Abstract
We have carried out crystal structure analysis of raw pure Mysore silk fibers belonging toBombyx mori on the basis of model parameters of Marshet al using Linked-Atom-Least-Squares technique. The intensity of all the reflections were computed employing CCP13 software. We observe that the molecular modification is essentially same as β-pleated structure with antipolar-antiparallel arrangements formed by hydrogen bonds. The essential differences observed in the structure are highlighted and discussed
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CCP13 2004 Fiber and Polymer Diffraction Software, Daresbury Lab, UKhttp://wserv1.do.ac.uk/SRS/CCP13
Demura M, Minami M, Asakura T and Cross T A 1998 Structure ofBombyx more silk Fibroin based on solid state NMR Orientational Constrains and Fiber Dirrraction unit cell parameters;J. Am. Chem. Soc. 120 1300–1308
Desiraju G R and Steiner T 1999The weak hydrogen bond (New York: Oxford University Press)
Fossey S A, Nemethy G, Gibson K D and Scheraga H A 1991 Conformational energy studies of Beta-Sheets of model silk fibroin peptides. 1. Sheets of poly (Ala-Gly) chains;Biopolymers 31 1529–1541
Hall I H and Pass M G 1976 Chain Conformation of Poly (tetramethyeline terephthalate) and its change with strain;Polymer. 17 807
International Tables for Crystallography 1974 Vol 4 (Kynoch Press: Birmingham) p 71
Ivanova M I and Makowshi L 1998 Iterative Low-Pass Filtering for estimation of the background in Fiber Diffraction patterns;Acta. Crystalogr. A54 626–631
Jeffrey G A 1997An introduction to hydrogen bonding (Oxford: Oxford University Press)
Lucas F, Shaw J T B and Smith S G 1957 The amino acid sequence in a fraction of the fibroin ofBombyx mori;Biochem. J. 66 468–479
Marsh R E, Corey R B and Pauling L 1955 An investigation of the structure of silk fibroin;Biochim. Biopyhys. Acta. 16 1–33
Momany F A, Mcguire R F, Burgess A W and Scheraga H A 1975 Energy parameters in poly peptides. VIII;J. Phys. Chem. 79 2361–2381
Okuyama K, Keiichi Noguch, Takashi Miyazawa, Toshifumi Yui and Kogo Ogawa 1997 Molecular and Crystal structure of hydrated chitosan;Macromolecules 30 5849–5855
Okuyama K, Somashekar R, Noguchi K and Ichimura S 2001 Refined molecular and crystal structure of silk-I Based on Ala-Gly and (Ala-Gly)2-Ser-Gly peptide sequence;Biopolymers 59 310–319
Skilling J and Bryan R K 1984 Maximum entropy image reconstruction-General Algorithm;R. A. S. Monthly Notices 211 111–124
Smith P J C and Arnott S 1978 LALS: A lined atom least squares reciprocal-space refinement system incorporation stereochemical restraints to supplement sparse diffraction data;Acta. Crystalogr. A34 3–11
Spek A L 2003 Single-Crystal structure validation with the program PLATON;J. Appl. Crystalogr. 36 7–13
Squire J, Al-khayat H, Arnott S, Crawshaw J, Denny R, Dover D, Forsyth T, Andrew He, Knupp C, Mant G, Rajakumar G, Rodman M, Shotton M and Windle A 2003 New CCP13 software and strategy behind further developments: Stripping and modelling of fibre diffraction data;Fibre Diffraction Rev. 11 7–19
Strydom D J, Haylett T and Stead R H 1977 The amino-terminal sequence of silk fibroin peptide CP-a reinvenstigation;Biochem. Biophys. Res. Commun. 3 932–938
Takahashi Y, Gehoh M and Yuzuriha K 1999 Structure refinement and diffuse streak scattering of silk(Bombyx mori);Int. J. Bio. Mac. 24 127–138
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Sangappa, Mahesh, S.S. & Somashekar, R. Crystal structure of raw pure Mysore silk fibre based on (Ala-Gly)2-Ser-Gly peptide sequence using Linked-Atom-Least-Squares method. J. Biosci. 30, 259–268 (2005). https://doi.org/10.1007/BF02703707
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DOI: https://doi.org/10.1007/BF02703707