Abstract
To identify the pathogen that causes scuticociliatosis in farmed-raised turbot Scophthalmus maximus, we isolated a ciliate from the brain tissue of an infected turbot and identified it as Uronema marinum based on morphological and molecular evidence. We then infected the turbots in artificial laboratory settings with pure cultured U. marinum. The infected turbots showed syndromes similar to those observed in naturally infected ones. Furthermore, microscopic examination and PCR detection confirmed the presence of the ciliate in the tissues of those laboratory-infected turbots. To our best knowledge, this is the first report of scuticociliatosis caused by U. marinum in farm-raised turbot S. maximus in China.
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Acknowledgment
The LZLP2013 is morphologically identified by Prof. HU Xiaozhong and Dr. LIU Mingjian from the Laboratory of Protozoa, Ocean University of China.
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6 Data Availability Statement
The 18S rRNA gene sequence of ciliate LZLP2013 is available from the National Center for Biotechnology Information under the accession number MF418591. The COX I gene sequence of ciliate LZLP2013 is available from the National Center for Biotechnology Information under the accession number MG372368.
Supported by the National Key R&D Program of China (No. 2017YFC1404504), the National Basic Research Program of China (973 Program) (No. 2015CB755904), the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology (Nos. 2015ASKJ02, 2016ASKJ14), and the Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology (No. 2016LMFS-B08)
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Du, G., Qu, L., Shang, K. et al. Ciliate Uronema marinum is the causative agent of scuticociliatosis in farm raised turbot Scophthalmus maximus. J. Ocean. Limnol. 37, 1726–1735 (2019). https://doi.org/10.1007/s00343-020-8305-8
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DOI: https://doi.org/10.1007/s00343-020-8305-8