Abstract
The production of extracellular polysaccharides from carob pod extract by Aureobasidium pullulans in batch fermentation was investigated. Optimum conditions for polysaccharide productivity, polysaccharide yield, and fermentation efficiency were: initial sugar concentration of 25 g/L, initial pH 6.5, and temperature 25–30°C. A maximum polysaccharide concentration (6.5 g/L), polysaccharide productivity (2.16 g/L/d), total biomass concentration (6.3 g/L), and polysaccharide yield (30%) were obtained with inoculum at 10% (v/v), initial sugars in carob pod extract of 25 g/L, pH 6.5, and 25°C. The highest values of pullulan proportion (70% of total polysaccharides) and fermentation efficiency (89%) were assumed at initial sugar concentration of 25 g/L, pH 6.5 and 30°C. Structural characterization of purified pullulan samples by monosaccharide and methylation analyses, 13C-NMR, and pullulanase digestion combined with size-exclusion chromatography revealed the presence of mainly α-(l → 4) (68%) and α-(l → 6) (31%) glucosidic linkages; however, small amounts (<1%) of triply linked (1, 3, 4-and 1, 4, 6-Glc) glucose residues were detected. The molecularsize distribution and intrinsic viscosity of pullulan derived from culture grown at pH 4.5 (30°C) showed a higher molecular weight than its counterpart obtained at pH 6.5 (30°C).
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Roukas, T., Biliaderis, C.G. Evaluation of carob pod as a substrate for pullulan production byAureobasidium pullulans . Appl Biochem Biotechnol 55, 27–44 (1995). https://doi.org/10.1007/BF02788746
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DOI: https://doi.org/10.1007/BF02788746