Abstract
Certain species of bacteria can become competent to take up high molecular weight DNA from the surrounding medium. DNA homologous to resident chromosomal DNA is transported, processed and recombined with the resident DNA. There are some variations in steps leading to transformation between Gram-positive bacteria likebiplococcus pneumoniae and Gram-negative bacteria represented byHaemophilus influenzae but the integration is by single-strand displacement in both cases. Plasmid (RSF0885) transformation is low inHaemophilus influenzae but this is increased significantly if (homologous) chromosomal DNA is spliced to plasmid DNA. InHaemophilus influenzae, rec1 function is required for peak transformation with chimeric plasmids. Chimeric plasmid fixed presumably extrachromosomally undergoes frequent recombination between homologous segments contained in resident chromosome and the plasmid.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- Mr :
-
Molecular weight
- CF:
-
competence factor
- BF:
-
binding factor
References
Balganesh, M. and Setlow, J. K. (1984)Symp. Plasmids, (In Press).
Barany, F., Kahn, M. and Smith, H. O. (1983)Proc. Natl. Acad. Sci. USA,80, 7274.
Barany, F. and Tomasz, A. (1980)J. Bacteriol.,144, 698.
Biswas, G. D., Sox, T., Blackman, E. and Sparling, P. F. (1977)J. Bacteriol.,129, 983.
Bodmer, W. F. (1965)J. Mol. Biol.,14, 534.
Bodmer, W. F. and Ganesan, A. T. (1964)Genetics,50, 717.
Bott, K. F. and Wilson, G. A. (1968)Bacteriol., Rev.,32, 370.
Cahn, F. H. and Fox, M. S. (1968)J. Bacteriol.,95, 867.
Canosi, U., Morrelli, G. and Trautner, T. A. (1978)Mol. Gen. Genet.,166, 259.
Canosi, U., Iglesias, A. and Trautner, T. A. (1981)Mol. Gen. Genet.,181, 434.
Caster, J. H., Postel, E. H. and Goodgal, S. H. (1970)Nature (London),227, 515.
Chang, S. and Cohen, S.N. (1979)Mol Gen. Genet.,168, 111.
Chilton, M. D. (1967)Science,157, 817.
Chung, B. C. and Goodgal, S. H. (1979)Biochem. Biophys. Res. Commun.,88, 208.
Claverys, J. P., Mejean, V., Gase, A. M. and Sicard, A. M. (1983)Proc. Natl. Acad. Sci. USA,80, 5956.
Concino, M. F. and Goodgal, S. H. (1981)J. Bacteriol.,148, 220.
Concino, M. F. and Goodgal, S. H. (1982)J. Bacteriol.,152, 441.
Danner, D. B., Deich, R. A., Sisco, K. L. and Smith, H. O. (1980)Gene,11, 311.
Danner, D. B., Smith, H. O. and Narang, S. A. (1982)Proc. Natl. Acad. Sci. USA,79, 2392.
Deich, R. A. and Smith, H. O. (1980)Mol. Gen. Genet.,177, 369.
de Vos, W. M., Venema, G., Conosi, U. and Trautner, T. A. (1981)Mol. Gen. Genet.,181, 424.
Eisenstadt, E., Lange, R, and Willecke, K. (1975)Proc. Natl. Acad. Sci. USA,72, 323.
Fox, M. S. (1966)J. Gen. Physiol.,49, 183.
Fox, M. S. and Allen, M. K. (1964)Proc. Natl. Acad. Sci. USA,52, 412.
Goodgal, S. H. (1982)Ann. Rev. Genet.,16, 169.
Goodgal, S. H. and Notani, N. K. (1968)J. Mol. Biol.,35, 449.
Herriott, R. M., Meyer, E. M. and Vogt, M. (1970)J. Bacterial.,101, 517.
Joshi, V. P. and Notani, N. K. (1983)J. Biosci.,5, 339.
Kahn, M. E., Barany, F. and Smith, H. O. (1983)Proc. Natl. Acad. Sci. USA,80, 6927.
Kahn, M. E., Maul, G. and Goodgal, S. H. (1982)Proc. Natl. Acad. Sci. USA,79, 6370.
Kahn, M. E., Concino, M., Gromkova, R. and Goodgal, S. H. (1979)Biochem. Biophys. Res. Commun.,87, 764.
Lacks, S. A. (1962)J. Mol. Biol.,5, 119.
Lacks, S. A. (1966)Genetics,53, 207.
Lacks, S. A. (1977) inMicrobial Interactions, Receptors and Recognition, (ed. J. L. Reissig) (London: Chapman and Hall), p. 179.
Lacks, S., Dunn, J. and Greenberg, B. (1982)Cell,31, 327.
Lacks, S. A., Greenberg, B. and Neuberger, M. (1974)Proc. Natl. Acad. Sci. USA,71, 2305.
Lacks, S. A. and Neuberger, M. (1975)J. Bacteriol.,124, 132.
Lopez, P., Espinosa, M., Stassi, D. L. and Lacks, S. A. (1982) inGenetic Exchange, (eds V. Streips, S. H. Goodgal, W. R. Guild and G. A. Wilson) (New York arid Basel: Marcel and Dekker Inc.) p. 247.
Morrison, D. A. (1977)J. Bacterial,132, 576.
Morrison, D. A. and Baker, M. F. (1979)Nature (London),282, 215.
Mottes, M., Grandi, G. and Sgaramella V. (1979)Mol Gen. Genet.,178, 281.
Notani, N. K. (1971)/Mol. Biol., 59, 223.
Notani,N. K. (1981)J. Biosci.,3, 431.
Notani, N. K., Frankel, F. R. and Goodgal, S. H. (1966)The Physiology of Gene and Mutation Expression, Proc. G. Mendel Memorial Symp. Brno, Czechoslovakia, p. 151.
Notani, N. K. and Goodgal, S. H. (1965)J. Mol Biol.,13, 611.
Notani, N. K. and Goodgal, S. H. (1966)J. Gen. Physiol.,49, 197.
Notani, N. K. and Setlow, J. K. (1972)J. Bacteriol.,112, 751.
Notani, N. K. and Setlow, J. K. (1974)Prog. Nucleic Acid Res. Mol. Biol.,14, 39.
Notani, N. K., Setlow, J. K. and Allison, D. P. (1973)J. Mol. Biol.,75, 581.
Notani, N. K., Setlow, J. K., Joshi, V. R. and Allison, D. P. (1972)J. Bacteriol.,110, 1171.
Notani, N. K., Setlow, J. K., McCarthy, D. and Clayton, N. L. (1981)J. Bacteriol.,148, 812.
Postel, E. H. and Goodgal, S. H. (1972)J. Bacteriol.,109, 292.
Raina, J. L. and Ravin, A. W. (1980)Proc. Natl Acad. Sci. USA,77, 6062.
Scocca, J. J., Poland, R. L. and Zoon, K. L. (1974)J. Bacteriol.,118, 369.
Setlow, J. K., Notani, N. K., McCarthy, D. and Clayton, N. L. (1981)J. Bacteriol.,148, 804.
Setlow, J. K., Spikes, D. and Ledbetter, M. (1984)J. Bacteriol.,158, 872.
Seto, H. and Tomasz, A. (1974)Proc. Natl. Acad. Sci. USA,71, 1493.
Seto, H. and Tomasz, A. (1975)J. Bacteriol.,124, 969.
Singh, R. N. and Pitale, M. P. (1968)J. Bacteriol.,95, 864.
Sisco, K. L. and Smith, H. O. (1979)Proc. Natl. Acad. Sci. USA,76, 972.
Smith, H. O., Danner, D. B. and Deich, R. A. (1981)Ann. Rev. Biochem.,50, 41.
Spizizen, J., Reilly, B. E. and Evans, H. 1966Ann. Rev. Microbiol.,20, 371.
Stassi, D. L., Lacks, S. A., Lopez, P. and Espinosa, M. (1982) inGenetic Exchange, (eds U. Streips, S. H. Goodgal, W. R. Guild and G. A. Wilson) (New York and Basel: Marcel Dekker Inc.) p. 235.
Tomasz, A. (1969)Ann. Rev. Genet.,3, 217.
Tomasz, A. (1970)J. Bacteriol.,101, 860.
Vogt, K. and Goodgal, S. H. (1984a)J. Bacterol, (In Press).
Vogt, K. and Goodgal, S. H. (1984b)J. Bacterol, (In Press).
Zeigler, R. and Tomasz, A. (1970)Biochem. Biophys. Res. Commun.,41, 1342.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Notani, N.K., Joshi, V.P. & Kanade, R.P. Genetic transformation in bacteria. J Biosci 6, 525–533 (1984). https://doi.org/10.1007/BF02703903
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02703903