Summary
Normal and pathological lymphoid cells, collected from different sources, were cryopreserved using a programmed freezing procedure. With this cryopreservation technique, the percentage of T and B cell surface markers and the proliferative response to mitogens were not influenced by 21 and 35 days of storage in liquid nitrogen. The recovery percentage of the lymphoid cells was satisfactory when fetal calf serum was added, as a protein source, to the medium during freezing and thawing phases, while a very low percentage of cells was recovered if the fetal calf serum was omitted.
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Supported by grant no. 7701469 from theConsiglio Nazionale delle Ricerche (CNR), Rome, Italy andStiftung Volkswagenwerk, Bundesrepublik Deutschland.
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Delfini, C., Grilli, G., Polchi, P. et al. Cryopreservation of human lymphoid cells from various tissues. La Ricerca Clin. Lab. 9, 61–66 (1979). https://doi.org/10.1007/BF02905028
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DOI: https://doi.org/10.1007/BF02905028