Summary
The preparation of antimacrophage serum (AMS) and its application to murine fibrous histiocytoma are reported. AMS, prepared by inoculating peritoneal macrophages from mice into rabbits, was purified by repeated absorption with kidney homogenate, red blood cells, thymus cells, non-adherent spleen cells and finally, L-cells. The molecular weight was determined as 170,000 daltons using SDS-polyacrylamide gel electrophoresis. AMS diluted to 1:32 reacted with 61.4% of peritoneal macrophages, but not red blood cells, lymphocytes, granulocytes and fibroblasts, as judged by membrane immunofluorescence.
57.1% of monoclonal B-10 macrophages and 75.6% of 28–12 macrophages transformed with SV40 reacted with AMS. B-10 and 28–12 cell tumors simulating human malignant fibrous histiocytoma gave a distinctive diffuse pattern of immunofluorescence with AMS which affected both round- and spindle-shaped cells, although positive fluorescence of spindle-shaped cells was somewhat weaker than that of round-shaped cells. However, tumor tissues from a murine fibrosarcoma showed no reaction with AMS. Based on these findings, AMS may be useful in determining the histogenesis of some soft tissue tumors of doubtful origin.
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Argyris BF, Plotkin DM (1969) Effect of antimacrophage serum on antibody production and phagocytosis in mice. J Immunol 103:372–373
Enjoji M, Hashimoto H, Tsuneyoshi M, Iwasaki H (1980) Malignant fibrous histiocytoma — A clinicopathologic study of 130 cases. Acta Pathol Jpn 30:727–741
Fu Yao-shi, Gabbiani G, Kaye GI, Lattes R (1975) Malignant soft tissue tumors of probable histiocytic origin (malignant fibrous histiocytoma): General considerations and electron microscopic and tissue culture studies. Cancer 35:176–198
Gallily R (1971) In vitro and in vivo studies of the properties and effects of antimacrophage sera (AMS). Clin Exp Immunol 9:381–391
Gallily R, Gornostansky M (1971) Specificity and nature of binding of antimacrophage serum. Immunol 22:431–439
Glenis E, Tiller Eg, McGiven AR, Pihl E (1973) Detection of mouse macrophage antigens by antimacrophage serum. Aust J Exp Biol Med Sci 51:793–800
Hajdu SI (1979) Pathology of soft tissue tumors. Lea & Febiger, Philaderphia, pp 94–121
Hashimoto K, Morimoto K, Akeho M, Yumoto T (1979) Electron microscopic observation of the primary malignant fibrous histiocytoma: Comparison with the giant cell tumor of bone and the giant cell tumor of tendon sheath. J Clin Electron Micro 12:730–731
Hirsch MS, Gary GW, Murphy FA (1969) In vitro and in vivo properties of antimacrophage sera. J Immunol 102:656–661
Kouri J, Ancheta O (1972) Transformation of macrophages into fibroblasts. Exp Cell Res 71:168–176
Morimoto K (1980) Experimental study on fibrous histiocytoma. J Yonago Med Assoc 31:230–253 (in Japanese)
Ozzello L, Stout AP, Murray MR (1963) Cultural characteristics of malignant histiocytomas and fibrous xanthomas. Cancer 16:331–344
Pearson LD, Osebold JW (1973) Effects of antithymocyte and antimacrophage sera on the survival of mice infected with Listeria monocytogenes. Infection and Immunity 7:479–486
Pearson LD, Osebold JW (1974) Effects of antithymocyte sera and antimacrophage sera on cell-mediated immune reactions in Listeria-infected mice. Infection and Immunity 9:127–133
Persson LI, Rönnbäck L (1979) Demonstration of cross reaction between anti-macrophage antibodies and mononuclear mesodermal cells. Experientia 35:381–382
Stinnett JW, Kaplan MA, Morahan SP (1976) Identification of a macrophage-specific cell surface antigen. J Immunol 116:273–278
Takayama H (1980) Transformation of mouse peritoneal macrophages and bone marrow cells by simian virus 40. Microbiol Immunol 24:155–167
Thompson WD, Jack AS, Richmond J, Patrick RS (1982) The mononuclear phagocyte system of the mouse as demonstrated by the immunoperoxidase technique using anti-mouse macrophage antiserum. Diag Histopathol 5:19–32
Unanue ER (1968) Properties and some uses of anti-macrophage antibodies. Nature 218:36–39
Walder JC, Kaplan AM, Mohanakumar T (1980) Human mononuclear phagocyte-associated antigens. I. Identification and characterization. Cell Immunol 59:399–410
Weiss SW, Enzinger FM (1978) Malignant fibrous histiocytoma. An analysis of 200 cases. Cancer 41:2250–2266
Yumoto T, Morimoto K (1980) Experimental approach to fibrous histiocytoma. Acta Pathol Jpn 30:767–778
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Yoshida, H., Yamamoto, S. & Yumoto, T. The identification and characterization of antimacrophage serum (AMS) and its application to murine fibrous histiocytoma. Virchows Archiv B Cell Pathol 43, 127–138 (1983). https://doi.org/10.1007/BF02932950
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DOI: https://doi.org/10.1007/BF02932950