Abstract
Using RNA extracted fromZingiber officinale rhizomes and primers designed according to the conservative regions of monocot mannose-binding lectins, the full-length cDNA ofZ. officinale agglutinin (ZOA) was cloned by rapid amplification of cDNA ends (RACE). The full-length cDNA ofzoa was 746 bp and contained a 510 bp open reading frame (ORF) encoding a lectin precursor of 169 amino acids with a signal peptide. ZOA was a mannose-binding lectin with three typical mannose-binding sites (QDNY). Semi-quantitative RT-PCR analysis revealed thatzoa expressed in all the tested tissues ofZ. officinale including leaf, root and rhizome, suggesting it to be a constitutively expressing form. ZOA protein was successfully expressed inEscherichia coli with the molecular weight expected. To our knowledge, this is the first mannose-binding lectin cDNA cloned from the family Zingiberaceae. Our results demonstrate that monocot mannose-binding lectins also occur within the family Zingiberaceae
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Abbreviations
- AAA:
-
Allium ascalonicum agglutinin
- ACPA:
-
Ananas comosus (pineapple) agglutinin
- AKA:
-
Amorphophallus konjac agglutinin
- APA:
-
Allium porrum agglutinin
- ASA:
-
Allium sativum agglutinin
- AUA:
-
Allium ursinum agglutinin
- CAA:
-
Crinum asiaticum agglutinin
- CHA:
-
Cymbidium hybrid agglutinin
- EHA:
-
Epipactis helleborine agglutinin
- GEAFP:
-
Gastrodia elata antifungal protein
- GNA:
-
Galanthus nivalis agglutinin
- LOA:
-
Listera ovata agglutinin
- NHA:
-
Narcissus hybrid agglutinin
- ORF:
-
open reading frame
- PCR:
-
polymerase chain reaction
- RACE:
-
rapid amplification of cDNA ends
- RT-PCR:
-
reverse transcriptase-polymerase chain rection
- TDA:
-
Typhonium divaricatum agglutinin
- TMA:
-
Taxus media agglutinin
- UTR:
-
untranslated region
- ZGA:
-
Zephyrathes grandiflora agglutinin
- ZOA:
-
Zingiber officinale agglutinin
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Chen, Z., Kai, G., Liu, X. et al. cDNA cloning and characterization of a mannose-binding lectin fromZingiber officinaleRoscoe (ginger) rhizomes. J. Biosci. 30, 213–220 (2005). https://doi.org/10.1007/BF02703701
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DOI: https://doi.org/10.1007/BF02703701