Abstract
Background
Scintigraphic image analysis of99mTc-mertiatide (Mag-3, mercaptoacetyltriglycine) clearance provides the determination of the blood flow, the tubular transit time and the excretion as well from both kidneys. Radiopharmaceutical routine recommends a radiochemical purity control before administration of the product to a patient. The main objective of this study is to develop a Mag-3 labeling procedure that fits better than the previous one in our daily routine production of radiopharmaceuticals.
Methods
Increasing proportions of99mTc-Mag-3 were measured during the heating and cooling steps of the Mag-3 labeling procedure. HPLC analysis was used to confirm the results of a rapid radiochemical quality control assay on standard ITLC-SG paper. Results: The reconstitution time takes 20-25 minutes from the harvest of pertechnetate to a ready-for-use calibrated patient syringe. The HPLC profile of99mTc-Mag-3 including its minor impurities remains unchanged for 24-48 hours after reconstitution.
Conclusions
The application of a programmable Peltier-directed device for heating/cooling provides a better control of the temperature course. The procedure proposed fully meets the labeling criteria recommended by the supplier and can be performed with a minimum of attention within a time-span that we formerly needed for solely the radiochemical purity control assay. Moreover,99mTc-Mag-3 prepared in this way seems to be considerably more stable than mentioned in the manufacturer’s instructions.
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References
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van Hemert, F.J., van Lenthe, H., Schimmel, K.J.M. et al. Preparation, radiochemical purity control and stability of99mTc-mertiatide (Mag-3). Ann Nucl Med 19, 345–349 (2005). https://doi.org/10.1007/BF02984631
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DOI: https://doi.org/10.1007/BF02984631