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Erratum to: Diabetologia
DOI 10.1007/s00125-016-3868-9
Problems regarding Fig. 2 have come to light after publication of this paper. Specifically:
Fig. 2a
For cells expressing PI-GFP and p62-mCherry at time point 0 (top row on the right-hand panel) the PI-GFP (green) and p62-mCherry (red) channels were mistakenly reversed in the merged image. This mistake is corrected in the figure shown overleaf and does not affect the colocalisation analysis (Fig. 2b), which was performed on correctly merged images.
In the second row of Fig. 2a the green intensity in the right-most (‘Merge’) panel is higher than that in the PI-GFP single channel image to the left. The authors confirmed that they adjusted the brightness and contrast in the merged image for cosmetic reasons after quantifications were performed. The exposure in the merged image is corrected in the amended figure shown overleaf. Colocalisation analyses were performed using identical imaging conditions for the control and different treatments so these changes do not affect the interpretation of the findings. The figure legend has been amended to reflect this.
Modest changes in brightness and contrast were uniformly applied to the images in the amended figure to reduce non-specific background fluorescence, which was not organelle-specific. Unmodified images were used for quantifications. The legend has been amended in line with this.
Fig. 2c
In the bottom row of Fig. 2c, a technical problem meant that the images were cropped differently and were not properly aligned in size, shape or with respect to one another. This is corrected overleaf. To avoid any misinterpretation the images are shown using identical brightness and contrast conditions for all single-channel and merged images.
The p62-mCherry (red) signal in the bottom middle panel of Fig. 2c was selectively enhanced from the original image, contravening the journal’s image manipulation policy (http://www.diabetologia-journal.org/instructionstoauthors.html#manipulation). The authors have recreated Fig. 2c from scratch. Slight adjustments to intensity and contrast were applied uniformly to the single and merged images; the colour balance remained untouched. All quantifications were performed prior to representative image modifications.
Modest changes in brightness and contrast were uniformly applied to the images in the amended figure to reduce non-specific background fluorescence, which was not organelle-specific, and unmodified images were used for quantifications. The legend has been amended in line with this.
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The online version of the original article can be found at http://dx.doi.org/10.1007/s00125-016-3868-9.
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Riahi, Y., Wikstrom, J.D., Bachar-Wikstrom, E. et al. Erratum to: Autophagy is a major regulator of beta cell insulin homeostasis. Diabetologia 59, 1575–1576 (2016). https://doi.org/10.1007/s00125-016-3986-4
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DOI: https://doi.org/10.1007/s00125-016-3986-4