Abstract
The influx of phosphate across the mucosal border of different regions of rabbit small intestine was investigated using the technique of Schultz et al., J. Gen. Physiol.50, 1241–1260 (1967). In the duodenum, the phosphate influx consisted of two components: 1. a saturable part, inhibited competitively by the presence of arsenate in the mucosal solution and strongly dependent on the mucosal Na concentration, and 2. a Na-independent part, linearly related to the mucosal phosphate concentration. In the jejunum and the ileum, the phosphate influx was a linear function of the mucosal phosphate concentration. In these regions arsenate had no effect on the influx, supporting the idea of a diffusional transport. HgCl2 (0.5 mM) reduced the phosphate influx in the duodenum, at 140 mM Na, to the levels under Na-free conditions. The Na-independent influx was only slightly decreased by HgCl2, suggesting that this agent affects mainly the Na-dependent phosphate influx. In the ileum HgCl2 decreased the influx by about the same amount as under Na-free conditions in the duodenum. Thus, in the rabbit, the duodenum only appears to have a Na-dependent, carrier mediated phosphate transport mechanism at the mucosal membrane. In the jejunum and the ileum the phosphate uptake seems to be by simple diffusion.
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Danisi, G., Straub, R.W. Unidirectional influx of phosphate across the mucosal membrane of rabbit small intestine. Pflugers Arch. 385, 117–122 (1980). https://doi.org/10.1007/BF00588690
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DOI: https://doi.org/10.1007/BF00588690