Abstract
Fibronectin is a glycoprotein found in soluble form in plasma and in an insoluble form in connective tissue matrices. Fibronectin isolated from canine peritoneal effusions by affinity chromatography on gelatin-Sepharose was found to be immunologically indistinguishable from the plasma form, as shown by double immunodiffusion techniques. Utilizing an enzyme immunoassay technique, 7 patients with disseminated splenic hemangiosarcoma were found to have peritoneal fibronectin concentrations of 309 ± 133 (mean ± SD) μg/ml. In 8 patients with widespread lymphoma the peritoneal fibronectin concentrations were 323 ± 147 μg/ml. In 5 patients with hepatoma the peritoneal fibronectin concentrations were 361 ± 121 μg/ml. In 6 patients, 2 with mesothelioma, 2 with pancreatic adenocarcinomas, and 2 with prostatic adenocarcinoma, the peritoneal fibronectin concentrations were 315 ± 115 μg/ml. The lowest concentrations 117 ± 46 μg/ml, were found in patients with malignant disease and concurrent peritoneal thrombus formation. These data suggest that decreased fibronectin concentrations in peritoneal effusions are associated with, at least, a locally hypercoagulable state.
Sammendrag
Fibronectin er et glycoprotein, der forekommer i en opløselig form i plasma og i en uopløselig form i bindevævsgrundsubstans. Fibronectin isoleret fra peritoneale effusioner hos hunde ved affinitetskromatografi kunne immunologisk ikke skelnes fra plasmaformen ved anvendelse af immunodiffusion. Med enzymimmunoassay fandtes 7 patienter med dissemineret hæmangiosarkom i muten at have en peritoneal fibronectinkoncentration på 309 ± 133 (gns. ± SD) µg/ml. Hos 8 patienter med udbredt lymfom var koncentrationen 323 ± 147 µg/ml og hos 5 dyr med hepatom 361 ± 121 µg/ml. Hos 6 patienter, hvoraf 2 med mesotheliom, 2 med pancreasadenokarcinom og 2 med prostatakarcinom, var koncentrationen af fibronectin i peritonealvæsken 315 ± 115 µg/ml. De laveste koncentrationer, nemlig 117 ± 46 µg/ml blev fundet hos patienter med neoplasi ledsaget af dannelsen af thromber i mesenteriets arterier. Disse data peger på, at nedsat indhold af fibronectin i peritoneale effusioner er ledsaget af øget koagulation i hvert fald lokalt.
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This project was supported by the Danish Agricultural and Veterinary Research Council, grant No. 13-3682.
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Feldman, B.F., Brummerstedt, E., Larsen, L.S. et al. Fibronectin in Neoplastic Peritoneal Effusions in Dogs. Acta Vet Scand 29, 273–279 (1988). https://doi.org/10.1186/BF03548618
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DOI: https://doi.org/10.1186/BF03548618