Abstract
In the parasitic waspNasonia vitripennis, certain males carry a B chromosome, called PSR (paternal sex ratio), which causes the compaction and subsequent loss of the paternal chromosomes in fertilized eggs. BecauseNasonia are haplo-diploid, this leads to the production of all-male broods. Three families (PSR2, PSR18, PSR22) of related, tandemly repetitive DNAs were shown to be present solely on the PSR chromosome. These three families shared two conserved, palindromic ANA sequences, which may play a role in either PSR function or amplification of the tandem arrays. The tandem repeat family NV79 was determined to be present on the PSR chromosome as well as on at least one of the A chromosomes. This shared repeat as well as two repeat families (NV85, NV126) that were localized on the A chromosomes were detected in two sibling species ofN. vitripennis. NV79 and NV126 were also found in the more distantly related species,Trichomalopsis dubius.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Benton WD, Davis RW (1977) Screening λgt recombinant clones by hybridization to single plaques in situ. Science 196: 180–182
Bigot Y, Hamelin M-H, Periquet G (1990) Heterochromatin condensation and evolution of unique satellite-DNA families in two parasitic wasp species:Diadromus pulchellus andEupelmus vuilleti (Hymenoptera). Mol Biol Evol 7: 351–364
Boucek Z (1988) Australian chalcidoidea [Hymenoptera]. CAB International, Wallingford, UK
Breeuwer JAJ, Werren JH (1990) Microorganisms associated with chromosome destruction and reproductive isolation between two insect species. Nature 346: 558–560
Darling DC, Werren JH (1990) Biosystematics ofNasonia (Hymenoptera: Pteromalidae): Two new species reared from birds' nests in North America. Ann Entomol Soc Am 83: 352–370
Halazonetis TD, Kandil AN (1991) Determination of the c-MYC DNA-binding site. Proc Natl Acad Sci USA 88: 6162–6266
Jones RN, Rees H (1982) B Chromosomes. Academic Press, New York
Kreitman M, Hudson R (1991) Inferring the evolutionary histories of theAdh andAdh-dup loci inDrosophila melanogaster from patterns of polymorphism and divergence. Genetics 127: 565–582
Lam BS, Carroll D (1983) Tandemly repeated DNA sequences fromXenopus laevis I. Studies on sequence organization and variation in satellite 1 DNA (741 base-pair repeat). J Mol Biol 165: 567–585
Lee W, Haslinger A, Karin M, Tjian R (1987) Activation of transcription by two factors that bind promoter and enhancer sequences of human metallothionein gene and SV40. Nature 325: 368–372
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Miklos GLG, Gill AC (1981) The DNA sequences of cloned complex satellite DNA From HawaiianDrosophila and their bearing on satellite DNA sequence conservation. Chromosoma 82: 409–427
Nur U, Werren JH, Eickbush DG, Burke WD, Eickbush TH (1988) A “selfish” B chromosome that enhances its transmission by eliminating the paternal genome. Science 240: 512–514
Ryan SL, SaulII GB, Conner GW (1985) Aberrant segregation of R-locus genes in male progeny from incompatible crosses inMormoniella. J Hered 76: 21–26
Ryan SL, SaulII GB, Conner GW (1987) Separation of factors containing R locus genes inMormoniella stocks derived from aberrant segregation following incompatible crosses. J Hered 78: 273–275
Sanger F, Nicklen S, Coulson A (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Werren JH (1991) The PSR (paternal sex ratio) chromosome. Am Nat 137: 392–402
Werren JH, van denAssem J (1986) Experimental analysis of a paternally inherited extrachromosomal factor. Genetics 114: 217–233
Werren JH, Skinner SW, Charnov EL (1981) Paternal inheritance of a daughterless sex ratio factor. Nature 293: 467–468
Werren JH, Nur U, Eickbush D (1987) An extrachromosomal factor causing loss of paternal chromosomes. Nature 327: 75–76
Author information
Authors and Affiliations
Additional information
by H.F. Willard
Rights and permissions
About this article
Cite this article
Eickbush, D.G., Eickbush, T.H. & Werren, J.H. Molecular characterization of repetitive DNA sequences from a B chromosome. Chromosoma 101, 575–583 (1992). https://doi.org/10.1007/BF00660317
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00660317