound to the filter paper. Background XET activity was determined by deleting xyloglucan from the assay, in which case the assay depended on endogenous xyloglucans in the tissue extract. Subtracting background activity from total activity did not affect the results, and the data are presented for total XET activity.
XET activities were expressed on the basis of total soluble protein or cell wall dry weight. Soluble protein content was measured (Bradford 1976) in the samples used for XET assay but cell wall dry weight was measured in parallel samples because a large amount of tissue was required. Hypocotyl (1.8–4 g fresh weight) tissues were homogenized with mortar and pestle in 1.5% SDS. After centrifuging at 11,000 g for 10 min, the pellets were resuspended and walls were purified by the method of Wu et al. (1994) based on procedures in Fry (2000). This method removes proteins with phenol/glacial acetic acid (5:2) and starch with dimethyl sulfoxide (DMSO; 90%). After cell walls were washed three times with 70% ethanol, the wall pellets were dried at 80°C for 24 h.
In order to calculate the rate of cell wall deposition along the hypocotyls, the relative rate of cell expansion at a particular position was first estimated from the curve fitting the spatial elongation pattern. The rate of cell wall deposition was then calculated by multiplying the estimated relative elongation rate by the cell wall dry weight at each particular position along the hypocotyls.
Cell length measurement
Hypocotyls from the seedlings that had been transplanted into vermiculite having high or low Ψw for 24 h were cut longitudinally using a razor blade. The lengths of the cells in the middle four layers of cortical tissue were measured under the microscope. Three cells were measured at each position per hypocotyl. Three hypocotyls were used for measurement in each experiment of total four experiments.
Results
Elongation growth and cell wall extensibility
After transplanting to vermiculite at high ψw, hypocotyls continued to grow rapidly (Fig. 1a). Hypocotyl elongation was about 2 mm h−1 right after transplanting and accelerated to a rate of 3 mm h−1 before dropping sharply when the hypocotyl above the cotyledons (epicotyl) emerged at about 60–70 h (Fig. 1b). Low ψw caused an immediate near cessation of hypocotyl elongation for 25 h (Fig. 1a). The hypocotyls elongated at about 10% of the rate at high ψw initially, then more rapidly until they reached about 50% of the maximum rate at high ψw (Fig. 1b). The elongating region became longer as the hypocotyls lengthened (Fig. 1c). The lengthening of the hypocotyl elongation zone was delayed by low ψw. The delay was caused mostly by the decreased rate of hypocotyl growth because at the same hypocotyl length the elongation zone had a similar length regardless of ψw (Fig. 1c, inset).
Associated with the inhibition of hypocotyl elongation at low ψw was a decrease in cell wall extensibility. Relative plastic deformability was constant with time at high ψw. Low ψw caused a 35% reduction in cell wall extensibility. When hypocotyl elongation was partially recovered after 80 h in low ψw, cell wall extensibility was also partially recovered, showing only 19% reduction (Table 1).
Hypocotyl elongation and cell wall extensibility were reported in our previous studies (Nonami and Boyer 1990a). Due to the change in growth medium, we re-examined these parameters. The results showed little difference from our previous studies.
Spatial distribution of XET activity along the hypocotyl
To study if XET activity was associated with the decrease in cell wall extensibility, we examined the spatial distribution of XET activity along the hypocotyls. XET activity (on a total soluble protein and unit length basis) was unaffected or even increased in certain regions of the hypocotyl elongation zone after 48 h at low ψw compared to high ψw (Fig. 2a,b). On the basis of unit cell wall dry weight, however, XET activity was greatly reduced at low ψw in the growing zone of the hypocotyl (Fig. 2c). Beyond the 20-mm region of rapid elongation, XET activity remained high and extended into the mature region. Only in the most mature basal part of the hypocotyl did the activity begin to decline. When hypocotyl elongation had partially recovered after 96 h at low ψw, XET activity had more than fully recovered in the elongation zone on the basis of cell wall dry weight or unit length (Fig. 2a,c). The activity peaked in the non-elongating region between the 20- and 60-mm positions 96 h after low-ψw treatment regardless of the basis of expression.
Changes in cell wall dry weights
The discrepancy in XET activity between per unit cell wall dry weight and per unit length indicated an accumulation of cell wall mass in hypocotyls at low ψw. Cell wall dry weights were uniformly greater along the hypocotyls after 48 h at low ψw (Fig. 2e). The increases were apparent by 24 h (data not shown). When hypocotyl elongation returned to an intermediate rate in the seedlings exposed to low ψw (96 h, Fig. 1b, 2d), wall dry weights became the same as in the high-ψw control at 48 h (Fig. 2e). Multiplying the relative rate of elongation (Fig. 2d) by the wall dry weight (Fig. 2e) gave the deposition rates for new wall dry weight, i.e., the rate of wall biosynthesis at various positions along the hypocotyl (Fig. 2f). In the hypocotyls, biosynthesis was diminished after 48 h at low ψw and remained somewhat less after 96 h compared to the high-ψw control (Fig. 2f).
In order to determine whether the increased dry weight of the cell walls was caused by an increase in cell number at low ψw (the cells would be shorter), cell length was measured along the hypocotyls in the first 25 mm at 24 h after transplanting. Cell length in the first 15 mm was the same at high and low ψw (Fig. 3). The first 15 mm encompassed nearly the whole elongation region at low ψw, but the final cell length was shorter at low ψw than at high ψw (Fig. 3) because low ψw caused an earlier cessation of cell expansion.
In order to address the possibility that other forms of cell expansion might occur outside of the elongation zone of the hypocotyls and roots, we examined the spatial distribution of radial expansion. However, little radial expansion occurred outside of the zone of elongation in the hypocotyl (Fig. 4). Hypocotyl diameters averaged 2 mm (Fig. 4 inset).
Discussion
XTHs have been proposed to be important for regulating the polymer length and insertion of xyloglucans into the cell wall, which could alter the extensibility of the cell wall (Nishitani and Tominaga 1991; Smith and Fry 1991; Thompson and Fry 2001; Fry et al. 1992). The decrease in XET activity in soybean hypocotyls on the basis of cell wall dry weight and low extensibility after 48 h at low ψw supports this concept, and the surge in XET activity and partial recovery in extensibility by 96 h in the hypocotyls adds further evidence favoring the concept. A decrease in XET activity was also reported in the basal 5–10 mm of maize primary roots treated with polyethylene glycol solution (ψw=−0.96 MPa) for 24 h, which was correlated with a decrease in cell wall extensibility and cell elongation in that region (Pritchard et al. 1993). In the apical 5–6 mm of maize primary roots at low ψw (−1.6 MPa), however, Wu et al. (1994) found an enhanced XET activity, which was correlated with an increase in cell wall extensibility (Wu et al. 1996). These results strongly support a potential role of XTH associated with cell wall extensibility and thus cell elongation.
Low ψw induced by osmoticum typically allows wall synthesis to continue (Bonner 1934; Loescher and Nevins 1972; Iraki et al. 1989a; Wakabayashi et al. 1997; Sakurai et al. 1987; Zhong and Lauchli 1993). In soils having low ψw, Sweet et al. (1990) found continued wall synthesis but at a lower rate than at high ψw, consistent with our results. In soybean hypocotyls, rates of elongation were even more affected than the rate of cell wall synthesis. As a result, despite the lower rates of biosynthesis, the wall dry weight increased. Because the overall effect on cell number (4% in 25 mm) was too small to account for the increased wall dry weight in the hypocotyls at low ψw, i.e. the dimensions were unchanged in most of the cells of the elongating region, the accumulation of cell wall dry weight led to more wall per cell, suggesting that greater wall mass might have thickened the walls. Bret-Harte et al. (1991) found thicker walls in tissues whose growth had been inhibited by osmoticum. The increase in cell wall thickness in soybean hypocotyl at low ψw may cause a decrease in cell wall extensibility directly or/and through a dilution of wall-loosening proteins in cell walls, i.e. a decrease in XET activity in unit of cell wall mass.
Under conditions similar to ours, Nonami and Boyer (1990a, 1990b) found low extensibility in hypocotyls of soybean seedlings grown for 24–48 h at low ψw. In the present work (96 h), the extensibility recovered to an intermediate level afterward, when wall synthesis recovered slightly. The wall mass returned to control levels after this recovery. Therefore, a correlation existed at low ψw between wall extensibility, biosynthesis, and dry mass. The lower rates of synthesis may have contributed to the decreased extensibility of the walls in the hypocotyl tissues. It also is possible that the composition of the walls changed and might have contributed to the loss in extensibility. Iraki et al. (1989b) and Sweet et al. (1990) found less cellulose forming in walls at low ψw than at high ψw, and Iraki et al. (1989a) showed that the cellulose-deficient walls had a lower tensile strength. This supports the concept that cellulose reinforces the walls against the bursting tendencies of turgor pressure, but Iraki et al. (1989b) point out that extensibility probably depends more on the hemicelluloses than celluloses.
Unlike roots, whose spatial distribution of XET activity is closely correlated with cell elongation (Pritchard et al. 1993; Wu et al. 1994), the highest XET activity in the soybean hypocotyls is located beyond the elongation zone (Fig. 2a–c). A similar pattern was found in asparagus spears with the highest XET activity at the base where elongation had ceased (O’Donoghue et al. 2001). Since radial expansion of the soybean hypocotyl stops as cell elongation ceases at the end of the elongation zone, the highest XET activity may be associated with other processes in the hypocotyls. XTH is comprised of a multigene family (de Silva et al. 1994; Okamoto et al. 1994; Schünmann et al. 1997; Campbell and Braam 1999; Uozu et al. 2000) associated with many physiological processes (reviewed by de Silva et al. 1994; Nishitani 1995, 1998; Campbell and Braam 1999). We may have detected activity of different XTH isozymes along the length of the hypocotyls. Different XTH isozymes may exist in the elongation zone and non-elongation zone, which may explain why the total XET activity imperfectly matches the spatial cell elongation in the hypocotyls. In support of this notion, Catalá et al. (2001) reported that two tomato XTH genes, LeEXT and LeEXT2 were differentially expressed in the etiolated hypocotyls. LeEXT was abundantly expressed in the rapid elongation region, while LeXET2 was expressed more abundantly in the mature/non-elongating region. In soybean, there are at least four XTHs that have been studied. They are involved in different processes, such as cell expansion and response to elicitor from bacteria (Okazawa et al. 1993; Zurek and Clouse 1994; Hagihara et al. 2004). Additional ESTs for soybean XTH can be found in the TIGR soybean gene database (http://www.tigr.org).
It needs to be pointed out that XET activity assayed in vitro may not necessarily represent the action in vivo (Fry 2004). In maize primary roots, a higher expansin activity was found in the region near maturation of cell elongation (basal 5 mm) than in the region of fastest elongation (apical 5 mm; Wu et al. 1996). However, the basal 5 mm of the roots only showed very limited capability of responding to expansins in an in vitro reconstitution assay, indicating that factors other than expansins are controlling cell wall extensibility in the basal region. One of the possible explanations is that the modification of cell wall structure in the basal region of the roots makes cell walls less susceptible to expansin action (Wu et al. 1996). This could be applied to the XET activity in the soybean hypocotyl also, i.e. a higher XET activity in the non-elongating region assayed in vitro may not function in vivo due to limited access to its substrates. XET activity in vivo will also depend on the type of substrate. A recent study indicated that XTH might be capable of promoting or suppressing cell elongation, depending on the substrates used. Integration of long xyloglucan molecules into pea hypocotyl without epidermis could suppress cell elongation, but addition of xyloglucan oligosaccharide promoted cell elongation of pea hypocotyl (Takeda et al. 2002). Thus, caution must be taken when interpreting any results based on total XET activity from an in vitro assay without knowing in vivo substrates.
It is interesting that the amount of cell wall mass accumulated in the hypocotyl elongation zone during early low-ψw treatment (48 h) disappeared after hypocotyl elongation had partially recovered (Fig. 2a–e). The enhancement of XET activity in the first 40 mm of the soybean hypocotyl at 96 h after low-ψw treatment is closely correlated with the disappearance of the accumulated cell wall mass. Although the disappearance could have been caused by the displacement of the growing cells into the mature region as new cells were added to the elongating region, the cells in the mature region showed little evidence of greater wall dry weight. Therefore, it is likely that wall dry weight was mobilized by the enlarging cells during their recovery to intermediate rates of growth. One of the processes that XTH is known to be involved in is seed xyloglucan mobilization (de Silva et al. 1993; Fanutti et al. 1993). It also is possible that some XTHs in the soybean hypocotyls are performing a similar function and are involved in mobilization of xyloglucan at low ψw.
In summary, our studies demonstrated a close correlation between change in XET activity and cell wall extensibility. Both the increase in cell wall thickness and a decrease in XET activity in cell walls may contribute to the decrease in cell wall extensibility and thus inhibition of cell elongation in soybean hypocotyls at an early stage of low-ψw treatment. Our studies may also indicate other functions of XTH in soybean hypocotyl growth.
Abbreviations
- ψ w :
-
Water potential (MPa)
- XET :
-
Xyloglucan endotransglucosylase
- XTH :
-
Xyloglucan endotransglucosylase/hydrolase
- XXXGol :
-
Reduced xyloglucan heptasaccharide
References
Bonner J (1934) Studies on the growth hormone of plants. V. The relation of cell elongation to cell wall formation. Proc Natl Acad Sci USA 20:393–397
Boyer JS (1968) Relationship of water potential to growth of leaves. Plant Physiol 43:1056–1062
Boyer JS (1985) Water transport. Annu Rev Plant Physiol 36:473–516
Boyer JS (1988) Cell enlargement and growth-induced water potentials. Physiol Plant 73:311–316
Boyer JS (2001) Growth-induced water potentials originate from wall yielding during growth. J Exp Bot 52:1483–1488
Boyer JS, Knipling EB (1965) Isopiestic technique for measuring leaf water potentials with a thermocouple psychrometer. Proc Natl Acad Sci USA 54:1044–1051
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 72:248–254
Bret-Harte MS, Baskin TI, Green PB (1991) Auxin stimulates both deposition and breakdown of material in the pea outer epidermal cell wall, as measured interferometrically. Planta 185:462–471
Campbell P, Braam J (1999) Xyloglucan endotransglycosylases: diversity of genes, enzymes and potential wall modifying functions. Trends Plant Sci 4:361–366
Carpita NC, Gibeaut DM (1993) Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. Plant J 3:1–30
Carpita NC, McCann M, Griffing LR (1996) The plant extracellular matrix: news from the cell’s frontier. Plant Cell 8:1451–1463
Catalá C, Rose JKC, York WS, Albersheim P, Darvill AG, Bennett AB (2001) Characterization of a tomato xyloglucan endotransglycosylase gene that is down-regulated by auxin in etiolated hypocotyls. Plant Physiol 127:1180–1192
Cavalieri AJ, Boyer JS (1982) Water potentials induced by growth in soybean hypocotyls. Plant Physiol 69:492–496
Chanliaud E, de Silva J, Strongitharm B, Jeronimidis G, Gidley MJ (2004) Mechanical effects of plant cell wall enzymes on cellulose/xyloglucan composites. Plant J 38:27–37
Chazen O, Neumann PM (1994) Hydraulic signals from the roots and rapid cell-wall hardening in growing maize (Zea mays L.) leaves are primary responses to polyethylene glycol-induced water deficits. Plant Physiol 104:1385–1392
Cleland RE (1971) Cell wall extension. Annu Rev Plant Physiol 22:197–222
Cosgrove DJ (1993) Wall extensibility: its nature, measurement, and relationship to plant cell growth. New Phytol 124:1–23
Cosgrove DJ (1999) Enzymes and other agents that enhance cell wall extensibility. Annu Rev Plant Physiol Plant Mol Biol 50:391–417
Cramer GR, Bowman DC (1991) Kinetics of maize leaf elongation. I. Increased yield threshold limits short-term, steady-state elongation rates after exposure to salinity. J Exp Bot 42:1417–1426
de Silva J, Jarman CD, Arrowsmith DA, Stronach MS, Chengappa S, Sidebottom C, Reid JSG (1993) Molecular characterization of a xyloglucan-specific endo-(1–4)-β-d-glucanase (xyloglucan endo-transglycosylase) from nasturtium seeds. Plant J 3:701–711
de Silva J, Arrowsmith D, Hellyer A, Whiteman S, Robinson S (1994) Xyloglucan endotransglycosylase and plant growth. J Exp Bot 45:1693–1701
Fanutti C, Gidley MJ, Reid JSG (1993) Action of a pure xyloglucan endo-transglycosylase (formerly called xyloglucan-specific endo-(1–4)-β-d-glucanase) from the cotyledons of germinated nasturtium seeds. Plant J 3:691–700
Frensch J, Hsiao TC (1994) Transient responses of cell turgor and growth of maize roots as affected by changes in water potential. Plant Physiol 104:247–254
Frensch J, Hsiao TC (1995) Rapid response of the yield threshold and turgor regulation during adjustment of root growth to water stress in Zea mays. Plant Physiol 108:303–312
Fry SC (2000) The growing plant cell wall: chemical and metabolic analyses. Reprint Edition, Blackburn Press, Caldwell, NJ.
Fry SC (2004) Tansley Review: Primary cell wall metabolism: tracking the careers of cell wall polymers in living plant cells. New Phytol 161:641–675
Fry SC, Smith RC, Renwick KF, Martin DJ, Hodge SK, Matthews KJ (1992) Xyloglucan endotransglycosylase, a new wall-loosening enzyme activity from plants. Biochem J 282:821–828
Hagihara T, Hashi M, Takeuchi Y, Yamaoka N (2004) Cloning of soybean genes induced during hypersensitive cell death caused by syringolide elicitor. Planta 218:604–614
Hsiao TC, Jing J (1987) Leaf and root expansive growth in response to water deficits. In: Cosgrove DJ, Knievel DP (eds) Physiology of cell expansion during plant growth. American Society of Plant Physiologists, Rockville, MD, pp 180–192
Iraki NM, Bressan RA, Hasegawa PM, Carpita NC (1989a) Alteration of the physical and chemical structure of the primary cell wall of growth-limited plant cells adapted to osmotic stress. Plant Physiol 91:39–47
Iraki NM, Singh N, Bressan RA, Carpita NC (1989b) Cell wall of tobacco cells and changes in composition associated with reduced growth upon adaptation to water and saline stress. Plant Physiol 91:48–53
Itoh K, Nakamura Y, Kawata H, Ohta E, Sakata M (1987) Effect of osmotic stress on turgor pressure in mung bean root cells. Plant Cell Physiol 28:978–994
Kuzmanoff KM, Evans ML (1981) Kinetics of adaptation to osmotic stress in lentil (Lens culinaris Med.) roots. Plant Physiol 68:224–247
Loescher W, Nevins DJ (1972) Auxin induced changes in Avena cell wall composition. Plant Physiol 50:556–563
McQueen-Mason, SJ, Durachko DM, Cosgrove DJ (1992) Two endogenous proteins that induce cell wall extension in plants. Plant Cell 4:1425–1433
McQueen-Mason SJ, Fry SC, Durachko DM, Cosgrove DJ (1993) The relations between Xyloglucan endotransglycosylase and in vitro wall extension in cucumber hypocotyls. Planta 190:327–331
Meyer RF, Boyer JS (1972) Sensitivity of cell division and cell elongation to low water potentials in soybean hypocotyls. Planta 108:77–87
Michelena VA, Boyer JS (1982) Complete turgor maintenance at low water potentials in the elongating region of maize leaves. Plant Physiol 69:1145–1149
Muñoz FJ, Labrador E, Dopico B (1993) Effect of osmotic stress on the growth of epicotyls of Cicer arietinum in relation to changes in the autolytic process and glycanhydrolytic cell wall enzymes. Physiol Plant 87:544–551
Nishitani K (1995) Endo-xyloglucan transglycosylase, a new class of transferase involved in cell wall construction. J Plant Res 108:137–148
Nishitani K (1998) Construction and restructuring of the cellulose–xyloglucan framework in the apoplast as mediated by the xyloglucan-related protein family—a hypothetical scheme. J Plant Res 111:1–8
Nishitani K, Tominaga R (1991) In vitro molecular weight increase in xyloglucans by an apoplastic enzyme preparation from epicotyls of Vigna angularis. Physiol Plant 83:490–497
Nonami H, Boyer JS (1989) Turgor and growth at low water potentials. Plant Physiol 89:798–804
Nonami H, Boyer JS (1990a) Wall extensibility and cell hydraulic conductivity decrease in enlarging stem tissues at low water potentials. Plant Physiol 93:1610–1619
Nonami H, Boyer JS (1990b) Primary events regulating stem growth at low water potentials. Plant Physiol 93:1601–1609
Nonami H, Wu Y, Boyer JS (1997) Decreased growth-induced water potential: a primary cause of growth inhibition at low water potentials. Plant Physiol 114:501–509
O’Donoghue EM, Somerfield SD, Sinclair BK, Coupe SA (2001) Xyloglucan endotransglycosylase: a role after growth cessation in harvested asparagus. Aust J Plant Physiol 28:349–361
Okamoto S, Kamimai T, Nishitani K (1994) Molecular analyses of the gene in Arabidopsis thaliana which encodes endo-xyloglucan transferase. In: Abstracts of the 4th international congress of plant molecular biology, Amsterdam. No. 586
Okazawa K, Sato Y, Nakagawa T, Asada K, Kato I, Tomita E, Nishitani K (1993) Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls. J Biol Chem 268:25364–25368
Pritchard J, Hetherington PR, Fry SC, Tomos AD (1993) Xyloglucan endotransglycosylase activity, microfibril orientation and the profiles of cell wall properties along growing regions of maize roots. J Exp Bot 44:1281–1289
Rose JKC, Braam J, Fry SC, Nishitani K (2002) The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature. Plant Cell Physiol 43:1421–1435
Sakurai N, Tanaka S, Kuraishi S (1987) Changes in wall polysaccharides of squash (Cucurbita maxima Duch.) hypocotyls under water stress condition. I. Wall sugar composition and growth as affected by water stress. Plant Cell Physiol 28:1051–1058
Schünmann PHD, Smith RC, Lang V, Matthews PR, Chandler PM (1997) Expression of XET-related genes and its relation to elongation in leaves of barley (Hordeum vulgare L.). Plant Cell Environ 20:1439–1450
Smith RC, Fry SC (1991) Endotransglycosylation of xyloglucans in plant-cell suspension-cultures. Biochem J 279:529–535
Spollen WG, Sharp RE (1991) Spatial distribution of turgor and root growth at low water potentials. Plant Physiol 96:438–443
Sweet WJ, Morrison JC, Labavitch JM, Matthews MA (1990) Altered synthesis and composition of cell wall of grape (Vitis vinifera L.) leaves during expansion and growth-inhibiting water deficits. Plant Cell Physiol 31:407–414
Taiz L (1984) Plant cell expansion: regulation of cell wall mechanical properties. Annu Rev Plant Physiol 35:587–657
Takeda T, Furuta Y, Awano T, Mizuno K, Mitsuishi Y, Hayashi T (2002) Suppression and acceleration of cell elongation by integration of xyloglucans in pea stem segments. Proc Natl Acad Sci USA 99:9055–9060
Thompson JE, Fry SC (2001) Restructuring of wall-bound xyloglucan by transglycosylation in living plant cells. Plant J 26:23–34
Triboulot MB, Pritchard J, Tomos AD (1995) Stimulation and inhibition of pine root growth by osmotic stress. New Phytol 130:169–175
Uozu S, Tanaka-Ueguchi M, Kitano H, Hattori K, Matsuoka M (2000) Characterization of XET-related genes of rice. Plant Physiol 122:853–859
Van Volkenburgh E, Boyer JS (1985) Inhibitory effects of water deficit on maize leaf elongation. Plant Physiol 77:190–194
Wakabayashi K, Hoson T, Kamisaka S (1997) Change in amounts and molecular mass distribution of cell-wall polysaccharides of wheat (Titicum aestivum L.) coleoptiles under water stress. J Plant Physiol 151:33–40
Westgate ME, Boyer JS (1985) Osmotic adjustment and the inhibition of leaf, root, stem, and silk growth at low water potentials in maize. Planta 164:540–549
Wu Y, Spollen WG, Sharp RE, Hetherington PR, Fry SC (1994) Root growth maintenance at low water potentials. Increased activity of xyloglucan endotransglycosylase and its possible regulation by abscisic acid. Plant Physiol 106:607–615
Wu Y, Sharp RE, Durachko SM, Cosgrove DJ (1996) Growth maintenance of the maize primary roots in cell-wall extension properties, expansin activity, and wall susceptibility to expansins. Plant Physiol 111:765–772
Zhong H, Lauchli A (1988) Incorporation of [14C]glucose into cell wall polysaccharides of cotton roots: effects of NaCl and CaCl2. Plant Physiol 88:511–514
Zhong H, Lauchli A (1993) Changes of cell wall composition and polymer size in primary roots of cotton seedlings under high salinity. J Exp Bot 44:773–778
Zurek DM, Clouse SD (1994) Molecular cloning and characterization of a brassinosteroid-regulated gene from elongating soybean (Glycine max L.) epicotyls. Plant Physiol 104:161–170
Acknowledgement
We thank Dr. Jennifer MacAdam for her critical comments on this manuscript. This work was supported by DOE Grant-DE-FG02-87ER13776 to J.S.B., and a BBSRC grant to S.C.F., and by the Utah Agricultural Experiment Station to Y.W.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wu, Y., Jeong, BR., Fry, S.C. et al. Change in XET activities, cell wall extensibility and hypocotyl elongation of soybean seedlings at low water potential. Planta 220, 593–601 (2005). https://doi.org/10.1007/s00425-004-1369-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00425-004-1369-4