Abstract
InHaemophilus influenzae genetic transformation for a plasmid marker is significantly increased when recombinant plasmid RSF 0885 DNA carrying chromosomal DNA segments is used instead of the plasmid DNA alone. Chromosomal DNA by itself, added even a few minutes after the addition of plasmid DNA to competent cells, stopped further uptake of the plasmid DNA. These observations are consistent with the idea that plasmid RSF 0885 contains a ‘degenerate’ version of the required eleven base-pair ‘uptake sequence’ inHaemophilus. The transformation activity of the recombinant plasmid DNA is recoverable after its entry into cells, although the specific biological activity of the re-isolated plasmid DNA is less than that of the parental recombinant plasmid DNA. Therec 1 gene function of the host is necessary for obtaining higher transformation frequencies with recombinant DNA from five different clones. The reduced transformation frequencies seen inrec 1 - strain is not all due to a permanent damage to the donor DNA since the recovered recombinant plasmid DNA from such cells can increase the transformation efficiency onrec 1 + strain.
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References
Hirt, B. (1967)J. Mol. Biol.,26, 365.
Lacks, S. (1979)J. Bacteriol.,138, 404.
Marmur, J. (1961)J. Mol. Biol.,3, 208.
McDonell, M. W., Simon, M. N. and Studier, F. W. (1977)J. Mol. Biol.,110, 119.
Notani, N. K. and Goodgal, S. H. (1966)J. Gen. Physiol.,49, 197.
Notani, N. K. (1971)J. Mol. Biol.,59, 223.
Notani, N. K. and Setlow, J. K. (1972)J. Bacteriol.,112, 751.
Notani, N. K., Setlow, J. K. and Allison, D. (1973)J. Mol. Biol.,75, 581.
Radloff, R., Bauer, W. and Vinograd, J. (1967)Proc. Natl. Acad. Sci. USA,57, 1514.
Sisco, K. L. and Smith, H. O. (1979)Proc Natl. Acad. Sci., USA,76, 972.
Steinhart, W. L. and Herriott, R. M. (1968)J. Bacteriol.,96, 1718.
Voll, M. J. and Goodgal, S. H. (1961)Proc. Natl. Acad. Sci., USA,47, 505.
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Notani, N.K. Genetic and molecular events in transformation ofHaemophilus influenzae with plasmid RSF 0885 carrying cloned segments of chromosomal DNA. J Biosci 3, 431–438 (1981). https://doi.org/10.1007/BF02702631
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DOI: https://doi.org/10.1007/BF02702631